CircBAZ1B stimulates myocardial ischemia/reperfusion injury (MI/RI) by modulating miR-1252-5p/ATF3-mediated ferroptosis by Ruili Wei, Tianxiao Yang, Weihong Li, Xiqian Wang

“…Gene and ferroptosis-related protein expression levels were verified by qRT‒PCR and Western blotting. RNA pull-down, RNA immunoprecipitation (RIP), and a dual-luciferase reporter system were utilized for verification of the molecular interactions. …”
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Digital Education for Students of LBBP D’King Study, Kramat Jati, East Jakarta by Lydia Kurniawan, Henni Handari, Tikkos Sitanggang, Malik Hidayat, Yopie Yulius, Aang Syahdina, Swesti Mahardini

“…The implementation methods included lectures, discussions, Q&A sessions, and training on the use of digital technology in learning activities as an effort to transform education and realize "Merdeka Belajar" (Freedom to Learn). …”
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Pulse current assisted regulation for creep aging behavior of 1420 alloy under different current density by Yuan Liu, Minghao Li, Guoqing Chen, Yufei Zu, Wenlong Zhou, Xuesong Fu

“…The introduction of electrical pulses curtails the activation energy (Q) of the alloy from 93.129 to 86.773 kJ mol⁻1. …”
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Enteric-Coated Aspirin Induces Small Intestinal Injury via the Nrf2/Gpx4 Pathway: A Promising Model for Chronic Enteropathy by Zhang M, Xia S, Feng L, Han X, Zhang Y, Huang Y, Liu Y, Zhao K, Guan J, Tian D, Liao J, Yu Y

“…The mRNA and protein levels of inflammatory factors, tight junctions, and antioxidant defense-related genes were measured by qRT-PCR and Western Blot. The MPO activity, SOD activity and MDA content in serum were measured. …”
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Diverse Profiles of Toll-Like Receptors 2, 4, 7, and 9 mRNA in Peripheral Blood and Biopsy Specimens of Patients with Celiac Disease by Haniye Ghasiyari, Mohammad Rostami-Nejad, Davar Amani, Kamran Rostami, Mohamad Amin Pourhoseingholi, Hamid Asadzadeh-Aghdaei, Mohammad Reza Zali

“…The mRNA expression of TLRs was quantified by relative qPCR with β2 microglobulin (β2m) as a reference gene. …”
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Design of an Iterative Model for Incremental Enhancements in Quantum Image Processing Using Reinforcement Learning-Based Optimizations by Lalitha Kumari Pappala, Sai Babu Veesam, Kannaiah Chattu, Janapati Venkata Krishna, Jyostna Devi Bodapati, B. Tarakeswara Rao

“…There are four key methodologies that have been proposed in this work: Quantum Support Vector Machines with Enhanced Feature Extraction, Quantum Generative Adversarial Networks for Data Augmentation, Quantum Entanglement and Correlation Analysis for Feature Detection, and Quantum-Enhanced Image Reconstruction for Noisy Data Samples. Q-SVM-EFE is a Quantum Support Vector Machine-based framework that incorporates advanced feature extraction to map classical image data into high-dimensional quantum states, which should capture intricate patterns and therefore be more accurate for classification. …”
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Controlled ovarian hyperstimulation with or without letrozole for fertility preservation in breast cancer patients: study protocol for a randomised controlled trial by Ping Li, Kun Wang, Qi Fan, Peng Sun, Yuhua Shi, Yanqiu Xie, Weifen Deng, Jiajing Kang

“…The secondary outcomes are the number of high-quality embryos, incidence of ovarian hyperstimulation syndrome(OHSS) and recurrence rate of breast cancer.Ethics and dissemination Ethical approval was obtained from the Ethics Review Committee of Guangdong Provincial People’s Hospital (KY-Q-2023-840-02). Written informed consent will be obtained from each participant. …”
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Methylsulfonylmethane (MSM) Supplementation in Adult Horses Supports Improved Skeletal Muscle Inflammatory Gene Expression Following Exercise by Madison R. Barshick, Kristine M. Ely, Keely C. Mogge, Lara M. Chance, Sally E. Johnson

“…A comparison of the exercise by diet contrasts revealed that horses supplemented with MSM contained a greater number of exercise-responsive genes (630; logFC > 0.2; q < 0.05) by comparison to the CON (237), with many of these mapping to the immune response (71) and cytokine signal transduction (60) pathways. …”
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DMAV in Drinking Water Activated NF-κB Signal Pathway and Increased TGF-β and IL-1β Expressions in Bladder Epithelial Cells of Rats by Siqi Cao, Shengnan Liu, Fei Wang, Jieyu Liu, Mengdan Li, Chen Wang, Shuhua Xi

“…In this study, we measured the expressions of mRNA and proteins of NF-κB pathway members, IKKα, IKKβ, p65, and p50 in rat bladder epithelium by qRT-PCR and immunohistochemical analysis after rats received drinking water containing 100 and 200 ppm DMAV for 10 weeks. …”
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Meta-analysis of nifedipine and enalapril combination therapy for hypertensive patients with coronary heart disease: A systematic review and meta-analysis by Kun Wang, Wenchao Ma, Leina Sun, Fangcheng Su

“…Heterogeneity in the studies was evaluated based on the results of the Q test (P value), and the OR value of the combined effect was calculated using either the model with fixed effects or the one with random effects, with the results presented in a forest plot. …”
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Enucleated bone marrow-derived mesenchymal stromal cells regulate immune microenvironment and promote testosterone production through efferocytosis by Lu Sun, Jiayu Huang, Xuezi Wang, Peng Huang, Baolin Dong, Zehang Liang, Jiahong Wu, Jiancheng Wang

“…To determine the mechanism of efferocytosis, we analysed the number of macrophages by immunofluorescence staining and quantitative real-time polymerase chain reaction (qRT-PCR). Results The injection of enucleated BMSCs (Cargocytes) into the testes of EDS-treated rats restored the levels of serum testosterone, increased the number of Leydig cells (LCs), and improved spermatogenesis. …”
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Exosomal PVRL4 Promotes Lung Adenocarcinoma Progression by Enhancing the Generation of Myeloid‐Derived Suppressor Cell‐Secreted TGF‐β1 by Yahai Liang, Jinmei Li, Lihua Zhang, Jinling Zhou, Meilian Liu, Xiaoxia Peng, Weizhen Zheng, Zhennan Lai

“…Methods Detection of levels of mRNAs and proteins was conducted using qRT‐PCR and western blotting. The CCK‐8, colony formation, transwell, wound healing assays, and flow cytometry were used to explore cell growth, invasion, migration, and apoptosis, respectively. …”
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Clinical characteristics of and growth hormone treatment effects on short stature with type 1 insulin-like growth factor receptor (IGF1R) gene alteration by Yuki Kawashima-Sonoyama, Keisuke Wada, Kei Yamamoto, Masanobu Fujimoto, Noriyuki Namba, Takeshi Taketani

“…Four cases were caused by a deletion of chromosome 15q26.3, and eight were caused by heterozygous pathogenic variants in the IGF1R gene. …”
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Mind the leaf anatomy while taking ground truth with portable chlorophyll meters by Zuzana Lhotáková, Eva Neuwirthová, Markéta Potůčková, Lucie Červená, Lena Hunt, Lucie Kupková, Petr Lukeš, Petya Campbell, Jana Albrechtová

“…Optical Chl assessments included measurements taken by four chlorophyll meters: three transmittance-based (SPAD-502, Dualex-4 Scientific, and MultispeQ 2.0), one fluorescence-based (CCM-300), and vegetation indices calculated from the 400–2500 nm leaf reflectance acquired using an ASD FieldSpec and a contact plant probe. …”
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Development and Validation of an Ion-Pair HPLC-UV Method for the Quantitation of Quinoline and Indoloquinoline Alkaloids in Herbal and Pharmaceutical Antimalarial Formulations by Samuel Oppong Bekoe, Emmanuel Orman, Samuel Awenteka Adjabui, Abena Amponsaa Brobbey, James Oppong-Kyekyeku, Kwabena Frimpong-Manso Opuni, Noble Kuntworbe, Samuel Asare-Nkansah

“…Method validation was according to ICH Q2(R1) guidelines. It was then used to assess the quality of APIs (n = 3) and HPAFs (n = 44) including quinine-based pharmaceutical antimalarial formulations (QBPAFs) (n = 23) and herbal antimalarial products (HAMPs). …”
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Regulatory mechanism of exogenous myo-inositol improving high temperature stress tolerance of tomato seedlings by CHEN Yan, HAN Liyuan, DU Zilong, ZHU Dongxing, ZHU Weimin, ZHANG Yingying

“…The expression of heat responsive genes, antioxidant, and ABA-related genes were analyzed by qRT-PCR. [Results] 20 mmol/L myo-inositol treatment could effectively alleviate the damage of high temperature stress on tomato seedlings, reduce the heat damage index of tomato leaves, increase the maximum photochemical efficiency (Fv/Fm), and significantly increased the content of photosynthetic pigments. …”
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Astaxanthin Protects Primary Hippocampal Neurons against Noxious Effects of Aβ-Oligomers by Pedro Lobos, Barbara Bruna, Alex Cordova, Pablo Barattini, Jose Luis Galáz, Tatiana Adasme, Cecilia Hidalgo, Pablo Muñoz, Andrea Paula-Lima

“…Longer exposures to AβOs (6 h) promoted NFATc4-eGFP nuclear translocation and decreased RyR2 mRNA levels, evaluated by detection of the eGFP-tagged fluorescent plasmid and qPCR, respectively. Preincubation with 0.1 μM ATX prevented both effects. …”
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Cell-free DNA release following psychosocial and physical stress in women and men by A. S. Limberg, F. Berg, E. Köper, C. Lindgraf, C. Gevers, R. Kumsta, E. M. Hummel, D. A. Moser

“…To overcome this, a multiplex quantitative polymerase chain reaction (qPCR) protocol targeting L1PA2 elements, which are prevalent in the human genome, was utilized. …”
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Pan-Cancer Analysis of Disulfidptosis-Related Genes Affecting Prognosis and Tumor Microenvironment by Jingyang SUN, Rongxuan JIANG, Liren HOU, Huanhuan DONG, Yihan LIN, Niuniu DONG, Guangjian ZHANG, Yanpeng ZHANG

“…The expression levels of DRGs at the cellular level were experimentally validated using qPCR. ResultsLRPPRC, NCKAP1, NDUFS1, and NUBPL had a better prognosis in renal clear cell carcinoma (P<0.001), whereas SLC7A11, NCKAP1, and SLC3A2 had a worse prognosis in hepatocellular carcinoma (P<0.001). …”
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Comparison of miRNA expressions among benign, premalignant and malignant lesions of the larynx: could they be transformation biomarkers? by Fatma Ruya Tuncturk, Ibrahim Akalin, Lokman Uzun, Tulay Zenginkinet

“…Materials and methods We investigated mature miRNA expressions in paraffin-embedded surgical specimens of human laryngeal tissues grouped as benign, premalignant or malignant (n = 10 in each). miRNA profiling was carried out by quantitative Real-Time polymerase chain reaction (RT-qPCR) and data were analyzed according to fold regulation. …”
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