Qufeng epimedium decoction alleviates rheumatoid arthritis through CYLD-antagonized NF-kB activation by deubiquitinating Sirt1 by Zhiming Wu, Peng Zhang, Wenyan Huang, Yifen Zhou, Zhengliu Cao, Chunhong Wu

“…Methods: The RA animal model was established to investigate the anti-RA effect and regulatory effect on fibroblast-like synoviocytes (FLS) pyroptosis, qRT-PCR, Western blot, flow cytometry, histology staining, and ELISA were utilized to confirm the gene and protein expressions. …”
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Two Cysteines in Raf Kinase Inhibitor Protein Make Differential Contributions to Structural Dynamics In Vitro by Hyun Sang Cho, Mohammad Faysal Al Mazid, Eun-Young Lee, Md Abu Rayhan, Hyoun Sook Kim, Byung Il Lee, Hye Jin You

“…Mutation of alanine or serine instead of cysteine in RKIP proteins did not affect the biochemical characteristics, while dynamic light scattering and liquid chromatography (LC) quadrupole time-of-flight (Q-TOF) mass spectrometry (MS) suggested distinct peaks in solution, which were identified via LC–MS/MS analyses, and further clarified the role of cysteine in RKIP dimerization. rhRKIP dimer formation was abrogated by a 32-aa peptide mimicking the region between two RKIP proteins for dimerization. …”
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Diabetes Mellitus Is Associated with a Lower Risk of Gout: A Meta-Analysis of Observational Studies by Xiaoli Li, Lianju Li, Yuling Xing, Tiantian Cheng, Shaohui Ren, Huijuan Ma

“…The multivariate adjusted relative risks (aRR) and corresponding 95% confidence intervals (CI) were pooled based on a random-effect model. Cochran’s Q test and I2 were used to evaluate heterogeneity. …”
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Phenylbutyric Acid Modulates Apoptosis and ER Stress‐Related Gene Expression in Glycogen Storage Disease Type Ib In Vitro Model by Marina Parezanovic, Nina Stevanovic, Marina Andjelkovic, Milena Ugrin, Sonja Pavlovic, Maja Stojiljkovic, Anita Skakic

“…Molecular markers of unfolded protein response (ATF4, DDIT3, HSPA5, XBP1s), and apoptosis (BCL2/BAX, CASP3, CASP7) in G6PT‐deficient cells were analyzed using RT‐qPCR method before and upon the treatment with 4‐PBA. …”
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Phenolic compounds from Chaenomeles speciosa alleviate inflammation in lipopolysaccharide-treated RAW264.7 macrophages via the NF-κB and MAPK pathways by Fuxia Hu, Chao Liu, Fengqin Wang, Changxin Zhou, Maotong Zhu, Dongxiao Sun-Waterhouse, Zhaosheng Wang

“…In this study, a purified phenolic extract of C. speciosa rich in chlorogenic acid, procyanidin B1 and catechin (determined by HPLC-Q-TOF-MS/MS) exhibited dose-dependent anti-inflammatory effects on lipopolysaccharide (LPS)-treated RAW264.7 macrophages. …”
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Mechanism of mTOR/RILP-regulated autophagic flux in increased susceptibility to myocardial ischemia-reperfusion in diabetic mice by Jiyao Zhao, Wei Shi, Yan Zheng, Junjie Wang, Muzhao Yuan, Yultuz Anwar, Yuxuan He, Haiping Ma, Jianjiang Wu

“…Transmission electron microscopy revealed that the accumulation of autophagic vesicles in cardiomyocytes of diabetic mice resulted in impaired autophagic flux. qRT-PCR revealed that the expression of RILP was significantly reduced in diabetic mice subjected to I/RI. …”
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Stemness-related gene signatures as a predictive tool for breast cancer radiosensitivity by Jinzhi Lai, Rongfu Huang, Jingshan Huang

“…Key genes identified in the radiosensitivity signature were validated in vitro by qRT-PCR.ResultsBy analyzing gene expression data from 920 BRCA samples, we identified a set of 267 stemness-related genes between high and low mRNAsi groups. …”
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circSLC6A6 Sponges miR-497-5p to Promote Endometrial Cancer Progression via the PI4KB/Hedgehog Axis by Juan Hu, Xing Peng, Weina Du, Yichuan Huang, Chun Zhang, Xian Zhang

“…This study was aimed at investigating the functional roles of circSLC6A6 in EC. Methods. The qRT-PCR assay was performed to detect the circSLC6A6 expression in EC tissues and cell lines. …”
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Total Glucosides of Paeony Alleviate Cell Apoptosis and Inflammation by Targeting the Long Noncoding RNA XIST/MicroRNA-124-3p/ITGB1 Axis in Renal Ischemia/Reperfusion Injury by Fang Chen, Yi Hu, Yuetao Xie, Zonghui Zhao, Lin Ma, Zhili Li, Wanlong Tan

“…The lncRNA XIST (XIST) expression was analyzed by qRT-PCR. Then, the viability and apoptosis of renal cells were detected by MTT and flow cytometry assay. …”
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The Spatio-temporal Evolution of NDVI in the Three Gorges Reservoir Area Driven by the Synergistic Effect of two Factors over the Past 21 Years by QI Haimei, ZHENG Peilong, WANG Yunqi, WANG Jiani, LI Cheng, ZHANG Xiaoming

“…[Results] (1) The change of NDVI in the Three Gorges Reservoir Area showed an overall ascending trend from 2000 to 2020, with a mean annual rate of increase at 2.89×10-3/a, and the value of NDVI increased from the southwestern to the northeastern parts of the area. (2) Elevation, population density, and surface temperature are pivotal factors that significantly account for the variability observed in NDVI, with an explanatory power for each exceeding the threshold of 0.4. (3) Elevation and nocturnal illumination are the key interactive drivers of NDVI in the Three Gorges Reservoir Area, with a pronounced q value of 0.641. Their interplay showed a nonlinear or synergistic enhancement, and the integrated explanatory power of the two factors on NDVI changes is always greater than that of single factors. …”
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Radiation decreases bronchial epithelial progenitor function as assessed by organoid formation by Merian E. Kuipers, Dennis K. Ninaber, Krista C.J. van Doorn-Wink, Annelies M. Slats, Pieter S. Hiemstra

“…Effect of IR (1, 2, 4, 8 Gy [Gy]) on ALI-PBEC cultures was investigated by lactate dehydrogenase (LDH) release, Trans Epithelial Electrical Resistance (TEER; as a measure of barrier function), qPCR (P21/CDKNA1, MKI67, AEN, E2F1, ATF3) and immunofluorescence staining (γH2Ax-foci). …”
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Manifestation of agronomically valuable traits in the progeny of a sorghum mutant carrying the genetic construct for RNA silencing of the γ-kafirin gene by L. A. Elkonin, N. V. Borisenko, T. E. Pylaev, O. A. Kenzhegulov, S. Kh. Sarsenova, N. Yu. Selivanov, V. M. Panin

“…In T2, a line with monogenic control of GA resistance was selected. qPCR analysis showed that in different T3 and T4 plants, the genetic construct was present in 2–4 copies. …”
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Under simulated microgravity and gravity, anthocyanin is regulated by DcaWRKY2 in Dendrobium catenatum leaves by Tianze Hou, Baoqiang Zheng, Fucheng Peng, Zehui Jiang, Wenbo Zhang, Yan Wang

“…Furthermore, based on the transcriptome sequencing, differentially expressed genes (DEGs), and weighted gene co-expression network analysis combined with RT-qPCR, we identified one WRKY gene, DcaWRKY2, from a Dendrobium catenatum under simulated microgravity conditions, which indicated that DcaWRKY2 may be involved in anthocyanin biosynthesis under simulated microgravity conditions. …”
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The role of TRECs/KRECs as immune indicators that reflect immunophenotypes and predict the risk of infection in systemic autoimmune diseases by Takuji Itakura, Hirokazu Sasaki, Tadashi Hosoya, Natsuka Umezawa, Tetsuya Saito, Hideyuki Iwai, Hisanori Hasegawa, Hiroyuki Sato, Akihiro Hirakawa, Kohsuke Imai, Tomohiro Morio, Naoki Kimura, Shinsuke Yasuda

“…To clarify the significance of TRECs and KRECs as immune indicators in patients with systemic autoimmune diseases, we prospectively evaluated TREC and KREC levels with qPCR, lymphocyte phenotypes with flow cytometry, along with lymphocyte counts and serum immunoglobulin levels in peripheral blood samples from newly diagnosed patients. …”
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Differences in central dopamine, but not serotonin, activity and welfare associated with age but not with preening cup use in commercial grow-out Pekin duck barns by M.M. Bergman, J.M. Schober, R. Novak, A. Grief, C. Plue, D. Frey, H. Parnin, G.S. Fraley

“…To evaluate the ducks’ affective state, we combined measuring welfare by walking a transect in the barn with mass spectrometry and qRT-PCR to measure brain neurotransmitter levels and gene expression for serotonin (5-HT) and dopamine (DA) synthesis and metabolism. 5-HT and DA have been established as indicators of mental state and emotions. …”
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Estimating the impact of the Mongol expansion upon the gene pool of Tuvans by L. D. Damba, Е. V. Balanovskaya, M. K. Zhabagin, Y. М. Yusupov, Y. V. Bogunov, Z. M. Sabitov, A. T. Agdzhoyan, N. A. Korotkova, M. B. Lavryashina, B. B. Mongush, U. N. Kavai-ool, O. P. Balanovsky

“…On average, two-thirds of their gene pools (63 %) are composed by North Eurasian haplogroups (N*, N1a2, N3a, Q) connected with autochtonous populations of modern area of Tuvans. …”
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Tetrastigma hemsleyanum as a feed additive: modulating gut microbiota for enhancing nutritional transport and growth performance in Jinhua yellow chickens by Chao Lu, Yun Xiang, Kewei Xu, Fengrui Gao, Shaofeng Zhu, Fangfang Lou, Lu Liu, Xin Peng

“…Additionally, TH supplementation notably increased gut microbiota richness, then selectively increased the colonization of potential probiotics and the microbial abundance associated to amino acid synthesis and metabolic pathways in duodenum. Furthermore, qPCR analysis results preliminarily verified that dietary TH not only enhanced intestinal amino acids (rBAT and EAAT3) and peptides (PepT1 and APN) transport but also alleviated the inflammation (IL-1β, IL-6 and IFN-γ), thus thereby improved intestinal development and growth performance in Jinhua yellow chickens. …”
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Circulating miRNAs as Biomarkers for Prostate Cancer Diagnosis in Subjects with Benign Prostatic Hyperplasia by Wei Jin, Xiang Fei, Xia Wang, Fangjie Chen, Yan Song

“…In total, 31 PCa subjects and 31 BPH subjects were included, with the BPH group serving as the control group. RT-qPCR was used to quantify the levels of 10 miRNAs, which included miR-18a, miR-34a, miR-106b, miR-183, miR-200a, miR-301a, miR-141, miR-182, miR-200b, and miR-375 in serum. …”
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Integrated analysis of transcriptome, sRNAome, and degradome involved in the drought-response of maize Zhengdan958 by Yang Shuqiong, Liu Jiafei, Cao Lingling, Chen Jibao, Duan Pengfei

“…The integrated multi-omics analysis revealed that five DEmiRNA–mRNA pairs displayed negatively correlated expression patterns, which were also verified by qRT-PCR. Tissue-specific expression profile and regulatory network analysis revealed that miR528a/b-Zm00001d021850, miR408a/b-Zm00001d020794, and miR164e-Zm00001d003414 might be essential in root-specific drought stress response of maize Zhengdan958 seedlings. …”
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Fabrication of Core-Shell PEI/pBMP2-PLGA Electrospun Scaffold for Gene Delivery to Periodontal Ligament Stem Cells by Qiao Xie, Lie-ni Jia, Hong-yu Xu, Xiang-gang Hu, Wei Wang, Jun Jia

“…Moreover, the core-shell electrospun scaffold showed BMP2 expression for a much longer time (more than 28 days) compared with the single axial electrospun scaffold, as evaluated by qRT-PCR and western blot after culturing with hPDLSCs. …”
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