Prediction of Seven Artificial Intelligence-Based Intraocular Lens Power Calculation Formulas in Medium-Long Caucasian Eyes by Wiktor Stopyra, Oleksiy Voytsekhivskyy, Andrzej Grzybowski

“…Prior to phacoemulsification, IOL power was computed using SRK/T, Holladay1, Haigis, Holladay 2, and Hoffer Q. The refraction was measured three months after cataract surgery. …”
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Effects of Compressive and Tensile Strain on Macrophages during Simulated Orthodontic Tooth Movement by Agnes Schröder, Paul Käppler, Ute Nazet, Jonathan Jantsch, Peter Proff, Fabian Cieplik, James Deschner, Christian Kirschneck

“…We determined cell number, cytotoxicity, and gene/protein expression of Vegf-a/VEGF-A (macrophage-mediated angiogenesis), Mmp-8/9 (extracellular matrix reorganization), and Cox-2/PG-E2, Il-6/IL-6, and Tnf-α/TNF-α (proinflammatory mediators) by RT-qPCR and ELISA. Compressive but not tensile strain resulted in a significant reduction in cell number after only 2 h. …”
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fMLP-Induced IL-8 Release Is Dependent on NADPH Oxidase in Human Neutrophils by María A. Hidalgo, María D. Carretta, Stefanie E. Teuber, Cristian Zárate, Leonardo Cárcamo, Ilona I. Concha, Rafael A. Burgos

“…IL-8 was measured by ELISA, IL-8 mRNA by qPCR, and ROS production by luminol-amplified chemiluminescence, reduction of ferricytochrome c, and FACS. …”
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Genome-Wide Identification of miRNAs in Oily Persimmon (<i>Diospyros oleifera</i> Cheng) and Their Functional Targets Associated with Proanthocyanidin Metabolism by Meng Zhang, Rong Wu, Xinlong Hu, Zhengrong Luo, Qinglin Zhang, Sichao Yang

“…The promoter elements of the miRNA genes were predicted on the promoter-2.0 software, which indicated that the abundant <i>cis</i>-acting elements were light responsiveness, promoter, and enhancer regions. The qRT-PCR assay was performed to elucidate the potential expression patterns of precursor miRNA and their targets during fruit development, and one target gene, <i>DkMYB22</i>, of miR2911 was verified to promote the conversion of soluble tannins into insoluble tannins involved in the deastringency in persimmons. …”
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Koumine Attenuates Neuroglia Activation and Inflammatory Response to Neuropathic Pain by Gui-Lin Jin, Sai-Di He, Shao-Mei Lin, Li-Mian Hong, Wan-Qing Chen, Ying Xu, Jian Yang, Su-Ping Li, Chang-Xi Yu

“…Western blot analysis and quantitative real-time polymerase chain reaction (qPCR) were used to examine the modulator effect of koumine on microglial M1 polarization. …”
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Negative association between 15 obesity- and lipid-related indices and testosterone in adult males: a population based cross-sectional study by Wei Guo, Shuo Zhao, Qinzheng Chang, Jiajia Sun, Yidong Fan, Jikai Liu

“…After accounting for all covariates, the analysis revealed that individuals within the highest quartile (Q4) for metabolic score for visceral fat (METS-VF) had the excellent probability of developing TD (OR = 13.412, 95%CIs: 4.222, 42.262, P < 0.001). …”
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HTR1D regulates the PI3K/Akt signaling pathway to impact hepatocellular carcinoma development and resistance to sorafenib by Yingai Zhang, Yuting Zhang, Shuai Zhou, Mujeeb Ur Rehman, Fankai Lin, Jianquan Zhang, Hailong Zhou

“…Methods First, the correlation between HTR1D and hepatocellular carcinoma was analyzed using the TCGA database, and the expression level of HTR1D in clinical samples was detected by qPCR. Then the siRNA was transfected into Huh-7 and Hep3B cells, and the cell proliferation ability, colony formation ability, migration and invasion ability were detected with or without sorafenib. …”
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Cefazolin Alone Versus Cefazolin With Tobramycin or Gentamicin as Intraoperative Antibiotic Prophylaxis for Total Joint Arthroplasty by Meera M. Dhodapkar, MD, MHS, Scott J. Halperin, MD, MHS, Zachary Radford, MD, Lee E. Rubin, MD, FAAOS, FAOA, Jonathan N. Grauer, MD, Mengnai Li, FAAOS, MD, PhD

“…Methods: Adult primary THA and TKA patients were identified from 2010-2021 Q1 PearlDiver M151 database. Inclusion criteria were activity in the database ≥ 90 days postoperative and no infectious, neoplastic, or traumatic diagnoses within 90 days preoperative. …”
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RNA-Sequence Reveals the Regulatory Mechanism of miR-149 on Osteoblast Skeleton under Mechanical Tension by Yifan Wang, Guanyin Zhu, Fang Pei, Yigan Wang, Jun Liu, Caixia Lu, Zhihe Zhao

“…Real-time quantitative polymerase chain reaction (RT-qPCR) detected the expression of miR-149 and ARFGAP with SH3 domain, Ankyrin repeat, and Ph domain 3 (ASAP3) mRNA. …”
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Identification of Hepatocellular Carcinoma Subtypes Based on Global Gene Expression Profiling to Predict the Prognosis and Potential Therapeutic Drugs by Cunzhen Zhang, Jiyao Wang, Lin Jia, Qiang Wen, Na Gao, Hailing Qiao

“…Survival-related genes were identified using Cox regression, and their expression and function were validated via qPCR and gene interference. GO, KEGG, GSVA, and GSEA were used to determine enriched signaling pathways. …”
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Isolation and identification of novel casein-derived bioactive peptides and potential functions in fermented casein with Lactobacillus helveticus by Mengzhu Fan, Tingting Guo, Wanru Li, Jing Chen, Fushuo Li, Chao Wang, Yi Shi, David Xi-an Li, Shaohui Zhang

“…The product peptide DELQDKIHPF found in both extracell and intracell was quantitatively analyzed using the MRM mode of UPLC-U3Q, 23.1 and 9.76 ng/mL, respectively. The quantitative analysis of the potential bioactive peptide may also advance the production of peptide products in the future. …”
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Rapid Detection of microRNA-122 in Serum and Finger Blood Using a Lateral Flow Nucleic Acid Biosensor by Min Zhang, Meijing Ma, Jiahui Wang, Yurui Zhou, Xueji Zhang, Guodong Liu

“…Traditional methods for detecting miR-122 mainly include Northern blotting and qRT-PCR, but they are technically complex and cumbersome, requiring expensive instruments and high technical requirements. …”
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Blue applicability grade index assessment and response surface modelling to synchronous determination of metformin hydrochloride, vildagliptin and dapagliflozin propanediol monohyd... by Pintu Prajapati, Krutika Patil, Dhrumi Naik, Veera Shakar Pulusu, Anzarul Haque, Mohd Abul Kalam, Shailesh Shah

“…The chromatographic method was validated as per ICH Q2 (R2) guideline. The developed method was applied for synchronous assay of multiple combinations of metformin, vildagliptin and dapagliflozin using single chromatographic condition to save time, cost and resources for analysis. …”
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Deficiency of the palmitoyl acyltransferase ZDHHC7 modulates depression-like behaviour in female mice after a mild chronic stress paradigm by Christa Hohoff, Nicole Kerkenberg, Mingyue Zhang, Weronika Palkowska, Lydia Wachsmuth, Maja Peng, Lena Stiehl, Christiane Schettler, Johannes C. S. Zang, Andreas Huge, Evgeni Ponimaskin, Cornelius Faber, Bernhard T. Baune, Weiqi Zhang

“…Furthermore, female KOs showed increased cortistatin expression after CS (C vs. CS: mRNAseq and qPCR p all  < 0.05). In sum, Zdhhc7-deficiency reduced depression-like behaviours, prevented hippocampal fibre reduction and upregulated cortistatin after CS. …”
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Metagenomic analysis of DNA viruses from posttransplant lymphoproliferative disorders by Vikas R. Dharnidharka, Marianna B. Ruzinova, Chun‐Cheng Chen, Priyanka Parameswaran, Harry O'Gorman, Charles W. Goss, Hongjie Gu, Gregory A. Storch, Kristine Wylie

“…In univariable analysis, death within 5 years of PTLD diagnosis was associated with anellovirus (P = 0.037) and gammapapillomavirus (P = 0.036) detection by MSS, higher tissue qPCR levels of the predominant human anellovirus species torque teno virus (TTV; P = 0.016), T cell type PTLD, liver, brain or bone marrow location. …”
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Association between systemic inflammatory response index and glaucoma incidence from 2005 to 2008 by Xiang Li, Yi Qing Sun, Xiao Dan Zhong, Zhi Jie Zhang, Jia Feng Tang, Zhan Yang Luo

“…We performed an in-depth analysis of the Log2SIRI quartiles and found a significant association between Log2SIRI Q4 and the occurrence of glaucoma (Model 3: OR1.62, 95%CI 1.12–2.34, p = 0.011). …”
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The biological control agent for bacterial canker of kiwifruit, in Aureo® Gold, is a strain of Aureobasidium pullulans identifiable by novel SCAR marker primers by Deirdre A. Cornish, Magan M. Schipper, Jenny M. Oldham, Janet Yu, Joel L. Vanneste

“…This assay can be used for quality control; however, for detection and quantification of CG163 we developed a quantitative polymerase chain reaction (qPCR) assay. Using this assay, we positively identified CG163 and followed its establishment on kiwifruit leaves for seven days post treatment with Aureo Gold. …”
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Single Nucleotide Polymorphisms in TLR4 Affect Susceptibility to Tuberculosis in Mexican Population from the State of Veracruz by Enrique Ortega, Sujhey Hernández-Bazán, Beatriz Sánchez-Hernández, Ileana Licona-Limón, Javier Fuentes-Dominguez

“…We did not find an association of SNPs in TLR1 (N248S), TLR2 (R753Q), TLR6 (S249P), and TLR10 (A153S and V298I) with tuberculosis disease. …”
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Development of a duplex real-time recombinase aided amplification assay for the simultaneous and rapid detection of PCV3 and PCV4 by Renjie Sun, Hanze Liu, Siqi Sun, Yating Wang, Ying Shan, Xiaoliang Li, Weihuan Fang, Yongle Yang, Ronghui Xie, Lingyan Zhao

“…Comparative analysis with PCV3 and PCV4 qPCR on 60 clinical samples and artificially spiked samples indicated high congruence (the kappa value was 0.966 and 1, respectively, with p < 0.001), with only minor discrepancies, validating effectiveness of the duplex RAA assay in detecting co-infections and its suitability for preliminary clinical diagnosis of PCV3 and PCV4. …”
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Mechanism and Pharmacodynamic Substance Basis of Raw and Wine-Processed Evodia rutaecarpa on Smooth Muscle Cells of Dysmenorrhea Mice by Yeqian Liu, Hong Li, Lei Chen, Hongxia Zhao, Jian Liu, Shan Gong, Danfeng Ma, Chunming Chen, Shuiqing Zeng, Hongping Long, Weiqiong Ren

“…Metabolomics methods based on UPLC-Q-TOF-MS were utilized to analyse the differential components of ER before and after wine processing. …”
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