Ferrostatin-1 inhibits osteoclast differentiation and prevents osteoporosis by suppressing lipid peroxidation by Wenbo Xu, Shiyan Lv, Xiaoyan Wang, Chengchao Song, Chunyang Xi, Jinglong Yan

“…The levels of osteoclastogenesis and lipid peroxides were measured by qRT-PCR, Western Blot and immunofluorescence. Activation of the p38/JNK/MAPK pathway was detected by Western Blot. …”
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Clinical efficacy of various resuscitation fluids in the management of sepsis in postoperative surgical and trauma patients: a systematic review and meta‑analysis by Yongjie Wang, Kewu Chen, Xiaolu Li, Jianing Guan

“…Heterogeneity was assessed using the Cochran Q, I2 statistic, and the appropriate P value. …”
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Indoxyl Sulfate Induces Mesangial Cell Proliferation via the Induction of COX-2 by Shuzhen Li, Sijie Cheng, Zhenzhen Sun, Harr-keshauve Mungun, Wei Gong, Jing Yu, Weiwei Xia, Yue Zhang, Songming Huang, Aihua Zhang, Zhanjun Jia

“…COX-2 expression was examined by Western blotting and qRT-PCR, and a specific COX-2 inhibitor NS398 was applied to define its role in IS-induced MC proliferation. …”
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Integrated bioinformatics and experimental analysis of CHAF1B as a novel biomarker and immunotherapy target in LUAD by Wei Du, Xiao-Wei Wu, Qing-Feng Li, Bing-Yu Zhang, Jing Wu, Ya-Ping Xu, Xue Yi

“…Preliminary in vitro studies using qRT-PCR, CCK8, Transwell, glucose, and lactate metabolism confirmed CHAF1B's expression and role in LUAD. …”
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Streaming Torque with Turbulent Diffusion by Qiang Hou, Cong Yu

“…We found that the dust-induced “Streaming Torque” (ST) may slow down or even reverse the planet migration in Q. Hou & C. Yu. But in realistic protoplanetary disks, dust diffusion induced by gas turbulence may have important influences on ST. …”
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A multi-strain human skin microbiome model provides a testbed for disease modeling by Angela L. Maloney, Tyler Crawford, Jordan Hurlbut, Monica Martinez, Thomas J. Mulhern, Elizabeth L. Wiellette, Else M. Vedula, Vidhya Vijayakumar

“…Up to five of the six strains were viable on the skin tissue surface on day 7 as validated by custom qPCR assays, demonstrating a robust and stable testbed for microbiome studies. …”
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Electroacupuncture regulates ferroptosis to improve postoperative cognitive dysfunction in mice through mediating GRX1/GSK-3β/Nrf2 axis by Xiaqing Zhang, Afen Zhang, Rui Li, Mingna Jiao, Rou Wen, Rongzhi Zheng

“…HE staining was used to examine cell arrangement and cell morphology in hippocampus of mice. RT-qPCR, western blot and IHC were employed to detect abundance of genes and proteins. …”
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Genome-wide identification and expression analysis of CBF/DREB1 gene family in Medicago sativa L. and functional verification of MsCBF9 affecting flowering time by Jing Cui, Yajing Li, Hao Liu, Xu Jiang, Lili Zhang, Hongbo Dai, Xue Wang, Fei He, Mingna Li, Junmei Kang

“…The expression pattern of MsCBF9 under different hormone treatments was verified by RT-qPCR, and it was found that MsCBF9 responded to GA3, IAA, SA, and MeJA. …”
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The Mutual Inhibition of FoxO1 and SREBP-1c Regulated the Progression of Hepatoblastoma by Regulating Fatty Acid Metabolism by Yu Hu, Hongyan Zai, Wei Jiang, Zhenglin Ou, Yuanbing Yao, Qin Zhu

“…FoxO1, SREBP-1c, FASN, ACLY, ACC, and MAGL expressions in tissue samples were detected by RT-qPCR and WB. IHC was utilized to measure FASN content. …”
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Anaerobic metabolism promotes breast cancer survival via Histone-3 Lysine-18 lactylation mediating PPARD axis by Ying Xu, Weiwei Meng, Yingqi Dai, Lin Xu, Ning Ding, Jinqing Zhang, Xuewei Zhuang

“…We utilized chromatin immunoprecipitation sequencing with H3K18la antibodies, transcriptomics of clinical BC samples, and proteomics and ATAC-seq analyses of in vivo tumors to identify the genes regulated by H3K18la and the transcription factor PPARD. qPCR and Western blot assays were used to detect expressions of molecules. …”
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Physiological and molecular mechanisms of exogenous salicylic acid in enhancing salt tolerance in tobacco seedlings by regulating antioxidant defence system and gene expression by Xiliang Song, Jian Chen, Can Xu, Xianjie Cai, Wenjing Song, Aixia Chang, Yu Zhang, Chenggang Luo

“…Exogenous SA also increased ascorbate and glutathione levels and reduced their reduced forms by increasing the activities of glutathione reductase, ascorbate peroxidase, monodehydroascorbate reductase and dehydroascorbate reductase. qRT−PCR analysis revealed that the key genes regulating SA biosynthesis, carbon assimilation, the antioxidant system and the ascorbate−glutathione cycle were activated by SA under conditions of salt stress.DiscussionOur study elucidates the physiological and molecular mechanisms of exogenous SA in enhancing plant salt tolerance and provides a practical basis for crop improvement in saline environments.…”
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Differential Regulation of NK Cell Receptors in Acute Lymphoblastic Leukemia by Le Jie Lee, Norfarazieda Hassan, Siti Zuleha Idris, Suresh Kumar Subbiah, Heng Fong Seow, Norhafizah Mohtaruddin, Kian Meng Chang, Raudhawati Osman, Hishamshah Mohd Ibrahim, Sheila Nathan, Maha Abdullah

“…Targeted next-generation sequencing was conducted on 62 activating/inhibitory receptors, ligands, effector, and exhaustion molecules on T-ALL (6 males) and normal controls (NC) (4 males and 4 females). Quantitative PCR (q-PCR) further investigated copy number variation (CNV), methylation index (MI), and mRNA expression of significant genes in T-ALL (14 males), NC (12 males and 12 females), and B-ALL samples (N=12 males and 12 females). …”
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Effects of <i>Bifidobacterium longum longum</i> 35624® on the Symptoms and Quality of Life in Patients with Irritable Bowel Syndrome: Results of the Multicenter Observational Progr... by V. T. Ivashkin, E. A. Poluektova, Z. A. Mamieva

“…The Irritable Bowel Syndrome Quality of Life (IBS-QоL) questionnaire was used to assess the quality of life. …”
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Biochemical analysis of miR-217 and miR-532 in patients with fibromyalgia by Shymaa E. Ayoub, Azza M. Ahmed, Mostafa Y. Abdelwahed, Abeer A. Khalefa, Aeshah A. Awaji, Samer S. Zekry, Enas G. Ibrahim, Hana M. Eid, Shimaa M. Elasmer, Reham Fares

“…The miR-217 and miR-532 serum expressions were detected using quantitative real-time PCR (qRT-PCR). According to our data, the fold changes of miR-217 and miR-532 in fibromyalgia patients were significantly lower than in controls, for miR-217 (median = 0.1359, IQR: 0.038–0.287, P < 0.001) and miR-532 [median = 0.2199, IQR: (0.114–0.421), P < 0.001]. …”
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Spatiotemporal Response of MODIS Derived Vegetation index to climatic condition Case study: Kohgiloyeh O Boirahmad Province of Iran by Mohammad Hossein Nasserzadeh, zahra Hejazizadeh, Zahra Gholampour, Bohloul Alijani

“…So that the in this paer we use MOD13Q1  of MODIS product as NDVI layer for study area. …”
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IGF2BP2 Regulates the Progression of Alzheimer's Disease Through m6A‐Mediated NLRP3 Inflammasome by Wu Jingrui, Yang Haihui, Yan Jinjin, Fang Le

“…Materials & Methods The mRNA and protein expression level were determined using RT‐qPCR and western blot, respectively. MTT assay was carried out to evaluate cell viability. …”
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Ameliorative Effect of Hovenia dulcis Fruit Pedicel Polysaccharides on Alcohol-Induced Intestinal Injury in Mice Investigated Using 16S rRNA Sequencing and Metabolomics by ZHANG Yuchao, ZHU Sijie, LIU Liangyu, WANG Jianyun, ZHANG Jiaxin, SU Zexiong, CHEN Jiangyan, LIU Xudong

“…Additionally, enzyme-linked immunosorbent assay (ELISA), immunohistochemistry, real-time quantitative polymerase chain reaction (qPCR), and Western blot were employed to detect the changes in the expression of intestinal inflammatory factors, tight junction proteins, bile acid metabolism-related genes and proteins. …”
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Comparative Effects of Crocin and Losartan on RAGE, TGF‐β, TNF‐α Gene Expression and Histopathological Changes of the Liver Tissue in Rats With Diabetes by Shahnaz Rajabi, Yaser Mohammadi, Hamid Kabiri‐rad, Mahdiyeh Rajabi‐moghaddam, Azam Rezaei Farimani

“…Serum glucose, ALT and AST levels were measured 4 weeks later. qPCR was used to examine the TNF‐α, TGF‐β and RAGE gene expression in liver tissue. …”
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Upregulated miR-206 Aggravates Deep Vein Thrombosis by Regulating GJA1-Mediated Autophagy of Endothelial Progenitor Cells by Yan Li, Jingping Ge, Yuanyuan Yin, Ruowen Yang, Jie Kong, Jianping Gu

“…The levels of miR-206 and gap junction protein alpha 1 (GJA1) in EPCs and vascular tissues of DVT mice were detected by reverse transcription-quantitative polymerase chain reaction (RT-qPCR). The proliferation, migration, apoptosis, and angiogenesis were tested by cell counting kit-8 (CCK-8) assay, Transwell assay, flow cytometry analysis, and in vitro tube formation assay. …”
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Identification of the Key Genes and Pathways in Esophageal Carcinoma by Peng Su, Shiwang Wen, Yuefeng Zhang, Yong Li, Yanzhao Xu, Yonggang Zhu, Huilai Lv, Fan Zhang, Mingbo Wang, Ziqiang Tian

“…Quantitative real-time polymerase chain reaction (qRT-PCR) was used to verify the expression level of DEGs in EC. …”
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