Identification of ubiquitination-related key biomarkers and immune infiltration in Crohn’s disease by bioinformatics analysis and machine learning by Wei Chen, Zeyan Xu, Haitao Sun, Wen Feng, Zhenhua Huang

“…The efficacy of key genes was validated using ROC curves in an external dataset, and their expression was confirmed in LPS-induced Caco-2 cells through RT-qPCR. A total of 32 ubiquitination-related DEGs were identified, and two key genes (UBE2R2, NEDD4L) were selected. …”
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Analysis of Unsteady Flow Characteristics of Centrifugal Pump under Part Load Based on DDES Turbulence Model by Tang Xin, Liu Zhili, Zhao Meng, Yang Haotian, Jiang Wei, Wang Yuchuan, Chen Diyi

“…The vortex structure and pressure fluctuation of pump internal flow field under part-load condition of Q = 0.4 Qdes are mainly analyzed. The analysis results show that the stall vortex is formed at the inlet of the impeller and evolves to the outlet of the impeller, the front cover to the rear cover according to the fluid flow direction, and then disappears. …”
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Expression of some circulating microRNAs as predictive biomarkers for prognosis and treatment response in glioblastoma by Elham Ali, Marwa Adel Ahmed, May A. Shawki, Lobna R. Ezz El Arab, Mohamed K. Khalifa, Menha Swellam

“…The expression levels of target miRs were analyzed pre- and post-treatment using qRT-PCR and evaluated in relation to both clinical GBM criteria and the patient’s survival modes. …”
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CAFs-released exosomal CREB1 promotes cell progression and immune evasion in thyroid cancer via the positive regulation of CCL20 by Chen Zheng, Hu Hei, Yifei Zhai, Wenbo Gong, Runfang Zhang, Songtao Zhang

“…Currently, we aimed to explore the molecular mechanism of exosome-associated CREB1 and C-C motif chemokine ligand 20 (CCL20) in TC.Methods The mRNA and protein levels were examined via RT-qPCR and western blot. Gene interaction was analyzed using ChIP and dual-luciferase reporter assays. …”
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Sarco(endo)plasmic Reticulum Ca2+-ATPase-2 Gene: Structure and Transcriptional Regulation of the Human Gene by Angel Zarain-Herzberg, Georgina Alvarez-Fernandez

“…The hSERCA2 gene is located in chromosome 12 position q24.1 in Contig NT_009770.8, spans 70 kb, and is organized in 21 exons intervened by 20 introns. …”
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Effects of Insulin-like Growth Factor 1 on the Maintenance of Cell Viability and Osteogenic Differentiation of Gingiva-Derived Mesenchymal Stem Cell Spheroids by Somyeong Hwa, Hyun-Jin Lee, Youngkyung Ko, Jun-Beom Park

“…Additionally, quantitative polymerase chain reaction (qPCR) analysis was performed to determine the expression of RUNX2 and COL1A1. …”
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Deguelin inhibits the proliferation of human multiple myeloma cells by inducing apoptosis and G2/M cell cycle arrest: Involvement of Akt and p38 MAPK signalling pathway by Sun Kening, Chen Ping, Zhang Liang, Lu Zhidong, Jin Qunhua

“…Multiple myeloma cell cycle analysis was performed through flow cytometry. mRNA expression levels were depicted using qRT-PCR. Migration and invasion of multiple myeloma cells were determined with the wound-healing and transwell assays, respectively. …”
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Therapeutic Effect of Gypenosides on Antioxidant Stress Injury in Orbital Fibroblasts of Graves’ Orbitopathy by Chao Ma, Haoyu Li, Wei Liu, Shuwen Lu, Xian Li, Jinyuan Chen, Kaijun Li, Wenzhan Wang

“…The expression of apoptosis-related protein mRNA was analyzed by RT-qPCR technique. ROS and SOD test suites were employed to detect the oxidative stress level in OFs. …”
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Effects of Cholecalciferol on Key Components of Vitamin D-Endo/Para/Autocrine System in Experimental Type 1 Diabetes by Anna Mazanova, Ihor Shymanskyi, Olha Lisakovska, Lala Hajiyeva, Yulia Komisarenko, Mykola Veliky

“…CYP27A1, CYP2R1, CYP27B1, and VDR were assayed by RT-qPCR and Western blotting or visualized by immunofluorescence staining. …”
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Interdigital and Plantar Foot Infections: A Retrospective Analysis of Molecularly Diagnosed Specimens in the United States and a Literature Review by Aditya K. Gupta, Tong Wang, Sara A. Lincoln, Wayne L. Bakotic

“…Here, we examined records of 14,429 skin specimens taken from the feet of dermatology and podiatry outpatients over a 4.6-year period; all specimens were subjected to multiplex qPCR diagnosis for the detection of dermatophytes, <i>Candida</i>, <i>Corynebacterium minutissimum</i>, <i>Pseudomonas</i> and <i>Staphylococcus aureus</i>. …”
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The efficiency of interferon stimulated gene 15 expression in peripheral blood for early pregnancy prediction in Awassi ewes by Eman H. Lazim, Khawla A. Hussein, Dhafer M. Aziz

“…Blood samples were collected on 0, 6, 15, and 25 days of gestation to measure ISG15 expression levels using quantitative real-time PCR (qRT-PCR). The results of the relative expression values of ISG15 in pregnant and non-pregnant ewes on 0, 6, 15, and 25 days of pregnancy were 0.044&plusmn;0.012 vs. 0.049&plusmn;0.009, 0.203&plusmn;0.083 vs. 0.201&plusmn;0.093, 1.839&plusmn;0.594 vs. 0.292&plusmn;0.136, and 0.583&plusmn;0.067 vs 0.200&plusmn;0.051, respectively. …”
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MUSEQuBES: Unveiling Cosmic Web Filaments at z ≈ 3.6 through Dual Absorption and Emission Line Analysis by Eshita Banerjee, Sowgat Muzahid, Joop Schaye, Sebastiano Cantalupo, Sean D. Johnson

“…Here, we report the discovery of an extremely metal-poor ([ X / H ] ≈ −3.7), low-density ( ${\mathrm{log}}_{10}{n}_{{\rm{H}}}/{{\rm{cm}}}^{-3}\,\approx $ −4, corresponding to an overdensity of ≈5) partial Lyman limit system (pLLS) at z  ≈ 3.577 along the quasar sightline Q1317–0507, probing cosmic filaments. Additionally, two other low-metallicity ([ X / H ] ≲ −2) absorption systems are detected at similar redshifts, one of which is also a pLLS. …”
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Senescence-related circRNA circHIF-1α is associated with pancreatic cancer progression by Hao Hua, Yazu Deng, Dijie Zheng, Changhao Wu, Liwen Chen, Binbin Shi, Zhiwei He, Chao Yu

“…Meanwhile, significantly upregulated expression of circHIF-1α in pancreatic cancer tissue detected by reverse transcription-polymerase chain reaction (RT-qPCR) and in situ hybridization (ISH). High circHIF-1α expression levels were found to independently predict poor survival outcomes. …”
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Key wound healing genes as diagnostic biomarkers and therapeutic targets in uterine corpus endometrial carcinoma: an integrated in silico and in vitro study by Fuchuan Jiang, Sajjad Ahmad, Sadia kanwal, Yasir Hameed, Qian Tang

“…These genes exhibited significantly lower expression in UCEC cell lines compared to normal controls, as confirmed by RT-qPCR. Receiver Operating Characteristic (ROC) analysis demonstrated their potential as diagnostic biomarkers, with Area Under the Curve (AUC) values ranging from 0.94 to 1.00. …”
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Study on Mechanical Properties of Gravelly Sand under Different Stress Paths by Dongjie Zhang, Fei Luo, Zhanyuan Zhu, Jiaming Liu, Jing Li, Bailin Li, Tianlang Xue

“…Subsequently, the relationship expression of stress ratio η (q/p) and void ratio e was established, and the trend of void ratio e with the stress path was studied. …”
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Utility of a New Artificial Dermis as a Successful Tool in Face and Scalp Reconstruction for Skin Cancer: Analysis of the Efficacy, Safety, and Aesthetic Outcomes by Fedele Lembo, Liberato Roberto Cecchino, Domenico Parisi, Aurelio Portincasa

“…On follow-up (6 months), tumor recurrences, quality of scars (using the Vancouver Scar Scale), and patient reported outcomes (using FACE-Q Skin Cancer Module) were evaluated. 10 were males and 6 females, with a mean age of 73 years (61–89). …”
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Endoplasmic reticulum stress-related super enhancer promotes epithelial-mesenchymal transformation in hepatocellular carcinoma through CREB5 mediated activation of TNC by Anqi Wang, Sitong Yan, Jiatao Liu, Xiang Chen, Mengyao Hu, Xiao Du, Weijia Jiang, Zhipeng Pan, Lulu Fan, Guoping Sun

“…In this study, we identified 17 ERS-related SEs by comparing ERS-HCC cells with untreated control cells using ChIP-seq and RNA-seq. CRISPR-Cas9 and RT-qPCR identified CAMP responsive element binding protein 5 (CREB5) as a key target of ERS-related SE. …”
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Brain local structural connectomes and the subtypes of the medial temporal lobe parcellations by Zhensheng Li, Jie Ma, Hongmin Bai, Bingmei Deng, Jian Lin, Weimin Wang

“…ObjectiveTo investigate the quantitative characteristics and major subtypes of local structural connectomes for medial temporal lobe (MTL) parcellations.MethodsThe Q-Space Diffeomorphic Reconstruction (QSDR) method was used to track white matter fibers for the ROIs within MTL based on the integrating high-resolution T1 structural MR imaging and diffusion MR imaging of 100 adult Chinese individuals. …”
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Short-Chain Fatty Acids Promote Immunotherapy by Modulating Immune Regulatory Property in B Cells by Cai-Jie Zhou, Bai-Ling Xie, Hai-Yang Han, Yin Wang, Yong-Hua Wang, Jing-Yi Hong, Yi-Xia Wei, Zhi-Gang Liu, Yan Feng, Gui Yang, Ping-Chang Yang

“…The stabilization of IL-10 mRNA in B cells was tested with RT-qPCR. An AR mouse model was developed to test the role of PA in stabilizing the IL-10 expression in B cells. …”
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Metabolics-Based Study on the Therapeutic Mechanism Behind the Effect of Shenhuang Plaster Applied to the Shenque Acupoint on Gastrointestinal Motility in POI Mice by Yanan Shi, Chenglei Wu, Ting Liu, Rongyun Wang, Bin Ding, Qiuhua Sun

“…We employed several behavioral tests and gastrointestinal transit function measurements and performed qRT-PCR analysis, 16S rRNA gene sequencing, and metabolomics analysis on serum metabolites. …”
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