Mechanical Stress-Induced IGF-1 Facilitates col-I and col-III Synthesis via the IGF-1R/AKT/mTORC1 Signaling Pathway by Bin Yan, Canjun Zeng, Yuhui Chen, Minjun Huang, Na Yao, Jie Zhang, Bo Yan, Jiajun Tang, Liang Wang, Zhongmin Zhang

“…IGF-1, collagen I (col-I), and collagen III (col-III) protein and mRNA levels were then detected via ELISA and qPCR, respectively. Moreover, the activation of pIGF-1R, pAKT, and pS6 was examined by Western blot analysis. …”
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Comparison of Conservation Strategies for California Channel Island Oak (Quercus tomentella) Using Climate Suitability Predicted From Genomic Data by Alayna Mead, Sorel Fitz‐Gibbon, John Knapp, Victoria L. Sork

“…We performed whole‐genome sequencing on Island Oak individuals and Q. chrysolepis, a closely related species that hybridizes with Island Oak (127 total), to characterize genetic structure and introgression across its range and assess the relationship between genomic variation and climate. …”
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Tools used to measure quality of life in adults with cystic fibrosis– a systematic review by Dorota Snop-Perkowska, Jakub Świtalski, Katarzyna Wnuk, Paweł Olszewski, Anna Augustynowicz

“…In turn, new tools that may be used in economic analyses (CFQ-R-8 dimensions) or in individualized assessment of quality of life using a mobile application (Q-Life) seem promising. However, further research on large patient populations is necessary to analyze the measurement properties of all tools.…”
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5-Azacytidine Promotes an Inhibitory T-Cell Phenotype and Impairs Immune Mediated Antileukemic Activity by Thomas Stübig, Anita Badbaran, Tim Luetkens, York Hildebrandt, Djordje Atanackovic, Thomas M. C. Binder, Boris Fehse, Nicolaus Kröger

“…T-cells from healthy donors were treated with 5-Aza and analyzed by qRT-PCR and flow cytometry for changes in gene expression and phenotype. …”
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Foxp3 Expression in Liver Correlates with the Degree but Not the Cause of Inflammation by Matthaios Speletas, Nikoletta Argentou, Georgios Germanidis, Themistoclis Vasiliadis, Konstantinos Mantzoukis, Kalliopi Patsiaoura, Pavlos Nikolaidis, Vaios Karanikas, Konstantinos Ritis, Anastasios E. Germenis

“…Liver biopsies obtained from 69 individuals (26 chronic HBV hepatitis, 14 chronic HCV hepatitis, 11 nonalcoholic fatty liver disease, 8 autoimmune diseases, 2 methotrexate-related toxicity, and 8 controls) were examined, by qRT-PCR, for the mRNA expression of Foxp3, IL-10, TGF-β1, Fas, FasL, TRAIL, caspase-3, TNF-α, IFN-γ, and IL-1β. …”
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The immunity-promoting activity of porcine placenta in mice as an immunomodulator for functional foods by Zhiwei Zhou, Dan Wang, Wei Liu, Lang He, Pengkuan Liang, Junli Hao, Qun Sun

“…Mice model experiment revealed that compared with the control, the PPH treatment significantly improved the spleen index (P < 0.05) by increasing the phagocytic rate of macrophages from 20 % to 60 % and the conversion rate of T lymphocytes from 8 % to 60 %. The qPCR analysis disclosed that the porcine placenta powder enhanced mice immunity via promoting the expression of Th1 cytokines of interleukin-2 (IL-2) and IFN-γ, especially the former, by almost 8 times in the spleens of male mice, while inhibited Th2 cytokines of IL-4 and IL-10. …”
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Knockdown of YAP/TAZ Inhibits the Migration and Invasion of Fibroblast Synovial Cells in Rheumatoid Arthritis by Regulating Autophagy by Wei Zhou, Qin Shen, Hui Wang, Jie Yang, Chen Zhang, Zijing Deng, Keyan Wu, Yang Zhou, Jing Zeng, Yu Zhang, Weigan Shen

“…Reverse transcription quantitative real-time polymerase chain reaction (RT-qPCR) and western blotting assays were performed to examine the expression of indicated genes. …”
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A Quantum-Classical Collaborative Training Architecture Based on Quantum State Fidelity by Ryan L'Abbate, Anthony D'Onofrio, Samuel Stein, Samuel Yen-Chi Chen, Ang Li, Pin-Yu Chen, Juntao Chen, Ying Mao

“…On the quantum side, we propose a quantum-state-fidelity-based evaluation function to iteratively train the network through a feedback loop between the two sides. co-TenQu has been implemented and evaluated with both simulators and the IBM-Q platform. Compared to state-of-the-art approaches, co-TenQu enhances a classical deep neural network by up to 41.72&#x0025; in a fair setting. …”
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BRAF regulates circPSD3/miR-526b/RAP2A axis to hinder papillary thyroid carcinoma progression by Chuang Li, Xiaojuan Zhao, Jingge Zhao, Jing Zhao, Lemei An, Gang Wu

“…This study aims to probe the biofunction and molecular mechanism of circPSD3 in PTC pathogenesis. Methods RT-qPCR was utilized to measure the expression of circPSD3 and BRAF in PTC tissues and cells. …”
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Development of a Portable Rapid Detection Method for Porcine Epidemic Diarrhea Virus Using Reverse Transcription-Recombinase-Aided Amplification Technology by Yiran Zhao, Weijie Yi, Qicheng Yang, Jiahao Li, Yanke Shan, Fei Liu

“…When applied to clinical samples, the results were 100% consistent with those obtained by RT-qPCR. This method is distinguished by its portable instrumentation and simple operation, making it particularly suitable for resource-constrained settings.…”
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Type 2 Diabetic Mellitus Inhibits Skin Renewal through Inhibiting WNT-Dependent Lgr5+ Hair Follicle Stem Cell Activation in C57BL/6 Mice by Minghui Wang, Shangsheng Yao, Dehua He, Mulan Qahar, Jinqing He, Meifang Yin, Jun Wu, Guang Yang

“…The altered genes were screened by RNA sequencing and verified by qRT-PCR. In addition, Lgr5 + GFP/mTmG transgenic mice were used to observe the effect of T2DM on Lgr5 hair follicle stem cells (HFSC). …”
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Peripheral Blood-Derived Mesenchymal Stem Cells Modulate Macrophage Plasticity through the IL-10/STAT3 Pathway by Qi-Ming Pang, Rui Yang, Meng Zhang, Wang-Hui Zou, Nan-Nan Qian, Qi-Jing Xu, Hui Chen, Jia-Chen Peng, Xiao-Ping Luo, Qian Zhang, Tao Zhang

“…In this study, a Transwell system was used for coculturing macrophages and PBMSCs. ELISA and RT-qPCR analysis found that PBMSCs and their conditioned media (P-CM) significantly induced the expression of IL-10, while significantly inhibiting the expression of IL-1β and TNF-α; moreover, this effect could be reversed by adding Ab9969 (an IL-10 neutralizing antibody) and Stattic (a STAT3 inhibitor). …”
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ТЕОРЕТИКО-ПРАВОВОЕ ИЗМЕРЕНИЕ МИГРАЦИОННОГО ПРОЦЕССА МАТЕРИНСКОЙ МИГРАЦИИ by Г.Г. Турысбекова, И.М. Искаков

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Ameliorative Impact of Liraglutide on Chronic Intermittent Hypoxia-Induced Atrial Remodeling by Jun Wang, Yongzheng Liu, Changhui Ma, Yue Zhang, Meng Yuan, Guangping Li

“…Immunohistochemical staining, RT-qPCR, and Western blotting were performed to evaluate the marker levels of AAR and AER and the expression of genes and proteins of the miR-21/PTEN/PI3K/AKT signaling pathway, respectively. …”
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Elevated and Correlated Expressions of miR-24, miR-30d, miR-146a, and SFRP-4 in Human Abdominal Adipose Tissue Play a Role in Adiposity and Insulin Resistance by Yury O. Nunez Lopez, Gabriella Garufi, Magdalena Pasarica, Attila A. Seyhan

“…Abdominal adipose tissue (AbdAT) levels of thirteen microRNAs (miRNAs), SFRP4, and VEGF in lean nondiabetic subjects (n=7), subjects with obesity (n=5), and subjects with obesity and type 2 diabetes (T2DM) (n=5) were measured by qPCR. Insulin sensitivity was measured by the euglycemic-hyperinsulinemic clamp. …”
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Limited transmission of avian influenza viruses, avulaviruses, coronaviruses and Chlamydia sp. at the interface between wild birds and a free-range duck farm by Chloé Le Gall-Ladevèze, Benjamin Vollot, Julien Hirschinger, Laëtitia Lèbre, Rachid Aaziz, Karine Laroucau, Jean-Luc Guérin, Mathilde Paul, Julien Cappelle, Guillaume Le Loc’h

“…The presence of the four pathogens was tested by q(RT-)PCR, and the immunity of wild birds to AIV and Newcastle disease virus (NDV) was tested by ELISA. …”
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Metformin and Probiotics Interplay in Amelioration of Ethanol-Induced Oxidative Stress and Inflammatory Response in an In Vitro and In Vivo Model of Hepatic Injury by Farhin Patel, Kirti Parwani, Dhara Patel, Palash Mandal

“…Endoplasmic reticulum (ER) stress markers, inflammatory markers, lipid metabolism, reactive oxygen species (ROS) production, and oxidative stress were evaluated, using qRT-PCR, Oil red O staining, fluorimetry, and HPLC. …”
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Integration of digital technologies to improve the efficiency of small and medium-sized agricultural enterprises by Zhanna Yermekova, Svetlana Romanenko, Gaukhar Zhanibekova, Bakytzhamal Aitzhanova, Nurlan Apakhayev

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Music Activities for Language Learning in the Spanish Classroom by Manuel Vidal, Christine Ericsdotter Nordgren

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Elucidating the molecular basis of salt tolerance in potatoes through miRNA expression and phenotypic analysis by Caicai Lin, Shuangshuang Zheng, Kui Liu, Ru Yu, Peiyan Guan, Baigeng Hu, Lingling Jiang, Mengyu Su, Guodong Hu, Qingshuai Chen, Xia Zhang

“…Transcriptome analysis of the highly tolerant variety Z1076-1 identified 68 differentially expressed miRNAs (DE miRNAs). qRT-PCR validation for eight randomly selected DE miRNAs confirmed consistent expression trends with transcriptome data. …”
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