Glucose-Sensing Carbohydrate Response Element-Binding Protein in the Pathogenesis of Diabetic Retinopathy by Christopher R. Starr, Assylbek Zhylkibayev, Oleg Gorbatyuk, Alli M. Nuotio-Antar, James Mobley, Maria B. Grant, Marina Gorbatyuk

“…We used diabetic human and mouse retinal cryosections analyzed by immunohistochemistry. qRT-PCR was performed to quantify gene expression. …”
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Valproic Acid Enhances Venetoclax Efficacy in Targeting Acute Myeloid Leukemia by Renshi Kawakatsu, Kenjiro Tadagaki, Kenta Yamasaki, Yasumichi Kuwahara, Tatsushi Yoshida

“…The expression of pro-apoptotic proteins Bax and Bak was measured by RT-qPCR. Results: Venetoclax and VPA individually had only mild effects on AML cell proliferation. …”
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Enhancement of Growth, Antioxidant Activity, and Immunity in Nile Tilapia (<i>Oreochromis niloticus</i>) Through Recombinant <i>Bacillus subtilis</i> Expressing L-Gulonolactone Oxi... by Jirawadee Kaewda, Surintorn Boonanuntanasarn, Papungkorn Sangsawad, Pimpisut Manassila, Chatsirin Nakharuthai

“…The VC, BS, and BS+GULO groups exhibited increased total immunoglobulin and lysozyme activity; however, only the VC and BS+GULO groups showed elevated alternative complement 50 levels, phagocytic activity and improved antioxidant parameters compared to the control. HPLC and qRT-PCR analyses revealed elevated serum vitamin C and intestinal <i>GULO</i> mRNA levels in the BS+GULO group. …”
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Identification and functional validation of a new gene conferring resistance to Soybean Mosaic Virus strains SC4 and SC20 in soybean by Muhammad Muzzafar Raza, Huiying Jia, Muhammad Khuram Razzaq, Bowen Li, Kai Li, Junyi Gai

“…The results were validated using qRT-PCR, virus induced gene silencing (VIGS), and gene-sequence. …”
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Intra-tumoral sphingobacterium multivorum promotes triple-negative breast cancer progression by suppressing tumor immunosurveillance by Zhikai Mai, Liwu Fu, Jiyan Su, Kenneth K.W. To, Chuansheng Yang, Chenglai Xia

“…Differential genes of tumor cells stimulated by bacteria were analyzed by transcriptomics and validated by qPCR assay. Results We found that Sphingobacterium displayed high abundance in cancerous tissues. …”
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Role of the Btk-PLCγ2 Signaling Pathway in the Bone Destruction of Apical Periodontitis by Lina Wang, Hong Zhang, Ming Dong, Meina Zuo, Shuo Liu, Ying Lu, Weidong Niu

“…By silencing Btk, the expression of Btk, PLCγ2, and NFATc-1 was detected by real-time qPCR and Western blot, and osteoclastogenesis was detected by enzyme histochemical staining to further confirm the role of Btk in bone resorption. …”
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Biological effectiveness of combined exposure to neutrons and gamma radiation applied in two orders of sequence: Relevance for biological dosimetry after nuclear emergencies by Tomas Palmqvist, Milagrosa Lopez-Riego, Martin Bucher, Ursula Oestreicher, Stefan Pojtinger, Ulrich Giesen, Iuliana Toma-Dasu, Andrzej Wojcik

“…Dicentric chromosomes were analyzed by light microscopy and the expression of six known radiation-responsive genes BBC3, CDKN1A, FDXR, GADD45A, MDM2, and XPC were analyzed by RT-qPCR. Results: Per unit dose, exposure to neutrons lead to a higher level of dicentrics and gene expression as compared to gamma radiation. …”
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Atractylenolide-I prevents abdominal aortic aneurysm formation through inhibiting inflammation by Shuxiao Chen, Xiaotian Liu, Xincheng Zhou, Weixiao Lin, Minting Liu, Haoran Ma, Keli Zhong, Qiming Ma, Chengjian Qin, Chengjian Qin

“…The pathological and molecular alteration was investigated by immunostaining, ELISA, qRT-PCR and Western blotting.ResultsATL-I inhibited the dilatation of the abdominal aorta and decreased the incidence of AAA. …”
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Gestation Related Gene Expression of the Endocannabinoid Pathway in Rat Placenta by Kanchan Vaswani, Hsiu-Wen Chan, Hassendrini N. Peiris, Marloes Dekker Nitert, Ryan J. Wood Bradley, James A. Armitage, Gregory E. Rice, Murray D. Mitchell

“…Biological replication of the results was performed by qPCR (cohort 2). Four genes showed differential expression (Mgll, Plcd4, Ptgs2, and Pld1), from mid to late gestation. …”
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Genome-wide investigation of ABCB, PIN, and AUX/LAX gene families and their involvement in the formation of leaf protrusions in Sesamum indicum by Yanli Li, Yujia Ma, Huihui Gong, Xinxiao Cui, Xin Wang, Yuling Dong, Ying Chen, Junsheng Zhao

“…Transcriptome screening and RT-qPCR analysis revealed that SiABCB3, SiABCB6, and SiPIN10 positively regulate PAT, whereas SiABCB7 and SiABCB9 negatively regulate PAT in G1358. …”
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Comprehensive Analysis of Transcriptome Sequencing Data in the Lung Tissues of COPD Subjects by Woo Jin Kim, Jae Hyun Lim, Jae Seung Lee, Sang-Do Lee, Ju Han Kim, Yeon-Mok Oh

“…A total of 2312 genes were differentially expressed between the lung tissues of COPD and control subjects (false discovery rate corrected q<0.01). The expression of genes related to oxidative phosphorylation and protein catabolism was reduced and genes related to chromatin modification were dysregulated in lung tissues of COPD subjects. …”
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Study on the Effect of CeO₂ on the Performance of WC + Ni60 Laser Cladding Coating by Jingquan Wu, Jianwen Zhang, Dianlong Chen, Jiang Huang, Wenqing Shi, Fenju An, Xianglin Wu

“…Various cladding layers of WC + Ni60, incorporating differing mass fractions of CeO₂, were created on the surface of Q235 steel utilizing laser cladding technology. …”
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A hyperspectral and multi-angular synthetic dataset for algorithm development in waters of varying trophic levels and optical complexity by J. Pitarch, V. E. Brando, V. E. Brando

“…Derived AOPs encompass an array of above- and below-surface reflectances, diffuse attenuation coefficients, average cosines, and <span class="inline-formula"><i>Q</i></span> factor. The dataset is distributed in 5000 files, each encapsulating a specific IOP scenario, ensuring sufficient data volume for each represented water type. …”
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The Value of Immune-Related Genes Signature in Osteosarcoma Based on Weighted Gene Co-expression Network Analysis by Xin Wang, Li Gan, Ju Ye, Mengjie Tang, Wei Liu

“…The results of immunohistochemical staining and qPCR showed that EVI2B was a tumor suppressor gene. …”
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Transforming monosaccharides: Recent advances in rare sugar production and future exploration by Shin-ichi Nakakita, Jun Hirabayashi

“…In this minireview, we adopt a hypothesis on the origin and evolution of elementary hexoses, previously presented by one of the authors (Hirabayashi, Q Rev Biol, 1996, 71:365–380). In this scenario, monosaccharides, which constitute various kinds of glycans in nature, are assumed to have been generated by formose reactions on the prebiotic Earth (chemical evolution era). …”
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Grem1 inhibits osteogenic differentiation of MBMSCs in OVX rats through BMP/Smad1/5 signaling pathway by Shan-shan Yang, Min LinHu, Xiao-hua Hu, Si-si Jiang, Wen-yue Hu, Xiao-hong Yang

“…Techniques such as Western Blot (WB) and Real-Time Quantitative PCR (RT-qPCR) were employed to determine changes in Grem1 expression in MBMSCs of postmenopausal rats and its effect on osteogenic differentiation. …”
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Activation of FGD5-AS1 Promotes Progression of Cervical Cancer through Regulating BST2 to Inhibit Macrophage M1 Polarization by Guokun Liu, Xuan Du, Li Xiao, Qing Zeng, QianLing Liu

“…The level of FGD5-AS1 and BST2 was detected by qRT-PCR in cervical cancer cells. FGD5-AS1 and BST2 expression was significantly upregulated in cervical cancer cells. …”
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Occurrence of antibiotics and antibiotic resistance genes at various stages of different aquaculture modes surrounding Tai Lake, China by Congcong Wu, Congcong Wu, Haitao Ye, Mingzhu Xu, Xuan Zhao, Xuejie Zhao, Lina Li, Mingzhi Li, Yanfei Wei, Yuru Li, Baolan Hu, Baolan Hu

“…However, the occurrence of antibiotics and ARGs under different modes and stages of aquaculture has rarely been systematically studied.MethodsThis paper uses qPCR, LC-MS, and High-Throughput sequencing across different culture modes and stages to investigate antibiotics, resistance genes, and microbial communities in the water bodies, and analyze contamination differences between these modes.ResultsThe quinolone and chloramphenicol were the main antibiotics, and the highest absolute abundance genes were quinolone resistance genes (qnrB) and quinolone resistance genes (sul1), with the mobile genetic element (MGE) intI1, both of which exhibited a gradual seasonal increase. …”
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Orthodontic Treatment and Maxillary Anterior Segmental Distraction Osteogenesis of a Subject with Williams–Beuren Syndrome and Isolated Cleft Palate: A Long-Term Follow-Up from the... by Tetsutaro Yamaguchi, Tatsuo Shirota, Mohamed Adel, Masahiro Takahashi, Shugo Haga, Ryo Nagahama, Misato Nakashima, Mayu Furuhata, Takaaki Kamatani, Koutaro Maki

“…Williams–Beuren syndrome (WBS) is a rare multisystem disorder caused by a hemizygous deletion of the elastin gene on chromosome 7q11.23. WBS patients have characteristic skeletal features and dental anomalies accompanied by mental retardation, a friendly outgoing personality, and mild to moderate intellectual disability or learning problems. …”
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Machine learning potential predictor of idiopathic pulmonary fibrosis by Chenchun Ding, Quan Liao, Renjie Zuo, Shichao Zhang, Zhenzhen Guo, Junjie He, Ziwei Ye, Weibin Chen, Sunkui Ke

“…Finally, the roles of PODNL1 and PIGA as biomarkers were validated through in vivo and in vitro experiments using qRT-PCR, Western blotting, and immunohistochemistry.ResultsThese findings suggest that PODNL1 and PIGA may serve as critical biomarkers for IPF onset and contribute to its pathogenesis.DiscussionThis study highlights their potential for early biomarker discovery and risk prediction in IPF, offering insights into disease mechanisms and diagnostic strategies.…”
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