Postnatal cytomegalovirus infection and its effect on hearing and neurodevelopmental outcomes among infants aged 3-10 months: A cohort study in Eastern Uganda. by Noela Regina Akwi Okalany, David Mukunya, Peter Olupot-Olupot, Martin Chebet, Francis Okello, Andrew D Weeks, Fred Bisso, Thorkild Tylleskär, Kathy Burgoine, Ingunn Marie Stadskleiv Engebretsen

“…CMV status was determined by PCR screening of saliva samples, with positive results confirmed using urine samples (Chai Open qPCR, Santa Clara, CA). From the established cohort, 424 infants were successfully followed up between 3 to 10 months of age. …”
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Effect of Gegen Qinlian Decoction on Cardiac Gene Expression in Diabetic Mice by Jing Han, Zhenglin Wang, Wei Xing, Yueying Yuan, Yi Zhang, Tiantian Lv, Hongliang Wang, Yonggang Liu, Yan Wu

“…The RNA-seq results were validated by the results of RT-qPCR of 4 genes identified as differentially expressed. …”
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Semaphorin 3A Inhibits Inflammation in Chondrocytes under Excessive Mechanical Stress by Chikako Sumi, Naoto Hirose, Makoto Yanoshita, Mami Takano, Sayuri Nishiyama, Yuki Okamoto, Yuki Asakawa, Kotaro Tanimoto

“…Gene expression of IL-1β, TNF-ɑ, COX-2, MMP-3, and MMP-13 was examined by qPCR analysis. Furthermore, the phosphorylation of AKT, ERK, and NF-κB was detected by Western blot analysis. …”
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Effectiveness Trade-Off Between Green Spaces and Built-Up Land: Evaluating Trade-Off Efficiency and Its Drivers in an Expanding City by Xinyu Dong, Yanmei Ye, Tao Zhou, Dagmar Haase, Angela Lausch

“…Interaction detection demonstrates significant synergistic effects between pairs of drivers, such as the Normalized Difference Vegetation Index and building height, and the number of patches of green spaces and the patch cohesion index of built-up land, with <i>q</i>-values of 0.298 and 0.137, respectively. …”
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Effects of Placenta-Derived Mesenchymal Stem Cells on the Particulate Matter-Induced Damages in Human Middle Ear Epithelial Cells by So Young Kim, Seung Ha Oh, Jun Ho Lee, Myung-Whan Suh, Moo Kyun Park

“…Cells were harvested on days 0, 1, and 4, and the expression of the inflammatory genes TNFα, COX2, IL1β, IL6, and MUC5B in HMEECs and anti-inflammatory genes PTGES, TGFβ, and VEGF in PL-MSCs was examined by qRT-PCR. The culture media were collected to measure the secreted PGE2 level using an enzyme-linked immunosorbent assay. …”
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Effect of miR-223-3p and miR-328a-3p Knockdown on Allergic Airway Inflammation in Rat Precision-Cut Lung Slices by Joanna Nowakowska, Maria Kachel, Wojciech Langwiński, Kamil Ziarniak, Aleksandra Szczepankiewicz

“…RNA was isolated from PCLSs and analyzed by qPCR. We also examined the proteins in the culture medium using the Magnetic Luminex Assay. …”
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Particle motion and QPOs around Euler-Heisenberg black hole immersed in cold dark matter halo by G. Mustafa, S.K. Maurya, Tayyab Naseer, Arzu Cilli, Ertan Güdekli, Assmaa Abd-Elmonem, Neissrien Alhubieshi

“…This black hole solution is characterized by five parameters, namely, the black hole mass M, the feature radius R, the parameter ρ describing the density of the cold dark matter halo, the quantum electrodynamics parameter α, and the black hole charge Q. We confine ourselves to an equatorial plane and examine the stability of equatorial circular orbits with the help of the effective potential. …”
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KLF5 silencing restrains proliferation, invasion, migration and angiogenesis of gallbladder carcinoma cells by transcriptional regulation of PDGFA by Xiaowei Lu, Kui Hu, Dandan Zhang, Xuefeng Yin, Jifeng Nie, Kai Zhao

“…Methods The expressions of KLF5 and platelet-derived growth factor subunit A (PDGFA) in GBC cell lines were analyzed by qRT-PCR and western blot assay. Cell proliferation was assessed utilizing the Cell Counting Kit-8 assay and EDU staining. …”
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Does the Harvesting Site Influence the Osteogenic Potential of Mesenchymal Stem Cells? by Van Thi Nguyen, Irene Tessaro, Antonio Marmotti, Camilla Sirtori, Giuseppe M. Peretti, Laura Mangiavini

“…These findings were confirmed by quantitative polymerase chain reaction (qPCR). In chondrogenic and adipogenic conditions, bone marrow cells possessed a predominant differential capacity compared with the others, illustrated by high collagen type II expression together with a cartilage-like lacuna structure and the presence of fat-specific markers, respectively. …”
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The Molecular Diagnosis Protocols of New Coronavirus (COVID-19); Specificity and Sensitivity an Overview by Abdullah Ahmed Hama, Othman Abdulrahman Mohammed, Fatima Mahmud Ali, Osama Hamid Shareef, Sardar Muhammad Wli, Sabiha Sharif, Syamand Ahmed Qadir

“…Quantitative polymerase chain reaction (qPCR) is the recommended technique used with varied sensitivity due to primers variation and specimen type. …”
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Attitudes to Medication after Kidney Transplantation and Their Association with Medication Adherence and Graft Survival: A 2-Year Follow-Up Study by Mirjam Tielen, Job van Exel, Mirjam Laging, Denise K. Beck, Roshni Khemai, Teun van Gelder, Michiel G. H. Betjes, Willem Weimar, Emma K. Massey

“…Kidney recipients participated in a Q-methodological study 6 weeks after transplantation. …”
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Nanopore adaptive sampling accurately detects nucleotide variants and improves the characterization of large‐scale rearrangement for the diagnosis of cancer predisposition by Sandy Chevrier, Corentin Richard, Marie Mille, Denis Bertrand, Romain Boidot

“…Sequencing was performed on Oxford Nanopore (ONT) R10.4.1 MinION flowcells with the Q20+ chemistry. Results In our cohort, 11 samples had large‐scale rearrangements (LSR), which were all detected with ONT sequencing. …”
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Clinical Evaluation of the Diagnostic Role of MicroRNA-155 in Breast Cancer by Fatemeh Hosseini Mojahed, Amir Hossein Aalami, Vahid Pouresmaeil, Amir Amirabadi, Mahdi Qasemi Rad, Amirhossein Sahebkar

“…The expression of miR-155 was measured using RT-qPCR. Demographic and histochemical data were extracted from patient documents. …”
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Alterations of Dopamine-Related Transcripts in A11 Diencephalospinal Pathways after Spinal Cord Injury by Shunyi Zhao, Jaclyn H. DeFinis, Shaoping Hou

“…Meanwhile, A11 neurons in the brain were explicitly isolated by laser capture microdissection, and single-cell qPCR was employed to evaluate mRNA levels in the soma. …”
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The Association of a Genetic Variant in SCAF8-CNKSR3 with Diabetic Kidney Disease and Diabetic Retinopathy in a Chinese Population by Li Jin, Tao Wang, Song Jiang, Miao Chen, Rong Zhang, Cheng Hu, Weiping Jia, Zhihong Liu

“…Genome-wide association studies found rs955333 located in 6q25.2 was associated with diabetic kidney disease in multiple ethnic populations, including European Americans, African Americans, and Mexican Americans. …”
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Improving the binding affinity of plastic degrading cutinase with polyethylene terephthalate (PET) and polyurethane (PU); an in-silico study by Deves Sabari V L, Gokulnath Rajmohan, Roshine S B, Srivaishnavi S, Kishore Nagasubramanian, Senthil Kumar G, Ponnusami Venkatachalam

“…In-silico Alanine Scanning Mutagenesis (ASM) and Site Saturation Mutagenesis (SSM) were performed as screening tests to find variants of cutinase with better docking scores for both ligands, specifically S136D, N28M, and S136Q. Molecular Dynamic Simulation (MDS) was performed for Wild Type (WT) cutinase, variants, and their respective complexes formed with the ligands. …”
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Epidemiology and transmission patterns of Cryptosporidium spp., and Giardia duodenalis within a One Health framework in rural areas of Eastern Algeria by Sadiya Maxamhud, Nassiba Reghaissia, AbdElKarim Laatamna, Eleni Gentekaki, Anastasios D. Tsaousis

“…Detection of G. duodenalis was performed by qPCR targeting the SSU rRNA gene, followed by amplification of tpi, bg and gdh genes for genotyping and subtyping. …”
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Combined MSC and GLP-1 Therapy Modulates Collagen Remodeling and Apoptosis following Myocardial Infarction by Elizabeth J. Wright, Nigel W. Hodson, Michael J. Sherratt, Moustapha Kassem, Andrew L. Lewis, Christine Wallrapp, Nadim Malik, Cathy M. Holt

“…MSC + GLP-1 conditioned media demonstrated antiapoptotic effects on ischaemic human cardiomyocytes in vitro. In vivo, qRT-PCR revealed large changes in the expression of several genes involved in extracellular matrix remodeling, which were altered following MSC ± GLP treatment. …”
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Identification and functional characterization of the RPP13 gene family in potato (Solanum tuberosum L.) for disease resistance by Baoqi Yuan, Chuang Li, Qingfeng Wang, Qi Yao, Xiaowei Guo, Yuhang Zhang, Zhongwei Wang

“…Expression pattern analysis showed that StRPP13-8, StRPP13-10, and StRPP13-23 are highly expressed in various tissues, indicating their involvement in basic physiological functions, whereas StRPP13-6 and StRPP13-25 are mainly induced under specific pathogen infection conditions. Transcriptome and qRT-PCR analyses further revealed functional divergence of the RPP13 gene family in response to potato scab disease. …”
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Genome wide identification of MATE and ALMT gene family in lentil (Lens culinaris Medikus) and expression profiling under Al stress condition by Ankita Tripathi, Dharmendra Singh, Jyotika Bhati, Deepti Singh, Jyoti Taunk, Jawaher Alkahtani, Abdulrahman Al-Hashimi, Madan Pal Singh

“…Expression analyses of LcMATE and LcALMT genes were performed using quantitative real-time polymerase chain reaction (qRT-PCR). Six genes namely, LcM1, LcM42, LcM46, LcM47, LcALMT8 and LcALMT14 responded to Al stress with varying levels of expression patterns at different time points (3, 6, 12 and 24 h). …”
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