MFG-E8 Exerts Neuroprotection in Neural Stem Cells Induced by Anesthetic Sevoflurane via Regulating the PI3K/AKT Pathways by Minmin Cai, Liufang Sheng

“…Then, the mRNA expression of MFG-E8 was detected by qRT-PCR. The expression of SOD, GSF-Px, and MDA was assessed using ELISA assay. …”
Get full text

Image Comparison on T2 Qtse Lumbal Examination using Grappa Technique with and without Magnetization Transfer Contrast in Degenerative Disc Disease Case by Karina Sandra Arief Apriliani, Sri Andreani Utomo, Muhaimin, Lailatul Muqmiroh

“…Purpose: To compare the quality of image results on lumbar MRI examination of the sagittal T2 qTSE sequence in the case of Degenerative Disc Disease with and without MTC activation. …”
Get full text

Genome-wide identification and expression analysis of GRAS transcription factors under cold stress in diploid and triploid Eucalyptus by Jiannan Liu, Hao Chen, Chenhe Li, Kang Du, Jun Yang

“…Transcriptome data analysis and quantitative real-time PCR (qRT-PCR) experiments in diploid and triploid Eucalyptus urophylla found that some EgrGRAS genes exhibited upregulated expression under different cold treatment durations, with certain genes from the LISCL, PAT1, and DELLA subfamilies significantly upregulated in triploid Eucalyptus. …”
Get full text

Agri-food systems: A regional perspective in innovation capabilities by Monica Gisela Dueñas Gómez, Leidy Dayhana Guarin Manrique, Efrén Romero Riaño, Luis Eduardo Becerra Ardila

“…Las principales recomendaciones se centran en promover la gestión de la integración vertical, horizontal y lateral y la colaboración virtual.Clasificación JEL: O3, Q1…”
Get full text

Comparative Study on Muscle Fiber Types of Longissimus Dorsi of Xinjiang Brown Cattle and Angus Cattle of Different Months by Ya-wei Sun, Miao Qu, Jiayinaguli Tuersunbai, Xuan-yi Wang, Tuolihanayi Tuoliken, Xiao-hong Zhang, Gang Yao, Huijun Shi

“…The morphology of the muscle was compared by ATP enzyme staining and SDH enzyme staining, and its gene expression was detected by qRT-PCR. The mRNA expression levels of Myhc-I in 3-month-old Xinjiang brown cattle were significantly higher than those in Angus cattle of the same age (P<0.01). …”
Get full text

Hepatoma cell-derived exosomal SNORD52 mediates M2 macrophage polarization by activating the JAK2/STAT6 pathway by Yaqiong Zhang, Bo Li, Wanhong Gu, Linna Fan, Xiaofan Wang, Meifen Xu, Minqi Zhu, Chong Jin

“…SNORD52 expression was assessed using qRT-PCR. The levels of JAK2/STAT6 pathway-related proteins were analyzed using western blotting. …”
Get full text

ANGPTL4 Attenuates Ang II-Induced Atrial Fibrillation and Fibrosis in Mice via PPAR Pathway by Xi Zhu, Xiaogang Zhang, Xinpeng Cong, Luoning Zhu, Zhongping Ning

“…The fibrosis was evaluated with Masson’s trichrome staining in the atrial myocardium. mRNA levels of IL-1β, IL-6, collagen I, and collagen III were measured using real-time qRT-PCR. Protein levels of PPARα, PPARγ, CPT-1, and SIRT3 were measured using Western blotting. …”
Get full text

Transcriptomic data reveals MYC as an upstream regulator in laying hen follicular recruitment by Ashley E Kramer, Kathryn M Ellwood, Nicole Guarino, Cong-Jun Li, Aditya Dutta

“…Further validation through qRT-PCR confirmed these findings. Using Qiagen's Ingenuity Pathway Analysis we identified MYC proto-oncogene (C-Myc) as a pivotal upstream regulator, controlling genes essential for cell proliferation and differentiation. …”
Get full text

Health literacy and influencing factors in university students across diverse educational fields in Kazakhstan by Zhaniya Dauletkaliyeva, Zhanerke Bolatova, Nurbek Yerdessov, Karina Nukeshtayeva, Olzhas Zhamantayev, Aliya Takuadina, Gaukhar Kayupova

“…The existing literature from adult populations in Kazakhstan suggests associations between higher HL and socioeconomic and demographic factors. The HLS19-Q12 tool was used in this study to assess the HL level of 3230 students with various backgrounds. …”
Get full text

Innovative strategy for full-scale polar components explicition and ultrasonic-assisted optimization of Astragalus membranaceus flower by Yumei Wang, Meiling Gu, Meng Zhang, Jialin Mao, Yujian Han, Qi Liu

“…In view of this, in order to obtain the overall chemical understanding, we attempted to use Astragalus membranaceus flower, an abundant exploitable resource, to screen a novel extraction mixing scheme via gradient solvents based on the ions’ quantity detected in UHPLC-Q-TOF-MS/MS approach. Samples were firstly extracted by different concentration ethanol, and then, six mixing schemes were investigated and one scheme with maximum detected ions was screened out. …”
Get full text

Differences in gene expression profile between vocal cord Leukoplakia and normal larynx mucosa by gene chip by Jianhua Peng, He Li, Jun Chen, Xianming Wu, Tao Jiang, Xiaoyun Chen

“…Methods Gene Ontology and pathway analyses were performed to determine the significant function and pathways of the differentially expressed mRNAs. qRT-PCR was performed to further validate the expression of selected lncRNAs and mRNAs in vocal cord leukoplakia. …”
Get full text

Circular RNA ITCH Is a Tumor Suppressor in Clear Cell Renal Cell Carcinoma Metastasis through miR-106b-5p/PDCD4 Axis by Ping Gao, Yong Huang, Yanmei Hou, Qian Li, Haimei Wang

“…The expression of circ-ITCH in ccRCC tissues and cells was evaluated utilizing qRT-PCR. Transwell assay and wound healing were applied to investigate migration and invasion of ccRCC cells. …”
Get full text

Robot Dynamic Path Planning Based on Prioritized Experience Replay and LSTM Network by Hongqi Li, Peisi Zhong, Li Liu, Xiao Wang, Mei Liu, Jie Yuan

“…To address the issues of slow convergence speed, poor dynamic adaptability, and path redundancy in the Double Deep Q Network (DDQN) within complex obstacle environments, this paper proposes an enhanced algorithm within the deep reinforcement learning framework. …”
Get full text

Intratumor Genetic Heterogeneity of Breast Carcinomas as Determined by Fine Needle Aspiration and TaqMan Low Density Array by Maria B. Lyng, Anne-Vibeke Lænkholm, Niels Pallisgaard, Werner Vach, Ann Knoop, Martin Bak, Henrik J. Ditzel

“…Non-amplified RNA was investigated by the novel qRT-PCR-based technique, Low Density Array (LDA) using 4 reference genes and 44 target genes. …”
Get full text

HYDRAULIC ELEVATOR INSTALLATION ESTIMATION FOR THE WATER SOURCE WELL SAND-PACK CLEANING UP by V. V. Ivashechkin

“…For determining water flow in the supply and the water-jet pipe lines the author proposes to employ graphical approach allowing finding the regime point in Q–H chart by means of building characteristics of the pump and the pipe-lines. …”
Get full text

Do Salivary <i>Cullin7</i> Gene Expression and Protein Levels Provide Advantages over Plasma Levels in Diagnosing Breast Cancer? by Ceren Tilgen Yasasever, Derya Duranyıldız, Süleyman Bademler, Hilal Oğuz Soydinç

“…Gene expression levels between the two groups were analyzed by the qPCR (quantitative Polymerase Chain Reaction) method. …”
Get full text

Efeitos do Orforglipron no tratamento da obesidade by Marcelo José da Silva de Magalhães, Pedro Henrique Martins de Souza, Victória Toledo Oliva Ferreira Silva

“…Para a confecção da metanálise, foi utilizado modelo de efeito fixo e testes de heterogeneidade e para a análise de heterogeneidade, foi utilizado o teste Q de Cochran e o I². Foi avaliada a perda de peso absoluta por meio do uso do Orforglipron dose de 45 mg diária versus placebo. …”
Get full text

Serum Levels of Mitochondrial Fission- and Fusion-Related Genes of Coal Workers’ Pneumoconiosis and Risk Factor Analysis Based on a Generalized Linear Model by Xiao-Yu Li, Jing-Lin Wei, Yong-Xin Xie, Ji Zhao, Li-Ya Ma, Na Zhang, Hui-Fang Yang

“…The mRNA expression levels of OPA1, Drp1, MFN2, Fis1, Col I, Col III, and α-SMA were determined by q-PCR. The protein expression levels of OPA1, Drp1, MFN2, Fis1, Col I, Col III, and α-SMA were detected by western blot. …”
Get full text

Haemophilus parasuis Subunit Vaccines Based on Native Proteins with Affinity to Porcine Transferrin Prevent the Expression of Proinflammatory Chemokines and Cytokines in Pigs by R. Frandoloso, S. Martínez-Martínez, E. F. Rodríguez-Ferri, S. Yubero, D. Rodríguez-Lázaro, M. Hernández, C. B. Gutiérrez-Martín

“…The expression of chemokines (CCL-2 and CXCL-8) and cytokines (IL-1α, IL-1β, IL-6, TNF-α, and IL-10) was evaluated by RT-qPCR in colostrum-deprived pigs vaccinated and challenged with Haemophilus parasuis serovar 5. …”
Get full text

A Framework for Coxeter Spectral Classification of Finite Posets and Their Mesh Geometries of Roots by Daniel Simson, Katarzyna Zając

“…We are mainly interested in a Coxeter spectral classification of posets J such that the symmetric Gram matrix GJ:=(1/2)[CJ+CJtr]∈𝕄J(ℚ) is positive semidefinite, where CJ∈𝕄J(ℤ) is the incidence matrix of J. …”
Get full text