A New Reference Plasmid “pGMT27” Provides an Efficient Transgenic Detection Method for Flue-Cured Tobacco by Jing Yu, Xiaolian Zhang, Muhammad Faheem Adil, Bo Lei, Mengao Jia, Huina Zhao, Shizhou Yu, Jiemin Liu, Yushuang Guo, Imran Haider Shamsi

“…In the quantitative real-time PCR (qPCR) assays with pGMT27 as a calibrator, the reaction efficiencies for P-35S and NR were 101.427% and 98.036%, respectively, whereas the limit of detection (LOD) and limit of quantification (LOQ) were 5 copies and 10 copies per reaction. …”
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The Mediation of Circulating Inflammatory Proteins in the Causal Pathway from Immune Cells to COPD by Yan K, Wang Y, Xin P

“…Subsequently, sensitivity analyses were conducted, employing Cochran’s Q test to assess heterogeneity, MR-PRESSO and MR-Egger tests to assess pleiotropy, and reverse MR and Steiger directionality tests to rule out reverse causality. …”
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Quem cala, consente? Um estudo com trabalhadores terceirizados em uma Universidade Pública by Adolfo de Alencar Melo Júnior, Diogo Henrique Helal, Síria Freire de Paula

“…Os dados foram analisados por meio de Análise de Conteúdo associada à Análise Lexicométrica com o auxílio do software IRaMuTeQ, deixando evidente que a aceitação da regra (ou norma) se dá apenas enquanto norteadora da atividade. …”
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Potential Pathways Involved in Elaidic Acid Induced Atherosclerosis in Human Umbilical Vein Endothelial Cells by Huahong Yu, Xiangmei Li, Zhongshang Liang, Bin Qiu, Siguang Li, Ting Luo, Jing Li, Hongyan Li, Zeyuan Deng

“…The expressions of some selected proteins (PSME3, XRCC5, GSTP1, and GSTO1) were validated by qRT-PCR analysis. Western blotting analysis further confirmed that elaidic acid downregulated the expression of PSME3 and XRCC5. …”
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Identification of retinol dehydrogenase 10 as a shared biomarker for metabolic dysfunction-associated steatotic liver disease and type 2 diabetes mellitus by Fangyu Li, Rui Li, Hongjun Deng

“…Next, hub gene validation was performed using the least absolute shrinkage and selection operator (LASSO) and receiver operating characteristic (ROC) curves. Finally, we used RT-qPCR, immunofluorescence, Western blotting and Elisa to validate hub gene expression in MASLD and T2DM mouse models.ResultsThis analysis identified 20 genes shared by MASLD and T2DM that were enriched in the bile secretion, phototransduction, cancer, carbohydrate digestion and absorption, cholesterol/glycerol metabolism, and retinol metabolism. …”
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Experimental Study on Undrained Shear Properties of Saline Soil under Freeze-Thaw Cycles by Shukai Cheng, Qing Wang, Jiaqi Wang, Yan Han

“…Under the action of freeze-thaw cycles, on the p′−q plane of the stress space, the effective stress path gradually moves to the lower left side. …”
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Thymosin β4 Protects against Cardiac Damage and Subsequent Cardiac Fibrosis in Mice with Myocardial Infarction by Fei Wang, Yajuan He, Naijuan Yao, Litao Ruan, Zhen Tian

“…Real-time quantitative reverse-transcription PCR (qRT-PCR), immunohistochemistry (IHC), and Western blot were used to analyze Tβ4 expression in acute myocardial infarction (AMI) cardiac tissues. …”
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Identification of Iron Homeostasis Genes Dysregulation Potentially Involved in Retinopathy of Prematurity Pathogenicity by Microarray Analysis by Xian-qiong Luo, Chun-yi Zhang, Jia-wen Zhang, Jing-bo Jiang, Ai-hua Yin, Li Guo, Chuan Nie, Xu-zai Lu, Hua Deng, Liang Zhang

“…The one-class algorithm in significance analysis of microarray (SAM) software was used to screen the differentially expressed genes (DEGs) and quantitative RT-PCR (qRT-PCR) was conducted to validate the results. …”
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Exploring Anti-Breast Cancer Effects of Live Pediococcus acidilactici and Its Cell-Free Supernatant Isolated from Human Breast Milk by Naa N. Adumuah, Jude T. Quarshie, Harry Danwonno, Anastasia R. Aikins, Elmer N. Ametefe

“…For in vitro experiments, we used isolated P. acidilactici to treat MDA-MB-231 cells, and an MTT assay was used to detect proliferation. RT-qPCR and wound healing assays were performed to determine the effect of the isolated P. acidilactici on breast cancer cytokine expression and migration. …”
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Nb-FAR-1: A key developmental protein affects lipid droplet accumulation and cuticle formation in Nippostrongylus brasiliensis. by Wenmin Qi, Fei Shen, Chuyue Wang, Juan Wen, Xi Pan, Zhongying Zhao, Lihua Xiao, Yaoyu Feng, Dongjuan Yuan

“…Nb-FAR-1 was highly expressed throughout developmental stages by RNA-seq data and qPCR analyses, and Nb-FAR-1 was a secretory protein and abundant in the excretory-secretory products. …”
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Exploring Inflammatory Changes in the Peripheral Blood of Type 2 Diabetes Mellitus in China by Li D, Zhang Z, Li W, Zhao C, Li X, Pan Z, Li Y

“…Flow cytometry, Western Blotting and qPCR were used to analyse the apoptosis of monocytes or THP-1 cells.Results: The numbers of white blood cells, lymphocytes, and neutrophils substantially increased with elevated IL-6 levels. …”
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Exosomes Derived from SW480-Resistant Colon Cancer Cells Are Promote Angiogenesis via BMP-2/Smad5 Signaling Pathway by Song Yang, Lei Yao, Xiaolong Wang, Hao Sun, Chaogang Du, Chengpeng Song, Jingyu Fu, Yongjun Wu, Hongwu Huang, Chuansi Wang, Yongsen Wang, Yixiang Xie

“…The CCK-8 assay, migration assay, tube formation assay, qPCR, and Western blotting were performed in human umbilical vein endothelial cells (HUVECs). …”
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A high coverage reference transcriptome assembly of pea (<i>Pisum sativum</i> L.) mycorrhizal roots by A. M. Afonin, I. V. Leppyanen, O. A. Kulaeva, O. Y. Shtark, I. A. Tikhonovich, E. A. Dolgikh, V. A. Zhukov

“…Genes specific to mycorrhizal roots were found in the assembly, their expression patterns were examined with qPCR on two pea cultivars, Frisson and Finale. …”
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Evolution and importance of Molecular Microbiology in the Laboratory of the Provincial Center for Hygiene, Epidemiology and Microbiology by María de Lourdes Sánchez Alvarez, Rafael Abreu Duarte, Edenis García Alvarez, Adrian Fernández García, Ermis Alexander González Martínez, Maidelys Mendoza Acosta

“…Results: In 2018, molecular diagnosis was implemented with two platforms, Rotor Gene-Q® and Gene Xpert® MTB/RIF, and in 2021, four were available, when the SLAN and the COBAS 4800® platform were introduced. …”
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Optimizing Laser Capture Microdissection Protocol for Isolating Zone-Specific Cell Populations from Mandibular Condylar Cartilage by Aisha M. Basudan, Yanqi Yang

“…Then, to evaluate LCM-RNA integrity, 3′/m ratios of glyceraldehyde 3-phosphate dehydrogenase (GAPDH) and beta-actin (β-Actin) using quantitative reverse transcription-polymerase chain reaction (qRT-PCR) were calculated. Both fixed and unfixed tissue sections allowed reliable identification of MCC zones. …”
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Association of body roundness index with cardiovascular disease in patients with cardiometabolic syndrome: a cross-sectional study based on NHANES 2009-2018 by Xiaohua He, Jingling Zhu, Wenfei Liang, Xiuling Yang, Weimin Ning, Zhan Zhao, Jingyi Chen, Qiuxing He

“…The highest quartile of BRI (Q4) showing the strongest association with CVD. …”
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β-Catenin Regulation in Sporadic Colorectal Carcinogenesis: Not as Simple as APC by Ernst Fredericks, Gill Dealtry, Saartjie Roux

“…Activation of PPARγ has inhibitory effect on β-catenin. Methods. Using qPCR and IHC, we compared β-catenin, PPARγ, COX-2, and IL-17A in the colonic mucosa of patients with sporadic CRC, inflammatory bowel disease (IBD), and irritable bowel syndrome (IBS), against a normal control population. …”
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Are We Overlooking Anatomical Contributions to Dynamic Knee Valgus? by Matt Dewald, Madison Andersen, Laura Higgins, Emma Porter, Alex Wickersham

“…Static anatomical measurements of static knee valgus and Q-angle had a weak positive correlation with DKV measurements for subjects' natural jumps (r=.37, p\<0.01 and r=.34, p=0.04, respectively). …”
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Identification of potential hydropower generation sites using geospatial techniques in the Megecha watershed of Ethiopia by Habtamu Wimego Anore, Tarun Kumar Lohani, Abebe Temesgen Ayalew

“…Based on the flow duration curve, the minimum flow of the river for the percent of exceedance of 90 % (Q90) for the sites 1, 2, and 3 are 0.37 m3/s, 0.45 m3/s, and 0.48 m3/s, respectively. …”
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YTHDF2 mediates the protective effects of MG53 on myocardial infarction injury via recognizing the m6A modification of MG53 by Zhaojie Li, Kai Li, Jianqiang Zhao

“…MG53 expression was detected using RT-qPCR, and its m6A levels were measured using Me-RIP. …”
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