Propofol Ameliorates Microglia Activation by Targeting MicroRNA-221/222-IRF2 Axis by Xi Xiao, Yuanyuan Hou, Wei Yu, Sihua Qi

“…However, the underlying molecular mechanism remains largely unknown. Methods. Real-time qPCR, enzyme-linked immunosorbent assay, and Western blotting were used to identify the expression pattern of miR-221/222, inflammatory genes, cytokines, and IRF2. …”
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Pan-genome analysis and expression verification of the maize ARF gene family by Quan-cai Man, Yan-qun Wang, Shun-juan Gao, Zhi-chang Gao, Zheng-ping Peng, Jiang-hui Cui

“…Further combining the transcriptome data and RT-qPCR found that, The expression levels of ARF family members in maize were higher at the early stage of embryo and grain development, and the expression levels of each member in embryo and grain were complementary, and the ARF4 plays an important role in abiotic stress. …”
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Dietary fiber content in clinical ketogenic diets modifies the gut microbiome and seizure resistance in mice by Ezgi Özcan, Kristie B. Yu, Lyna Dinh, Gregory R. Lum, Katie Lau, Jessie Hsu, Mariana Arino, Jorge Paramo, Arlene Lopez-Romero, Elaine Y. Hsiao

“…Supplementing with seizure-protective fibers enriches microbial genes related to queuosine biosynthesis and preQ0 biosynthesis and decreases genes related to sucrose degradation and TCA cycle, which are also seen in seizure-protected mice that are fed fiber-containing ketogenic formulas. …”
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A method for siRNA-mediated knockdown of target genes in RA-induced neurogenesis using P19 cells by Hossein Khodadadi, Hiroaki Taniguchi

“…Validation of the differentiation was performed through quantitative reverse transcription PCR (RT-qPCR) analysis, measuring the expression levels of key neuronal markers, including Map2 and Pax6 along with the pluripotency marker Oct4. …”
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Simultaneous Quantification of Dexpanthenol and Resorcinol from Hair Care Formulation Using Liquid Chromatography: Method Development and Validation by Amit Kumar De, Partha Pratim Chowdhury, Shyamaprasad Chattapadhyay

“…The method has been validated as per ICH Q2(R1) guidelines. The ease of single step sample preparation, accuracy, and precision (intraday and interday) study presents the method suitable for the simultaneous quantification of dexpanthenol and resorcinol from any personal care product and dermatological preparations containing these two ingredients.…”
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RNA-Binding Proteins and Alternative Splicing Genes Are Coregulated in Human Retinal Endothelial Cells Treated with High Glucose by Hongran Zhao, Hui Kong, Bozhao Wang, Sihui Wu, Tianran Chen, Yan Cui

“…Furthermore, the expression of differentially expressed RBPs was validated by qRT-PCR in mouse retinal microvascular endothelial cells and retinas from the streptozotocin mouse model. …”
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Sophocarpine inhibits the proliferation and induces apoptosis of glioblastoma cells through regulating the miR-21/PTEN/PI3K/AKT axis by Feng Si, Qian Wang, Fei Chen, Xiangdong Lu

“…CCK-8 was applied to analyze cell toxicity and proliferation. qRT-PCR and western blot were used to measure RNA and protein expressions, respectively. …”
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The Effect of Autologous Platelet-Rich Gel on the Dynamic Changes of the Matrix Metalloproteinase-2 and Tissue Inhibitor of Metalloproteinase-2 Expression in the Diabetic Chronic R... by Lan Li, Dawei Chen, Chun Wang, Guanjian Liu, Xingwu Ran

“…The expression of MMP-2 and TIMP-2 as well as transforming growth factor-β1 (TGF-β1) in the granulation tissue was detected by q TR-PCR and IHC. The relationship between the expression level of MMP-2 and TIMP-2 and their ratio and that of TGF-β1 was analyzed. …”
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In-silico screening and analysis of missense SNPs in human CYP3A4/5 affecting drug-enzyme interactions of FDA-approved COVID-19 antiviral drugs by Amro A. Abdelazim, Mohamad Maged, Ahmed I. Abdelmaksoud, Sameh E. Hassanein

“…Our findings indicated that the CYP3A4 gene variants; R418T, I335T and R130P and the CYP3A5 gene variants; I335T, L133P and R130Q are considered the most deleterious missense SNPs. …”
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Functional study on the regulation of adventitious root formation by PpSAT4 gene in peach rootstock ‘GF 677’ by HAO Lanlan, WANG Hong, ZHANG Fan, ZHANG Xuebing, YANG Yang

“…Analysis of cis-acting elements showed the presence of response elements such as light, auxin, and gibberellin in the PpSAT4 sequence. (2) The overexpression vector Pp- SAT4-CAM-FLAG was constructed and transformed P . domestica and A . thaliana, and the transgenic lines were obtained. (3) qRT-PCR analysis showed that the expression level of PpSAT4 in the transgenic lines was higher than that of the wild-type. …”
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Adaptive evolution of SARS-CoV-2 during a persistent infection for 521 days in an immunocompromised patient by Hanno Schmidt, Lea Schick, Jürgen Podlech, Angélique Renzaho, Bettina Lieb, Stefan Diederich, Thomas Hankeln, Bodo Plachter, Oliver Kriege

“…Amongst them were the substitutions S:K417T, S:N440S, S:K444R, S:V445A, S:G446N, S:L452Q, S:N460K, and S:E484V at positions that are notorious for their resistance-mediating effects. …”
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Optimization of the intron sequences combined with the CMV promoter increases recombinant protein expression in CHO cells by Ting-Ting Xu, Yan-Fang Wang, Jing-Jia Yuan, Chun-Liu Mi, Shao-Lei Geng, Xiao-yin Wang, Tian-Yun Wang

“…The results showed that the truncated, EF-1α first, chimeric, and β-globin introns can significantly improve stable transgene expression in CHO cells. The qPCR results indicated that the mRNA level of transgene increased through optimizing intron sequences combined with the CMV promoter. …”
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Substantial Variation Exists in Clinical Exposure to Chief Complaints Among Residents Within an Emergency Medicine Training Program by Corlin M. Jewell, Amy T. Hummel, Dann J. Hekman, Benjamin H. Schnapp

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Decreased Expression of GPER1 Gene and Protein in Goiter by Raquel Weber, Ana Paula Santin Bertoni, Laura Walter Bessestil, Ilma Simoni Brum, Tania Weber Furlanetto

“…We investigated GPER1 gene and protein expressions in normal thyroid and goiter using reverse transcription quantitative polymerase chain reaction (RT-qPCR) and Western blot, respectively. In normal thyroid (n=16) and goiter (n=19), GPER1 gene was expressed in all samples, while GPER1 protein was expressed in all samples of normal thyroid (n=15) but in only 72% of goiter samples (n=13). …”
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Pilot study: Initial investigation suggests differences in EMT-associated gene expression in breast tumor regions by Kylie L. King, Hamed Abdollahi, Zoe Dinkel, Alannah Akins, Homayoun Valafar, Heather Dunn

“…We selected four regions of interest from tumor biopsy samples and used laser microdissection to extract tissue for gene expression characterization via RT-qPCR. Both parametric and non-parametric statistical analyses identified genes differentially expressed between the two ethnic groups. …”
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SPARQ: Efficient Entanglement Distribution and Routing in Space–Air–Ground Quantum Networks by Mohamed Shaban, Muhammad Ismail, Walid Saad

“…To solve the entanglement routing problem, a deep reinforcement learning (RL) framework is proposed and trained using deep Q-network (DQN) on multiple graphs of SPARQ to account for the network dynamics. …”
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CDR-Detector: a chronic disease risk prediction model combining pre-training with deep reinforcement learning by Shaofu Lin, Shiwei Zhou, Han Jiao, Mengzhen Wang, Haokang Yan, Peng Dou, Jianhui Chen

“…Aiming at this challenge, this study combines EHR based pre-training and deep reinforcement learning to develop a novel chronic disease risk prediction model called CDR-Detector. The model adopts the Q-learning architecture with a custom reward function. …”
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Sufentanil enhances the cortical neurogenesis of rats with traumatic brain injury via PI3K/AKT signal pathway by Wei Gu, Mimi Wu, Ruocui Zhang, Peiyu Liu, Yang Jiao, Hui Rong

“…The oxidative stress, inflammation, nerve cell damage, melatonin, brain-derived neurotrophic factor (BDNF), neuron regeneration and p-AKT protein level in the cortex were detected with ELISA, TUNEL, qRT-PCR, immunofluorescence and Western blot. Pain behavioral test was assessed with mechanical withdrawal threshold (MWT). …”
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Origin of Circulating Free DNA in Sepsis: Analysis of the CLP Mouse Model by Shigeto Hamaguchi, Yukihiro Akeda, Norihisa Yamamoto, Masafumi Seki, Kouji Yamamoto, Kazunori Oishi, Kazunori Tomono

“…Increased cf-DNA levels were observed only in CLP mice and not in the control groups; the qPCR findings revealed that the cf-DNA was mainly host-derived, even in bacteremic conditions. …”
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A transgenic mouse line with a 58 kb fragment deletion shows skeletal defects by Ahmed Alamoudi, Dina Husein, Yoshio Ohyama, Angela Chen, Hanna Mochida, Yoshiyuki Mochida

“…Histological examination of the 58 kb-/- growth plate demonstrated disorganized chondrocyte zones and extended hypertrophic zone. The qPCR results showed downregulation of several osteoblast differentiation markers in the 58 kb-/- long bone. …”
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