Nobiletin restores the intestinal barrier of HFD-induced obese mice by promoting MHC-II expression and lipid metabolism by Ni Yang, Yue-Shan Pang, Yali Zheng, Yan-Ju Gong, Wei-Jun Ding

“…Hematoxylin and eosin (HE) staining and immunofluorescence were used to observe the intestinal barrier. RT-qPCR was used to determine the transcriptomic levels of genes involved in intestinal barrier integrity and lipid metabolism. …”
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Effect of Sterilization Methods on the Storage Quality of Soybean Protein-based Small Crisp Meat and Its Shelf Life Prediction by Ying SUN, Bin ZHOU, Long WANG, Shen LIU, Binglin LU, Lianzhou JIANG, Juyang ZHAO, Xiuqing ZHU

“…The study compared the effects of microwave sterilization and water bath sterilization on the total number of colonies, and investigated the impact of different storage temperatures on the physical and chemical properties and texture characteristics of the product. The Q10 model was utilized to obtain the shelf life model of plant-based small crispy meat precooked dish based on the TVB-N value, and predicted its shelf life at 4 ℃ storage temperature. …”
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The PTP1B Inhibitor Trodusquemine (MSI-1436) Improves Glucose Uptake in Equine Metabolic Syndrome Affected Liver through Anti-Inflammatory and Antifibrotic Activity by Lynda Bourebaba, Anna Serwotka-Suszczak, Nabila Bourebaba, Magdalena Zyzak, Krzysztof Marycz

“…In this study, the outcomes of PTP1B inhibition using trodusquemine (MSI-1436) on key equine metabolic syndrome (EMS)-related alterations including inflammation, fibrosis, and glucose uptake have been analyzed in liver explants collected from EMS-affected horses using various analytical techniques, namely, flow cytometry, RT-qPCR, and Western blot. Results. PTP1B inhibition using trodusquemine resulted in decreased proinflammatory cytokines (IL-1β, TNF-α, and IL-6) release from liver and PBMC affected by EMS and regulated expression of major proinflammatory microRNAs such as miR-802 and miR-211. …”
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Diagnóstico prenatal de atresia duodenal en feto con Síndrome Down. A propósito de un caso by Pedro Alí Díaz-Véliz Jiménez, Belkis Vidal Hernández, Iliana González Santana, Doris Portero Cabrera, Leydi María Sosa Águila

“…Por sospecha de asociación de este tipo de malformación con enfermedad cromosómica, se le realizó diagnóstico prenatal citogenético, cuyo resultado fue: 47,XX, +21, inv(9)(p11;q12), feto femenino positivo para una trisomía 21 e inversión pericéntrica del cromosoma 9. …”
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Alterations in mRNA level of proteins related to redox state and mitochondria in an Alzheimer’s disease animal model: Promising targets in neuroprotection by Sylwia Żulińska, Przemysław L. Wencel, Kinga Czubowicz, Joanna B. Strosznajder

“…We observed significant decreases in mRNA of Sod2, Parp1 and Sirt1 in the 3-month-old AD Tg mice and upregulation of Parp1 in the 6-month-old AD Tg mice by qPCR analysis. Then, mt-CytB and mt-Co1 (complex III and IV) mRNA levels were increased in 12- and 6-month-old AD brains, respectively. …”
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Hybridize Machine Learning Methods and Optimization Techniques to Analyze and Repair Welding Defects via Digital Twin of Jidoka Simulator by Ahmed M. Abed, Tamer S. Gaafar

“…Four clusters are used to identify welding flaws&#x2019; severity to keep them from getting worse via mapping their finite element meshes (MFEM) and using the high-frequency current ring (HFCR) lean technique to identify their size, depth, growth direction (<inline-formula> <tex-math notation="LaTeX">$\boldsymbol {{R}_{s}}$ </tex-math></inline-formula>), and shape deployment via prediction to assign the flaws as Susceptible (S), and preludes to transfer to a Quarantine cluster (Q) to take one decision whether rescued (R), Isolated (I) or Eliminated (E). …”
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Transcriptome of the Deep-Sea Black Scabbardfish, Aphanopus carbo (Perciformes: Trichiuridae): Tissue-Specific Expression Patterns and Candidate Genes Associated to Depth Adaptatio... by Sergio Stefanni, Raul Bettencourt, Miguel Pinheiro, Gianluca De Moro, Lucia Bongiorni, Alberto Pallavicini

“…A selection of putative genes was standardized to evaluate the correlation between number of contigs and their normalized expression, as determined by qPCR amplification. The screening of the libraries contributed to the identification of new EST simple-sequence repeats (SSRs) and to the design of primer pairs suitable for population genetic studies as well as for tagging and mapping of genes. …”
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Collaboration between general practitioners and social workers: a scoping review by Birgitta Weltermann, Patrick Mark, Samira Sommer, Cornelia Löwe

“…Case management as person-centred care for defined patient groups is a central aspect of all identified collaborations between general practitioners and social workers.Conclusion This scoping review showed evidence for benefits on behalf of patients, professionals and healthcare systems by collaborations between general practitioners and social workers, yet more rigorous research is needed to better understand the impact of these collaborations.Trial registration number www.osf.io/w673q.…”
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TSC complex decrease the expression of mTOR by regulated miR-199b-3p by Na Zhao, Qiuhong Xiong, Ping Li, Guangxin Chen, Han Xiao, Changxin Wu

“…In this study, besides a reported variation, c.2509_2512del (p.Asn837Valfs*11, p.N837fs) in TSC1, we found a de novo TSC2 variation c.1113delG (p.Gln371Hisfs*18, p.Q371fs), which these two mutation influence the formation of TSC complex. …”
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Immediate-Early Genes and Delayed Primary Response Genes Regulated by NmU in SKBR3 HER-2 Positive Breast Cancer Cell Line by Jessica Murphy, Sweta Rani

“…AimThe aim of this study was to investigate the effects of NmU on the regulation of immediate early and delayed primary response genes in HER2-positive SKBR3 breast cancer cells using RT-qPCR gene expression analysis. This information was then used to uncover related pathways that may be involved in the progression of this aggressive cancer due to NmU. …”
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Integration of transcriptomics and metabolomics data revealed role of insulin resistant SNW1 gene in the pathophysiology of gestational diabetes by Ravi Bhushan, Rimjhim Trivedi, Ritu Raj, Anjali Rani, Sangeeta Rai, Anima Tripathi, Shiv Govind Rawat, Ajay Kumar, Dinesh Kumar, Pawan K. Dubey

“…High-throughput RNA sequencing and computational bioinformatics analyses were conducted to identify novel hub genes, and their regulatory mechanisms were validated through qRT-PCR, western blot, and siRNA-mediated knockdown studies. …”
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The Single-Nucleotide Polymorphism (SNP) Validity to Detect Omicron Variants by Lia Gardenia Partakusuma, Luhung Budiailmiawan, null Budiman, Ida Parwati, null Aryati, Basti Andriyoko, Louisa Markus, Corine Niswara, Cut Nur Cinthia Alamanda

“…The SNP method can be a more time-efficient alternative to detect omicron variants of SARS-CoV-2 and distinguish their sublineages (BA.1, BA.2, and BA.4/5) by two different specific gene mutations in combination analysis (ΔH69/V70 and Q493R mutations).…”
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A Fractional-Order Model of Biopolyester Containing Naturally Occurring Compounds for Soil Stabilization by Nadka Tzankova Dintcheva, Marilena Baiamonte, Rosalia Teresi, Gioacchino Alotta, Emanuela Bologna, Massimiliano Zingales

“…This research was conducted to determine the effect of naturally occurring compounds, such as quercetin (Q) and sodium montmorillonite (NaMMt) at different weight ratios, in biopolyester, such as polylactic acid (PLA), aiming to formulate ecosustainable materials to control the soil degradation and to protect the environment. …”
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Diagnostic value of miR-193a-5p in severe pneumonia and its correlation with prognosis by Xiaoji Su, Lijuan Zhu, Jiajia Zhuo, Shihai Zhang

“…Serum miR-193a-5p levels were detected by RT-qPCR. The correlation of miR-193a-5p with CRP, WBC, neutrophil count, and NLR was assessed by Spearman analysis. …”
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circRNA_101277 Influences Cisplatin Resistance of Colorectal Cancer Cells by Modulating the miR-370/IL-6 Axis by Qing Lv, Qinghua Xia, Anshu Li, Zhiyong Wang

“…The expression of circRNA_101277, miR-370, and IL-6 was assessed via qRT-PCR. IL-6 levels were measured with a human IL-6 ELISA kit based on the provided protocols. …”
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Analysis and detection of temperature inductions of land cover changes in Lorestan province using MODIS sensor products by hamed heidari, darush yarahmadi, hamid mirhashemi

“…In this regard, the data of land cover classes of MCD12Q2 composite product and ground temperature of MOD11A2 product of MODIS sensor were used. …”
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The relationship between compassion fatigue, engagement and decent work among nurses: a cross-sectional study by Yaping Feng, Sui Li, Weilan Ma, Yaqian Chen, Jingxuan Zhang, Hong Luo, Bowen Xue

“…We employed a comprehensive set of assessment tools, including an evaluation of demographic characteristics, the Decent Work Perception Scale (DWPS), the Chinese version of the Compassion Fatigue Short Scale (C-CFSS), and the Gallup Q12 survey scale. Results The findings of this study demonstrated that compassion fatigue was significantly negatively correlated with both decent work (r = -0.482, p < 0.001) and engagement (r = -0.461, p < 0.001). …”
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Resveratrol Enhances Cardiomyocyte Differentiation of Human Induced Pluripotent Stem Cells through Inhibiting Canonical WNT Signal Pathway and Enhancing Serum Response Factor-miR-1... by Hui Liu, Shaoli Zhang, Lihua Zhao, Yan Zhang, Qiuping Li, Xiaoyan Chai, Yongchun Zhang

“…Under embryoid bodies (EBs) condition, the effect of RSV on the change of pluripotent markers, endoderm markers, mesoderm markers, and ectoderm markers was measured using qRT-PCR. Under CM differentiation culture, the effect of RSV on CM specific markers was also measured. …”
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Prediction and analysis of disease burden of mental disorders in China from 1990 to 2021 by JIANG Lu, ZHANG Zhidong, WU Jianjun, LIU Lu, SHANG Longjian, WEI Xingmin

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Aqp 9 and Brain Tumour Stem Cells by Guri Fossdal, Einar O. Vik-Mo, Cecilie Sandberg, Mercy Varghese, Mari Kaarbø, Emily Telmo, Iver A. Langmoen, Wayne Murrell

“…We have compared these to the situation in normal rat brain, its stem cells, and differentiated cells derived thereof. In short, qPCR in tumour tissue showed presence of aqp1, 4, and 9. …”
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