Identification of cell chemistries in lithium-ion batteries: Improving the assessment for recycling and second-life by Christopher Wett, Jörg Lampe, Dominik Görick, Thomas Seeger, Bugra Turan

“…Using the Python Battery Mathematical Modelling (PyBaMM) framework, synthetical partial open circuit voltage (OCV) charge and discharge curves are generated with an electrochemical single particle model for three different cathode chemistries and the initial state of charge and state of health values as well as the initial capacities are varied. The dV/dQ characteristics are chosen as features and four machine learning algorithms are trained on different lengths of OCV curves. …”
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20-HETE mediates Ang II-induced cardiac hypertrophy via ROS and Ca2+ signaling in H9c2 cells by Jingyi Han, Jiaojiao Li, Lianlian Liu, Kaiyuan Li, Chun Zhang, Yong Han

“…This study investigated the effects of Ang II on CYP4A expression and 20-HETE production in H9c2 cells using RT-qPCR, Western blot, and ELISA. The role of 20-HETE in Ang II-induced cardiac hypertrophy was examined using DHE, MitoSOX, and JC-1 staining to evaluate reactive oxygen species (ROS) generation and mitochondrial membrane potential changes. …”
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Advancements in nanosensors for cancer detection by Dinesh Bhatia, Tania Acharjee, Monika Bhatia

“…The accuracy and usability of detection are further improved by advancements in wearable technologies, microfluidics, and electrochemical and graphene-based sensors. BrCyS-Q and NanoLiposomes provide improved photodynamic treatment and targeted medication delivery, respectively. …”
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MicroRNA-29b Suppresses Inflammation and Protects Blood-Brain Barrier Integrity in Ischemic Stroke by Xiaoqing Ma, Ho Jun Yun, Kenneth Elkin, Yunliang Guo, Yuchuan Ding, Guangwen Li

“…Methods. miR-29b from stroke patients and rats with middle cerebral artery occlusion (MCAO) were assessed using real-time polymerase chain reaction (PCR). miR-29b agomir was used to increase miR-29b expression in leukocytes via intravenous injection. C1q and tumor necrosis factor (C1QTNF) 6, interleukin- (IL-) 1β, zonula occludens- (ZO-) 1, occludin, and ischemic outcomes were assessed in MCAO rats. …”
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Influence of endosymbionts on the reproductive fitness of the tick Ornithodoros moubata by Taraveau, Florian, Pollet, Thomas, Duhayon, Maxime, Gardès, Laëtitia, Jourdan-Pineau, Hélène

“…Similarly, 366 first stage nymphs of O. moubata were exposed to the same treatments for molecular quantification of both endosymbionts. Results from qPCR suggested that the treatments had a bacteriostatic effect on endosymbionts without completely eliminating neither Francisella-like endosymbiont nor Rickettsia. …”
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Long non-coding RNA TINCR suppresses growth and epithelial-mesenchymal transition by inhibiting Wnt/β-catenin signaling pathway in human pancreatic cancer PANC-1 cells: Insights fr... by Wei Yuan, Zhu Ping

“…Quantitative real-time polymerase chain reaction (qRT-PCR) was used to investigate TINCR expression in pancreatic cancer cells. …”
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KLF5 promotes esophageal squamous cell carcinoma radioresistance by targeting the Keap1-Nrf2 pathway by Yang Wang, Yue-ying Yang, Abulajiang Kamili, Dilimulati Aishanjiang, Yi Liu

“…KLF5 expression was detected by RT-qPCR. The effects of overexpression or downregulation of KLF5 on anti-irradiated cells' proliferation, migration, invasion, and apoptotic activity were studied through colony formation assay, Transwell assay, and flow cytometry. …”
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Comparing the Gene Expression Profile of Stromal Cells from Human Cord Blood and Bone Marrow: Lack of the Typical “Bone” Signature in Cord Blood Cells by Julia Bosch, Amelie Pia Houben, Tatiana Hennicke, René Deenen, Karl Köhrer, Stefanie Liedtke, Gesine Kögler

“…The detailed gene expression analysis and overexpression of genes expressed differentially provide insight into the inherent capacity of stromal cells. Microarray and qRT-PCR analyses revealed closely related gene expression patterns of two stromal cell populations derived from CB. …”
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LncRNA HOTAIR: A Novel Biomarker for the Diagnosis of Asymptomatic Carotid Artery Stenosis and Prediction of the Onset of Cerebral Ischemic Events by Pengcheng Feng, Chenguang Tong, Yuan Li, Li Liu

“…Blood samples were collected and serum HOTAIR levels were detected by qRT-PCR. Logistic regression examined factors associated with the degree of carotid stenosis. …”
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Stachydrine targeting tumor-associated macrophages inhibit colorectal cancer liver metastasis by regulating the JAK2/STAT3 pathway by Yang Gui, Gengchen Xue, Yuyi Yuan, Jingbo Wang, Shuangjiao Deng, Fei Gao, Yushi Tian, Zhiqiang Zhao, Heng Fan

“…Subsequently, a series of assays, including quantitative reverse transcription polymerase chain reaction (qRT-PCR), flow cytometry, scratch, invasion, and tube formation assays, were conducted to confirm STA’s capacity to impede tumor cell migration, invasion, and angiogenesis in vitro. …”
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Uncovering glycolysis-driven molecular subtypes in diabetic nephropathy: a WGCNA and machine learning approach for diagnostic precision by Chenglong Fan, Guanglin Yang, Cheng Li, Jiwen Cheng, Shaohua Chen, Hua Mi

“…Finally, the expression patterns of these hub genes were validated using single-cell sequencing data and quantitative real-time polymerase chain reaction (qRT-PCR). Results Eleven GRGs showed abnormal expression in DN samples, leading to the identification of two distinct glycolysis clusters, each with its own immune profile and functional pathways. …”
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Correlation between uric acid levels and bone mineral density in patients with type 2 diabetes mellitus: a systematic review and meta-analysis by Hang Zhao, Cuijuan Qi, Yunjia Zhang, Luping Ren, Shuchun Chen

“…Heterogeneity was evaluated using the I2 and Q tests.ResultsThis meta-analysis included 10 studies comprising 5,717 patients with T2DM. …”
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Significant abundance of bacterial flagellin and expression of its surface localized receptor toll-like receptor 5 and cytokine interleukin-22 in South African infants with poor or... by Nontlantla J. Haindongo, Mapaseka Seheri, Cliff A. Magwira

“…The abundance of flagellin and expression of flagellin-encoding fliC, TLR5 and NLRC4, IL-22 and IL-18 genes was determined by qPCR. There were no differences in the abundance of flagellin between vaccine shedders and non-shedders (p = 0.15). …”
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Chemical Fingerprint and Metabolic Profile Analysis of Tianshu Tablets by Ultra-High Performance Liquid Chromatography/Quadrupole-Time of Flight Mass Spectrometry by Lin Chen, Renhao Chen, Hui Ouyang, Qi Wang, Zhifeng Li, Yulin Feng, Shilin Yang

“…In the present study, the chemical fingerprint of Tianshu tablets (TST) and the metabolic characteristics of compounds in rats after intragastric administration were studied by ultra-high performance liquid chromatography coupled with quadrupole-time of flight mass spectrometry (UPLC/Q-TOF MS). In a preliminary study, 77 chemical components in TST were determined by comparison with retention times, accurate molecular mass, and characteristic fragment ions of the known compounds in the literature and some well-known compounds were analyzed in detail, and the fragmentation pathways for parishins B, gastrodin A, and cnidilide or neocnilide were specifically analyzed. …”
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Seroprevalence of Seven Reproductive Diseases in Beef and Dairy Cows from Three Provinces in Indonesia by Didik Tulus Subekti, Mira Fatmawati, Arie Khoiriyah, Arum Pramesthi, Sulinawati Fong, Muhammad Ibrahim Desem, Zul Azmi, Eni Kusumaningtyas, Dwi Endrawati, Eko Setyo Purwanto

“…Seven reproductive diseases to be tested in parallel are neosporosis, chlamydiosis, brucellosis, Q fever, bovine viral diarrhea (BVD), infectious bovine rhinotracheitis (IBR), and BHV-4 infection. …”
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Temporal and spatial characterization of keratinocytes supporting orf virus replication by Byung-Joon Seung, Sushil Khatiwada, Daniel L. Rock, Gustavo Delhon

“…Using in situ hybridization, immunohistochemistry, confocal microscopy, qPCR, and a full-thickness wound/infection model in sheep, the natural host, we show that during an initial 2-day eclipse phase viral transcription and viral DNA replication are not detected. …”
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Effects of Aspirin on Odontogenesis of Human Dental Pulp Cells and TGF-β1 Liberation from Dentin In Vitro by V. Khampatee, C. Zhang, L. Chou

“…DSP and RUNX2 mRNA levels were measured with RT-qPCR. Alizarin red staining was conducted to evaluate the mineralized nodule formation. …”
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Discrete Element Study of Particle Size Distribution Shape Governing Critical State Behavior of Granular Material by Mingdong Jiang, Daniel Barreto, Zhi Ding, Kaifang Yang

“…A unique critical-state stress ratio in <i>p</i>′<i>-q</i> space is observed, indicating that the critical friction angle is independent of the shape of the PSD. …”
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Cytogenetic Evolution of Human Ovarian Cell Lines Associated with Chemoresistance and Loss of Tumorigenicity by Stéphanie Struski, Martine Doco‐Fenzy, Michael Koehler, Ilse Chudoba, Francis Levy, Linda Masson, Nicole Michel, Evelyne Ulrich, Nadine Gruson, Jean Bénard, Gérard Potron, Pascale Cornillet‐Lefebvre

“…However, these cell lines shared two common rearrangements: an unbalanced translocation der(8)t(8;13)(p22;q?) and a deletion of the 11p. These chromosomal imbalances could reflected the acquisition of the chemoresistance (der(8)) or the loss of tumorigenicity properties (del(11p)). …”
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PPARβ/δ Agonism with GW501516 Increases Myotube PGC-1α Content and Reduces BCAA Media Content Independent of Changes in BCAA Catabolic Enzyme Expression by Caroline N. Rivera, Jason S. Hinkle, Rachel M. Watne, Trent C. Macgowan, Andrew J. Wommack, Roger A. Vaughan

“…Metabolic gene and protein expression were assessed via quantitative real-time polymerase chain reaction (qRT-PCR) and western blot, respectively. Media BCAA content was assessed via liquid chromatography–mass spectrometry (LC/MS). …”
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