SIAE Rare Variants in Juvenile Idiopathic Arthritis and Primary Antibody Deficiencies by Eirini Sevdali, Elena Tsitsami, Maria Tsinti, Evangelia Farmaki, Efimia Papadopoulou-Alataki, Anastasios E. Germenis, Matthaios Speletas

“…Three novel SIAE variants (p.Q343P, p.Y495X, and c.1320+33T>C) were found only in patients with aJIA but interestingly also in their healthy relatives without autoimmunity, while none of PAD patients or their relatives carried SIAE defects. …”
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METTL1 coordinates cutaneous squamous cell carcinoma progression via the m7G modification of the ATF4 mRNA by Xinru Zhang, Tong Chen, Fanrong Zhang, Huanhuan Shi, Xiang Li, Zhijuan Wang, Dong Wang, Chao Hou

“…Mechanistically, through a combination of RNA sequencing, m7G methylated immunoprecipitation (MeRIP)-qPCR, and mRNA stability assays, we discovered that METTL1 is responsible for the m7G modification of ATF4 mRNA, leading to increased expression of ATF4. …”
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Renal Tissue-Derived Exosomal miRNA-34a in Diabetic Nephropathy Induces Renal Tubular Cell Fibrosis by Promoting the Polarization of M1 Macrophages by Shuai Zheng, Yi Zeng, Liqing Chu, Taiyang Gong, Sihong Li, Min Yang

“…After exosomal culture, macrophages are co-cultured with normal renal tubular cells to detect renal tubular cell fibrosis. Q-PCR and western blot were undertaken to detect related RNA and proteins. …”
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Meta-Analysis of Diagnostic Performance of Contrast-Fractional Flow Reserve versus Quantitative Flow Ratio for Functional Assessment of Coronary Stenoses by Ruitao Zhang, Jianwei Zhang, Lijun Guo

“…A bivariate model was applied to pool diagnostic parameters. Cochran’s Q test and the I2 index were used to assess heterogeneity and identify the potential source of heterogeneity by metaregression and sensitivity analysis. …”
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Metabolomics reveal the mechanism for anti-renal fibrosis effects of an n-butanol extract from Amygdalus mongolica by Gao Chen, Chang Hong, Zhou Hong-Bing, Liu Qing, Bai Ying-Chun, Liu Quan-Li, Bai Wan-Fu, Shi Song-Li

“…Serum metabolomics was assessed with HPLC-Q-TOF-MS. The BUT reduced levels of blood urea nitrogen, serum creatinine and albumin and lowered the content of malondialdehyde and hydroxyproline in tissues. …”
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Forecasting Rice Productivity and Production of Odisha, India, Using Autoregressive Integrated Moving Average Models by Rahul Tripathi, A. K. Nayak, R. Raja, Mohammad Shahid, Anjani Kumar, Sangita Mohanty, B. B. Panda, B. Lal, Priyanka Gautam

“…The autoregressive (p) and moving average (q) parameters were identified based on the significant spikes in the plots of partial autocorrelation function (PACF) and autocorrelation function (ACF) of the different time series. …”
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Multiomics-based assessment of the impact of airflow on diverse plant callus cultures by June-Sik Kim, Muneo Sato, Mikiko Kojima, Muchamad Imam Asrori, Yukiko Uehara-Yamaguchi, Yumiko Takebayashi, Thi Nhung Do, Thi Yen Do, Kieu Oanh Nguyen Thi, Hitoshi Sakakibara, Keiichi Mochida, Shinjiro Ogita, Masami Yokota Hirai

“…For each sample, gene expression was profiled with high-throughput RNA sequencing, 442 metabolites were measured using liquid chromatography (LC) with triple-quadrupole mass spectrometry (LC–QqQMS), and 31 phytohormones were quantified using ultra-performance LC coupled with a tandem quadrupole mass spectrometer equipped with an electrospray interface (UPLC-ESI-qMS/MS) and ultra-high-performance LC–orbitrap MS (UHPLC-Orbitrap MS). …”
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The design of experiments for the transcriptome studies by high-throughput sequencing methods by P. N. Menshanov, N. N. Dygalo

“…However, the optimal number of biological replicates per experimental group is equal to 5–8 (similar to the experiments quantifying the expression of single genes by qRT-PCR). For the transcriptome studies, it is recommended to use the sequencing technologies that have the accuracy of sequencing ≥ 0.999 per bp. …”
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Prophylactic effect of Kudingcha polyphenols on oxazolone induced colitis through its antioxidant capacities by Xingyao Long, Yanni Pan, Xin Zhao

“…It can also significantly reduce (p < 0.05) the activities of MPO, NO, MDA in mice colon tissues with colitis, increase the activity of GSH, and significantly increase (p < 0.05) the level of IL-2 and decrease the level of IL-10 in mice serum with colitis. The results of qPCR assay show that Kp can significantly increase (p < 0.05) the expression of Cu/Zn-SOD, Mn-SOD, GSH-Px, CAT, c-Kit, SCF and decrease the expression of IL-8, CXCR2 in mice colon tissues with colitis. …”
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Surgical Reimplantation for the Correction of Vesicoureteral Reflux following Failed Endoscopic Injection by Boris Chertin, Ksenia Prosolovich, Sagiv Aharon, Ofer Nativ, Sarel Halachmi

“…No new hydronephrosis or signs of obstruction developed in any of the patients. qDMSA renal scan was available in 8 patients showing improvement of function in 5 and stable function in 3, and no new scars were identified. …”
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Dulaglutide Protects Mice against Diabetic Sarcopenia-Mediated Muscle Injury by Inhibiting Inflammation and Regulating the Differentiation of Myoblasts by Fengyi Deng, Wenyan Wu, Xingyu Fan, Xing Zhong, Nuojin Wang, Yue Wang, Tianrong Pan, Yijun Du

“…In addition, inflammatory factors and FNDC5 in the muscle tissues were detected using qRT-PCR. Moreover, C2C12 cells were induced to enable their differentiation into skeletal muscle cells, and muscle factor levels were then detected. …”
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Distribution and environmental dissemination of antibiotic resistance genes in poultry farms and surrounding ecosystems by ShangMin Li, Kai Wang, DanPing Wang, HongZhi Wang, HuaXuan Zhao, JunHua Pu, KeHua Wang, ChunMei Li

“…We used 16S rRNA gene sequencing and HT-qPCR to analyze the characteristics of aerosol microbial communities and the abundance of ARGs. …”
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A Constitutively Mannose-Sensitive Agglutinating Salmonella enterica subsp. enterica Serovar Typhimurium Strain, Carrying a Transposon in the Fimbrial Usher Gene stbC, Exhibits Mul... by Kuan-Hsun Wu, Ke-Chuan Wang, Lin-Wen Lee, Yi-Ning Huang, Kuang-Sheng Yeh

“…The stbC mutant was resistant to several antibiotics, consistent with the finding that expression of yhcQ and ramA was enhanced. A complementation test revealed that transforming a recombinant plasmid possessing the stbC restored the mannose-sensitive agglutination phenotype to the stbC mutant much as that in the parental Salmonella enterica subsp. enterica serovar Typhimurium LB5010 strain, indicating the possibility of an interplay of different fimbrial systems in coordinating their expression.…”
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A Meta-Analysis for Association of Maternal Smoking with Childhood Refractive Error and Amblyopia by Li Li, Ya Qi, Wei Shi, Yuan Wang, Wen Liu, Man Hu

“…The heterogeneity was evaluated with the Chi-square-based Q statistic and the I2 test. Potential publication bias was finally examined by Egger’s test. …”
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Computed Tomography Enterography: Quantitative Evaluation on Crohn’s Disease Activity by Jingyun Cheng, Hui Xie, Hao Yang, Ke Wang, Guobin Xu, Guangyao Wu

“…The differences in bowel wall thickening, mural hyperenhancement in the portal vein period, and the ΔCT values were analyzed among groups using ANOVA (analysis of variance) and q test. Then, the parameters were correlated with SES-CD, C-reactive protein (CRP), and erythrocyte sedimentation rate (ESR). …”
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Endocervical miRNA-142 expression in patients with pelvic inflammatory disease positive for Chlamydia trachomatis by Aliaa Zara Allah Hussein Al-Tememy, Maysaa S. Alshukri, Asmaa Kadhim Gatea

“…Chlamydia trachomatis was determined by conventional polymerase chain reaction (PCR) whereas miRNA-142 was detected by RT-qPCR. Results: All 200 collected endocervical swab specimens were diagnosed by the gynecologists as having PID. …”
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lncRNA FR215775 Regulates Th2 Differentiation in Murine Allergic Rhinitis by Yue Ma, Le Shi, Keqing Zhao, Chunquan Zheng

“…To identify the effect of long noncoding RNA (lncRNA) FR215775 in regulating CD4+ T cells on murine models of allergic rhinitis (AR), the expression of lncRNA FR215775 in primary Th2 cells was detected through qRT-PCR. After knocking down the expression of lncRNA FR215775 via Sh-FR215775-Ads, Cell Counting Kit-8, cytometric bead array, and fluorescence-activated cell sorting were performed to determine its functions in vitro. …”
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Structural basis of CXCR4 assembly and regulation by Aijun Liu, Yezhou Liu, Richard D. Ye

“…Each protomer allosterically regulates the others, with Q272 in the ECL3 loop interacting with K38 (TM1) and V99 (TM2) of the adjacent protomer, resulting in a mutually inhibitory configuration. …”
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Transient ocular hypertension remodels astrocytes through S100B. by Weiran Huang, Kenji Matsushita, Rumi Kawashima, Susumu Hara, Yuichi Yasukura, Kaito Yamaguchi, Shinichi Usui, Koichi Baba, Andrew J Quantock, Kohji Nishida

“…Glial fibrillary acidic protein (GFAP), S100B, and actin staining were used to characterize astrocyte morphology and cytoskeleton, with qPCR used to measure mRNA expression. We also silenced S100B expression and conduct RNA sequencing on ONH astrocytes. …”
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Comparative Transcriptomic Analysis of Root Cadmium Responses in Two Chinese Rice Cultivars Yuzhenxiang and Xiangwanxian 12 by Shangdu Zhang, Zhenliang Luo, Xiang Wu, Bangzhi Shi, Huidan Jiang, Leliang Zhou, Lianyang Bai

“…Furthermore, we supposed that ADI1, CFBP1, PEX11-4, OsJ_019618, OsJ_24808, GDCSH, CLPB1, LAC6, and WNK3 might be implicated in Cd response based on the combined analysis of RT-qPCR, interaction, and GO annotation analysis. …”
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