Comprehensive RNA-seq analysis of benign prostatic hyperplasia (BPH) in rats exposed to testosterone and estradiol by Xiao-Hu Tang, Zhi-Yan Liu, Jing-Wen Ren, Heng Zhang, Ye Tian, Jian-Xin Hu, Zhao-Lin Sun, Guang-Heng Luo

“…We used RNA-seq and bioinformatics to detect differentially expressed genes (DEGs) between the prostate tissue of the novel benign prostatic hyperplasia rat group and the control group, including 458 DEGs, of which 336 were upregulated and 122 were downregulated. Then, RT-qPCR confirmed the authenticity of sequencing results. …”
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LMW Heparin Prevents Increased Kidney Expression of Proinflammatory Mediators in (NZBxNZW)F1 Mice by Annica Hedberg, Premasany Kanapathippillai, Ole Petter Rekvig, Kristin Andreassen Fenton

“…Total mRNA extracted from kidneys of heparin-treated or -untreated B/W mice was analysed by qPCR for the expression of several cytokines, chemokines, and Toll-like receptors. …”
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Ankrd1 as a potential biomarker for the transition from acute kidney injury to chronic kidney disease by Hailin Li, Lemei Hu, Changqing Zheng, Ying Kong, Ming Liang, Quhuan Li

“…The expression levels of hub genes were validated using the bulk RNA-seq data (GSE98622) and further confirmed through RT-qPCR and immunohistochemical analysis in ischemia-reperfusion injury (uIRI) mice. …”
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Increased N-Ethylmaleimide-Sensitive Factor Expression in Amygdala and Perirhinal Cortex during Habituation of Taste Neophobia by Beatriz Gómez-Chacón, Fernando Gámiz, Thomas C. Foster, Milagros Gallo

“…Total RNA was extracted and gene expression was measured by quantitative PCR (qPCR) using TaqMan gene expression assays. In addition the expression of the synaptic plasticity related immediate early genes, Homer 1 and Narp, was also assessed. …”
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Geographic Distribution of Childbirth among Adolescents in Cameroon from 2003 to 2005 by P. M. Tebeu, J. D. Kemfang, D. I. Sandjong, E. Kongnyuy, G. Halle, A. S. Doh

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Exploring Unique Extracellular Vesicles Associated Signatures: Prognostic Insights, Immune Microenvironment Dynamics, and Therapeutic Responses in Pancreatic Adenocarcinoma by Kai Nan, Ming Zhang, Zilong Geng, Yuankai Zhang, Lin Liu, Zhi Yang, Peng Xu

“…Differential expression of selected lncRNAs in both cellular and exosomal fractions of PAAD was further confirmed through quantitative polymerase chain reaction (qPCR). Eight DELs were identified from TCGA and GTEx datasets, and two exosomal lncRNAs exhibited a significant correlation with overall survival, warranting further investigation. …”
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Factors of visual organization effective on the protection of vernacular landscapes in the foothill villages by Faezeh Asadpour, Jamal-e-Din Mahdinejad, Ali Sharghi, Bahram Saleh Sedghpour

“…The techniques used in this study include content analysis, Delphi, and correlation, and the collected data are analyzed using SPSS software through Q factor analysis, Pearson correlation, and regression analysis. …”
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Evaluation of hygienic food handling practices and associated factors among food handlers in the Amhara region, Ethiopia: a systematic review and meta-analysis by Lamenew Fenta, Kebadu Tadesse

“…Eyeball testing using forest plots, Cochrane Q test statistics and I² had been used to identify and measure heterogeneity. …”
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Künstliche Intelligenz im Englischunterricht – Grundwissen und Praxisbeispiele by Inez De Florio-Hansen

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Identification of the immune infiltration and biomarkers in ulcerative colitis based on liquid–liquid phase separation-related genes by Zhixing Hong, Shilin Fang, Haihang Nie, Jingkai Zhou, Yuntian Hong, Lan Liu, Qiu Zhao

“…Both the microarray and scRNA-seq datasets showed a strong correlation with immune cell infiltration and cytokines, especially GBP1, TRIM22, SRI. And qRT-PCR analysis showed that GBP1 play a pro-inflammatory role in UC. …”
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Antecedents of Intention to Adopt Mobile Health (mHealth) Application and Its Impact on Intention to Recommend: An Evidence from Indonesian Customers by Gilbert Sterling Octavius, Ferdi Antonio

“…Mobile health developers and managers need to improve initial trust in the mHealth platform, facilitating conditions, and performance expectancy when developing the applications. With a medium Q2predict, these factors can be applied out of the research context with medium predictive power.…”
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Vascular Closure Devices versus Manual Compression in Cardiac Interventional Procedures: Systematic Review and Meta-Analysis by Naidong Pang, Jia Gao, Binghang Zhang, Min Guo, Nan Zhang, Meng Sun, Rui Wang

“…Heterogeneity among studies was evaluated by I2 index and the Cochran Q test, respectively. Publication bias was assessed using the funnel plot and Egger’s test. …”
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Transcriptome Analysis Reveals Complex Defensive Mechanisms in Salt-Tolerant and Salt-Sensitive Shrub Willow Genotypes under Salinity Stress by Dezong Sui, Baosong Wang

“…Furthermore, expressions of nine randomly selected DEGs were verified by qRT-PCR analysis. This study contributes in new perspicacity into underlying the salt tolerance mechanism of a shrub willow at the transcriptome level and also provides numerous salt-tolerant genes for further genetic engineering and breeding purposes in the future.…”
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Overexpression of microRNA-211 in Functional Dyspepsia via Downregulation of the Glial Cell Line-Derived Neurotrophic Factor (GDNF) by Increasing Phosphorylation of p38 MAPK Pathwa... by Jue Wang, Sai Gu, Bo Qin

“…Enteric glia cell (EGCs) cell line transfected with miR-211 mimics and inhibitors were used to clarify the expression levels of GDNF were analyzed by qRT-PCR and ELISA. Intestine epithelial cell (IECs) cell line cultured in medium from ECGs in different transfection conditions were used in wound healing assay, cell proliferation assay, and western blotting for evaluation of p38 MAPK phosphorylation level. …”
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Load-Bearing Characteristics of Large-Diameter Rock-Socketed Piles Based on Ultimate Load Tests by Xueying Liu, Xiaoyu Bai, Mingyi Zhang, Yonghong Wang, Songkui Sang, Nan Yan

“…The test results showed that the three test piles were damaged under maximum loading, the Q-s curve exhibited a steep drop, the pile compression was around 1.2 times the pile diameter, and the bearing capacity of a single pile did not meet the design requirements. …”
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La Norma ISO 14.063 de comunicación ambiental en la gestión de crisis organizacional by María Florencia Moya

“…Para ello se utilizó el método descriptivo, analizando las fuentes primarias (entrevistas) y secundarias (bibliografía) de información, a través del método descriptivo y la “Metodología Q” en la selección e interpretación de datos. Como resultado de la investigación se observó que en la mayoría de los casos la comunicación ambiental es poco utilizada en la planificación como medida proactiva. …”
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Three MYO15A Mutations Identified in One Chinese Family with Autosomal Recessive Nonsyndromic Hearing Loss by Fengguo Zhang, Lei Xu, Yun Xiao, Jianfeng Li, Xiaohui Bai, Haibo Wang

“…Three variants, c.3971C>A (p.A1324D), c.4011insA (p.Q1337Qfs∗22), and c.9690+1G>A, in the MYO15A gene were identified by targeted capture sequencing and Sanger sequencing, and the first two of them were novel. …”
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Antioxidant Activity In Vitro Guided Screening and Identification of Flavonoids Antioxidants in the Extract from Tetrastigma hemsleyanum Diels et Gilg by Li Ding, Xiaomin Zhang, Jiajia Zhang

“…The molecular weight of the above antioxidant components was investigated using the qualitative analytical method of high-performance liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry (HPLC-Q-TOF LC/MS). Based on the concentrations of the samples (0.2–4 mg/mL), the DPPH free radical scavenging ability, ABTS+ free radical scavenging ability, hydroxyl free radical scavenging ability, and Fe3+ reducing ability of the ethyl acetate extract (EAE) were stronger than that of the crude extract (CE), petroleum ether extract (PEE), and n-butanol extract (BE). …”
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Factors Affecting the Survival of Patients with Oligometastatic Non-Small-Cell Lung Cancer: A Meta-Analysis by Yu Shi, Jianxin Yang, Ninghua Yao, Minghai Shao, Wenxiu Ding, Wanrong Jiang, Xinchen Sun

“…Heterogeneity was analyzed using I-squared and Cochran Q tests. Furthermore, sensitivity analysis was performed to evaluate the stability of results. …”
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The Dynamic DNA Demethylation during Postnatal Neuronal Development and Neural Stem Cell Differentiation by Huikang Tao, Pei Xie, Yuhang Cao, Liqi Shu, Liping Li, Junchen Chen, Guangfeng Tian, Yingliang Zhuang, Qiang Shu, Xuekun Li

“…In this study, utilizing DNA dot blot, immunofluorescence staining, and qRT-PCR, we studied the expression pattern of Tets, the enzymes governing DNA demethylation, and the levels of 5hmC, 5fC, and 5caC during the postnatal neuronal development of mice. …”
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