Comparative Analysis of the Glutathione S-Transferase Gene Family of Four Triticeae Species and Transcriptome Analysis of GST Genes in Common Wheat Responding to Salt Stress by Yongchao Hao, Shoushen Xu, Zhongfan lyu, Hongwei Wang, Lingrang Kong, Silong Sun

“…Six GST genes which respond to different salt concentrations were selected and validated by a qRT-PCR assay. These findings will not only provide helpful information about the function of GST genes in Triticeae species but also offer insights for the future application of salt stress resistance breeding in common wheat.…”
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Effects of Different Cooling Processes on the Structure and Properties of Aluminum/Steel Composite Plate by Yufei Zhu, Runwu Jiang, Chao Yu, Yuhua Wu, Hong Xiao

“…In this paper, the Al/steel composite plate was prepared using rolling, with 5083 aluminum plate as the cladding plate, Q235 steel plate as the substrate, and TA1 titanium (Ti) plate and DT4 pure iron (Fe) plate as the intermediate layers. …”
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Microcephalic osteodysplastic primordial dwarfism type II in four Indian children with PCNT variants by Amit Singh, Mahak Garg, Mohammed Shariq, Preeti Khetarpal, Inusha Panigrahi

“…Abstract Background Microcephalic Osteodysplastic Primordial Dwarfism Type II (MOPD II) is an autosomal recessive rare genetic condition marked by characteristic clinical symptoms of prenatal and post-natal growth retardation, reduced height, and microcephaly caused by variations in PCNT gene located on chromosome 21q22. Case presentation Four patients of Indian origin with growth deficiency and additional clinical features of MOPDII were recruited from a tertiary health care center and whole exome sequencing was performed on blood samples from these patients. …”
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hsa_circ_0001599 promotes odontogenic differentiation of human dental pulp stem cells by increasing ITGA2 expression and stability by Yeqing Yang, Chong Jiang, Junkai Zeng, Xiaolan Guo, Ming Chen, Buling Wu

“…The expression of hsa_circ_0001599 in hDPSCs and dental pulp tissue was determined by using quantitative real-time PCR (qRT‒PCR). The role of hsa_circ_0001599 in the odontogenic differentiation of hDPSCs and its mechanism were studied using a variety of in vivo and in vitro assessments. …”
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Glucose Fluctuation Inhibits Nrf2 Signaling Pathway in Hippocampal Tissues and Exacerbates Cognitive Impairment in Streptozotocin-Induced Diabetic Rats by Haiyan Chi, Yujing Sun, Peng Lin, Junyu Zhou, Jinbiao Zhang, Yachao Yang, Yun Qiao, Deshan Liu

“…The protein expression levels of Nrf2, HO-1, NQO-1, Bax, Bcl-2, and Caspase-3 in the hippocampal tissues of rats were measured via Western blotting, and the mRNA expressions of Nrf2, HO-1, and NQO-1 were examined using qRT-PCR. Finally, Western blotting and immunohistochemistry were conducted to detect BDNF levels. …”
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Genome-Wide Analysis and Expression Profiling of Watermelon <i>VQ</i> Motif-Containing Genes Under Abiotic and Biotic Stresses by Yanjun He, Jia Shen, Xinyang Xu, Weisong Shou

“…Quantitative real-time PCR (qRT-PCR) analysis indicated that the majority of <i>ClVQ</i> genes were specifically or preferentially expressed in certain tissues or organs, especially in the male flower. …”
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Exploring potential biomarkers for acute myocardial infarction by combining circadian rhythm gene expression and immune cell infiltration by Xiao Yu, Xiaopeng Zhang, Hazrat Bilal, Chang Shi, Lei Sun

“…The Benjamini–Hochberg method was employed to adjust for multiple testing, and the results retained statistical significance. RT-qPCR analysis further confirmed the upregulation of these five genes under hypoxic conditions, compared to controls. …”
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Association of oxidative balance scores with cardiovascular and all cause mortality in patients with asthma by Wei Zhao, Yiyuan Sun, Bohui Zhu

“…Cardiovascular disease mortality showed consistency (Q4, HR: 0.43, 95% CI: 0.19, 0.98). The association between the OBS index and both all-cause and cardiovascular mortality in asthma patients remained stable across different models and subgroup assessments. …”
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Long noncoding RNA-EBIC promotes tumor cell invasion by binding to EZH2 and repressing E-cadherin in cervical cancer. by Ning-xia Sun, Chen Ye, Qian Zhao, Qing Zhang, Chen Xu, Shao-bing Wang, Zhi-jun Jin, Shu-han Sun, Fang Wang, Wen Li

“…The results were validated by quantitative real-time polymerase chain reaction (qPCR). Then, we found a specific differentially expressed lncRNA can physically bind to enhancer of zeste homolog2 (EZH2) by using RNA immunoprecipitation. …”
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PSA is not suitable as a reliable marker for seminal fluid in rectal samples collected post-mortem by Corine Müller, Elie Pascolo Tièche, Martin Zieger

“…The rate of positive SERATEC® PSA Semiquant tests was 33 % for women and 67 % for men. Using male specific qPCR, male DNA was detected in only one of the female samples, indicating a high false-positive rate for both sexes when using SERATEC® PSA Semiquant tests as indicators for the presence of seminal liquid post mortem. …”
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Blockade of PLD2 Ameliorates Intestinal Mucosal Inflammation of Inflammatory Bowel Disease by Guangxi Zhou, Lin Yu, Wenjing Yang, Wei Wu, Leilei Fang, Zhanju Liu

“…PLD2 expression was determined in peripheral blood cells and inflamed mucosa from patients with IBD by qRT-PCR. Colonic biopsies were also obtained from CD patients before and after infliximab (IFX) treatment to examine PLD2 expression. …”
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Selection of suitable reference genes for gene expression studies in HMC3 cell line by quantitative real-time RT-PCR by Martina Fazzina, Matteo Bergonzoni, Francesca Massenzio, Barbara Monti, Flavia Frabetti, Raffaella Casadei

“…Recently, the Human Microglial Clone 3 cell line (HMC3) has been characterized and a new human microglia model has emerged. Here we perform RT-qPCR analyses to evaluate the expression of ten reference genes in HMC3, untreated or stimulated to a pro-inflammatory status. …”
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Green chemistry approaches in the analytical validation of fosravuconazole using UV spectrophotometry and HPLC by Jeswin Maliyakal, Mital Patel

“…Both methods were rigorously validated according to ICH Q2(R1) guidelines, demonstrating their suitability for the assessment of individual substances in various mixtures. …”
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Association of TLR4-T399I Polymorphism with Chronic Obstructive Pulmonary Disease in Smokers by Matthaios Speletas, Vassiliki Merentiti, Konstantinos Kostikas, Kyriaki Liadaki, Markos Minas, Konstantinos Gourgoulianis, Anastasios E. Germenis

“…For this purpose we investigated the prevalence and any possible associations of common TLR polymorphisms (𝑇𝐿𝑅2-R753Q, 𝑇𝐿𝑅4-D299G, and 𝑇𝐿𝑅4-T399I) in a group of 240 heavy smokers (>20 pack years), without overt atherosclerosis disease, of whom 136 had developed COPD and 104 had not. …”
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circ_ZFR Is Linked to Paclitaxel Resistance in Cervical Cancer via miR-944 Sponging and IL-10 Upregulation by Xiaoqian Long, Meiyun Zheng, Youlin Yang, Yi Chen, Xiahui Zhang, Haiyan Zhang

“…The levels of IL-10 mRNA, miR-944, and circ_ZFR were measured using qRT-PCR analyses. The CCK-8 assay was used to determine PTX resistance. …”
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Cognitive analysis and path construction of Chinese students’ mathematics cognitive process based on CDA by Xiaopeng Wu, Nanxin Li, Rongxiu Wu, Hao Liu

“…With CDA techniques, this study first identified eight attributes in mathematics cognition and formed a Q-matrix based on TIMSS-2015 items to construct the diagnosis assessment. …”
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Protocol for a systematic review and meta-analysis on intention to screen for cervical cancer and predictors among women of reproductive age in Ethiopia by Begetayinoral Kussia Lahole, Tesfahun Simon, Wondimu Ewunetu, Wondafrash Kussia

“…The inverse variance (I2) and Cochran Q statistics will be used to examine the heterogeneity between studies. …”
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Establishment of Gill-Derived Primary Cell Cultures from Largemouth Bass (<i>Micropterus salmoides</i>) as an Alternative Platform for Studying Host–Virus Interactions by Ziwen Wang, Li Nie, Chenjie Fei, Jiong Chen

“…Viral replication within these primary cells was confirmed by transmission electron microscopy, and further qRT-PCR demonstrated the upregulation of antiviral genes (<i>IFN1</i>, <i>Mx1</i>, <i>ISG15</i>, and <i>Viperin</i>). …”
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Historical-pedagogical perspective of intellectual disability across the world and in Serbia by Đurić Zdravković Aleksandra A.

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Evolution of CpG-islands by means of tandem duplications by V. N. Babenko, Y. L. Orlov, Zh. T. Isakova, D. A. Antonov, M. I. Voevoda

“…We also underline the unique genomic segment, which is DXZ4 mega satellite, in q arm of chromosome X, also falling into the category of CpG-islands which evolved by tandem duplications rounds.…”
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