CD163, a novel receptor for TNF, was revealed in situ by proximity ligation assay by Alexandre Cousin, Myriam Oger, Aymar de Jenlis, Audrey Lejart, Laure Barbier, Diane Riccobono, Xavier Holy, Anne-Laure Favier, Krisztina Nikovics

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Fortilin binds CTNNA3 and protects it against phosphorylation, ubiquitination, and proteasomal degradation to guard cells against apoptosis by Mari Nakashima, Decha Pinkaew, Uttariya Pal, Fei Miyao, Hanna Huynh, Lena Tanaka, Ken Fujise

“…Using co-immunoprecipitation western blot analyses, the proximity ligation assay, microscale thermophoresis, and biolayer interferometry, we here show that fortilin specifically interacts with CTNNA3 (α-T-catenin), but not with CTNNA1, CTNNA2, or CTNNB. …”
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PTPIP51: A New Interaction Partner of the Insulin Receptor and PKA in Adipose Tissue by M. A. Bobrich, S. A. Schwabe, A. Brobeil, M. Viard, M. Kamm, F. C. Mooren, K. Krüger, C. Tag, M. Wimmer

“…Adipose tissue samples were analyzed by immunofluorescence and Duolink proximity ligation assay to quantify interactions of PTPIP51 with either insulin receptor (IR) or PKA. …”
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Adenosine A2A receptor activation is necessary to gate the TrkB-dependent intramuscular nerve sprouting during muscle reinnervation after a nerve crush by Michael Di Palma, Davide Lattanzi, Patrizia Ambrogini, Stefano Sartini

“…Immunohistochemical and in situ proximity ligation assay (isPLA) analyses were used to localize A2ARs and A2A-TrkB heteroreceptor complexes at the neuromuscular level in undenervated animals. …”
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Neuroprotection against Amyloid-β-Induced DNA Double-Strand Breaks Is Mediated by Multiple Retinoic Acid-Dependent Pathways by Julien Colas, Natacha Chessel, Allaeddine Ouared, Emmanuelle Gruz-Gibelli, Pascale Marin, François R. Herrmann, Armand Savioz

“…Furthermore, it could be shown by a proximity ligation assay that the PPARβ/δ-RXR interactions, not the RARα/β/γ-RXR interactions, increased in the cells when a 10 min RA treatment was followed by a 20 min Aβ1-42 treatment. …”
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V-ATPase in glioma stem cells: a novel metabolic vulnerability by Alessandra Maria Storaci, Irene Bertolini, Cristina Martelli, Giorgia De Turris, Nadia Mansour, Mariacristina Crosti, Maria Rosaria De Filippo, Luisa Ottobrini, Luca Valenti, Elisa Polledri, Silvia Fustinoni, Manuela Caroli, Claudia Fanizzi, Silvano Bosari, Stefano Ferrero, Giorgia Zadra, Valentina Vaira

“…Cell extracts, proximity-ligation assay and advanced microscopy was used to analyze subcellular presence of proteins. …”
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Importin subunit beta‐1 mediates ERK5 nuclear translocation, and its inhibition synergizes with ERK5 kinase inhibitors in reducing cancer cell proliferation by Zoe Lombardi, Lucia Gardini, Anatolii V. Kashchuk, Alessio Menconi, Matteo Lulli, Ignazia Tusa, Alessandro Tubita, Luisa Maresca, Barbara Stecca, Marco Capitanio, Elisabetta Rovida

“…Both co‐immunoprecipitation experiments and proximity ligation assay provided evidence that ERK5 and importin β1 interact and that this interaction is further induced by EGF administration and prevented by ivermectin treatment. …”
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Tumor CEMIP drives immune evasion of colorectal cancer via MHC-I internalization and degradation by Ying Zhu, Jiao Li, Tao Zhang, Lei Zhao, Min Jin, Jinghua Ren, Jing Tang, Haihong Wang, Zhenyu Lin, Qing Liang, Ruiqi Li, Biying Zhang, Qingling Hua, Guojie Xu, Jiayuan Chen, Lanqing Wang, Dejun Zhang, Dandan Yu

“…Protein–protein interactions were investigated by co-immunoprecipitation and proximity ligation assays. The intracellular trafficking of MHC-I was revealed by an immunofluorescent technique. …”
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Urine proteomics defines an immune checkpoint-associated nephritis signature by Cassian Yee, Jamie S Lin, James P Long, Shailbala Singh, Yanlan Dong

“…However, identification of effective biomarkers of disease using established technologies such as proximity ligation assays (PLA) and bead-based immunoassays is challenging due to their limited sensitivity and loss of precision in multiplex analysis.To address this, we employed cutting-edge NUcleic acid Linked Immuno-Sandwich Assay (NULISA) technology to measure protein expression in urine and plasma samples from AKI patients undergoing ICI therapy. …”
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