Upregulated GSDMB in Clear Cell Renal Cell Carcinoma Is Associated with Immune Infiltrates and Poor Prognosis by Yuanshan Cui, Zhongbao Zhou, Yumeng Chai, Yong Zhang

“…The Human Protein Atlas and the Clinical Proteomic Tumor Analysis Consortium (CPTAC) were used to assess the protein expression of GSDMB. …”
Get full text

Molecular mechanisms and therapeutic strategies of Naegleria fowleri Carter (1970): a review of the fatal brain-eating amoeba by N. Datta

“…With the advances in genomic and proteomic technologies, new opportunities have emerged to explore the molecular biology of N. fowleri and its host response. …”
Get full text

Phylogenetic and pangenomic analyses of members of the family <i>Micrococcaceae</i> related to a plant-growth-promoting rhizobacterium isolated from the rhizosphere of potato (<i>S... by S. Yu. Shchyogolev, G. L. Burygin, L. A. Dykman, L. Yu. Matora

“…Similar results were obtained in a full-scale AAI test against the UniProt database (about 250 million records), by using the AAI-profiler program and the proteome of K. rosea strain ATCC 186T as a query. According to the AAI &gt; 65 % criterion, members of the genus Arthrobacter and several other genera belonging to the class Actinomycetes, with a very wide geographical and ecological range of sources of isolation, should be placed into the same genus as Kocuria. …”
Get full text

MSC-sEVs exacerbate senescence by transferring bisecting GlcNAcylated GPNMB by Yihan Ma, Chongfu Zhao, Jingjing Feng, Junjie Gou, Enci Kang, Feng Guan, Qiong Wu, Xiang Li

“…We employed advanced proteomic analysis to quantify the expression levels of GPNMB in both BMMSCs and sEVs. …”
Get full text

Kaempferol protects against high-salt-induced hypertension and vascular endothelial injury by inhibiting ferroptosis through the ATF4/ACSL4 pathway by Qiuer Liang, Minghao Chen, Guangtian Chen, Pengli Xu, Lai Kwan Lam, Pengcheng Xie, Ting Xie, Wanqing Tu, Tianhao Liu, Xiaopeng Peng, Haoyao Yuan, Liguo Chen, Ya Xiao

“…Surface plasmon resonance and molecular docking analyses revealed that Kae directly binds to ATF4, while proteome microarray and co-immunoprecipitation assays demonstrated that ATF4 interacts with ACSL4 in HAECs. …”
Get full text

High interstitial fluid pressure enhances USP1-dependent KIF11 protein stability to promote hepatocellular carcinoma progression by Zhengyi Wu, Chao Li, Shouhua Zhang, Liang Sun, Junwen Hu, Bingbing Qiu, Shuiqiu Liu, Yiran Hong, Tianxiang Chen, Kai Wang, Xiangbao Yin, Jinlong Yan

“…Co-immunoprecipitation and proteomic analysis identified ubiquitin-specific peptidase 1 (USP1) as a crucial factor in this process, deubiquitinating KIF11 at the K77 site, thus stabilizing its protein levels. …”
Get full text

Immune monitoring of trabectedin therapy in refractory soft tissue sarcoma patients - the IMMUNYON study by Paulo Rodrigues-Santos, Paulo Rodrigues-Santos, Paulo Rodrigues-Santos, Paulo Rodrigues-Santos, Paulo Rodrigues-Santos, Paulo Rodrigues-Santos, Jani Sofia Almeida, Jani Sofia Almeida, Jani Sofia Almeida, Jani Sofia Almeida, Jani Sofia Almeida, Jani Sofia Almeida, Luana Madalena Sousa, Luana Madalena Sousa, Luana Madalena Sousa, Patrícia Couceiro, Patrícia Couceiro, Patrícia Couceiro, Patrícia Couceiro, António Martinho, Joana Rodrigues, Ruben Fonseca, Manuel Santos-Rosa, Manuel Santos-Rosa, Manuel Santos-Rosa, Manuel Santos-Rosa, Manuel Santos-Rosa, Paulo Freitas-Tavares, José Manuel Casanova, José Manuel Casanova, José Manuel Casanova, José Manuel Casanova, José Manuel Casanova

“…Immune monitoring included deep immunophenotyping (200+ parameters), gene expression profiling (103 genes), and soluble proteome analysis (99 analytes). Using RECIST1.1 criteria, 68.2% of patients achieved stable disease (SD), while 31.8% exhibited progression disease (PD). …”
Get full text

Bisecting GlcNAc modification of vesicular GAS6 regulates CAFs activation and breast cancer metastasis by Jing Li, Xueting Ren, Lin Cao, Kaixiang Min, Meng Wang, Shuai Lin, Lei Lei, Zengqi Tan, Xiaoliang Cheng, Xiang Li, Feng Guan

“…The effects of small extracellular vesicles (sEVs) derived from MDA-MB-231/OEvec and MDA-MB-231/OEMGAT3 cells on CAFs activation were examined using western blotting, transwell, and collagen contraction assays. Proteomic analysis was performed to identify dysregulated proteins in sEVs from different cell lines. …”
Get full text

Proximity extension assay revealed novel inflammatory biomarkers for follicular development and ovarian function: a prospective controlled study combining serum and follicular flui... by Chong Wang, Ying Feng, Yu Chen, Xianhua Lin, Xiangjuan Li

“…MethodsSerum samples from the menstruation phase (n=26), serum samples from the ovulation phase (n=26), FF samples of mature oocytes (n=26), and FF samples of immature oocytes (n=10) were collected. Olink proteomic proximity extension assay (PEA) technology was used to compare the differentially expressed proteins (DEPs), and patients were divided into two subgroups—the normal ovarian reserve (NOR) group and the DOR group—for further bioinformatics analysis and verification by enzyme-linked immunosorbent assay (ELISA).ResultsIn total, 16 DEPs were detected between the mature group and the immature group (FF), and 11 DEPs were detected between the ovulation group and the menstruation group (serum). …”
Get full text

Disturbed shear stress promotes atherosclerosis through TRIM21‐regulated MAPK6 degradation and consequent endothelial inflammation by Feng Wang, Shu‐Yu Wang, Yue Gu, Shuai Luo, Ai‐Qun Chen, Chao‐Hua Kong, Wen‐Ying Zhou, Li‐Guo Wang, Zhi‐Mei Wang, Guang‐Feng Zuo, Xiao‐Fei Gao, Jun‐Jie Zhang, Shao‐Liang Chen

“…Methods In vitro and in vivo experiments, RNA‐seq, CO‐IP and proteomic analysis, combined with single‐cell RNA‐seq datasets were used to reveal the upstream and downstream mechanisms involved. …”
Get full text

Targeting MYCN upregulates L1CAM tumor antigen in MYCN-dysregulated neuroblastoma to increase CAR T cell efficacy by Laura Grunewald, Lena Andersch, Konstantin Helmsauer, Silke Schwiebert, Anika Klaus, Anton G. Henssen, Teresa Straka, Marco Lodrini, Sebastian G. Wicha, Steffen Fuchs, Falk Hertwig, Frank Westermann, Alice Vitali, Carlotta Caramel, Gabriele Büchel, Martin Eilers, Kathy Astrahantseff, Angelika Eggert, Uta E. Höpken, Johannes H. Schulte, Thomas Blankenstein, Kathleen Anders, Annette Künkele

“…RNA sequencing datasets characterizing the model were compared to publicly available RNA/proteomic datasets. MYCN-directed L1CAM regulation was explored using public ChIP-sequencing datasets. …”
Get full text

Lipoxin A4 yields an electrophilic 15-oxo metabolite that mediates FPR2 receptor-independent anti-inflammatory signaling by Adolf Koudelka, Gregory J. Buchan, Veronika Cechova, James P. O’Brien, Emily R. Stevenson, Crystal E. Uvalle, Heng Liu, Steven R. Woodcock, Steven J. Mullett, Cheng Zhang, Bruce A. Freeman, Stacy L. Gelhaus

“…Rather, if present in sufficient concentrations, LXA4 and other mono- and poly-hydroxylated unsaturated fatty acids synthesized by macrophages would be readily oxidized to electrophilic α,β-unsaturated ketone products that modulate the redox-sensitive cysteine proteome via G-protein coupled receptor-independent mechanisms.…”
Get full text

Bioelectric stimulation outperforms brain derived neurotrophic factor in promoting neuronal maturation by María del Pilar Diego-Santiago, María Ujué González, Esther María Zamora Sánchez, Nuria Cortes-Carrillo, Carlos Dotti, Francesc Xavier Guix, Sahba Mobini

“…ES with large enough accumulated charge significantly enriched EV proteome associated with neural development and function. …”
Get full text

Characterization and genome analysis of lytic Vibrio phage VPK8 with potential in lysing Vibrio parahaemolyticus isolates from clinical and seafood sources by Valalak Jintasakul, Jiranan Pattano, Sutima Preeprem, Pimonsri Mittraparp-arthorn

“…Phylogenetic analysis grouped it within the Autographiviridae family, showing 95.96% similarity to Vibrio phage vB_VpaP_MGD1. Viral proteomic analysis placed VPK8 within the Pseudomonadota host group. …”
Get full text

Exploring MAP3K genes in gastric cancer: biomarkers, tumor microenvironment dynamics, and chemotherapy resistance by Senhui Wei, Ying Li, Jing Zhou, Yongming Xia

“…Furthermore, the diagnostic potential was demonstrated through robust Receiver operating characteristics (ROC) curve analysis, indicating the strong discriminatory power of these genes in distinguishing GC patients. Proteomic analysis further confirmed the higher expression of MAP3K1, MAP3K4, MAP3K7, MAP3K8, MAP3K9, and MAP3K10 genes in GC. …”
Get full text

Palaeoproteomic identification of a whale bone tool from Bronze Age Heiloo, the Netherlands by Dekker, Joannes A. A., Mylopotamitaki, Dorothea, Verbaas, Annemieke, Sinet-Mathiot, Virginie, Presslee, Samantha, McCarthy, Morgan L., Olsen, Morten Tange, Olsen, Jesper V., van den Hurk, Youri, Brattinga, Joris, Welker, Frido

Get full text

Metabolic and molecular Characterization, following dietary exposure to DINCH, Reveals new Implications for its role as a Metabolism-Disrupting chemical by Sontje Krupka, Alix Sarah Aldehoff, Cornelius Goerdeler, Beatrice Engelmann, Ulrike Rolle-Kampczyk, Kristin Schubert, Nora Klöting, Martin von Bergen, Matthias Blüher

“…Female mice exhibited impaired whole-body insulin sensitivity and higher triglyceride levels, while male mice showed an altered insulin/C-peptide ratio and elevated cholesterol, HDL, and LDL levels. Proteomic profiling of serum, adipose and liver tissues revealed changes in pathways related to central energy metabolism and immune response, highlighting the systemic impact of DINCH, potentially on inflammatory processes. …”
Get full text

Clinical-grade extracellular vesicles derived from umbilical cord mesenchymal stromal cells: preclinical development and first-in-human intra-articular validation as therapeutics f... by Aliosha I. Figueroa-Valdés, Patricia Luz-Crawford, Yeimi Herrera-Luna, Nicolás Georges-Calderón, Cynthia García, Hugo E. Tobar, María Jesús Araya, José Matas, Darío Donoso-Meneses, Catalina de la Fuente, Jimena Cuenca, Eliseo Parra, Fernando Lillo, Cristóbal Varela, María Ignacia Cádiz, Rolando Vernal, Alexander Ortloff, Gino Nardocci, Verónica Castañeda, Catalina Adasme-Vidal, Maximiliano Kunze-Küllmer, Yessia Hidalgo, Francisco Espinoza, Maroun Khoury, Francisca Alcayaga-Miranda

“…In silico studies confirmed sEV's immunosuppressive signature through miRNA and proteome analysis. In an OA mouse model, sEV injected intra-articularly (IA) induced hyaline cartilage regeneration, validated by histological and μCT analyses. …”
Get full text

THE SOLANUM TUBEROSUM KNOWLEDGE BASE: THE SECTION ON MOLECULAR-GENETIC REGULATION OF METABOLIC PATHWAYS by T. V. Ivanisenko, O. V. Saik, P. S. Demenkov, V. K. Khlestkin, E. K. Khlestkina, N A. Kolchanov, V. А. Ivanisenko

“…Rapid development of high-performance genomic, transcriptomic, proteomic and metabolic technologies led to an information explosion in the field of plant biology and agrobiology. …”
Get full text

Extracellular vesicles (EVs) in seminal plasma of buffalo bulls possess spermatozoa specific fertility associated proteins and enzymes by Rakesh Kumar, Mir Ahmad, Bala Subramaniam, Ankit Pal, Aditya Patel, Shiva Shiva, Vitika Chauhan, Seema Karanwal, Mukesh Bhakat, Tirtha K. Datta

“…Finally, LC-MS/MS-based high throughput proteome was performed to identify the differential abundance of protein cargo in seminal EVs of contrasting fertility bulls. …”
Get full text