Adipose-Derived Mesenchymal Stem Cells Enhance Ovarian Cancer Growth and Metastasis by Increasing Thymosin Beta 4X-Linked Expression by Yijing Chu, Min You, Jingjing Zhang, Guoqiang Gao, Rendong Han, Wenqiang Luo, Tingting Liu, Jianxin Zuo, Fuling Wang

“…However, the underlying mechanism has not yet been revealed. In this study, a proteomics analysis was performed to compare protein expression treated with and without ADSCs in ovarian cancer cells. …”
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Anti-colon cancer activity of water-soluble polysaccharides extracted from Gloeostereum incarnatum via Wnt/β-catenin signaling pathway by Jiawei He, Anhui Yang, Xuyu Zhao, Yang Liu, Shuyan Liu, Di Wang

“…Eight-week administration with GIPS at doses of 30 or 90 mg/kg strongly inhibited tumor growth including the reduction on numbers and the suppression of the size without influencing the animals’ body weight and organ functions. According to the proteomics performing by antibody array, among 308 detected cytokines, GIPS significantly regulated 89 of them. …”
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Cucurbitacin IIa Alleviates Colitis via Promoting the Release of Host-Derived Extracellular Vesicles Encapsulating microRNA-30b-5p by Zhao Y, Jiang B, Zuo S

“…EVs were isolated and characterized, followed by analysis of the small RNAs and proteins encapsulated within them using small RNA sequencing and proteomics, respectively.Results: Our results revealed that cucurbitacin IIa alleviated colitis symptoms in DSS-treated rats, along with changes in the morphology and composition of intestinal EVs. …”
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Activity of Various Cathepsin Proteases and Enrichment of Klotho Protein in the Urine and Urinary Extracellular Vesicles After SARS-CoV-2 Infection by Niharika Bala, Ramish H. Rafay, Sarah C. Glover, Abdel A. Alli

“…Urinary extracellular vesicles (uEVs) were isolated from urine samples from the two groups and used for proteomic analysis and subsequent pathway analyses. …”
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A multi-omics spatial framework for host-microbiome dissection within the intestinal tissue microenvironment by Bokai Zhu, Yunhao Bai, Yao Yu Yeo, Xiaowei Lu, Xavier Rovira-Clavé, Han Chen, Jason Yeung, Dingani Nkosi, Jonathan Glickman, Antonio Delgado-Gonzalez, Georg K. Gerber, Mike Angelo, Alex K. Shalek, Garry P. Nolan, Sizun Jiang

“…We demonstrate MicroCart by investigating gut host and microbiome changes in a murine colitis model, using spatial proteomics, transcriptomics, and glycomics. Our findings reveal a global but systematic transformation in tissue immune responses, encompassing tissue-level remodeling in response to host immune and epithelial cell state perturbations, bacterial population shifts, localized inflammatory responses, and metabolic process alterations during colitis. …”
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Detection of chimeric alpha-defensin transcripts and peptides in mouse Paneth cells by Steven Timmermans, Steven Timmermans, Charlotte Wallaeys, Charlotte Wallaeys, Somara De Beul, Somara De Beul, Natalia Garcia-Gonzales, Natalia Garcia-Gonzales, Claude Libert, Claude Libert

“…IntroductionIn mammals, Paneth cells, located in the crypts of the small intestine, produceantimicrobial peptides that serve to keep the intestinal microbiome under control. a-Defensins are the primary antimicrobial peptides produced by these cells.MethodsWe used 148 publicly available bulk RNA-seq samples on purified PCs, proteomics on enriched purified PC proteins and Defa peptide activity assays to detect all Defa transcrips, including potential chimeric transcrips.ResultsWe identified 28 expressed Defa genes in mice, with up to 85% of Paneth cell RNA reads mapping to these genes. …”
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Impact of High Glucose on Bone Collagenous Matrix Composition, Structure, and Organization: An Integrative Analysis Using an Ex Vivo Model by Rita Araújo, Ricardo N. M. J. Páscoa, Raquel Bernardino, Pedro S. Gomes

“…A comprehensive approach integrating histological evaluation, histomorphometry, ATR-FTIR spectroscopy, and proteomics was adopted to unravel structural, biochemical, and molecular changes in the ECM. …”
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Anaesthetic Efficacy of 4% Articaine in Comparison with 2% Lidocaine as Intraligamentary Injections after an Ineffective Inferior Alveolar Nerve Block in Mandibular Molars with Irr... by Nazanin Zargar, Elnaz Shooshtari, Leila Pourmusavi, Alireza Akbarzadeh Baghban, Hengameh Ashraf, Ardavan Parhizkar

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Potential biomarkers for predicting the risk of thyroid cancer in immunosenescence: a population-based and externally validated multi omics study by Qian Li, Yuanyuan Zhao, Jiawei Yan, Chao He

“…Subsequently, we conducted a meta-analysis on the relationship between Hannum DNA methylation age and thyroid cancer risk, which identified 138 potential risk loci through FUMA. Additionally, proteomics and transcriptomics collectively identified 6 potential targets related to immunosenescence and thyroid cancer. …”
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Potential Pathways Involved in Elaidic Acid Induced Atherosclerosis in Human Umbilical Vein Endothelial Cells by Huahong Yu, Xiangmei Li, Zhongshang Liang, Bin Qiu, Siguang Li, Ting Luo, Jing Li, Hongyan Li, Zeyuan Deng

“…In the presented study, two-dimensional electrophoresis based proteomics was used to discover the role of elaidic acid in atherosclerosis. …”
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Exploring the Mesenchymal Stem Cell Secretome for Corneal Endothelial Proliferation by Bert Van den Bogerd, Nadia Zakaria, Steffi Matthyssen, Carina Koppen, Sorcha Ní Dhubhghaill

“…Specific identification of the underlying proteins using proteomics could render a bioactive protein that can be used for ex vivo expansion of cells or as an in vivo drug to treat early corneal endothelial damage.…”
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A ribosome-associating chaperone mediates GTP-driven vectorial folding of nascent eEF1A by Ibrahim M. Sabbarini, Dvir Reif, Kibum Park, Alexander J. McQuown, Anjali R. Nelliat, Charlotte Trejtnar, Volker Dötsch, Eugene I. Shakhnovich, Andrew W. Murray, Vladimir Denic

“…Here, we use AlphaPulldown to identify Ypl225w (also known as Chp1, Chaperone 1 for eEF1A) as a conserved yeast protein predicted to stabilize the N-terminal, GTP-binding (G) domain of eEF1A against its misfolding propensity, as predicted by computational simulations and validated by microscopy analysis of ypl225wΔ cells. Proteomics and biochemical reconstitution reveal that Ypl225w functions as a co-translational chaperone by forming dual interactions with the eEF1A G domain nascent chain and the UBA domain of ribosome-bound nascent polypeptide-associated complex (NAC). …”
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Allergen degradation of bee pollen by lactic acid bacteria fermentation and its alleviatory effects on allergic reactions in BALB/c mice by Shuting Yin, Qiangqiang Li, Yuxiao Tao, Enning Zhou, Kun Wang, Wanwen Chen, Xiangxin Li, Liming Wu

“…Four novel allergens (glutaredoxin, oleosin-B2, catalase and lipase) were identified with significant decreases in LAB-fermented BP (FBP) than natural BP by proteomics. Meanwhile, metabolomics analysis showed significant increases of 28 characteristic oligopeptides and amino acids in FBP versus BP, indicating the degradation of LAB on allergens. …”
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Synthesis of Alkyl α-Amino-benzylphosphinates by the Aza-Pudovik Reaction; The Preparation of the Butyl Phenyl-<i>H</i>-phosphinate Starting P-Reagent by Bence Bajusz, Dorka Nagy, Regina Tóth, Zsuzsanna Szalai, Ágnes Gömöry, Angéla Takács, László Kőhidai, György Keglevich

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Non-histone lactylation: unveiling its functional significance by Pusong Shi, Yongjie Ma, Yongjie Ma, Yongjie Ma, Yongjie Ma, Yongjie Ma, Shaolu Zhang, Shaolu Zhang, Shaolu Zhang, Shaolu Zhang, Shaolu Zhang

“…Recently, high-throughput proteomics studies have identified a large number of lactylated proteins and sites, revealing their global regulatory role in disease development. …”
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The Synthesis, Crystal Structure, Modification, and Cytotoxic Activity of α-Hydroxy-Alkylphosphonates by Zsuzsanna Szalai, Anna Sára Kis, Angéla Takács, László Kőhidai, Konstantin Karaghiosoff, Mátyás Czugler, László Drahos, György Keglevich

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Investigating the role of intratumoral Streptococcus mitis in gastric cancer progression: insights into tumor microenvironment by Ping Yang, Gaoli Liang, Yangyue Ni, Xiaojie Chu, Xiaoshan Zhang, Zhongyu Wang, Adeel Khan, Fangfang Jin, Han Shen, Miao Li, Zhipeng Xu

“…Here, using 16 S rRNA sequencing, tumor tissue proteomics and serum cytokines analysis, we identified enrichment of specific microbial communities within tumors of gastric cancer patients, possibly affecting the tumor microenvironment by immune modulation, metabolic processes, and inflammatory responses. …”
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A graph neural network approach for hierarchical mapping of breast cancer protein communities by Xiao Zhang, Qian Liu

“…Results Using a large amount of publicly available proteomics data, we created a hierarchical tree for breast cancer protein communities using a novel hierarchical graph neural network, with the supervision of gene ontology terms and assistance of a pre-trained deep contextual language model. …”
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Sensory Diet Program Through In-Person and Tele-Occupationa Therapy in Children with Autism Spectrum Disorder: A Single-Blind Randomized Controlled Trial by Simin Dehghani, Marzieh Pashmdarfard, Zahra Pashazadeh Azari, Alireza Akbarzadeh Baghban, Sima Dehghani, Navid Mirzakhani Araghi

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An Innovative Method to Identify Autoantigens Expressed on the Endothelial Cell Surface: Serological Identification System for Autoantigens Using a Retroviral Vector and Flow Cytom... by Tsuyoshi Shirai, Hiroshi Fujii, Masao Ono, Ryu Watanabe, Tomonori Ishii, Hideo Harigae

“…For identification of autoantigens, proteomics and expression library analyses have been performed for many years with some success. …”
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