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    Ganciclovir Resistance-Linked Mutations in the HCMV <i>UL97</i> Gene: Sanger Sequencing Analysis in Samples from Transplant Recipients at a Tertiary Hospital in Southern Brazil by Anna Caroline Avila da Rocha, Grazielle Motta Rodrigues, Alessandra Helena da Silva Hellwig, Dariane Castro Pereira, Fabiana Caroline Zempulski Volpato, Afonso Luís Barth, Fernanda de-Paris

    Published 2025-01-01
    “…This study aimed to optimize a Sanger sequencing protocol for analyzing GCV resistance-linked mutations in the HCMV <i>UL97</i> gene from plasma samples of transplant patients treated at Hospital de Clínicas de Porto Alegre, Rio Grande do Sul, Brazil. <b>Methods:</b> A nested-PCR approach combined with a touchdown PCR method was employed to enhance the sensitivity and specificity of the sequencing analysis. …”
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