Analysis of bacterial genetic diversity in biofloc by using ARDRA 16S-rRNA gene by , Widanarni, Dewi Nurhayati, Dinamella Wahjuningrum

“…ABSTRACT   This study aimed to analyze the genetic diversity of bacteria associated in bioflocs using 16S-rRNA polymerase chain reaction (PCR) with ARDRA technique. A total of 38 dominant bacterial isolates was obtained from bioflocs samples and of these isolates, 16S-rRNA gene was then isolated and amplified using PCR. …”
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Varicella Zoster Virus-induced Acute Retinal Necrosis Following Acute Meningoencephalitis in a Patient with Presumed COVID-19 by Kiana Hassanpour, Faezeh Khorasanizadeh, Mahmood Nabavi, Narsis Daftarian, Alireza Ramezani

“…Magnetic resonance imaging (MRI), cerebrospinal fluid (CSF) analysis, polymerase chain reaction (PCR) of the aqueous humor, reverse transcription polymerase chain reaction (RTPCR) of the nasopharyngeal swab specimen, chest computed tomography (CT), and fundus photography were performed for the patient. …”
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Development and validation of highly sensitive multiplex real-time RT-PCR assay for detection of classical swine fever virus genome by A. S. Sadchikova, A. S. Igolkin, R. S. Chernyshev, A. A. Kozlov, I. S. Kolbin, A. V. Sprygin, D. A. Biryuchenkov, I. A. Chvala, A. Mazloum

“…Therefore, areal-time reverse transcription-polymerase chain reaction using an internal control sample has been developed. …”
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Effect of decitabine on PD-L2 methylation in whole blood of iodine-induced autoimmune thyroiditis rats by Baiming Jin, Yanbo Qi, Hong Chao, Xiaolei Yang, Hongjie Li, Siyuan Wan

“…After injecting Dec intraperitoneally into EAT rats, we performed arsenic-cerium catalytic spectrophotometry, pathological hematoxylin and eosin staining, enzyme-linked immunosorbent assay, quantitative methylation-specific polymerase chain reaction (qMSP), and quantitative real-time polymerase chain reaction (qPCR) to determine the relevant indices. …”
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Isolation, identification and phenotypic characterization of lactococcus garvieae from rainbow trout (onchyrnus mykiss) fisheries in konya region by Kürşat Kav, Osman Erganiş

“…API 20 Strep, polymerase chain reaction (PCR) and classical bacteriological techniques were used the identification of agents. …”
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Identification of rare atypical BCR-ABL1 transcript: A case report by Jayesh Saha, Vipin Gopinath, Chandran K. Nair, Deepak Roshan

“…This case highlights the pitfalls of using real-time quantitative reverse-transcription polymerase chain reaction (RQ-PCR) for diagnosis of CML, especially when the clinical picture and the test results are discordant.…”
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Successful rivaroxaban and doxycycline therapy for Mycoplasma pneumonia with pulmonary embolism in children: a case report by Fang Xiaoqian, Lu Hemin

“…M pneumoniae polymerase chain reaction of bronchoalveolar lavage fluid was positive, and computed tomography angiography indicated pulmonary embolism. …”
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Laboratory Diagnosis of Chlamydia Pneumoniae Infections by Rosanna W Peeling

“…Antigen detection tests such as enzyme immunoassay and direct fluorescent antibody assay, and molecular detection methods such as the polymerase chain reaction assay, may provide a rapid diagnosis without the requirement for stringent transport conditions. …”
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The biological control agent for bacterial canker of kiwifruit, in Aureo® Gold, is a strain of Aureobasidium pullulans identifiable by novel SCAR marker primers by Deirdre A. Cornish, Magan M. Schipper, Jenny M. Oldham, Janet Yu, Joel L. Vanneste

“…To prevent the detection of false negatives, we developed a duplex polymerase chain reaction (PCR) based on those primers and primers which amplify a segment of the ITS. …”
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Molecular genetic and bacteriological methods of bovine mycoplasmosis diagnosis by E. V. Remizova, A. V. Gorbatov, L. K. Semina, Z. A. Skulyabina, N. V. Shmidt, G. A. Baldicheva, N. N. Avduevskaya

“…The paper presents data on mycoplasma infection diagnosis in cattle and results of differentiation of isolated mycoplasma and L-form bacteria colonies using multiple passaging and real-time polymerase chain reaction. For that, 177 samples were collected from animals with mycoplasmosis clinical signs, 45 of them were tested using molecular genetic method, 132 samples were subjected to bacteriological testing. …”
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Molecular Evidence of Coinfection with Acute Respiratory Viruses and High Prevalence of SARS-CoV-2 among Patients Presenting Flu-Like Illness in Bukavu City, Democratic Republic of... by Patrick Bisimwa Ntagereka, Rodrigue Ayagirwe Basengere, Tshass Chasinga Baharanyi, Théophile Mitima Kashosi, Jean-Paul Chikwanine Buhendwa, Parvine Basimane Bisimwa, Aline Byabene Kusinza, Yannick Mugumaarhahama, Dieudonne Wasso Shukuru, Simon Baenyi Patrick, Ronald Tonui, Ahadi Bwihangane Birindwa, Denis Mukwege

“…Nasopharyngeal swab specimens were collected to detect SARS-CoV-2 using real-time reverse transcription-polymerase chain reaction (RT-PCR), and 10 common respiratory viruses were detected by multiplex reverse transcription-polymerase chain reaction assay. …”
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Molecular Epidemiology of SARS-CoV-2 within Accra Metropolis Postlockdown by Frank T. Aboagye, Lawrence Annison, Henry Kwadwo Hackman, Maame E. Acquah, Yvonne Ashong, Isaac Owusu-Frimpong, Bill C. Egyam, Sharon Annison, George Osei-Adjei, Samuel Antwi-Baffour

“…Samples were subjected to nucleic acid extraction and followed by real-time polymerase chain reaction for the detection and quantification of SARS-CoV-2 RNA. …”
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Emergence of Enteric Viruses in Production Chickens Is a Concern for Avian Health by Elena Mettifogo, Luis F. N. Nuñez, Jorge L. Chacón, Silvana H. Santander Parra, Claudete S. Astolfi-Ferreira, José A. Jerez, Richard C. Jones, Antonio J. Piantino Ferreira

“…Chicken astrovirus (CAstV), rotavirus, reovirus, chicken parvovirus (ChPV), fowl adenovirus of subgroup I (FAdV-1), and avian nephritis virus (ANV) were investigated using the conventional polymerase chain reaction (PCR) and the reverse transcription polymerase chain reaction (RT-PCR). …”
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Lack of Tissue Virological Response as a Predictor of Relapse in Chronic Hepatitis D after Completion Bulevirtide Therapy by P. O. Bogomolov, N. A. Shub, N. A. Gasilova, A. A. Belyakova, T. G. Lidzhieva, E. A. Kondrasheva, N. A. Barsukova, M. V. Arapova, E. A. Isaeva, S. V. Koblov, M. V. Kalashnikov, O. S. Kuzymina, A. Yu. Demyanov, A. O. Bueverov, I. V. Maev

“…Aim: to evaluate the significance of a positive polymerase chain reaction result for hepatitis D virus RNA (HDV RNA) in liver biopsy specimens of patients with chronic hepatitis D (CHD) after completion of antiviral therapy (AVT) as a predictor of infection relapse.Materials and methods. …”
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Infarct‐like myocarditis with coronary vasculitis and aneurysm formation caused by Epstein–Barr virus infection by Cristina Chimenti, Romina Verardo, Claudia Grande, Marco Francone, Andrea Frustaci

“…Two additional frozen samples were evaluated by real‐time polymerase chain reaction for the presence of cardiotropic viral genomes. …”
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Glucometabolic-Related Genes as Diagnostic Biomarkers and Therapeutic Targets for Alzheimer’s Disease and Type 2 Diabetes Mellitus: A Bioinformatics Analysis by Shuo Liu, He Chen, Xiao-Dong He, Xiao-Ou Yang

“…Key genes were further validated using quantitative real-time polymerase chain reaction (qRT-PCR) in AD and T2DM murine models. …”
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Reconstruction of Alzheimer’s Disease Cell Model In Vitro via Extracted Peripheral Blood Molecular Cells from a Sporadic Patient by Sijun Liu, Yuying Zhao, Xiaoying Su, Chengcheng Zhou, Peifen Yang, Qiusan Lin, Shijun Li, Hanxu Tan, Qi Wang, Changjun Wang, Qingguang Wu

“…Immunofluorescence staining and quantitative real-time polymerase chain reaction (qPCR) suggested that PBMC-iPSCs were successfully differentiated into neural cells. …”
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COVID-19 Infection Negative in Nasopharyngeal Swabs but Suspected in Computed Tomography and Confirmed in Bronchoalveolar Lavage Material by Robert Chrzan, Tadeusz Popiela, Maciej Małecki, Jan Skupień, Amira Bryll, Anna Grochowska

“…We present a case of a patient with clinical symptoms of pneumonia, negative in several polymerase chain reaction COVID-19 tests from nasopharyngeal swabs but suspected in computed tomography and finally confirmed in bronchoalveolar lavage material.…”
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Dual bombard by Cytomegalovirus and Mycobacterium tuberculosis in a renal transplantee by Cinthujah Balachander, Indira Ananthapadmanabasamy, Ramasubramanian Viswanathan, Poongodi Santhanakumarasamy

“…By Cytomegalovirus DNA polymerase chain reaction, 9360 copies were detected in plasma. …”
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Isolated Splenic Cold Abscesses with Perisplenic Extension: Treated Successfully without Splenectomy by Mohan Khadka, Ravi Pradhan

“…Ultrasound-guided aspiration of the abscess fluid revealed Mycobacterium tuberculosis (MTB) by polymerase chain reaction (PCR). No primary focus of the infection was detected in the lungs or any other organs. …”
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