Melatonin Reverses the Loss of Stemness Induced by TNF-α in Human Bone Marrow Mesenchymal Stem Cells through Upregulation of YAP Expression by Xudong Wang, Tongzhou Liang, Jincheng Qiu, Xianjian Qiu, Bo Gao, Wenjie Gao, Chengjie Lian, Taiqiu Chen, Yuanxin Zhu, Anjing Liang, Peiqiang Su, Yan Peng, Dongsheng Huang

“…The results of colony formation assays, Alizarin red staining, western blotting, and reverse transcription-polymerase chain reactions suggest that melatonin can reverse the inflammatory damage caused by TNF-α treatment in the third, seventh, and tenth generations of primary BMMSCs (vs. control and the TNF-α-treated group). …”
Get full text

Molecular Assessment of Domain I of Apical Membrane Antigen I Gene in Plasmodium falciparum: Implications in Plasmodium Invasion, Taxonomy, Vaccine Development, and Drug Discovery by Che Roland Achungu, Damian Nota Anong, Robert Adamu Shey, Cevie Jesenta Tabe

“…Domain I of the Plasmodium falciparum AMA1 gene was amplified using nested polymerase chain reactions, and the amplified products were removed, purified, and sequenced. …”
Get full text

Mosquito‐derived ingested DNA as a tool for monitoring terrestrial vertebrates within a peri‐urban environment by Christine Chivas, Adam Stow, Andrew Harford, Thomas J. Mooney, David Loewensteiner, Kate Montgomery, Anthony Chariton

“…DNA was extracted from individual mosquitoes and amplified using four different primers, targeting vertebrates, mammals, and birds, with and without a human blocking primer (except for the bird polymerase chain reactions). The overall diversity retrieved reveals a broad diversity of species with 10 avian taxa and six mammalian taxa, including both native and non‐native species of varying body sizes and behavioral characteristics. …”
Get full text

Molecular Epidemiology and Antimicrobial Resistance of Extended-Spectrum β-Lactamase (ESBL)-Producing Klebsiella pneumoniae in Retail Cattle Meat by Nasrin Akter Liza, Hemayet Hossain, Md. Shahidur Rahman Chowdhury, Jarin Al Naser, Rayhan Mahmud Lasker, Asikur Rahman, Md. Ariful Haque, Md. Al Mamun, Md. Mukter Hossain, Md. Mahfujur Rahman

“…The bacterial isolates were obtained through biochemical and cultural techniques, and the identification of K. pneumoniae was accomplished using polymerase chain reactions (PCRs). Antimicrobial susceptibilities were assessed using disk diffusion in accordance with the Clinical and Laboratory Standards Institute (CLSI, 2020) guidelines. …”
Get full text

Deciphering the regulatory programs of RNA binding proteins in rheumatoid arthritis through single-cell transcriptome analysis by Hongbin Luo, Qunya Zheng, Youzheng Zhou, Weipeng Lai, Nanwen Zhang, Peng Chen

“…Quantitative polymerase chain reactions (PCRs) were performed to confirm differentially expressed RBPs in RA fibroblast-like synoviocytes (FLSs) and OA-FLSs, as well as in mice with collagen-induced arthritis (CIA) and control mice. …”
Get full text

The Protective Effects of Maresin 1 in the OVA-Induced Asthma Mouse Model by Guochun Ou, Qin Liu, Chengxiu Yu, Xiaoju Chen, Wenbo Zhang, Yong Chen, Tao Wang, Yongqiang Luo, Guolu Jiang, Mingmei Zhu, Hongmei Li, Mei Zeng

“…Quantitative real-time polymerase chain reactions (qRT-PCRs) were used to evaluate the expression levels of COX-2 and ICAM-1 in lung tissues. …”
Get full text

Technologies of suspension cultivation in bioreactor of the Chlamydophila abortus strain on McCoy cell culture by Yerkin Krykbayev, Nurtas Tyrsynbayev, Nurlan Akhmetsadykov, Damir Khussainov, Zhanat Batanova

“…Unfortunately, in practice, the use of the cultural diagnostic method is associated with a time-delayed result, which is a disadvantage of this diagnostic method compared to the use of polymerase chain reaction…”
Get full text

The Class III PI3K/Beclin-1 Autophagic Pathway Participates in the mmLDL-Induced Upregulation of ETA Receptor in Mouse Mesenteric Arteries by Xi Xie, Chen Chen, Cang-Bao Xu, Jie Lin, Lei Cao, Gen Chen, Jie Li

“…The dose-effect curve of endothelin-1- (ET-1-) induced mesenteric artery contraction was measured using myography, while ETA receptor mRNA expression was detected using real-time polymerase chain reactions, and the protein levels of the ETA receptor, class III PI3K, Beclin-1, LC3 II/I, p62, NF-κB, and p-NF-κB were observed using Western blot analysis. mmLDL significantly strengthened ET-1-induced contraction (the Emax value increased from 184.87 ± 7.46% in the NS group to 319.91 ± 20.31% in the mmLDL group (P<0.001), and the pEC50 value increased from 8.05 ± 0.05 to 9.11 ± 0.09 (P<0.01). …”
Get full text

Polymorphisms and dental age in non-syndromic cleft lip and palate: a cross-sectional study by Gabriela Fonseca-Souza, Lhorrany Alves-Souza, Maria Angélica Hueb de Menezes-Oliveira, Nikolaos Daratsianos, Svenja Beisel-Memmert, Christian Kirschneck, Rafaela Scariot, Juliana Feltrin-Souza, Erika Calvano Küchler

“…Genomic DNA was extracted from buccal cells, and SNPs in Epidermal Growth Factor (EGF) – rs4444903 and rs2237051, Epidermal Growth Factor Receptor (EGFR) – rs2227983 –, Transforming Growth Factor Beta 1 (TGFB1) – rs1800470 and rs4803455 –, and Transforming Growth Factor Beta Receptor 2 (TGFBR2) – rs3087465 – were genotyped by real-time polymerase chain reactions using the TaqMan assay. The Student T-test was used to compare the variations in DA between the phenotypes “with CL ± P” and “without CL ± P”, and the ANOVA two-way test was performed to compare the variations in DA among the genotypes (α = 0.05). …”
Get full text