Promotion of microfracture-mediated cartilage repair by the intra-articular injection of Mg2+ by Zhian Chen, Tianhua Zhou, Zhengbo Yin, Peiya Duan, Ying Zhang, Yujiao Feng, Rongmao Shi, Yongqing Xu, Rongqing Pang, Hongbo Tan

“…The expression levels of related genes were detected by real-time quantitative reverse transcription polymerase chain reaction (RT-qPCR). Results: The histological results showed that the 0.5 mol/L Mg2+ group had deeper positive staining in haematoxylin-eosin (H&E), safranin O, Alcian blue, and type II collagen staining. …”
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Low Expression of FFAR2 in Peripheral White Blood Cells May Be a Genetic Marker for Early Diagnosis of Acute Myocardial Infarction by Jianjun Ruan, Heyu Meng, Xue Wang, Weiwei Chen, Xiaomin Tian, Fanbo Meng

“…Real-time fluorescence quantitative polymerase chain reaction was used to detect the expression of the FFAR2 gene. …”
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Association of rs3755319 and rs4148325 of the <i>UGT1A1</i> gene, rs2328136 of the <i>NUP153-AS</i> gene, and rs16928809 of the <i>SLC22A18</i> gene with benign unconjugated hyperb... by Anastasiya A. Ivanova, Natalia E. Apartseva, Anastasiia P. Kashirina, Elena G. Nemcova, Julija V. Ivanova, Margarita V. Kruchinina, Svetlana A. Kurilovich, Vladimir N. Maksimov

“…Genotyping of the groups by rs3755319, rs4148325 of the UGT1A1 gene, rs2328136 of the NUP153-AS gene, and rs16928809 of the SLC22A18 gene was performed by polymerase chain reaction followed by restriction fragment length polymorphism analysis. …”
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ZC3H15 suppression ameliorates bone cancer pain through inhibiting neuronal oxidative stress and microglial inflammation by Li-Quan Huang, Ting-Xuan Yan, Bao-Sheng Wang, Hao Li, Nai-Bao Zhou

“…Methods: To evaluate the expression of ZC3H15 in cancer-induced pain, we used microcomputed tomography (MicroCT), immunoblotting, co-immunoprecipitation (Co-IP), behavior tests, quantitative real-time polymerase chain reaction (qRT-PCR), and immunofluorescence assays in this investigation. …”
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Impact of periodontal microRNAs associated with alveolar bone remodeling during orthodontic tooth movement: a randomized clinical trial by Alessandro Polizzi, Angela Alibrandi, Antonino Lo Giudice, Alfio Distefano, Laura Orlando, Amer M. Analazi, Giuseppe Pizzo, Giovanni Li Volti, Gaetano Isola

“…Clinical parameters and gingival crevicular fluid (GCF) sampling were performed on both compression and tension sides of a random canine to be distalized (test groups) at baseline and at 1 h, 1 day, 1 month and at 3-month after OTM, while the contralateral canine served as a control group. miRNAs − 7a-3p, -7a-2-3p, -7a-5p, -21-3p, -21-5p, -100-3p, -100-5p, -125b-2-3p, -125b-5p, -200b-3p, and − 200b-5p expression was analyzed using a real-time quantitative polymerase chain reaction (RT-PCR). Data were analyzed to assess miRNAs change following OTM. …”
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SERT-Deficient Mice Fed Western Diet Reveal Altered Metabolic and Pro-Inflammatory Responses of the Liver: A Link to Abnormal Behaviors by Raymond Cespuglio, Anna Gorlova, Konstantin Zabegalov, Kirill Chaprov, Evgeniy Svirin, Kseniia Sitdikova, Alisa Burova, Boris Shulgin, Ksenia Lebedeva, Alexei V. Deikin, Sergey Morozov, Tatyana Strekalova

“…Hepatic pro-inflammatory and metabolism-related gene expression and markers of nitrosative stress, were analyzed utilizing real-time polymerase chain reaction (RT-PCR) and correlated with behavioral and histological outcomes. …”
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Household transmission of SARS-CoV-2 in a rural area in South Africa by G Maimela, C E Martin, M Chersich, B Bello, J Mauti, T Bäernighausen, S Kohler, A Almuedo-Riera, S Luchters, S Sawry

“…Adults with SARS‐CoV‐2 confirmed by polymerase chain reaction (PCR) tests (index cases, ICs) and their household contacts (HCs) were enrolled. …”
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Clinical Significance of Perioperative Minimal Residual Disease Detected by Circulating Tumor DNA in Patients With Lung Cancer With a Long Follow-up Data: An Exploratory Study by Shuta Ohara, MD, PhD, Kenichi Suda, MD, PhD, Sumedha Sudhaman, PhD, Akira Hamada, MD, PhD, Masato Chiba, MD, PhD, Masaki Shimoji, MD, PhD, Toshiki Takemoto, MD, PhD, Ekaterina Kalashnikova, PhD, Samantha K. Cheung, PhD, Michael Krainock, MD, PhD, Jordan Feeney, BS, Himanshu Sethi, MPH, Minetta C. Liu, MD, Junichi Soh, MD, PhD, Yasuhiro Tsutani, MD, PhD, Tetsuya Mitsudomi, MD, PhD

“…A clinically validated, personalized, tumor-informed 16-plex polymerase chain reaction–next-generation sequencing assay was used for the detection and quantification of ctDNA in retrospectively analyzed plasma samples. …”
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SARS-COV-2 re-infection and incidence of post-acute sequelae of COVID-19 (PASC) among essential workers in New York: a retrospective cohort studyResearch in context by Tesleem K. Babalola, Sean A.P. Clouston, Zennur Sekendiz, Devi Chowdhury, Nicola Soriolo, Joseph Kawuki, Jaymie Meliker, Melissa Carr, Bruno R. Valenti, Ashley Fontana, Onix A. Melendez, Olga Morozova, Benjamin J. Luft

“…Methods: We analyzed data from 2511 essential workers, mainly first responders, with confirmed polymerase chain reaction, antibody, or antigen-positive test results for SARS-CoV-2 infection from March 2020 to February 2024. …”
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Infection Frequency and Allelic Variants of <em>Toxoplasma gondii</em> in Wildlife from the Panama Canal Zone by Evelyn Henríquez-Carrizo, Hector Cruz, Alessandra Jurado, Diorene Smith, Delba Villalobos-Cerrud, Lorena Fábrega, Carolina de la Guardia, Ryan Cano, Ricardo Correa, Edy Frías, Anabel Argelis García, Nivia Ríos, Nidia Sandoval, Alex O. Martínez Torres, Armando Castillo-Pimentel, Zuleima Caballero E.

“…The genetic diversity of the <i>T. gondii</i> population contained in the tissues of wild animals was analyzed by the Multilocus Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) technique, using five genes called SAG1, SAG2, SAG3, GRA6, and Apico. …”
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EZH2/miR-142-3p/HMGB1 axis mediates chondrocyte pyroptosis by regulating endoplasmic reticulum stress in knee osteoarthritis by Yang Chen, Shanshan Dong, Xin Zeng, Qing Xu, Mingwei Liang, Guangneng Liao, Lan Li, Bin Shen, Yanrong Lu, Haibo Si, Peifang Wei

“…The expression of the histone methyltransferase enhancer of zeste homolog 2 (EZH2), microRNA-142-3p (miR-142-3p), and high mobility group box 1 (HMGB1) and the levels of ER stress, pyroptosis, and metabolic markers in normal and OA chondrocytes were investigated by western blotting, quantitative polymerase chain reaction, immunohistochemistry, fluorescence in situ hybridization, fluorescein amidite-tyrosine-valine-alanine-aspartic acid-fluoromethyl ketone (FAM-YVAD-FMK)/Hoechst 33342/propidium iodide (PI) staining, lactate dehydrogenase (LDH) release assays, and cell viability assessments. …”
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<i>ACE</i> I/D Genotype and Risk of Non-Contact Injury in Moroccan Elite Athletes: A Pilot Study by El Mokhtar El Ouali, Jihan Kartibou, Juan Del Coso, Rashmi Supriya, Ismail Laher, Zineb El Kettani, Hassan Ghazal, Najib Al Idrissi, Ayoub Saeidi, Abdelhalem Mesfioui, Hassane Zouhal

“…<i>ACE</i> I/D polymorphism genotyping was performed by polymerase chain reaction (PCR) using genomic DNA from blood samples. …”
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4-1BB agonist targeted to fibroblast activation protein α synergizes with radiotherapy to treat murine breast tumor models by Ignacio Melero, Alvaro Teijeira, Christian Klein, Maria E Rodriguez-Ruiz, Christina Claus, Pablo Umana, Tamara Tanos, Carlos E De Andrea, Carlos Luri-Rey, Paloma Sanchez-Mateos, Leticia Fernández-Rubio, María Collantes, Irantzu Serrano-Mendioroz, Celia Barrio-Alonso, Antonio Rullan, Jon Ander Simon, Eneko Garate-Soraluze, Claudia del Pilar Herrero

“…We hypothesized that IR increases FAP expression in CAFs, therefore the combination of IR with targeted immunomodulators such as an agonistic anti-FAP-4-1BBL fusion protein could enhance the immune-mediated antitumoral effects of these treatments.Methods The murine transplantable TS/A tumor-cell-line co-engrafted with CAFs was used to investigate increases in FAP expression in tumors following irradiation using immunohistochemistry, real-time polymerase chain reaction (RT-PCR) and multiplex tissue immunofluorescence. …”
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Identification of Genetic Markers of <i>APOM</i> and <i>CYP7A1</i> Genes Affecting Milk Production Traits in Chinese Holstein by Yanan Liu, Zijiao Guo, Junqing Ni, Chendong Yang, Bo Han, Yabin Ma, Jianming Li, Guie Jiang, Weijie Zheng, Dongxiao Sun

“…By dual-direction sequencing of the polymerase chain reaction (PCR) products of the complete coding sequences and 2000 bp of the 5′ and 3′ flanking regions on pooled DNA sample, seven and three single nucleotide polymorphisms (SNPs) were identified in <i>APOM</i> and <i>CYP7A1</i>, respectively. …”
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Associations of HLA-G 3’UTR polymorphisms and increased HLA-G expression with gastric cancer susceptibility and prognosis by Ines Zemni, Daria Bortolotti, Sabrine Dhouioui, Sana Baroudi, Malek Ferjani, Inès Nasri, Yosr Zenzri, Md Ataur Rahman, Abdel Halim Harrath, Roberta Rizzo, Nadia Boujelbene, Inès Zidi

“…Methods: HLA-G 3’UTR polymorphisms (14pb Insertion/deletion and + 3142C/G) were identified by polymerase chain reaction (PCR) or Sanger sequencing. Plasma levels of sHLA-G (total sHLA-G, shed HLA-G1 and HLA-G5) were determined. …”
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Viral Hepatitis B and Its Implications for Public Health in DR Congo: A Systematic Review by Marcellin Mengouo Nimpa, Hermès Karemere, Christian Ngandu, Franck-Fortune Mboussou, M. Carolina Danovaro-Holliday, Dalau Nkamba, André Bita Fouda, Bienvenu Nguejio, Sifa Kakozi, Aimé Mwana-Wabene Cikomola, Jean-Crispin Mukendi, Dieudonné Mwamba, Moise Désiré Yapi, Richard Bahizire Riziki, Cedric Mwanga, John Otomba, Jean Baptiste Nikiema, Boureima Hama Sambo, Daniel Katuashi Ishoso

“…The Determine rapid test was the most widely used test (10 studies), sometimes combined with ELISA (3 studies) and polymerase chain reaction (PCR) (1 study, for genotyping). …”
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An exploratory study of INPP4B, a biomarker of gemcitabine chemoresistance in pancreatic cancer by REN Jiaqiang, WU Shuai, SU Tong, LI Jie, HAN Liang, WU Zheng

“…Co-expressed genes were screened using real-time fluorescence quantitative polymerase chain reaction (RTFQ-PCR), Western blot and immunohistochemistry to verify the expression levels of target molecules. …”
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Interaction study of the effects of environmental exposure and gene polymorphisms of inflammatory and immune-active factors on chronic obstructive pulmonary disease by Rui Wang, Yuanyuan Li, Yuting Jiang, Xiaona Liu, Hongqi Feng, Zhe Jiao, Bingyun Li, Chang Liu, Yuncheng Shen, Fang Chu, Chenpeng Zhu, Dianjun Sun, Wei Zhang

“…Face-to-face interviews, lung function tests, and multiplex polymerase chain reaction were used to obtain data. Logistic regression model, generalized multifactor dimensionality reduction and crossover analysis were used to analyse the effects of SNPs, gene–gene interactions, and gene-environment interactions on COPD. …”
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Effectiveness of hCMV, mEF1a and mAct promoters on driving of foreign gene expression in transgenic zebrafish by . Alimuddin, G. Yoshizaki, O. Carman, T. Takeuchi

“…Transgenic individuals were identified by polymerase chain reaction (PCR) and their expression levels were analyzed by reverse transcription PCR.  …”
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Development of a one-step multiplex RT-qPCR method for rapid detection of bovine diarrhea viruses by Dequan Yang, Li Ma, Zhongping Yang, Xianchao Yang, Jian Wang, Houbin Ju, Chunguang Lu, Yonggang Weng, Heping Zhao, Haixiao Shen, Xin Li, Feifei Ge, Xiaoxu Wang, Xiujuan Wu, Meng Xiang, Guidan Feng, Congsheng Tang, Shixin Huang, Hongjin Zhao

“…In this study, we developed a one-step multiplex reverse-transcription quantitative real-time polymerase chain reaction (mRT-qPCR) that enables the simultaneous detection of three key viral pathogens responsible for calf diarrhea: bovine kobuvirus (BKoV), bovine astrovirus (BoAstV), and bovine torovirus (BToV). …”
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