COVID-19 Pandemic Experiences in Pediatric Intensive Care Unit: An Iranian Referral Hospital-Based Study by Masoud Mohammadpour, Seyed Abbas Hassani, Meisam Sharifzadeh, Leila Tahernia, Setareh Mamishi, Bahareh Yaghmaie, Zeinab Najafi, Farzaneh Beirami, Mehrnoush Afsharipour, Maryam Minuyeefar, Mina Dolatzadeh, Neda Pak, Anahita Majmaa, Zahra Zamani, Shima Mahmoudi

“…In total, 99 patients were admitted to COVID PICU, 42.4% (42 patients) were males, and 66 patients had positive SARS-CoV-2 real-time reverse transcriptase-polymerase chain reaction (RT-PCR). There was no statistically significant difference in the frequency of clinical signs and symptoms (except for fever) among patients with positive SARS-CoV-2 RT-PCR and negative ones. …”
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Comparison of Maternal, Perinatal, and Neonatal Outcomes of Asymptomatic and Symptomatic Pregnant Women with Coronavirus Disease-2019 by Tayfun VURAL, Burak BAYRAKTAR, Suna YILDIRIM KARACA, Ozan ODABAS, Nisel YILMAZ, Pınar SAMLIOGLU, Mehmet OZEREN

“…COVID-19 was diagnosed using the real-time reverse transcriptase-polymerase chain reaction on the oropharyngeal and nasopharyngeal swabs. …”
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Evaluation of Inflammatory, Cardiac and Hematological Parameters in Hospitalized Children with COVID-19 by Damla Geçkalan, Rahmi Özdemir, Zülal Yılmaz, Onur Sivas, Yasin Yılmaz, Cemile Hilal Çelik

“…All patients’ age, gender, number of hospitalization days, respiratory symptoms, laboratory parameters, thoracic computed tomography, polymerase chain reaction results were recorded. Patients were analyzed for pneumonia presence and absence. …”
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Molecular detection and genomic characterization of Samak Micromys paramyxovirus-1 and -2 in Micromys minutus, Republic of Korea by Augustine Natasha, Sarah E. Pye, Seung Hye Cho, Haryo Seno Pangestu, Jieun Park, Kyungmin Park, Sara P. Prayitno, Bohyeon Kim, Jong Sun Lee, Jongwoo Kim, Shailesh Budhathoki, Yeonsu Oh, Jin-Won Song, Carolina B. López, Jun Gyo Suh, Won-Keun Kim

“…Method Here, we screened M. minutus collected from the Gangwon Province in the ROK for paramyxoviruses using nested polymerase chain reaction and confirm positive samples by next-generation metagenomic sequencing. …”
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Deciphering salt-responsive NB-ARC genes in rice transcriptomic data: A bioinformatics approach with gene expression validation by Saputro Triono Bagus, Wardhani Fila Oxi, Sholihah Nur Fadlilatus, Ermavitalini Dini, Purnama Putut Rakhmad, Nurhidayati Tutik

“…Two varieties with contrasting tolerance levels were selected for validation. Real-time polymerase chain reaction was employed to determine the gene expression for validation. …”
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Evidence of vancomycin-resistant Staphylococcus aureus, multidrug-resistant S. aureus, and Enterococcus faecium-causing mastitis in Thailand and Cambodia by Sambo Na, Montira Intanon, Anyaphat Srithanasuwan, Wasana Chaisri, Witaya Suriyasathaporn

“…Disk diffusion tests were conducted for antibiotic susceptibility, and polymerase chain reaction was employed to detect AMR genes. …”
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Prognostic Impact of miR-224 and RAS Mutations in Medullary Thyroid Carcinoma by Elisabetta Cavedon, Susi Barollo, Loris Bertazza, Gianmaria Pennelli, Francesca Galuppini, Sara Watutantrige-Fernando, Simona Censi, Maurizio Iacobone, Clara Benna, Federica Vianello, Stefania Zovato, Davide Nacamulli, Caterina Mian

“…Quantitative real-time polymerase chain reaction was used to quantify mature hsa-miR-224 in tumor tissue. …”
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Comparative evaluation of various DNA extraction methods and analysis of DNA degradation levels in commercially marketed Chestnut rose juices and beverages by Yongchao Ren, Yunlong Ma, Yanqi Li, Yun Song, WeiWei Zhao, Xuncai Huang, Danmin Yu, Jian Li, Zuogang Xu, Wenjun Zhao

“…Methods The quantity and quality of the extracted DNA were assessed using NanoDrop One spectrophotometer, gel electrophoresis, and real-time polymerase chain reaction (real-time PCR or qPCR) assays. An assessment was conducted on the processing time, labor intensity, and cost associated with each approach. …”
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Visfatin Induces Inflammation and Insulin Resistance via the NF-κB and STAT3 Signaling Pathways in Hepatocytes by Yu Jung Heo, Sung-E Choi, Ja Young Jeon, Seung Jin Han, Dae Jung Kim, Yup Kang, Kwan Woo Lee, Hae Jin Kim

“…After treatment with visfatin, the inflammatory cytokines IL-6, TNF-α, and IL-1β were assessed by real-time polymerase chain reaction (RT-PCR) and immunocytochemical staining in HepG2 cells. …”
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Occurrence of macrolides resistance in Legionella pneumophila ST188: Results of the Belgian epidemiology and resistome investigation of clinical isolates by Charlotte Michel, Fedoua Echahidi, Geraldine De Muylder, Max Sewell, Ian Boostrom, Olivier Denis, Owen B. Spiller, Denis Pierard

“…Urinary antigen testing remains the main used test, but polymerase chain reaction and serology allow the diagnostic in 14.8% and 2.4% of cases, respectively. cgMLST showed a good discrimination between sequence typing (ST) and minimal variation for ST47 isolates, whereas ST1s were more diverse. …”
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Inhibition of complement system-related gene ITGB2 attenuates epithelial–mesenchymal transition and inflammation in diabetic nephropathy by Jun Peng, Wenqi Zhao, Lu Zhou, Kun Ding

“…Quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) and western blotting were utilized to detect the ITGB2 expression. …”
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Low Serum miR-607 Level as a Potential Diagnostic and Prognostic Biomarker in Patients of Pancreatic Ductal Adenocarcinoma: A Preliminary Study by Dawei Jiang, Xiangfei Yuan, Jianqi Ni, Lan Shen, Min Cai, Liu Xu

“…The expression levels of miR-607 were assessed by quantitative real-time polymerase chain reaction (qRT-PCR). The correlation between miR-607 expression and clinical characteristics was analyzed by the Chi-square test. …”
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SARS-CoV-2 Ct values and COVID-19 symptoms in patients with haematological malignancies in South Africa by Keitumetsi L. Sothoane, Sarah A. Van Blydenstein, Vinitha Philip, Jeannette Wadula

“…Background: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) reverse transcriptase polymerase chain reaction (RT-PCR) cycle threshold (Ct) values serve as surrogate markers for estimating viral load. …”
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Clinical Evaluation of a Rapid Reciprocal-Flow PCR Assay and Real-Time PCR Assay with Quenching Probe for Detection of <i>Mycobacterium tuberculosis</i> Complex by Kosuke Kosai, Keisuke Matsumoto, Takahisa Ishikawa, Yasuhide Kawamoto, Norihiko Akamatsu, Kenji Ota, Fujiko Mitsumoto-Kaseida, Norihito Kaku, Hiroo Hasegawa, Koichi Izumikawa, Hiroshi Mukae, Katsunori Yanagihara

“…This study investigated the diagnostic efficiencies of two assays for the detection of <i>Mycobacterium tuberculosis</i> complex: (1) the reciprocal-flow real-time polymerase chain reaction (PCR)-based GeneSoC assay and (2) the real-time PCR based GENECUBE MTB assay with quenching probe. …”
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Identification of immune-associated genes with altered expression in the spleen of mice enriched with probiotic species using RNA-seq profiling by Anh Duc Truong, Ha Thi Thanh Tran, Nhu Thi Chu, Lanh Phan, Hoai Thi Phan, Thu Huong Dang, Hoang Vu Dang, La Anh Nguyen

“…On the other hand, 12 toll-like receptor genes underwent additional validation through quantitative real-time polymerase chain reaction (qRT-PCR), affirming the increased alignment between qRT-PCR and RNA-Seq findings. …”
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Determining the mechanism of Shuxuening injection against liver cirrhosis through network pharmacology and animal experiments by Qiyao Liu, Tingyu Zhang, Yongan Ye, Xin Sun, Huan Xia, Xu Cao, Xiaoke Li, Wenying Qi, Yue Chen, Xiaobin Zao

“…Serum and liver tissues of the mice were collected for the detection of indicators, pathological staining, and expression analysis of hub targets using quantitative real-time polymerase chain reaction (qRT-PCR). Results: We identified 368 overlapping genes (OLGs) between SXN and LC targets. …”
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Ecdysone receptor strongly influences larval–pupal–adult transition and melanization in Tuta absoluta by Xiaodi Wang, Jiajia Wu, Jianyang Guo, Nianwan Yang, Fanghao Wan, Zhichuang Lü, Wanxue Liu

“…Methods In this study, RNA extraction, cDNA synthesis, gene cloning, bioinformatics analysis, and quantitative real-time polymerase chain reaction were used to obtain the full length, conserved domain, and relative expression levels of the EcR. …”
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Isolation and molecular detection of Peste des petits ruminants virus in an outbreak investigation in Benishangul Gumuz, Ethiopia by Mengesha Abebe, Yimer Lama, Aliy Abdi, Shegu Dereje, Abdeta Debela, Waktole Hika, Hirpa Tola Eyob

“…Altogether, 180 clinically diagnosed and 32 shoats with typical clinical sign of PPR were identified for laboratory confirmation Nasal and rectal swabs from 32 sheep and goats were collected and cultured on Verodog SLAM (VDS) cell line and tested using then tested using a quantitative real-time polymerase chain reaction (RT_PCR) assay and conventional PCR with 1.5% gel electrophoresis for detection of the N gene. …”
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Clinical and neurological specifics of the vertebrogenic lumbosacral radiculopathy course in the patients with RS1143627 polymorphism of the <i>IL-1β</i> gene by Elena A. Statinova, Natalya V. Fominova, Maria S. Kishenya

“…The rs1143627 polymorphism was identified by real-time polymerase chain reaction (IQ5 amplifier (Bio-Rad, USA)) with the SNP-express (IL-1β-31C/T) test system (Litech, Russia). …”
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Identification and Analysis of Potential Immune-Related Biomarkers in Endometriosis by Yanan He, Jixin Li, Yanjun Qu, Liyuan Sun, Xibo Zhao, Han Wu, Guangmei Zhang

“…Then, we used quantitative real-time polymerase chain reaction (qRT-PCR) to verify the hub genes. …”
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