Protective Effect of Black Bean-Whey Double Protein Diet on Intestinal Barrier Injury Induced by Lipopolysaccharide in Rats by Weihong MENG, Zhiming LI, Jiayu ZHANG, Xin SHU, Defu LIANG, Kejin ZHUANG, Dongjie ZHANG

“…The expression of tight junction proteins was quantified using immunohistochemistry, and the TLR4 and MyD88 expression levels were determined using real-time fluorescence quantitative polymerase chain reaction. Results showed that compared with low- and high-dose B-W DP, intermediate-dose B-W DP significantly (P<0.01) protected against intestinal barrier injury caused by LPS, increased villus length by 26.69%, reduced crypt depth by 22.61%, and increased their ratio by 46.78%. …”
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Comparison of Community-Associated and Health Care-Associated Methicillin-Resistant Staphylococcus aureus in Canada: Results from CANWARD 2007 by Kimberly A Nichol, Melissa McCracken, Melanie R DeCorby, Kristjan Thompson, Michael R Mulvey, James A Karlowsky, Daryl J Hoban, George G Zhanel

“…Strain typing was performed by pulsed-field gel electrophoresis (PFGE) and the mecA, nuc and pvl genes were detected by polymerase chain reaction. RESULTS: Of the 385 MRSA, 19.5% were CA-MRSA and 79.2% were HA-MRSA as determined by PFGE. …”
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Mechanism of Action of Decitabine in the Treatment of Acute Myeloid Leukemia by Regulating LINC00599 by Fan Du, Ting Jin, Li Wang

“…Reverse transcription polymerase chain reaction (RT-PCR) was performed to examine the expression of lncRNA LINC00599. …”
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Human Umbilical Cord Mesenchymal Stem Cells Encapsulated with Pluronic F-127 Enhance the Regeneration and Angiogenesis of Thin Endometrium in Rat via Local IL-1β Stimulation by Shuling Zhou, Yu Lei, Ping Wang, Jianying Chen, Liting Zeng, Ting Qu, Martin Maldonado, Jihua Huang, Tingting Han, Zina Wen, Erpo Tian, Xiangqian Meng, Ying Zhong, Jiang Gu

“…Angiogenesis capacity of interleukin-1 beta (IL-1β)-primed hUC-MSCs was monitored via quantitative polymerase chain reaction (q-PCR), Luminex assay, and tube formation assay. …”
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Diversity in Adaptive Evolution of Methicillin-Resistant Staphylococcus aureus Clinical Isolates Under Exposure to Continuous Linezolid Stress in vitro by Han T, Jia T, Wang J

“…MRSA isolates with different minimum inhibitory concentration (MIC) values for linezolid were randomly selected to perform the assay of adaptive laboratory evolution with sub-inhibitory concentrations of linezolid. Polymerase chain reaction assays and sequencing techniques were performed to detect well-known molecular determinants related to linezolid resistance, including the expression of optrA and cfr, mutations of 23S rRNA gene and ribosomal protein (L3, L4, L22) encoding genes (rplC, rplD, rplV).Results: After induction with sequentially increasing concentrations of linezolid, all four MRSA strains (L914, L860, L1096, and L2875) evolved into linezolid-resistant strains over various induction times (480, 384, 288, and 240 h) and universally formed small colony variants. …”
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Adjunctive diagnostic tool for histopathological classification of congenital mesoblastic nephroma based in molecular genetic findings by Hiroshi Hamada, Kenichi Kohashi, Takeshi Iwasaki, Mikiko Hashisako, Yuko Hino, Masahiro Fukuhara, Amane Kamouchi, Naonori Kawakubo, Tatsuro Tajiri, Yoshinao Oda

“…Reverse transcription-polymerase chain reaction was performed for the NTRK gene fusion and EGFR-ITDs in all cases. …”
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Role of the Notch signaling pathway in porcine oocyte maturation by Pil-Soo Jeong, Hyo-Gu Kang, Dabin Cha, Se-Been Jeon, Min Ju Kim, Bong-Seok Song, Bo-Woong Sim, Sanghoon Lee

“…Methods Reverse transcription-polymerase chain reaction (RT-PCR) and immunocytochemical assays were used to determine the existence of Notch signaling pathway-related transcripts and proteins in porcine cumulus–oocyte complexes (COCs). …”
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Lipid Coating Modulates Effects of Nanoceria on Oxidative Metabolism in Human Embryonic Lung Fibroblasts: A Case of Cardiolipin by Elena V. Proskurnina, Madina M. Sozarukova, Elizaveta S. Ershova, Ekaterina A. Savinova, Larisa V. Kameneva, Natalia N. Veiko, Maria A. Teplonogova, Vladimir P. Saprykin, Vladimir K. Ivanov, Svetlana V. Kostyuk

“…We used an MTT assay, fluorescence microscopy, real-time reverse transcription polymerase chain reaction, and flow cytometry. At a concentration of 1.5 μM, bare and cardiolipin-coated nanoceria penetrated into cells within 1–3 h. …”
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Sleep-Aiding Effect of Casein-Derived Peptide TPTLN and Its Underlying Mechanism by DENG Shijie, LIU Ling, HUANG Ju, FENG Fengqin, ZHAO Minjie, DU Juan

“…Enzyme-linked immunosorbent assay (ELISA) was used to determine the levels of 5-hydroxytryptamine (5-HT), γ-aminobutyric acid (GABA), 5-hydroxyindoleacetic acid (5-HIAA), glutamate (Glu), β-endorphin (β-EP), cortisol, norepinephrine (NE) in the serum and brain tissue, and the GABA/Glu ratio was evaluated. real-time quantitative polymerase chain reaction (qPCR) was used to detect the effect of TPTLN on the mRNA relative expression levels of γ-aminobutyric acid A receptor α1 (GABAA-α1) and 5-hydroxytryptamine receptor 1A (5-HT1A) in the hypothalamus. …”
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Maternal and neonatal outcomes of Group B Streptococcus colonization: a retrospective study by Guixiu Jin, Lanhua Liu, Xiaolong Wang, Junling Fei, Zhenling Zhu, Ziyan Jiang, Min Liu

“…Pregnant women between 35 and 37 weeks of gestation had GBS screening using real-time polymerase chain reaction (RT-PCR). The clinical characteristics and outcomes of mothers and newborns were collected. …”
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Visible and rapid detection of feline chaphamaparvovirus using multienzyme isothermal rapid amplification and lateral flow dipstick assay by Jun Ji, Xinhao Mu, Shunshun Pan, Xin Xu, Shiyuan Zhang, Honghui Huang, Ying Li, Yingzuo Bi, Lunguang Yao

“…The newly established MIRA-LFD assay had a detection limit of 32.3 copies/μL, which was 10-fold lower than that of the nested polymerase chain reaction (PCR) assay. Furthermore, the MIRA-LFD assay detected 29 FeChPV-positive samples among 417 cats with diarrhea, providing a slightly higher positivity rate than the nested PCR assay. …”
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Targeted detection of sequence variants in cell-free DNA from cerebrospinal fluid in pediatric central nervous system tumors by Katrina O’Halloran, Katrina O’Halloran, Erin E. Crotty, Eirini Christodoulou, Sarah E. Leary, Alexandra Miller, Alexandra Miller, Vera A. Paulson, Christina M. Lockwood, Ashley S. Margol, Ashley S. Margol, Jaclyn A. Biegel, Jaclyn A. Biegel

“…These include polymerase chain reaction (PCR), both droplet digital PCR and reverse transcription-based PCR, Sanger sequencing, and next-generation sequencing approaches that incorporate amplicon- and hybrid capture-based methods. …”
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Decreased Spp1 Expression in Acute Myocardial Infarction after Ischemia and Reperfusion Injury by Ling Li, Jungang Huang, Zongkai Zhao, Zhuzhi Wen, Kang Li, Tianjiao Ma, Lisui Zhang, Junmeng Zheng, Shi Liang

“…In the end, we used quantitative reverse transcription-polymerase chain reaction (qRT-PCR) and western blotting to measure and compare the expression of Spp1 in patients who accepted percutaneous coronary intervention. …”
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Antimicrobial resistance in clinical <I>Escherichia coli</I> isolates obtained from animals by M. N. Isakova, O. V. Sokolova, N. A. Bezborodova, A. S. Krivonogova, A. G. Isaeva, V. D. Zubareva

“…Disk diffusion method was used to study antimicrobial resistance of the cultures; minimum inhibitory concentrations of antimicrobials were determined in a serial dilution method; resistance genes were detected by polymerase chain reaction. The carried out research demonstrates a wide distribution of the isolates belonging to the phenotype resistant to ansamycins (rifampicin), semi-synthetic penicillins (ampicillin and amoxicillin), tetracyclines (doxycycline). …”
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Genetic diversity and antifungal susceptibilities of environmental Cryptococcus neoformans and Cryptococcus gattii species complexes by Mohamed Taha, Yasmine H. Tartor, Rana M Abd Elaziz, Ibrahim Elsohaby

“…These isolates were genotyped using M13 polymerase chain reaction-fingerprinting and URA5 gene restriction fragment length polymorphism analysis. …”
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Unraveling the genetic mysteries of spinal muscular atrophy in Chinese families by Shanshan Gao, Duo Chen, Qianqian Li, Xuechao Zhao, Chen Chen, Lina Liu, Miao Jiang, Zhenhua Zhao, Yanhua Wang, Xiangdong Kong

“…Various molecular techniques, including multiplex ligation-dependent probe amplification analysis, long-range polymerase chain reaction (PCR) combined with nested PCR, Sanger sequencing, and whole-exome sequencing were employed to establish a thorough genetic variant profile in 680 Chinese pedigrees with clinically suspected SMA. …”
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Evaluating strategies for global respiratory pandemic control at ports: a modelling study by Gregory Gan, Jue Tao Lim, Tong Guan, Sharon Esi Duoduwa, S Balakumar, Janhavi A, Borame L Dickens

“…To minimise this for future pandemic preparedness, we explore the use of quarantining and testing of crews preboarding instead of quarantining at port calls or during voyages.Methods Using a microsimulation model of 10 000 vessels and 300 000 crew, we examine the effectiveness of nine predeparture control strategies in reducing COVID-19 outbreak sizes, from no control to the use of 7-day and 14-day quarantining, and addition of polymerase chain reaction (PCR) or antigen rapid test (ART) testing. …”
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Lack of Association of the Polymorphisms IL-17A (−197G/A) and IL-17F (+7488A/G) with Multibacillary Leprosy in Mexican Patients by Mónica Escamilla-Tilch, Iris Estrada-García, Julio Granados, Roberto Arenas-Guzmán, Rosalio Ramos-Payan, Thalía Gabriela Pérez-Suárez, Ma. Isabel Salazar, Riky Luis Pérez-Lucas, Sergio Estrada-Parra, Nora Magdalena Torres-Carrillo

“…Both SNPs were genotyped with the polymerase chain reaction-restriction fragment length polymorphism technique. …”
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Association of three single nucleotide polymorphisms in the <i>LPIN1</i> gene with milk production traits in cows of the Yaroslavl breed by A. V. Igoshin, T. M. Mishakova, R. B. Aitnazarov, A. V. Ilina, D. M. Larkin, N. S. Yudin

“…Genotyping of SNPs was carried out by polymerase chain reaction-restriction fragment length polymorphism method. …”
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ELAVL1‐dependent SOAT2 exacerbated the pancreatitis‐like cellular injury of AR42J cells induced by hyperstimulation with caerulein by Yu‐Jing Sun, Hua‐Ying Chen, Xiao‐Qin Lai

“…Protein and mRNA expression were evaluated through western blotting and quantitative real‐time polymerase chain reaction, respectively. Furthermore, an RNA immunoprecipitation assay was conducted to investigate the association between ELAV‐like RNA binding protein 1 (ELAVL1) and SOAT2. …”
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