Dexamethasone Provides Effective Immunosuppression for Improved Survival of Retinal Organoids after Epiretinal Transplantation by Bikun Xian, Ziming Luo, Kaijing Li, Kang Li, Mingjun Tang, Runcai Yang, Shoutao Lu, Haijun Zhang, Jian Ge

“…Following this, we used immunofluorescence, real-time polymerase chain reaction, and flow cytometry to investigate the effects of DEX and RAP on cells in the retinal organoids. …”
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The potential role of differentially expressed tRNA-derived fragments in high glucose-induced podocytes by Zhenxing Zhang, Yunyang Qiao, Jialing Ji, Chan Huang, Huimin Shi, Weihua Gan, Aiqing Zhang

“…The 10 tRFs with the highest differential expression were detected by real-time quantitative polymerase chain reaction (RT-qPCR), and these results were consistent with the sequencing results. …”
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Prevalence of Avian Pathogenic Escherichia coli (APEC) Clone Harboring sfa Gene in Brazil by Terezinha Knöbl, Andrea Micke Moreno, Renata Paixão, Tânia Aparecida Tardelli Gomes, Mônica Aparecida Midolli Vieira, Domingos da Silva Leite, Jesus E. Blanco, Antônio José Piantino Ferreira

“…Escherichia coli sfa+ strains isolated from poultry were serotyped and characterized by polymerase chain reaction (PCR) and amplified fragment length polymorphism (AFLP). …”
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Molecular Characterization and Clinical Characteristics of m5C-Based RNA Methylation in Spinal Cord Injury: Validated by qPCR by Liang Cao, Wen Jun Pi, Qiang Zhang, Qing Li

“…We demonstrated their importance between SCI group and sham group using real-time reverse transcription-quantitative polymerase chain reaction in rat model. Expression of hub genes did not correlate with mitophagy but was positively correlated with endoplasmic reticulum stress (ERS), which suggested that there may be differences in ERS between different patterns of m5C-based modification. …”
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Rational strategies for designing next-generation oncolytic viruses based on transcriptome analysis of tumor cells infected with oncolytic herpes simplex virus-1 by Naeme Javid, Shahriyar Abdoli, Majid Shahbazi, Majid Shahbazi

“…Subsequently, the expression of hub genes was verified by real-time polymerase chain reaction (qRT-PCR) in MDA-MB-231 (a breast cancer cell line) infected with oHSV-1 and non-infected cells.ResultsThe results of our data analysis indicated notable disparities in the genes associated with the proteasome pathway between infected and non-infected cells. …”
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PLCG2 and IFNAR1: The Potential Biomarkers Mediated by Immune Infiltration and Osteoclast Differentiation of Ankylosing Spondylitis in the Peripheral Blood by Bo Han, Qiaobo Xie, Weishi Liang, Peng Yin, Xianjun Qu, Yong Hai

“…RNA extraction reverse transcription and quantitative polymerase chain reaction were conducted in the peripheral blood collected from six AS patients and six health volunteers matched by age and sex. …”
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The Association Between Adiponectin Gene Polymorphism (rs1501299) and Metabolic Syndrome by Yin L, Tang Y, Yuan Y

“…The polymorphisms of the G/T gene at the rs1501299 locus of the APN gene were detected using restriction fragment length polymorphism polymerase chain reaction technology.Results: The serum APN levels were significantly lower in Mets patients than in the control group (15.0 ± 4.9 mg/L vs 27.2 ± 6.5 mg/L, p < 0.05). …”
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Role of B7H4 and Fas in regulation of trophoblast cells and development of pre-eclampsia: a cross-sectional study by Xiujuan Dong, Cuicui Nie, Yanli Yin, Haixia Xu, Yanxia Zhang, Lihua Han, Congcong Xu

“…The expression levels of B7H4 and Fas were analysed using western blotting and real-time quantitative polymerase chain reaction (RT-qPCR). The upstream regulators of B7H4 in trophoblasts were predicted and estimated using a luciferase reporter assay. …”
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Amelioration of Oxidative Stress in Rats with Chronic Obstructive Pulmonary Disease through Shenqi Huatan Decoction Activation of Peroxisome Proliferator-Activated Receptor Gamma-M... by Jingjing Chen, Wenxiao Qiao, Xiaoming Xue, Dian Li, Ye Zhang, Di Xie, Jinyun Wang, Yaoqin Sun, Shuo Yang, Zhuomin Yang

“…Activated protein kinase alpha (AMPK-α), forkhead transcription factor O3a (FOXO3a), manganese SOD (MnSOD), and peroxisome proliferator-activated receptor gamma (PPARγ) were detected using reverse transcriptase quantitative polymerase chain reaction (RT-qPCR) and Western blot. Microribonucleic acid and protein expression levels were measured, and pathological changes in lung tissue were observed using hematoxylin and eosin staining. …”
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Erbin Regulates Tissue Factors Through Ras/Raf Pathway in Coagulation Disorders in Sepsis by Yang C, Lei C, Jing G, Xia Y, Zhou H, Wu D, Zuo J, Gong H, Wang X, Dong Y, Aidebaike D, Wu X, Song X

“…However, the role of Erbin in coagulation disorders in sepsis and the relationship between Erbin and macrophage regulation of coagulation function are still unclear.Methods: At the cellular level, macrophages were treated with lipopolysaccharide (LPS) or MEK inhibitor (PD98059), protein expression levels were detected by Western blot, co-immunoprecipitation (Co-IP), and immunofluorescence, mRNA expression levels were detected by quantitative real-time polymerase chain reaction (qPCR), and the concentration of tissue factor (TF) in cell supernatant was detected by enzyme linked immunosorbent assay (ELISA). …”
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Molecular Detection of Antibiotic-Resistant Genes in Pseudomonas aeruginosa from Nonclinical Environment: Public Health Implications in Mthatha, Eastern Cape Province, South Africa by Mojisola Clara Hosu, Sandeep Vasaikar, Grace Emily Okuthe, Teke Apalata

“…Molecular characterization of the isolates was confirmed using real-time polymerase chain reaction (rPCR) and selected isolates were further screened for the possibility of harboring antimicrobial resistance genes. …”
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Topical neurokinin-1 receptor antagonism ameliorates ocular pain and prevents corneal nerve degeneration in an animal model of dry eye disease by Amirreza Naderi, Yukako Taketani, Shudan Wang, Francesca Kahale, Ann Yung, Pier Luigi Surico, Yihe Chen, Reza Dana

“…Trigeminal ganglions (TG) were collected to determine SP levels by ELISA and transient receptor potential cation channel subfamily V member 1 (TRPV1), transient receptor potential cation channel subfamily M (melastatin) member 8, c-Fos, and activating transcription factor 3 (ATF3) mRNA levels by real-time polymerase chain reaction. Results:. Treating DED mice with L-733,060 resulted in a significant reduction in eye wipe behavior, a significant increase in palpebral ratio, and significant decreases in SP levels in both the cornea and TG compared with the vehicle-treated group. …”
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Association between Virulence Factors and TRAF1/4-1BB/Bcl-xL Expression in Gastric Mucosa Infected with Helicobacter pylori by Fen Wang, Xiang Wu, Zhiying Liu, Guangkui Bu, Xiayu Li, Nanfang Qu, Jin Peng, Canxia Xu, Shourong Shen, Yi Yuan

“…The expression of cagA, vacAs1, and vacAm1 in H. pylori was examined with polymerase chain reaction. Results. The expression of TRAF1, 4-1BB, and Bcl-xL was significantly upregulated in IM and Ca patients (P<0.05 compared with CG). …”
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Mechanism of action of Xipayimaizibizi oral liquid on outlet obstruction-induced overactive bladder: An integrated study by Menglu Wang, Yang Yang, Yuhang Du, Jiamei Xie, Yige Zhao, Yongcheng An, Ziyi Shan, Shenyujun Wang, Meng Hao, Baosheng Zhao

“…The key targets involved in these mechanisms were validated using reverse transcription-quantitative polymerase chain reaction, immunohistochemistry, and western blot in vivo/vitro experiments. …”
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Chemokine Receptor 2 (CXCR2) Gene Variants and Their Association with Periodontal Bacteria in Patients with Chronic Periodontitis by Denisa Kavrikova, Petra Borilova Linhartova, Svetlana Lucanova, Hana Poskerova, Antonin Fassmann, Lydie Izakovicova Holla

“…A total of 500 unrelated subjects participated in this case-control study. 329 CP patients and 171 healthy nonperiodontitis controls were analyzed using polymerase chain reaction techniques for three single-nucleotide polymorphisms (SNPs): +785C/T (rs2230054), +1208T/C (rs1126579), and +1440A/G (rs1126580). …”
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Gene expression in gastric biopsies from patients with Helicobacter pylori infection by D. Davoudi Oskouei, H. Akpınar, S. Sarıoğlu, P. Keskinoğlu, M. Soytürk, İ. Şimşek, Ö. Yılmaz

“…Biopsy specimens from 101 patients were analyzed for H. pylori cagA, omp6, omp13, omp18, omp20, 16S rRNA, and ureA gene expression by Real-Time Reverse Transcription-Polymerase Chain Reaction (RT-PCR). Total RNA was extracted from antral and corpus biopsies and cDNA was synthesized. …”
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Clinical spectrum of disease and outcomes in children with Omicron SARS-COV-2 infection in Cape Town, South Africa by C. Bekker, I. Dewandel, A. Redfern, C. McKenzie, J. Lishman, L. M. Verhagen, M. Claassen, S. Wilson, R. Dunbar, C. Bosch, G. van Zyl, W. Preiser, P. Goussard, H. Rabie, M. M. van der Zalm

“…METHODS: We analysed routine care data from a prospective cohort of children aged 0–13 years, with a positive SARS-CoV-2 real-time reverse-transcription polymerase chain reaction (rRT-PCR) or SARS-CoV-2 antigen test, admitted to Tygerberg Hospital between 1 November 2021 until 1 March 2022. …”
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Effects of early neonatal proinflammatory stress on the expression of BDNF transcripts in the brain regions of prepubertal male rats by D. I. Peregud, S. V. Freiman, A. O. Tishkina, L. S. Sokhranyaeva, N. A. Lazareva, M. V. Onufriev, M. Y. Stepanichev, N. V. Gulyaeva

“…BDNF polypeptide concentration was estimated by means of an enzyme-linked immunosorbent assay, while quantitative polymerase chain reaction followed by reverse transcription was used to detect exon-specific BDNF mRNA expression. …”
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Comparison of glimepiride and linagliptin in the treatment of non-alcoholic hepatic disease with type 2 diabetes mellitus by Feng Tian, Yali Guo, Liping Zhou, Qunying Yao, Xiaoyan Liang, Jiaxin Lu, Aiping He, Jie Shen

“…Furthermore, real-time quantitative polymerase chain reaction was used to evaluate the expression level of miR-210 in the patients’ serum. …”
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DIAGNOSTIC VALUE OF LABORATORY TESTS OF WOMEN IN THE DYNAMICS OF TREATMENT OF PERIODONTAL DISEASES CONCOMITANT WITH BACTERIAL VAGINOSIS by T.O. Petrushanko, A.D. Krutikova, E.I. Krutikova

“…The presence of Gardnerella vaginalis and Atopobium vaginae in the oral cavity was determined by the polymerase chain reaction (PCR) method. Results. The sIgA level of women with BV was by 30-45% lower as compared to the value of patients without BV. …”
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