Uncovering genetic determinants of antioxidant properties in Thai landrace rice through genome-wide association analysis by Putut Rakhmad Purnama, Pipob Suwanchaikasem, Supaporn Junbuathong, Somsong Chotechuen, Peerapon Moung-Ngam, Waraluk Kasettranan, Chanita Paliyavuth, Monnat Pongpanich, Sittiruk Roytrakul, Luca Comai, Teerapong Buaboocha, Supachitra Chadchawan

“…Two genetic markers for polymerase chain reaction detection were developed for the OsTT8 gene and the OsRc promoter, both of which were positively correlated with antioxidant properties. …”
Get full text

Effects of Adipose-tissue Derived Mesenchymal Stem Cells on PBMCs in Co-culture with HeLa Cell Line by Maryam Dorfaki, mahdi ghatrehsamani, fahimeh Lavi Arab, Mona Roozbehani, Majid Khoshmirsafa, Reza Falak, Fatemeh Keshavarz, Fatemeh Faraji

“…Materials and Methods: The effect of MSC cells on proliferation, apoptosis, and gene expression of the cytokines tumor necrosis factor alpha (TNF-α), transforming growth factor beta (TGF-β), interleukin (IL)-4, IL-2, and interferon-γ in PBMCs cultured together with HeLa cells was investigated using flow cytometry and real-time polymerase chain reaction (PCR), respectively. Results: Flow cytometry showed that co-culture of PBMCs/MSCs/HeLa significantly increased the proliferation of PBMCs at different time points, with a p-value of 0.0022. …”
Get full text

Implications of cytokine genes polymorphisms in Jordanian patients with obsessive compulsive disorder by Ahmad M. Khalil, Manal W. Khrais, Khaled M. Al-Qaoud, Mohamad Y. Al Ta’any

“…Five polymorphic positions in four interleukin genes (IL-1β; rs16944 and rs1143634, IL-6; rs1800795, IL-10; rs1800896 and rs1800795) were genotyped using polymerase chain reaction–restriction fragment length polymorphism (PCR–RFLP) method. …”
Get full text

Newcastle disease virus genotype VII.1.1 identified from backyard chickens with low antibody titer: Jimma Zone, Southwest Ethiopia. by Bezina Arega Emeru, Haregawi Tesfaye Desta, Yosef Deneke

“…From the swab samples, total RNA was extracted and the viral genomic material was detected by amplifying the Fusion gene by Reverse transcription polymerase chain reaction (RT-PCR). The interconnection between ELISA and RT-PCR was also analyzed. …”
Get full text

The Differential Effects of Leukocyte-Containing and Pure Platelet-Rich Plasma on Nucleus Pulposus-Derived Mesenchymal Stem Cells: Implications for the Clinical Treatment of Interv... by Jun Jia, Shan-zheng Wang, Liang-yu Ma, Jia-bin Yu, Yu-dong Guo, Chen Wang

“…NPMSCs isolated from rabbit NP tissues were treated with L-PRP or P-PRP in vitro, and then cell proliferation and expression of stem cell markers, proinflammatory cytokines (TNF-α, IL-1β), production of ECM (extracellular matrix-related protein), and NF-κB p65 protein were validated by CCK-8 assay, real-time polymerase chain reaction, enzyme-linked immunosorbent assay, immunofluorescence, and western blot respectively. …”
Get full text

The MADS-Box Transcription Factor ClAG2 Is a Key Regulator for the Formation of Double Flower in <i>Clematis</i> L. by Xuerong Chen, Ping Zhou, Nanhong Guo, Yiping Zheng, Xiumei Hou, Lihui Zeng

“…Quantitative real-time polymerase chain reaction (qRT-PCR) analysis revealed tissue-specific expression patterns among these genes. …”
Get full text

Utility of Integrated Analysis of Pharmacogenomics and Pharmacometabolomics in Early Phase Clinical Trial: A Case Study of a New Molecular Entity by Jaeseong Oh, Sojeong Yi, Namyi Gu, Dongseong Shin, Kyung-Sang Yu, Seo Hyun Yoon, Joo-Youn Cho, In-Jin Jang

“…The pharmacogenomics screening was performed from whole blood samples using the Affymetrix DMET (Drug-Metabolizing Enzymes and Transporters) Plus microarray, and confirmation of genetic variants was performed using real-time polymerase chain reaction. Metabolomics profiling was performed from plasma samples using liquid chromatography coupled with quadrupole time-of-flight mass spectrometry. …”
Get full text

The Association between Deoxyribonucleic Acid Hypermethylation in Intron VII and Human Leukocyte Antigen-C∗07 Expression in Patients with Endometriosis by Wenrong Zhao, Lei Lei, Rui Chen, Yanmin Zhang, Linlin Chang, Jingxin Cheng

“…The differential DNA methylation of the selected genes was validated via direct bisulfite sequencing and analysis of their messenger ribonucleic acid (mRNA) levels using quantitative reverse transcription polymerase chain reaction (qRT-PCR). Results. Fifteen differentially methylated CpG sites were found among the patients and healthy women, and five CpG sites were mapped to the introns of the human leukocyte antigen-C (HLA-C) gene; these were highly polymorphic between different HLA-C alleles and were HLA-C∗07 specific. …”
Get full text

miR-576-5p Promotes the Proliferation of Papillary Thyroid Carcinoma through the MAPK4-AKT Pathway by Rui Hai, Yang Zhou, Fan Li, Qingxi Guo, Yang Long, Fang Wang, Lian Cheng, Fei Wu, Shi Chen, Xiangyu Zhou

“…Expression levels of miR-576-5p in PTC patient tissues and cell lines were determined by reverse transcription-quantitative polymerase chain reaction (qRT‒PCR). Cell counting using cell counting kit-8 (CCK-8), wound healing, and Transwell assays were performed to evaluate the effect of miR-576-5p on the proliferation, migration, and invasion of TPC-1 cells. …”
Get full text

Are there benefits of culture-based detection of Mycobacterium avium spp paratuberculosis over histopathology? by Motlatso T. Hlokwe, Nomawethu S. Masina, Boitumelo Letsoko, Sewellyn C. Davey, Anita L. Michel

“…Deoxyribonucleic acid (DNA) extracted from culture isolates was subjected to polymerase chain reaction (PCR) amplification using primers that target the IS900 regions of the MAP for species verification. …”
Get full text

The clinical role of blood coagulation and platelet receptors gene allelic variants in development of Wilson’s disease by T. P. Rozina, S. V. Fastovets, E. E. Starostina, L. M. Samokhodskaya, T. N. Krasnova

“…Genetic polymorphism testing was carried out by real-time polymerase chain reaction with melting curve analysis. Results. …”
Get full text

In vitro inhibition of Xanthomonas vasicola pv. musacearum, the causal agent of banana Xanthomonas Wilt, using medicinal plant extracts from North Kivu, Eastern Democratic Republic... by Franchement Mukeshambala, Angele Ibanda, Ludivine Lassois, Gakuru Semacumu, Dhed’a Djailo, Leon Nabahungu, Guy Blomme, Godefroid Monde

“…Pure Xvm colonies were used for identification via i) Polymerase Chain Reaction (PCR) with specific primers and, ii) greenhouse inoculation trials. …”
Get full text

PABPN1 functions as a downstream gene of CREB to inhibit the proliferation of preadipocytes by Xiao-Han Zhang, Jia-Xin Li, Xiao-Xu Wu, Qian Zhang, Ming Tian, Si-Qi Yang, Di Liu, Xiu-Qin Yang

“…Preadipocyte proliferation was measured with cell counting kit-8, 5-ethynyl-2′-deoxyuridine, real-time quantitative polymerase chain reaction, Western blotting, and flow cytometry analyses. …”
Get full text

Carbapenem resistance in gram-negative pathogens in an Iranian hospital: high prevalence of OXA-type carbapenemase genes by Setareh Mamishi, Reihaneh Hosseinpour Sadeghi, Sadaf Sajedi Moghaddam, Babak Pourakbari, Shiva Poormohammadi, Maryam Sotoudeh Anvari, Shima Mahmoudi

“…Antibiotic susceptibility testing was performed according to Clinical and Laboratory Standards Institute. Polymerase chain reaction was used to detect carbapenem resistance genes including bla OXA23, bla OXA24, bla OXA48, bla OXA51, bla OXA58, bla OXA143, bla KPC, bla IMP, bla VIM, and bla NDM. …”
Get full text

lncRNA SNHG15 as a ceRNA modulates Osteoclast Differentiation, Proliferation, and Metastasis by Sponging miR-381-3p/NEK2 Axis by YiFan Wang, GuanYin Zhu, Fang Pei, ZhiHe Zhao

“…The expressions of SNHG15, miR-381-3p, and never in mitosis-related kinase 2 (NEK2) mRNA were detected by real-time quantitative polymerase chain reaction (RT-qPCR); Western blot detected NEK2 and osteoclast markers (Cathepsin K, CTSK), matrix metalloproteinase 9 (MMP9), nuclear factor of activated T cell 2 (NFAT2), and tartrate-resistant acid phosphatase (TRAP) protein levels; cell proliferation was detected by Cell Counting Kit-8 (CCK-8), and the formation of osteoclasts was observed by TRAP staining; the F-actin skeleton was stained with tetramethylrhodamine isothiocyanate (TRITC) phalloidin; cell migration rate was detected by Transwell; dual-luciferase reporter gene assay and RNA-binding protein immunoprecipitation (RIP) assay verified the targeting relationship between miR-381-3p, SNHG15, and NEK2. …”
Get full text

Evaluation of ovarian stiffness and its biological mechanism using shear wave elastography in polycystic ovary syndrome by Yifang He, Shuangping Deng, Yanli Wang, Xiali Wang, Qingqing Huang, Jing Cheng, Dandan Wang, Guorong Lyu

“…Histological analysis, enzyme-linked immunosorbent assay, quantitative reverse transcription-polymerase chain reaction, western blotting, transcriptomics, and proteomics were performed to assess alterations in fibrosis and basement membrane (BM) gene expression. …”
Get full text

Effects of residential acaricide treatments on patterns of pathogen coinfection in blacklegged ticks by Richard S. Ostfeld, Sahar Adish, Stacy Mowry, William Bremer, Shannon Duerr, Andrew S. Evans, Ilya R. Fischhoff, Fiona Keating, Jennifer Pendleton, Ashley Pfister, Marissa Teator, Felicia Keesing, Ala Tabor

“…Quantitative polymerase chain reaction was used for pathogen detection in multiplex reactions. …”
Get full text

Calcium Activation of the Androgen Receptor in Prostate Cells by Zeina W. Sharawi, Sawsan M. Khatrawi, Qiaochu Wang, Hongzhao Zhou, Kedra Cyrus, Gai Yan, Becky Hoxter, Bassem R. Haddad, Mary Beth Martin

“…The expression of androgen-responsive genes and calcium channels was measured in prostate cells using a quantitative real-time polymerase chain reaction assay. Cell growth was measured. …”
Get full text

Toll-like receptor upregulation in liver and peripheral blood mononuclear cells of patients with amoebic liver abscess by Sandhya Khunger, Abhishek Mewara, Upninder Kaur, Ajay Duseja, Pallab Ray, Naveen Kalra, Navneet Sharma, Rakesh Sehgal

“…Blood samples from 100 ALA and 41 healthy individuals were collected. mRNA expression of TLR 1 to 9 genes was measured using reverse transcriptase polymerase chain reaction (RT-PCR). Serum cytokines level was quantified by flow cytometry. …”
Get full text

Using diagnostic data from veterinary diagnostic laboratories to unravel macroepidemiological aspects of porcine circoviruses 2 and 3 in the United States from 2002-2023. by Guilherme Cezar, Edison Magalhães, Kinath Rupasinghe, Srijita Chandra, Gustavo Silva, Marcelo Almeida, Bret Crim, Eric Burrough, Phillip Gauger, Christopher Siepker, Marta Mainenti, Michael Zeller, Eduardo Fano, Pablo Piñeyro, Rodger Main, Mary Thurn, Paulo Lages, Cesar Corzo, Albert Rovira, Hemant Naikare, Rob McGaughey, Franco Matias-Ferreyra, Jamie Retallick, Jordan Gebhardt, Jon Greseth, Darren Kersey, Travis Clement, Angela Pillatzki, Jane Christopher-Hennings, Melanie Prarat, Ashley Johnson, Dennis Summers, Craig Bowen, Joseph Boyle, Kenitra Hendrix, Andreia G Arruda, Daniel Linhares, Giovani Trevisan

“…The data comprised of polymerase chain reaction (PCR) test results, sample type, and age group for PCV2 and PCV3 submissions from 2002-2023. …”
Get full text