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961
Uncovering genetic determinants of antioxidant properties in Thai landrace rice through genome-wide association analysis
Published 2025-01-01“…Two genetic markers for polymerase chain reaction detection were developed for the OsTT8 gene and the OsRc promoter, both of which were positively correlated with antioxidant properties. …”
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962
Effects of Adipose-tissue Derived Mesenchymal Stem Cells on PBMCs in Co-culture with HeLa Cell Line
Published 2024-04-01“…Materials and Methods: The effect of MSC cells on proliferation, apoptosis, and gene expression of the cytokines tumor necrosis factor alpha (TNF-α), transforming growth factor beta (TGF-β), interleukin (IL)-4, IL-2, and interferon-γ in PBMCs cultured together with HeLa cells was investigated using flow cytometry and real-time polymerase chain reaction (PCR), respectively. Results: Flow cytometry showed that co-culture of PBMCs/MSCs/HeLa significantly increased the proliferation of PBMCs at different time points, with a p-value of 0.0022. …”
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963
Implications of cytokine genes polymorphisms in Jordanian patients with obsessive compulsive disorder
Published 2025-01-01“…Five polymorphic positions in four interleukin genes (IL-1β; rs16944 and rs1143634, IL-6; rs1800795, IL-10; rs1800896 and rs1800795) were genotyped using polymerase chain reaction–restriction fragment length polymorphism (PCR–RFLP) method. …”
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964
Newcastle disease virus genotype VII.1.1 identified from backyard chickens with low antibody titer: Jimma Zone, Southwest Ethiopia.
Published 2025-01-01“…From the swab samples, total RNA was extracted and the viral genomic material was detected by amplifying the Fusion gene by Reverse transcription polymerase chain reaction (RT-PCR). The interconnection between ELISA and RT-PCR was also analyzed. …”
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965
The Differential Effects of Leukocyte-Containing and Pure Platelet-Rich Plasma on Nucleus Pulposus-Derived Mesenchymal Stem Cells: Implications for the Clinical Treatment of Interv...
Published 2018-01-01“…NPMSCs isolated from rabbit NP tissues were treated with L-PRP or P-PRP in vitro, and then cell proliferation and expression of stem cell markers, proinflammatory cytokines (TNF-α, IL-1β), production of ECM (extracellular matrix-related protein), and NF-κB p65 protein were validated by CCK-8 assay, real-time polymerase chain reaction, enzyme-linked immunosorbent assay, immunofluorescence, and western blot respectively. …”
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966
The MADS-Box Transcription Factor ClAG2 Is a Key Regulator for the Formation of Double Flower in <i>Clematis</i> L.
Published 2024-12-01“…Quantitative real-time polymerase chain reaction (qRT-PCR) analysis revealed tissue-specific expression patterns among these genes. …”
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967
Utility of Integrated Analysis of Pharmacogenomics and Pharmacometabolomics in Early Phase Clinical Trial: A Case Study of a New Molecular Entity
Published 2018-09-01“…The pharmacogenomics screening was performed from whole blood samples using the Affymetrix DMET (Drug-Metabolizing Enzymes and Transporters) Plus microarray, and confirmation of genetic variants was performed using real-time polymerase chain reaction. Metabolomics profiling was performed from plasma samples using liquid chromatography coupled with quadrupole time-of-flight mass spectrometry. …”
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968
The Association between Deoxyribonucleic Acid Hypermethylation in Intron VII and Human Leukocyte Antigen-C∗07 Expression in Patients with Endometriosis
Published 2023-01-01“…The differential DNA methylation of the selected genes was validated via direct bisulfite sequencing and analysis of their messenger ribonucleic acid (mRNA) levels using quantitative reverse transcription polymerase chain reaction (qRT-PCR). Results. Fifteen differentially methylated CpG sites were found among the patients and healthy women, and five CpG sites were mapped to the introns of the human leukocyte antigen-C (HLA-C) gene; these were highly polymorphic between different HLA-C alleles and were HLA-C∗07 specific. …”
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969
miR-576-5p Promotes the Proliferation of Papillary Thyroid Carcinoma through the MAPK4-AKT Pathway
Published 2022-01-01“…Expression levels of miR-576-5p in PTC patient tissues and cell lines were determined by reverse transcription-quantitative polymerase chain reaction (qRT‒PCR). Cell counting using cell counting kit-8 (CCK-8), wound healing, and Transwell assays were performed to evaluate the effect of miR-576-5p on the proliferation, migration, and invasion of TPC-1 cells. …”
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970
Are there benefits of culture-based detection of Mycobacterium avium spp paratuberculosis over histopathology?
Published 2025-02-01“…Deoxyribonucleic acid (DNA) extracted from culture isolates was subjected to polymerase chain reaction (PCR) amplification using primers that target the IS900 regions of the MAP for species verification. …”
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971
The clinical role of blood coagulation and platelet receptors gene allelic variants in development of Wilson’s disease
Published 2018-08-01“…Genetic polymorphism testing was carried out by real-time polymerase chain reaction with melting curve analysis. Results. …”
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972
In vitro inhibition of Xanthomonas vasicola pv. musacearum, the causal agent of banana Xanthomonas Wilt, using medicinal plant extracts from North Kivu, Eastern Democratic Republic...
Published 2024-10-01“…Pure Xvm colonies were used for identification via i) Polymerase Chain Reaction (PCR) with specific primers and, ii) greenhouse inoculation trials. …”
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973
PABPN1 functions as a downstream gene of CREB to inhibit the proliferation of preadipocytes
Published 2025-01-01“…Preadipocyte proliferation was measured with cell counting kit-8, 5-ethynyl-2′-deoxyuridine, real-time quantitative polymerase chain reaction, Western blotting, and flow cytometry analyses. …”
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974
Carbapenem resistance in gram-negative pathogens in an Iranian hospital: high prevalence of OXA-type carbapenemase genes
Published 2025-01-01“…Antibiotic susceptibility testing was performed according to Clinical and Laboratory Standards Institute. Polymerase chain reaction was used to detect carbapenem resistance genes including bla OXA23, bla OXA24, bla OXA48, bla OXA51, bla OXA58, bla OXA143, bla KPC, bla IMP, bla VIM, and bla NDM. …”
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975
lncRNA SNHG15 as a ceRNA modulates Osteoclast Differentiation, Proliferation, and Metastasis by Sponging miR-381-3p/NEK2 Axis
Published 2022-01-01“…The expressions of SNHG15, miR-381-3p, and never in mitosis-related kinase 2 (NEK2) mRNA were detected by real-time quantitative polymerase chain reaction (RT-qPCR); Western blot detected NEK2 and osteoclast markers (Cathepsin K, CTSK), matrix metalloproteinase 9 (MMP9), nuclear factor of activated T cell 2 (NFAT2), and tartrate-resistant acid phosphatase (TRAP) protein levels; cell proliferation was detected by Cell Counting Kit-8 (CCK-8), and the formation of osteoclasts was observed by TRAP staining; the F-actin skeleton was stained with tetramethylrhodamine isothiocyanate (TRITC) phalloidin; cell migration rate was detected by Transwell; dual-luciferase reporter gene assay and RNA-binding protein immunoprecipitation (RIP) assay verified the targeting relationship between miR-381-3p, SNHG15, and NEK2. …”
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976
Evaluation of ovarian stiffness and its biological mechanism using shear wave elastography in polycystic ovary syndrome
Published 2025-01-01“…Histological analysis, enzyme-linked immunosorbent assay, quantitative reverse transcription-polymerase chain reaction, western blotting, transcriptomics, and proteomics were performed to assess alterations in fibrosis and basement membrane (BM) gene expression. …”
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977
Effects of residential acaricide treatments on patterns of pathogen coinfection in blacklegged ticks
Published 2024-08-01“…Quantitative polymerase chain reaction was used for pathogen detection in multiplex reactions. …”
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978
Calcium Activation of the Androgen Receptor in Prostate Cells
Published 2023-01-01“…The expression of androgen-responsive genes and calcium channels was measured in prostate cells using a quantitative real-time polymerase chain reaction assay. Cell growth was measured. …”
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979
Toll-like receptor upregulation in liver and peripheral blood mononuclear cells of patients with amoebic liver abscess
Published 2025-03-01“…Blood samples from 100 ALA and 41 healthy individuals were collected. mRNA expression of TLR 1 to 9 genes was measured using reverse transcriptase polymerase chain reaction (RT-PCR). Serum cytokines level was quantified by flow cytometry. …”
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980
Using diagnostic data from veterinary diagnostic laboratories to unravel macroepidemiological aspects of porcine circoviruses 2 and 3 in the United States from 2002-2023.
Published 2024-01-01“…The data comprised of polymerase chain reaction (PCR) test results, sample type, and age group for PCV2 and PCV3 submissions from 2002-2023. …”
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