Showing 941 - 960 results of 1,349 for search '"Polymerase chain reaction"', query time: 0.06s Refine Results
  1. 941

    TERT de novo mutation-associated dyskeratosis congenita and porto-sinusoidal vascular disease: a case report by Ge Yu, Guijie Xin, Xu Liu, Wanyu Li, Chen Shao, Runping Gao

    Published 2025-01-01
    “…Whole exome sequencing identified a heterozygous TERT c.2286 + 1G > A de novo mutation and quantitative polymerase chain reaction revealed very short telomeres (less than the first percentile for his age). …”
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  2. 942

    Palmitic acid-induced cell death: impact of endoplasmic reticulum and oxidative stress, mitigated by L-citrulline by Md. Rezwanul Habib, Yukako Tokutake, Shinichi Yonekura

    Published 2025-01-01
    “…Real-time quantitative polymerase chain reaction was used to explore the regulation of genes associated with oxidative stress, and ER stress genes. …”
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  3. 943

    Identification of Survival Risk and Immune-Related Characteristics of Kidney Renal Clear Cell Carcinoma by Xiaobin Wu, Yonghui Liang, Xian Chen, Xiangyang Long, Wujun Xu, Li Liu, Binhui Wang, Xiong Zou

    Published 2022-01-01
    “…Last but not the least, the expressions of some genes used to construct IGRPM in early KIRC, and adjacent normal tissues were verified through real-time fluorescence quantitative polymerase chain reaction (RT-qPCR). Perl (strawberry-perl-5.30.0.1-64bit), R software (4.0.3), and GraphPad Prism 7 were used to process the relevant data. …”
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  4. 944

    Possibility of Using NO Modulators for Pharmacocorrection of Endothelial Dysfunction After Prenatal Hypoxia by Igor Belenichev, Olena Popazova, Oleh Yadlovskyi, Nina Bukhtiyarova, Victor Ryzhenko, Sergii Pavlov, Valentyn Oksenych, Oleksandr Kamyshnyi

    Published 2025-01-01
    “…The expression of VEGF and VEGF-B mRNA was analyzed via real-time polymerase chain reaction, and the nuclear area of myocardial microvessel endothelial cells was evaluated morphometrically. …”
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  5. 945

    Molecular Detection and Genetic Characterization of Avian Leukosis Virus From Field Outbreaks in Bangladesh by Md. Golzar Hossain, Riman Pathan, SM Nazmul Hasan, Anandha Mozumder, Moslema Jahan Mou, Marjana Akter, Chandan Sikder, Riyan Al Islam Reshad, Roni Mia, Sukumar Saha, Tofazzal Islam, Sharmin Akter

    Published 2024-11-01
    “…The diagnosis of ALV infection in chickens was confirmed through necropsy, histopathological examinations, reverse transcription‐polymerase chain reaction (RT‐PCR), and sequence analysis. …”
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  6. 946
  7. 947

    Downregulation of LPAR1 Promotes Invasive Behavior in Papillary Thyroid Carcinoma Cells by Zahra Bokaii Hosseini, Fatemeh Rajabi, Reza Morovatshoar, Mahshad Ashrafpour, Panteha Behboodi, Dorsa Zareie, Mohammad Natami

    Published 2024-09-01
    “…The levels of LPAR1 expression were then measured using quantitative real-time polymerase chain reaction (qRT-PCR) in the BCPAP cell line, with a comparison to the Nthy-ori 3-1 cell line. …”
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  8. 948

    Enucleated bone marrow-derived mesenchymal stromal cells regulate immune microenvironment and promote testosterone production through efferocytosis by Lu Sun, Jiayu Huang, Xuezi Wang, Peng Huang, Baolin Dong, Zehang Liang, Jiahong Wu, Jiancheng Wang

    Published 2025-02-01
    “…To determine the mechanism of efferocytosis, we analysed the number of macrophages by immunofluorescence staining and quantitative real-time polymerase chain reaction (qRT-PCR). Results The injection of enucleated BMSCs (Cargocytes) into the testes of EDS-treated rats restored the levels of serum testosterone, increased the number of Leydig cells (LCs), and improved spermatogenesis. …”
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  9. 949

    Decreased expression of miR-200a and miR-223-3p in endometriosis during the secretory phase of menstrual cycle: Insights from a case-control study on molecular biomarkers and disea... by Yasaman Nazari Hagh, Mohamadreza Ahmadifard, Sedigheh Esmaelzadeh, Soheila Abbaszadeh, Naser Shokrzadeh

    Published 2025-02-01
    “…Histopathological examination (hematoxylin and eosin and periodic acid schiff) was performed to confirm the secretory stage, and miR-200a and miR-223-3p expressions were analyzed by quantitative reverse transcriptase-polymerase chain reaction. Results: Histological analysis confirmed that both groups were in the secretory stage. …”
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  10. 950

    Topical transdermal administration of lenalidomide nanosuspensions-based hydrogels against melanoma: In vitro and in vivo studies by Mengdi Zhang, Haiying Qiu, Zheyi Han, Yazhong Ma, Jingjing Hou, Jingwei Yuan, Haiyan Jia, Menglu Zhou, Hongjie Lu, Yan Wu

    Published 2025-06-01
    “…The effects of lenalidomide on melanoma were further verified through Western blotting, immunohistochemistry, immunofluorescence, and quantitative real-time polymerase chain reaction,using both in vitro cell experiments and in vivo melanoma mouse models. …”
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  11. 951

    Cell-free DNA release following psychosocial and physical stress in women and men by A. S. Limberg, F. Berg, E. Köper, C. Lindgraf, C. Gevers, R. Kumsta, E. M. Hummel, D. A. Moser

    Published 2025-01-01
    “…To overcome this, a multiplex quantitative polymerase chain reaction (qPCR) protocol targeting L1PA2 elements, which are prevalent in the human genome, was utilized. …”
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  12. 952

    Frequency of IL-1B Gene Polymorphisms in Patients with Gastroesophageal Cancer in the Hakkari Region by Derya Yaman, Selin Akad-Dinçer, Yusuf Karakaş, Gözde Ünsoy, Yunus Kasım Terzi, Feride İffet Şahin

    Published 2024-01-01
    “….-598C>T, and rs1143634 c.315C>T polymorphisms in the IL-1B gene were studied among groups via polymerase chain reaction and restriction fragment length polymorphism. …”
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  13. 953

    Comparison of miRNA expressions among benign, premalignant and malignant lesions of the larynx: could they be transformation biomarkers? by Fatma Ruya Tuncturk, Ibrahim Akalin, Lokman Uzun, Tulay Zenginkinet

    Published 2021-02-01
    “…Materials and methods We investigated mature miRNA expressions in paraffin-embedded surgical specimens of human laryngeal tissues grouped as benign, premalignant or malignant (n = 10 in each). miRNA profiling was carried out by quantitative Real-Time polymerase chain reaction (RT-qPCR) and data were analyzed according to fold regulation. …”
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  14. 954

    Metagenomics: a new frontier for routine pathology testing of gastrointestinal pathogens by Nicola Z. Angel, Mitchell J. Sullivan, Areej Alsheikh-Hussain, Liang Fang, Samantha MacDonald, Alena Pribyl, Blake Wills, Gene W. Tyson, Philip Hugenholtz, Donovan H. Parks, Paul Griffin, David L. A. Wood

    Published 2025-01-01
    “…This study aims to determine the ability to routinely apply mNGS testing, in comparison to traditional culture or polymerase chain reaction (PCR) based tests, for the identification of causal pathogens for gastrointestinal infections. …”
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  15. 955

    Occurrence of Enteric Viruses in Surface Water and the Relationship with Changes in Season and Physical Water Quality Dynamics by Wasonga Michael Opere, Maingi John, Omwoyo Ombori

    Published 2020-01-01
    “…Physical quality parameters were measured on-site while viral analysis was carried out by molecular methods using the nested polymerase chain reaction (nPCR). From 216 samples that were analysed for viral contamination, enteric viral genomes were discovered in 18 (8.3%) of the samples. …”
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  16. 956

    Molecular biological research and molecular homologous modeling of Bw.03 subgroup by WANG Li, KONG Yongkui, JIN Huifang, LIU Xin, XIE Ying, LIU Xue, CHANG Yanli, WANG Yafang, YANG Shumiao, ZHU Di, YANG Qiankun

    Published 2025-01-01
    “…[Methods] The ABO phenotype was identified by the tube method and microcolumn gel method; the ABO gene of the proband was detected by sequence-specific primer polymerase chain reaction (PCR-SSP), and the exon 6 and 7 of the ABO gene were sequenced and analyzed. …”
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  17. 957

    Activity of ceftolozane/tazobactam and comparators against gram-negative bacilli: Results from the Study for Monitoring Antimicrobial Resistance Trends (SMART – Brazil), 2018‒2021... by Amanda Azevedo Bittencourt, Vinicius Lima Faustino, Paula de Mendonça Batista, Lays Paulino Leonel, Marina Della Negra de Paula, Thales José Polis

    Published 2025-01-01
    “…Antimicrobial susceptibility testing and molecular characterization of ß-lactamase encoding genes using Polymerase Chain Reaction (PCR) and DNA sequencing were carried out from GNB isolated mostly from intra-abdominal, respiratory, and urinary tract infections and interpreted following BrCAST/EUCAST guidelines. …”
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  18. 958

    Effects on maternal and pregnancy outcomes of first-trimester malaria infection among nulliparous women from Kenya, Zambia, and the Democratic Republic of the Congo. by Sequoia I Leuba, Daniel Westreich, Carl L Bose, Andrew F Olshan, Steve M Taylor, Antoinette Tshefu, Adrien Lokangaka, Waldemar A Carlo, Elwyn Chomba, Musaku Mwenechanya, Edward A Liechty, Sherri L Bucher, Osayame A Ekhaguere, Fabian Esamai, Paul Nyongesa, Saleem Jessani, Sarah Saleem, Robert L Goldenberg, Janet L Moore, Tracy L Nolen, Jennifer Hemingway-Foday, Elizabeth M McClure, Marion Koso-Thomas, Richard J Derman, Matthew Hoffman, Steven R Meshnick, Melissa Bauserman

    Published 2024-01-01
    “…<h4>Methods</h4>In a convenience sample of women from the ASPIRIN (Aspirin Supplementation for Pregnancy Indicated risk Reduction In Nulliparas) trial in Kenya, Zambia, and the Democratic Republic of the Congo, we tested for first-trimester Plasmodium falciparum infection using quantitative polymerase chain reaction. We estimated site-specific effects on pregnancy outcomes using parametric g-computation.…”
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  19. 959

    Effects of Cranberry Extract (Vaccinium macrocarpon) Supplementation on Lipid Peroxidation and Inflammation in Patients with Chronic Kidney Disease (Stages 3-4): A Randomized Contr... by Laís de Souza Gouveia Moreira, Karla Thaís Resende Teixeira, Ludmila F. M. F. Cardozo, Livia Alvarenga, Bruna Regis, Jessyca Sousa de Brito, Viviane de Oliveira Leal, Natalia Alvarenga Borges, Isabela de Souza da Costa Brum, José Carlos Carraro-Eduardo, Giovanna B. Borini, Andresa A. Berretta, Marcelo Ribeiro-Alves, Denise Mafra

    Published 2024-01-01
    “…The mRNA expression of nuclear factor-erythroid 2-related factor-2 (Nrf2) and nuclear factor-kB (NF-kB) was evaluated in peripheral blood mononuclear cells (PBMCs) by quantitative real-time polymerase chain reaction. Thiobarbituric acid reactive substances (TBARS) were measured in the plasma to assess oxidative stress. …”
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  20. 960

    Shared Genes and Pathways in Ulcerative Colitis and Ankylosing Spondylitis: Functional Validation and Implications for Diagnosis by Li L, An G, Li F, Zhang D, Zhu X, Liang C, Zhao Y, Xie K, Zhou P, Zhu H, Jin X, Du L

    Published 2025-02-01
    “…Finally, peripheral blood samples of AS and UC were used to verify the mRNA expression of the eight key genes using reverse transcription-polymerase chain reaction (RT-PCR).Results: Our results revealed that GMFG, GNG11, CLEC4D, CMTM2, VAMP5, S100A8, S100A12 and DGKQ are potential diagnostic biomarkers of AS and UC. …”
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