Prevalence of the Helicobacter pylori babA2 Gene in Children Mainly Depends on the PCR Primer Set Used by Anja Šterbenc, Maja M. Lunar, Matjaž Homan, Boštjan Luzar, Nina Zidar, Mario Poljak

“…Various polymerase chain reaction- (PCR-) based methods with varying positivity rates were designed to detect the Helicobacter pylori babA2 gene. …”
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Prognostic values of intracellular cell-related genes in esophageal cancer and their regulatory mechanisms by Wei Cao, Dacheng Jin, Weirun Min, Haochi Li, Rong Wang, Jinlong Zhang, Yunjiu Gou

“…Established methodologies included morphological mapping, enrichment analysis, immune infiltration analysis, prognostic gene expression validation, molecular docking, and Reverse Transcription Polymerase Chain Reaction (RT-PCR) validation. Thirty-eight intersecting genes were identified between the disease and normal groups in ESCA samples. …”
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Association of leptin receptor Gln223Arg genetic polymorphism with obesity and non-alcoholic fatty liver disease by A. V. Morozova, N. V. Mal'tseva, Y. A. Gorbatovsky, O. F. Lykova, S. V. Arkhipova

“…Genotyping for LEPR Gln223Arg gene polymorphism was carried out by allele — specific polymerase chain reaction.Results. In control group and ALD patients no correlation of obesity with LEPR Gln223Arg gene polymorphism was revealed. …”
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Evaluating the Effect of Chloroform and Water Extract of Satureja khuzistanica on the Expression of Bcl-2 and Bax in Gastric Cancer Cell Line by Bagher Biglak, Asghar Tanomand, Leyla Açik, Farzad Arjomandi Rad, Gholamreza Shahsavari, Saman Kazemi, Hossein Soltanzadeh

“…The expression of Bax and Bcl-2 was evaluated using real-time polymerase chain reaction. Finally, all data were analyzed using REST software. …”
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Synergistic attenuation of complete freund’s adjuvant-induced inflammation in mice using shinbaro-pelubiprofen: a novel therapeutic complex by Hyunseong Kim, Jin Young Hong, Wan-Jin Jeon, Hyun Kim, Changhwan Yeo, Junseon Lee, Yoon Jae Lee, In-Hyuk Ha

“…Viability and cytotoxicity assays, immunohistochemistry, enzyme-linked immunosorbent assays, real-time polymerase chain reaction assays, nociception assays, writhing tests, and blood biochemical analyses were performed. …”
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Protective Effects of Necrostatin-1 against Concanavalin A-Induced Acute Hepatic Injury in Mice by Yingqun Zhou, Weiqi Dai, Chunlei Lin, Fan Wang, Lei He, Miao Shen, Ping Chen, Chenfen Wang, Jie Lu, Ling Xu, Xuanfu Xu, Chuanyong Guo

“…Levels of inflammatory cytokines with ConA-induced hepatitis were determined with real-time polymerase chain reaction (real-time PCR). The expression of TNF-α, RIP1, and LC3 was detected with immunohistochemical staining. …”
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Actin Alpha 2 (ACTA2) Downregulation Inhibits Neural Stem Cell Migration through Rho GTPase Activation by Ji Zhang, Xuheng Jiang, Chao Zhang, Jun Zhong, Xuanyu Fang, Huanhuan Li, Fangke Xie, Xiaofei Huang, Xiaojun Zhang, Quan Hu, Hongfei Ge, Anyong Yu

“…Here, primary NSCs were cultured and the expression of actin alpha 2 (ACTA2) in NSCs was determined using reverse transcription polymerase chain reaction (RT-PCR) and immunostaining. Next, the role of ACTA2 in regulating NSC migration and the potential mechanism was explored. …”
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Hypoglycemic effect and the mechanism of action of a polysaccharide from sweet corncob in a high-fat diet and streptozotocin-induced diabetic mice by Xin Wang, Weiye Xiu, Ye Han, Zhili Wang, Yu Luo, Yongqiang Ma

“…Interestingly, real time-polymerase chain reaction (RT-PCR) and Western blotting results revealed that SCP-80-I could regulate the expression of several glycolipid metabolisms and insulin secretion genes and proteins, including 5’-AMP-activated protein kinase (AMPK), carnitine palmitoyltransferase I (CPT1), and acetyl coenzyme A carboxylase (ACC) in the liver and AMPK, sirtuin 1 (Sirt1), peroxisome proliferator-activated receptorycoactivator-1 (PGC-1α), and uncoupling protein 2 (UCP2) in the pancreas. …”
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The expression and clinical significance of serum exosomal-long non-coding RNA DLEU1 in patients with cervical cancer by Yu Chen, Facai Cui, Xiaoyu Wu, Weifeng Zhao, Qingxin Xia

“…However, the potential clinical significance of DLEU1 in serum exosomes of patients with CC remains unclear.Methods The expression of serum exosomal DLEU1 was detected by quantitative real-time polymerase chain reaction (qRT-PCR). A receiver operating characteristic (ROC) curve was plotted to evaluate the clinical diagnostic efficacy of DLEU1. …”
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Regulatory factor X‐5/SCL/TAL1 interruption site axis promotes aerobic glycolysis and hepatocellular carcinoma cell stemness by Zhi‐Zhong Zhang, Zi‐Ming Wang, Hao‐Wen Zhang, Yan‐Xin Gong, Hao‐Ran Sun, Wei Zhang

“…Quantitative real‐time polymerase chain reaction was utilized to measure the STIL and RFX5 expression levels in HCC cells. …”
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METTL3-dependent DLG2 inhibits the malignant progression of cervical cancer by inactivating the Hippo/YAP signaling by Mei Pu, Xia Xiao, Shasha Lv, Daqing Ran, Qian Huang, Mingming Zhou, Qirong Lei, Lingshuang Kong, Qing Zhang

“…Methods Quantitative reverse transcription polymerase chain reaction and western blotting assays were employed to detect RNA and protein expression, respectively. …”
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A Systematic Review of the Prevalence and Diagnostic Workup of PIK3CA Mutations in HR+/HER2– Metastatic Breast Cancer by Elizabeth J. Anderson, Lea E. Mollon, Joni L. Dean, Terri L. Warholak, Ayal Aizer, Emma A. Platt, Derek H. Tang, Lisa E. Davis

“…The median prevalence of PIK3CA mutation was 36% (range: 13.3% to 61.5%); identified testing approaches more commonly used tissue over liquid biopsies and primarily utilized next-generation sequencing (NGS), polymerase chain reaction (PCR), or Sanger sequencing. There was concordance and stability between tissues (range: 70.4% to 94%) based on limited data. …”
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Improving prenatal diagnosis with combined karyotyping, CNV-seq and QF-PCR: a comprehensive analysis of chromosomal abnormalities in high-risk pregnancies by Jia-pei Liu, Shan-Bing Wang, Li Luo, Ya-mei Guo

“…ObjectiveThis study aims to assess the diagnostic efficacy of a combined approach integrating chromosomal karyotyping, copy number variation sequencing (CNV-seq), and quantitative fluorescence polymerase chain reaction (QF-PCR) in detecting chromosomal abnormalities in high-risk pregnancies.MethodsThis retrospective study analyzed 617 high-risk pregnancies undergoing prenatal diagnosis from February 2023 to August 2024, with amniotic fluid samples concurrently analyzed using karyotyping, CNV-seq, and QF-PCR. …”
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SIRT5‐mediated HOXA5 desuccinylation inhibits ferroptosis to alleviate sepsis induced‐lung injury by Lei Wang, Heng Fan, Min Sun, Ji‐Hui Ye

“…HOXA5, ferroptosis suppressor protein 1 (FSP1), sirtuin 5 (SIRT5), and glutathione peroxidase 4 (GPX4) expressions were measured using western blotting and Real‐Time Quantitative Polymerase Chain Reaction (RT‐qPCR. Chromatin immunoprecipitation and luciferase reporter assays were performed to validate HOXA5 binding to the FSP1/GPX4 promoter, and regulation of SIRT5 on HOXA5 desuccinylation was confirmed through co‐immunoprecipitation. …”
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MATN3 Gene Polymorphism Is Associated with Osteoarthritis in Chinese Han Population: A Community-Based Case-Control Study by Jiaao Gu, Jiesheng Rong, Fulin Guan, Liying Jiang, Shuqing Tao, Guofa Guan, Tianzun Tao

“…After obtaining the DNA of case and control groups, genotypes of the MATN3 SNP6 were determined by polymerase chain reaction followed by restriction enzyme digestion. …”
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Effects of coal mine dust on lung function in rats by LIU Yang, LI Meng, LU Liyuan, WANG Ru, YANG He, ZHANG Huifang

“…The relative mRNA expressions of peroxisome proliferators-activated receptor gamma coactivator 1-alpha (PGC-1α) and mitochondrial transcription factor A (TFAM) were detected using real-time fluorescent quantitative polymerase chain reaction assay. The relative protein expressions of PGC-1α and TFAM were detected using Western blotting. …”
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Protective Effect of High-Intensity Interval Training (HIIT) and Moderate-Intensity Continuous Training (MICT) against Vascular Dysfunction in Hyperglycemic Rats by Nurul Paramita, Brilliant C. Puspasari, Randika Arrody, Neng T. Kartinah, Trinovita Andraini, Julfiana Mardatillah, Hardiyanti Rusli, Dewi I. S. Santoso

“…Quantitative real-time polymerase chain reaction (PCR) was used to measure the expression of the receptor for advanced glycation end-products (RAGE) and nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB). …”
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Genome wide landscaping of copy number variations for horse inter-breed variability by Nitesh Kumar Sharma, Prashant Singh, Bibek Saha, Anuradha Bhardwaj, Mir Asif Iquebal, Yash Pal, Varij Nayan, Sarika Jaiswal, Shiv Kumar Giri, Ram Avatar Legha, T. K. Bhattacharya, Dinesh Kumar, Anil Rai

“…Also, three random CNVRs were validated with real-time polymerase chain reaction. These findings will aid in the understanding the horse genome and serve as a preliminary foundation for future CNV association research with commercially significant equine traits. …”
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DNM3OS Enhances the Apoptosis and Senescence of Spermatogonia Associated with Nonobstructive Azoospermia by Providing miR-214-5p and Decreasing E2F2 Expression by Rui Hua, Qingjun Chu, Feiyan Guo, Qinjie Chen, Maocai Li, Xuan Zhou, Yongtong Zhu

“…Microarray assays were performed on samples of testicular biopsy tissue obtained from patients with NOA for the purpose of identifying differentially expressed lncRNAs and messenger RNA (mRNA) transcripts, and the results were verified by quantitative real-time polymerase chain reaction. Mouse-derived GC-1 spermatogonia (spg) cells undergoing treatment with Adriamycin (ADR) were used to investigate the biological functions of the selected lncRNAs in vitro. …”
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Insights into Omicron: Genomic Characterization and Inpatient Risk Assessment at Single Tertiary Hospital in Indonesia by Turbawaty DK, Komala DR, Andriyoko B

“…Data was collected retrospectively from medical records, SARS-CoV-2 reverse transcriptase polymerase chain reaction (RT-PCR) results, and WGS results of patients at Hasan Sadikin General Hospital Bandung from July to December 2022, who met the inclusion criteria. …”
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