Identification and genetic analysis of the recent Avian pox viruses from infected pigeons and chickens by Amira M. Helal, Osama Mahana, Ali Zanaty, Karim Selim, Mohamed. E. Taha, Mostafa M. Saleh, Mohamed I. Hassan, Mona S. Abdallah

“…Results The polymerase chain reaction (PCR) assay was able to detect APV in all the tested samples; 12 positive samples (6 chicken flocks and 6 pigeon flocks) were selected for DNA sequencing. …”
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Predominance of metallo-beta-lactamase blaVIM Genes in Clinical Isolates from Health Facilities in Nigeria: Role in Multidrug Resistance Surge by Etang UE, Moses AE, Akpan SS, Eyo AO, Moses EA

“…Identification, antibiotic susceptibility testing and phenotypic detection of MBL-producers were done using the VITEK®2 system, Kirby-Bauer disc diffusion and imipenem+EDTA CDT, respectively. Polymerase chain reaction was used to investigate the characteristics of MBL encoding genes: blaVIM, blaNDM and blaIMP. …”
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Evaluation of the intracellular lipid-lowering effect of polyphenols extract from highland barley in HepG2 cells by Yijun Yao, Zhifang Li, Bowen Qin, Xingrong Ju, Lifeng Wang

“…We also preliminary studied the lipid-lowering mechanism by Real-time fluorescent quantitative polymerase chain reaction (qPCR). The results indicated that highland barley extract contains many components with lipid-lowering effects, such as hyperoside and scoparone. …”
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RNA-Sequence Reveals the Regulatory Mechanism of miR-149 on Osteoblast Skeleton under Mechanical Tension by Yifan Wang, Guanyin Zhu, Fang Pei, Yigan Wang, Jun Liu, Caixia Lu, Zhihe Zhao

“…Real-time quantitative polymerase chain reaction (RT-qPCR) detected the expression of miR-149 and ARFGAP with SH3 domain, Ankyrin repeat, and Ph domain 3 (ASAP3) mRNA. …”
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Evaluation of TNF-α, IL-10 and IL-6 Cytokine Production and Their Correlation with Genotype Variants amongst Tuberculosis Patients and Their Household Contacts. by Lavanya Joshi, Meenakshi Ponnana, Ramya Sivangala, Lakshmi Kiran Chelluri, Prathiba Nallari, Sitaramaraju Penmetsa, Vijayalakshmi Valluri, Sumanlatha Gaddam

“…<h4>Methods</h4>The cytokine levels were estimated in serum by enzyme-linked immunosorbent assay (ELISA) and their polymorphisms were studied by amplification refractory mutation system polymerase chain reaction (ARMs PCR) in active pulmonary tuberculosis patients (APTB = 150), household contacts (HHC = 190), and healthy controls (HC = 150).…”
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Cryoablation combined with dual immune checkpoint blockade enhances antitumor efficacy in hepatocellular carcinoma model mice by Jun Gu, Zepeng Yu, Xiangxiang Tang, Wenying Chen, Xuedong Deng, Xiaoli Zhu

“…The numbers of infiltrating T lymphocytes were checked by immunohistochemistry, and the levels of T-cell-associated cytokines were detected by Quantitative real-time Polymerase Chain Reaction (qRT-PCR) assays and Enzyme-Linked Immunosorbent Assays (ELISA) assays.Results Cryo + ICB inhibited the growth of right untreated tumors, promoted tumor cell apoptosis, and prolonged the survival time of mice. …”
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Aberrant DNA Methylation of FTH-1 and SHOX2 Contributes to Lung Cancer Progression by Salar Tahmasbi, Armin Sadeghi, Habib Zarredar, Milad Asadi, Shahryar Hashemzadeh, Venus Zafari, Hamed Sabbagh-Jadid, Mortaza Raeisi

“…Then, the methylation status of FTH-1 and SHOX2 was evaluated using quantitative methylation specific polymerase chain reaction (PCR) (qMSP). We used GraphPad Prism version 6.00 program for statistical analysis. …”
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Downregulation of TNF-α/TNF-R1 Signals by AT-Lipoxin A4 May Be a Significant Mechanism of Attenuation in SAP-Associated Lung Injury by Suhui Yu, Jianming Xie, Yukai Xiang, Shengjie Dai, Dinglai Yu, Hongwei Sun, Bicheng Chen, Mengtao Zhou

“…We used western blot, polymerase chain reaction (PCR), and immunofluorescence to investigate the downregulation of TNF-α signals in cellular and animal models of SAP-associated lung injury following AT-Lipoxin A4 intervention. …”
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Occupationally Acquired American Cutaneous Leishmaniasis by Maria Edileuza Felinto de Brito, Maria Sandra Andrade, Éricka Lima de Almeida, Ângela Cristina Rapela Medeiros, Roberto Pereira Werkhäuser, Ana Isabele Freitas de Araújo, Sinval Pinto Brandão-Filho, Alzira Maria Paiva de Almeida, Eduardo Henrique Gomes Rodrigues

“…We report two occupationally acquired cases of American cutaneous leishmaniasis (ACL): one accidental laboratory autoinoculation by contaminated needlestick while handling an ACL lesion sample, and one acquired during field studies on bird biology. Polymerase chain reaction (PCR) assays of patient lesions were positive for Leishmania, subgenus Viannia. …”
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lncRNA-KCNQ1OT1: A Potential Target in Exosomes Derived from Adipose-Derived Stem Cells for the Treatment of Osteoporosis by Shan-zheng Wang, Jun Jia, Chang-hong Chen

“…The expression of miRNA-141-5p (miR-141-5p) and lnc-KCNQ1OT1 was evaluated by quantitative real-time polymerase chain reaction (qRT-PCR). The protein expression of cleaved-caspase-3, caspase-3, and Bax was determined by Western blot. …”
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Utility of Ascitic Fluid Adenosine Deaminase Levels in the Diagnosis of Tuberculous Peritonitis in General Medical Practice by Ayako Kumabe, Shuji Hatakeyama, Naoki Kanda, Yu Yamamoto, Masami Matsumura

“…Tuberculous peritonitis is difficult to diagnose due to its varying clinical features, in addition to the low yield on bacterial culture or polymerase chain reaction using ascitic fluid samples. This study aimed to investigate the sensitivity and specificity of elevated adenosine deaminase (ADA) levels as a diagnostic marker for tuberculous peritonitis. …”
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TSP-1-1223 A/G Polymorphism as a Potential Predictor of the Recurrence Risk of Bladder Cancer in a Chinese Population by Xiao Yang, Pengchao Li, Xuejian Yang, Chao Qin, Qiang Cao, Zhengdong Zhang, Meilin Wang, Hongzhou Cai, Jinbao Gu, Jun Tao, Min Gu, Qiang Lu, Changjun Yin

“…Quantitative real-time polymerase chain reaction was performed to determine TSP-1 mRNA expression. …”
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Watermelon Germplasm with Resistance to Whitefly-transmitted Viruses by Alex Luckew, Nebahat Sari, Swikriti Pandey, Ted McAvoy, Alvin M. Simmons, Geoffrey Meru, Cecilia McGregor

“…Leaves were collected 5 weeks after transplanting (WAT) in 2022 and at 7 WAT in 2023 to determine the viral loads of Cucurbit leaf crumple virus (CuLCrV), Cucurbit yellow stunting disorder virus (CYSDV), and Cucurbit chlorotic yellows virus (CCYV) via a quantitative polymerase chain reaction. The CuLCrV and CYSDV viral loads were higher in 2022 than those in 2023, whereas the CCYV load was higher in 2023. …”
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Downregulation of serum miR-30c-5p serves as a biomarker to predict disease onset and short-term prognosis in acute coronary syndrome patients by Bo Chang, Xiangfeng Zhang, Riliang Fang, Huibin Li, Youdan Zhou, Yakun Wang

“…The miR-30c-5p expression was evaluated by the Polymerase chain reaction (PCR). The predictive value of miR-30c-5p for ACS was assessed by Receiver Operating Characteristic (ROC) curve and multivariate logistic regression analysis. …”
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Prevalence of ESBL resistance genes and fecal carriage of multidrug-resistant Pseudomonas aeruginosa isolates from patients with chronic kidney disease at the Laquintinie Hospital... by Ballue S.T. Dadjo, Armelle T. Mbaveng, Michael F. Kengne, Ornella D. Tsobeng, Giresse D.T. Fonjou, Victor Kuete

“…Antibiotic susceptibility testing was carried out using the Kirby-Bauer diffusion method. extended-spectrum β-lactamase (ESBL) resistance genes were detected by the polymerase chain reaction (PCR) method. Results: The prevalence of fecal carriage of Pseudomonas aeruginosa was 9.17 % (n = 42/458), including twenty-nine (69.05 %) in patients with chronic kidney disease and thirteen (30.95 %) in patients without chronic kidney disease. …”
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Comparison of Chest Computed Tomography (CT) Imaging Patterns and Severity among COVID-19 Patients during the First and Fourth Waves in Addis Ababa, Ethiopia by Lensa Million Baharu, Amir Alwan, Seife Teferi Dellie, Tesfaye Kebede Legesse, Kibruyisfaw Weldeab Abore

“…An institution-based cross-sectional study was conducted on 200 polymerase chain reaction test confirmed COVID-19 patients who underwent chest CT scan imaging in two diagnostic centers in Addis Ababa city. …”
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Development of a diagnostic multivariable prediction model of a positive SARS-CoV-2 RT-PCR result in healthcare workers with suspected SARS-CoV-2 infection in hospital settings. by Sandra Liliana Valderrama-Beltrán, Juliana Cuervo-Rojas, Martín Rondón, Juan Sebastián Montealegre-Diaz, Juan David Vera, Samuel Martinez-Vernaza, Alejandra Bonilla, Camilo Molineros, Viviana Fierro, Atilio Moreno, Leidy Villalobos, Beatriz Ariza, Carlos Álvarez-Moreno

“…We developed a diagnostic multivariate model to predict positive reverse transcription polymerase chain reaction (RT-PCR) results in HCWs with suspected SARS-CoV-2 infection.…”
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Virological and Immunological Aspects of AIDS Pathogenesis by Brian Conway, Francisco J Diaz-Mitoma

“…In the authors' laboratories, a quantitative polymerase chain reaction assay for the evaluation of circulating proviral load has been developed. …”
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Grazing mortality as a controlling factor in the uncultured non-cyanobacterial diazotroph (Gamma A) around the Kuroshio region by T. Sato, T. Sato, T. Yamaguchi, K. Hidataka, S. Sogawa, T. Setou, T. Kodama, T. Kodama, T. Shiozaki, K. Takahashi

“…In this study, we investigated the diazotroph community structure using <i>nifH</i> amplicon sequencing and quantified the growth and microzooplankton grazing mortality rate of Gamma A using a combination of dilution experiments and quantitative polymerase chain reaction (PCR) in well-lit waters at the northern edge of the Kuroshio Current off the southern coast of Japan. …”
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Mycobacterium avium subsp. paratuberculosis in an Italian Cohort of Type 1 Diabetes Pediatric Patients by Maria Luisa Manca Bitti, Speranza Masala, Francesca Capasso, Novella Rapini, Simona Piccinini, Federica Angelini, Andrea Pierantozzi, Roberta Lidano, Silvia Pietrosanti, Daniela Paccagnini, Leonardo A. Sechi

“…MAP DNA was detected using IS900-specific polymerase chain reaction (PCR). The presence of antibodies towards a MAP antigen, heparin binding hemoagglutinin (HBHA), was detected by ELISA. …”
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