Clinical performance of real-time nanopore metagenomic sequencing for rapid identification of bacterial pathogens in cerebrospinal fluid: a pilot study by Yoon Hyun Sung, Yong Kuk Ju, Hak Jun Lee, Seung Min Park, Jin Woong Suh, Jeong Yeon Kim, Jang Wook Sohn, Young Kyung Yoon

“…We conducted a 16S rRNA amplicon sequencing using a nanopore-based platform, alongside routine polymerase chain reaction (PCR) testing or bacterial culture, to compare its clinical performance in pathogen detection on CSF samples. …”
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Circ-PDE1C/miR-766-3p/SGTB axis regulates the IL-1β-induced apoptosis, inflammation and oxidative stress in human chondrocytes by Lixia Gao, Tao He, Qingkui Hu, Yan Ma

“…Methods Circ-PDE1C, microRNA-766-3p (miR-766-3p) or Small Glutamine Rich Tetratricopeptide Repeat Co-Chaperone Beta (SGTB) expression was determined using reverse transcription-quantitative polymerase chain reaction (RT-qPCR). Cell counting kit-8 (CCK-8) assay and flow cytometry were used to analyze proliferation and apoptosis, respectively. …”
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Dissociação clinicorradiológica em lactente com tuberculose pulmonar: relato de caso by Débora Elisabeth Sales Vieira, Rafael José Souza Filho, Rebecca da Silva Sales Vieira, Ana Carolina Gonçalves Galeano Arco, Juliènne Martins Araújo, Clarissa Netto dos Reys Laia Franco Prillwitz, Christiane Mello Schmidt, Claudete Araújo Cardoso

“…The diagnosis of Mtb infection was confirmed by a polymerase chain reaction test (Xpert Ultra MTB-RIF®). The patient was discharged to complete the treatment - rifampicin-isoniazid-pyrazinamide in an outpatient clinic with good clinical outcome. …”
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VASN knockout induces myocardial fibrosis in mice by downregulating non-collagen fibers and promoting inflammation by Junming Sun, Siwei Yin, Qiurui Li, Jun Zhang, Xiaoping Guo, Na Yu, Bing Hu, Yiqiang Ouyang, Qiaojuan Huang, Min He, Min He, Min He

“…VASN-knockout mice showed typical pathological, imaging, and molecular features of MF upon hematoxylin and eosin staining, Masson staining, Sirius red staining, quantitative polymerase chain reaction (qPCR), immunohistochemistry-paraffin (IHC-P), and immunofluorescence analyses. …”
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Effects of Insulin-like Growth Factor 1 on the Maintenance of Cell Viability and Osteogenic Differentiation of Gingiva-Derived Mesenchymal Stem Cell Spheroids by Somyeong Hwa, Hyun-Jin Lee, Youngkyung Ko, Jun-Beom Park

“…Additionally, quantitative polymerase chain reaction (qPCR) analysis was performed to determine the expression of RUNX2 and COL1A1. …”
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AMPK Activation in TET2 Downregulated Leukemia Cells Upon Glutamine Limitation by Ahsen Merve BAYRAK, Burcu YUCEL

“…GLUL mRNA expression was investigated using quantitative reverse transcription polymerase chain reaction and protein levels were evaluated using immunoblotting. …”
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Genome-wide development of simple sequence repeat (SSR) markers at 2-Mb intervals in lotus (Nelumbo Adans.) by Fengluan Liu, Lin Xi, Naifeng Fu

“…Finally, the polymerase chain reaction (PCR) efficiency of several SSR markers was validated in 20 Asian temperate lotus, eight Asian tropical lotus, and one American lotus. …”
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Is International Travel an Emerging Issue on Transmission of Beijing Lineage Mycobacterium tuberculosis? by Pavithra S. Madamarandawala, Srinath Satyanarayana, Collins Timire, Aashifa Yaqoob, Dushantha Madegedara, Dhammika N. Magana-Arachchi

“…Fresh sputum samples were collected for culturing and conducted polymerase chain reaction using BL-specific primers. Among the 101 patients recruited, presence of BL strains could be ascertained in 94 patients of which 24 (26%; 95% CI: 18%–35%) had BL strains. …”
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Assessing species composition and insecticide resistance of Anopheles gambiae complex members in three coastal health districts of Côte d'Ivoire. by Jackson K I Kouamé, Constant V A Edi, Julien B Z Zahouli, Ruth M A Kouamé, Yves A K Kacou, Firmain N Yokoly, Constant G N Gbalegba, David Malone, Benjamin G Koudou

“…An. gambiae complex members and kdr 995F, kdr 995S and Ace-1 280S mutations were identified using polymerase chain reaction (PCR) technique. Overall, An. gambiae s.l. populations were primarily composed of Anopheles coluzzii (88.24%, n = 312), followed by Anopheles gambiae sensu stricto (7.56%) and hybrids (4.17%). …”
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Association of eNOS Gene Polymorphisms G894T and T-786C with Risk of Hepatorenal Syndrome by Yuksel Seckin, Ali Yigit, Elif Yesilada, Gonca Gulbay, Yasir Furkan Cagin, Harika Gozukara, Yılmaz Bılgıc, Oguzhan Yildirim, Yusuf Turkoz, Zeynep Aksungur

“…Polymorphisms were determined by polymerase chain reaction (PCR) and melting curve analysis. …”
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Senescence-related circRNA circHIF-1α is associated with pancreatic cancer progression by Hao Hua, Yazu Deng, Dijie Zheng, Changhao Wu, Liwen Chen, Binbin Shi, Zhiwei He, Chao Yu

“…Meanwhile, significantly upregulated expression of circHIF-1α in pancreatic cancer tissue detected by reverse transcription-polymerase chain reaction (RT-qPCR) and in situ hybridization (ISH). …”
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Molecular Detection and Multidrug Resistance of Shigella spp. Isolated from Wild Waterfowl and Migratory Birds in Bangladesh by Jarna Karmoker, Md. Saiful Islam, Md. Liton Rana, Md. Ashek Ullah, Fahim Haque Neloy, Nahian Muniath Oishy, Pritom Kumar Pramanik, Mahbubul Pratik Siddique, Sukumar Saha, Md. Tanvir Rahman

“…Shigella spp. were isolated and identified by culturing, staining, and biochemical tests followed by polymerase chain reaction (PCR). A disk diffusion assay was employed to investigate antibiotic phenotypes, while the resistance genes were detected by PCR. …”
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Identification of urothelial cancer-associated 1 (UCA1) as a diagnostic biomarker of pre-eclampsia via regulating microRNA-16 and its downstream signaling pathway by Ye-Hong Xv, Wei Gao, Liao-Jv Wang

“…Enzyme-linked immunosorbent assay (ELISA), real-time polymerase chain reaction (PCR), Western blot analysis, immunohistochemistry (IHC) assay, computational analysis, and luciferase assay were conducted to measure the differential expression of UCA1, miR-16, and PAPP A while establishing a signaling pathway of UCA1/miR-16/PAPP-A. …”
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E46K α-Synuclein Mutation Fails to Promote Neurite Outgrowth by Not Inducing Cdc42EP2 Expression, Unlike Wild-Type or A53T α-Synuclein in SK-N-SH Cells by Hyunja Jung, Seonghan Kim

“…We also analyzed the differential regulation of cell division control 42 effector protein 2 (Cdc42EP2) using real-time quantitative polymerase chain reaction and western blot analysis. Finally, to confirm the implication of neurite outgrowth, we knocked down Cdc42EP2 using small interfering RNA. …”
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Two Cases of Ectopic Hamartomatous Thymoma Masquerading as Sarcoma by Takahito Kondo, Yukiko Sato, Hiroko Tanaka, Toru Sasaki, Kazuyoshi Kawabata, Hiroki Mitani, Hiroyuki Yonekawa, Hirofumi Fukushima, Wataru Shimbashi

“…In these cases, fusion gene detection via polymerase chain reaction or fluorescence in situ hybridization was useful for differentiating EHT from synovial sarcoma. …”
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Investigating the Expression of c-Myc and BIRC5 in TRAIL and PBOX-15 treated Metastatic Prostate Cancer Cells by Corinne Kai Leng Chew, Hannah O'Toole, Daniela M Zisterer, Seema M. Nathwani, Jade K. Pollock

“…Methods RNA was extracted from treated LNCaP cells. cDNA was synthesized, and gene specific primers were subsequently used for amplification in a polymerase chain reaction (PCR) assay. Gene expression was determined using gel electrophoresis and visualised using G:BOX. …”
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Comparison of Perinatal, Newborn, and Audiometry Results of COVID-19 Pregnant Women by Mehmet Yilmaz, Şerif Aksin, Deniz Balsak, Fazil Avci, Osman Özdoğru, Bekir Helvacıoğlu, Mahmut Erdemoğlu, Yasemin Aboalhasan, Gülsüm Doğan

“…This retrospective, single-center cohort study was conducted with 65 polymerase chain reaction (PCR) positive pregnant women and newborns and 66 normal pregnant women and newborns who were admitted between January 2020–December 2021. …”
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Clinical Recommendations of the Russian Gastroenterological Association on Diagnosis and Treatment of Infectious Esophagitis by V. T. Ivashkin, N. D. Yuschuk, I. V. Maev, A. S. Trukhmanov, O. A. Storonova, A. A. Makushina, S. S. Pirogov, O. V. Zayratyants, O. P. Alekseeva, D. N. Andreyev, T. L. Lapina, A. V. Tkachev, Yu. P. Uspenskiy, A. A. Sheptulin

“…Selected incidents of infectious esophagitis require a histological, immunohistochemical examination or polymerase chain reaction-based diagnosis to verify the infectious agent. …”
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Occurrence and Determination of Antimicrobial Resistant Escherichia coli Isolates in Fish and Vegetables as Indicator Organism of Faecal Contamination in Dar es Salaam, Tanzania by Francis Mwanza, Erick Vitus Gabriel Komba, Dominic Mukama Kambarage

“…Enterobacterial repetitive intergenic consensus polymerase chain reaction (ERIC-PCR) typing method was used to generate fingerprints and determine the genetic relatedness of the E. coli isolates. …”
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Environmental Surveillance for SARS-CoV-2 in Two Restaurants from a Mid-scale City that Followed U.S. CDC Reopening Guidance by Hongwan Li, Sripriya Nannu Shankar, Chiran T. Witanachchi, John A. Lednicky, Julia C. Loeb, Md. Mahbubul Alam, Z. Hugh Fan, Karim Mohamed, Jessica A. Boyette, Arantzazu Eiguren-Fernandez, Chang-Yu Wu

“…The samples were analyzed by real-time reverse transcriptase-polymerase chain reaction (RT-PCR) for the presence of SARS-CoV-2 genomic RNA. …”
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