COVID-19 Infection Clinical Profile, Management, Outcome, and Antibody Response in Kidney Transplant Recipients: A Single Centre Experience by Sanjiv Jasuja, Gaurav Sagar, Anupam Bahl, Shalini Verma

“…The 67 KTRs with polymerase chain reaction (PCR) confirmed COVID-19 infection reported between 1 May 2020 and 31 December 2020; 61.2% of patients were hospitalized; and 20.9% needed ventilation. …”
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Validation of a one-step reverse transcription PCR detection method for Tobamovirus maculatessellati, in tomato (Solanum lycopersicum L.) and pepper (Capsicum annuum L.) by Chellappan Padmanabhan, Andrea Gilliard, Kai-Shu Ling, Yazmín Rivera

“…In this study, we report the validation of a previously described reverse transcription polymerase chain reaction (RT-PCR) assay that amplifies a 289 bp fragment of the coat protein coding region of ToMMV genome. …”
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The Impact of Oleuropein on Cisplatin-Induced Toxicity in Cochlear Cells in Relation to the Expression of Deoxyribonucleic Acid Damage-Associated Genes by Yüksel Olgun, Zekiye Altun, Merve Tütüncü, Selen Kum Özşengezer, Safiye Aktaş, Enis Alpin Güneri

“…The change in the expression of 84 genes associated with CCDP, OLE, and OLE–CDDP treatments that induced DNA damage was tested using the reverse transcription polymerase chain reaction array. Changes ≥3-fold were considered significant. …”
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African swine fever in the Primorsky Krai: disease situation and molecular and biological properties of the isolate recovered from a wild boar long bone by А. R. Shotin, A. S. Igolkin, Ali Mazloum, I. V. Shevchenko, N. S. Bardina, E. O. Morozova, А. А. Shevtsov

“…The ASFV genome was detected in blood samples collected from infected pigs on 5–8 days post infection by real-time polymerase chain reaction. Specific antibodies in blood samples were not detected. …”
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Oligomeric procyanidins combined with Parabacteroides distasonis ameliorate high-fat diet-induced atherosclerosis by regulating lipid metabolism, inflammation reaction and bile aci... by Mingjuan Xu, Cheng Lü, Yiqing Hu, Mo Zhang, Jinxin Shen, Chunyi Liu, Qun Lu, Rui Liu

“…Further, quantitative polymerase chain reaction (qPCR) analysis showed that PPC intervention facilitated reverse cholesterol transport by upregulating Srb1 expression; In addition, PPC intervention promoted BA synthesis from cholesterol in liver by upregulating Cyp7a1 expression via suppression of the farnesoid X receptor (FXR) pathway, thus exhibiting a significant serum cholesterol-lowering effect. …”
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Polymorphism of MTHFR C677T Gene and the Associations with the Severity of Essential Hypertension in Northern Chinese Population by Junli Song, Jie Hou, Qiang Zhao, Xuezhi Liu, Qian Guo, Donghong Yin, Yan Song, Xiaoxia Li, Shuyun Wang, Xinchun Wang, Jinju Duan

“…A total of 985 subjects were enrolled to analyze the polymorphisms of the MTHFR C677T gene by polymerase chain reaction (PCR). 306 people with essential hypertension were selected from 985 subjects to estimate the severity of hypertension by the ordinal multivariate logistical regression model. …”
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Viral nucleic acid load in the milk of lactating mothers with COVID-19 and the prognosis of infants by Mengkun Wang, Yifei Li, Jie Lei, Huiyi Jiang

“…Milk samples from each lactating mother were collected consecutively for 2–18 days and subjected to polymerase chain reaction (PCR) testing forSARS-CoV-2 RNA detection. …”
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Association of Angiotensin I Converting Enzyme Insertion/287 bp Deletion Polymorphisms and Proliferative Prostatic Diseases among Lebanese Men by Asmahan A. El Ezzi, Jordan M. Clawson, Mohammed A. El-Saidi, Wissam R. Zaidan, Abigail Kovash, Jeremy Orellana, AnnaKarina Thornock, Ruhul H. Kuddus

“…Blood DNA extracted from 69 control subjects, 69 subjects with clinically confirmed PCa, and 69 subjects with clinical BPH, all the subjects were aged 50 years or older, was subjected to the polymerase chain reaction. The PCR products were resolved in polyacrylamide gels to determine II, ID, and DD genotypes. …”
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Efficacy of a “Rescue” Ciprofloxacin-Based Regimen for Eradication of Helicobacter pylori Infection after Treatment Failures by Maria Pina Dore, Vincenza Tadeu, Bianca Are, Ida Mura, Giuseppe Fanciulli, Giovannino Massarelli, Andrea Piana

“…H. pylori status: established by histology, rapide urease test and polymerase chain reaction. Intervention: esomeprazole 20 mg, ciprofloxacin 500 mg, and metronidazole 500 mg, administered together before breakfast and dinner for 10 days. …”
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The Presence of Antineutrophil Cytoplasmic Antibodies and Antiphospholipid Antibodies in Patients with Severe Acute Respiratory Syndrome Coronavirus 2: A Case-Control Study among S... by Mohammed Shams Aldeen, Musa Logman Masaad, Ayman Azhary, Abdulomez Suliman, Mohammed Ahmed Alziber, Motasim MohammedAhmed, Farag Alla Mohamed Aman, Salahaldeen Ismail, Nadir Abuzeid, Abdualmoniem O. Musa, Samuel Tekle Mengistu, Mohammed Elfatih Hamida

“…To investigate this, we conducted a case-control study which included patients with a positive reverse transcription-polymerase chain reaction (RT-PCR) test of COVID-19 and a control group with negative COVID-19 PCR and antibody (IgG-IgM and IgA nucleoprotein) ELISA results. …”
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Prevalence and Antimicrobial Resistance of Escherichia coli in Chicken Meat and Edible Poultry Organs Collected from Retail Shops and Supermarkets of North Western Province in Sri... by R. A. S. S. Ranasinghe, D. A. Satharasinghe, P. S Anwarama, P. M. S. D. K. Parakatawella, L. J. P. A. P. Jayasooriya, R. M. S. B. K. Ranasinghe, R. P. V. J. Rajapakse, J. T. Y. Huat, Y. Rukayadi, Y. Nakaguchi, M. Nishibuchi, S. Radu, J. M. K. J. K. Premarathne

“…Molecular confirmation for the positive samples was done through polymerase chain reaction (PCR) using previously designed primers. …”
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Novel PITX2 Mutations including a Mutation Causing an Unusual Ophthalmic Phenotype of Axenfeld-Rieger Syndrome by Liqin Huang, Yong Meng, Xiangming Guo

“…All coding exons of PITX2 and FOXC1 were amplified by polymerase chain reaction (PCR) from genomic DNA and subjected to direct DNA sequencing. …”
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Basidiobolomycosis Simulating a Mycobacterium ulcerans Infection in a Togolese Rural Child by Bayaki Saka, Waguena Gnassingbe, Garba Mahamadou, Sefako Akakpo, Julienne Teclessou, Aurel Abilogun-Chokki, Abas Mouhari-Toure, Koussake Kombate, Palokinam Pitché

“…The diagnosis of Buruli ulcer was evoked first; the search for Mycobacterium ulcerans by polymerase chain reaction was negative. Histology test performed revealed hypodermic granulomatous inflammation with predominant macrophage and eosinophils. …”
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circHUWE1 Exerts an Oncogenic Role in Inducing DDP-Resistant NSCLC Progression Depending on the Regulation of miR-34a-5p/TNFAIP8 by Xueliang Yang, Quan Sun, Yongming Song, Wenli Li

“…The quantitative real-time polymerase chain reaction (RT-qPCR) and western blot analyses were used for examining circHUWE1, microRNA-34a-5p (miR-34a-5p), and tumor necrosis factor alpha-induced protein 8 (TNFAIP8). …”
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Prevalence and predictors of NTM in presumed/confirmed drug-resistant TB by E.T. Abbew, R. Laryea, F. Sorvor, Y.A. Poku, N. Lorent, D. Obiri-Yeboah, L. Lynen, L. Rigouts

“…NTM isolates were re-cultured for species identification using GenoType® Mycobacterium CM/AS line-probe assay, polymerase chain reaction, and Sanger sequencing targeting 16S rRNA and rpoB genes. …”
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lnc-MRGPRF-6:1 Promotes ox-LDL-Induced Macrophage Ferroptosis via Suppressing GPX4 by Zhihuan You, Xiaotian Ye, Meihua Jiang, Ning Gu, Caihong Liang

“…Then, transcriptome sequencing, literature searching, and following quantitative real-time polymerase chain reaction and western blot were implemented to explore specific signaling pathway in the process. …”
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17α-Ethynyl-androst-5-ene-3β,7β,17β-triol (HE3286) Is Neuroprotective and Reduces Motor Impairment and Neuroinflammation in a Murine MPTP Model of Parkinson’s Disease by Ferdinando Nicoletti, Ingrid Philippens, Paolo Fagone, Clarence N. Ahlem, Christopher L. Reading, James M. Frincke, Dominick L. Auci

“…HE3286 treatment significantly improved motor function compared to vehicle in the rotarod test (mean 58.2 sec versus 90.9 sec, P<0.0001), and reduced inflammatory mediator gene expression in the brain (inducible nitric oxide synthase, 20%, P=0.002; tumor necrosis factor α, 40%, P=0.038, and interleukin-1β, 33%, P=0.02) measured by reverse-transcriptase polymerase chain reaction. Brain tissue histopathology and immunohistochemistry showed that HE3286 treatment increased the numbers of tyrosine hydroxylase-positive cells by 17% compared to vehicle (P=0.003), and decreased the numbers of damaged neurons by 38% relative to vehicle (P=0.029). …”
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miR-106a Is Downregulated in Peripheral Blood Mononuclear Cells of Chronic Hepatitis B and Associated with Enhanced Levels of Interleukin-8 by Zhongsi Hong, Haiyu Hong, Jian Liu, Xiaobin Zheng, Mingxing Huang, Chunna Li, Jinyu Xia

“…Materials and Methods. miR-106a expression levels in PBMCs from 40 healthy controls and 56 CHB patients were analyzed by quantitative real-time polymerase chain reaction (qRT-PCR). The luciferase activity assays were used to determine whether miR-106a binds to 3′UTR of IL-8. miR-106a mimics and inhibitors were transfected into healthy PBMCs. …”
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Quantitative expression of estrogen, progesterone and human epidermal growth factor receptor-2 and their correlation with immunohistochemistry in breast cancer at Uganda Cancer Ins... by Henry Wannume, Nixon Niyonzima, Sam Kalungi, Julius Boniface Okuni, Tonny Okecha, Edward Kakungulu, Steven Mpungu Kiwuwa, Geoffrey Waiswa, Sylvester Kadhumbula, Monica Namayanja, Martin Nabwana, Jackson Orem

“…This study aimed to determine the quantitative expression of ER, PR and HER-2 using Immunohistochemistry and their correlation with quantitative baseline Ct values measured using Quantitative Polymerase Chain Reaction (PCR). This study also assessed the use of fresh breast tissue biopsies preserved in RNAlater solution in the quantitative detection of these receptors using PCR technique. …”
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Ultrasensitive detection and quantification of bovine Deltapapillomavirus in the semen of healthy horses by Anna Cutarelli, Francesca De Falco, Francesco Serpe, Simona Izzo, Giovanna Fusco, Cornel Catoi, Sante Roperto

“…In 34 (58.6%), bovine δPV DNA was detected and quantified using droplet digital polymerase chain reaction (ddPCR). Real time quantitative PCR (qPCR) was able to identify bovine δPV DNA in 5 samples (8.6%). …”
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