Inhibitory Effect of Flavonoid Extract of Lotus Leaf on Alcohol-Induced Gastric Injury by Antioxidant Capacity in Mice by Yi Chen, Qin Li, Zhiping Kuang, Xin Zhao, Ruokun Yi, Xiaowei He, Guangjing Feng

“…The quantitative polymerase chain reaction (qPCR) and Western blot results proved that FELL upregulated the mRNA and protein expressions of SOD1, SOD2, CAT, epidermal growth factor (EGF), vascular endothelial growth factor (VEGF), epidermal growth factor receptor (EGFR), neuronal nitric oxide synthase (nNOS), and endothelial nitric oxide synthase (eNOS) and downregulated the expression of inducible nitric oxide synthase (iNOS) in the gastric tissue of mice with gastric injury. …”
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6-Gingerol Normalizes the Expression of Biomarkers Related to Hypertension via PPARδ in HUVECs, HEK293, and Differentiated 3T3-L1 Cells by Yong-Jik Lee, Yoo-Na Jang, Yoon-Mi Han, Hyun-Min Kim, Hong Seog Seo

“…The antihypertensive effects and mechanism of 6-gingerol were identified through reverse transcription polymerase chain reaction (RT-PCR), western blotting, and immunocytochemical staining for biomarkers involved in hypertension in human umbilical vein endothelial cells (HUVECs), human embryonal kidney cells (HEK293 cells), and mouse preadipocytes (3T3-L1 cells). …”
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Anticancer Activity of Holothuria Leucospilota-Derived Saponin on MKN45 Gastric Cancer Cell via Upregulation of CDH1 Hub Gene by Khadijeh Sadegh, Amirhossein Ahmadi, Ahmad Shadi, Ahmad Ghasemi

“…The effects of HLBS on the expression of the two top-ranked GC hub genes were measured using real-time polymerase chain reaction. Comparisons between groups were performed using the ANOVA test by GraphPad Prism software at the statistically significant P-value <0.05.Results: HLBS with an IC50 concentration of 75 μg/ml at 48 hours resulted in a significant decrease in cell viability, clonogenic ability (46.63% reduction, P < 0.01), and migration of MKN45 cells (74.5% reduction, P < 0.01). …”
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Results of Routine Antenatal Screening for Cytomegalovirus at a Tertiary Center by Duygu TUGRUL ERSAK, Izzet OZGURLUK, Ayse Gulcin BASTEMUR, Esra GULEN YILDIZ, Selcan SINACI, Ezgi TURGUT, Ozgur KARA, Atakan TANACAN, Dilek SAHIN

“…CMV immunoglobulin M (IgM), CMV IgG, anti-IgG avidity test results, amniocentesis, CMV polymerase chain reaction (PCR), and the outcome of the babies were recorded. …”
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Isorhamnetin Inhibits Liver Fibrosis by Reducing Autophagy and Inhibiting Extracellular Matrix Formation via the TGF-β1/Smad3 and TGF-β1/p38 MAPK Pathways by Ning Liu, Jiao Feng, Xiya Lu, Zhilu Yao, Qing Liu, Yang Lv, Yuru Han, Jingfan Deng, Yingqun Zhou

“…Protein and mRNA expressions were assayed by western blotting, immunohistochemistry, and quantitative real-time polymerase chain reaction. Results. Isorhamnetin significantly inhibited liver fibrosis in both models, inhibiting hepatic stellate cell (HSC) activation, extracellular matrix (ECM) deposition, and autophagy. …”
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An association study of polymorphisms in the P2RX7 gene in an Iranian population with the risk of type 2 diabetes mellitus by Homa Noori, Majid Rajabian, Mahdi Majidpour, Mahboobeh Sabeti Akbar-Abad, Ramin Saravani

“…DNA extraction followed by tetra amplification refractory mutation system polymerase chain reaction (Tetra ARMS-PCR) as a genotyping method. …”
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Presence of Rickettsia Species in a Marginalized Area of Yucatan, Mexico by Gaspar Peniche-Lara, Bertha Jimenez-Delgadillo, Claudia Munoz-Zanzi, María Cárdenas-Marrufo, Carlos Pérez-Osorio, Juan Arias-León

“…Conventional polymerase chain reaction and sequencing were used to identify the DNA of Rickettsia. …”
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CLINICAL SIGNS AND TREATMENT OF NEW-ONSET BONE MARROW FAILURE ASSOCIATED SARS-COV-2 INFECTION IN CHILDREN : A SINGLE INSTITUTION PROSPECTIVE COHORT STUD by Mervat A M youssef, Ebtisam Shawky hmed, Dalia Tarik Kamal, khalid Elsayh, Mai A Abdelfatta, Hyam Hassan Mahran Mahran, Mostafa M Embaby

“…Blood samples were obtained from all patients to detect SARS-CoV-2 antibodies, and nasopharyngeal swabs were collected for reverse-transcription Polymerase Chain Reaction to detect  SARS-CoV-2 viruses.  According to the laboratory results,  patients were classified in to SARS-CoV-2 positive  antibodies and SARS-CoV-2  negative antibodies .  …”
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Romidepsin Promotes Osteogenic and Adipocytic Differentiation of Human Mesenchymal Stem Cells through Inhibition of Histondeacetylase Activity by Dalia Ali, Elna P. Chalisserry, Muthurangan Manikandan, Rimi Hamam, Musaad Alfayez, Moustapha Kassem, Abdullah Aldahmash, Nehad M. Alajez

“…The results of chromatin immunoprecipitation combined with quantitative polymerase chain reaction indicated a significant increase in H3K9Ac epigenetic markers in the promoter regions of peroxisome proliferator-activated receptor gamma (PPARγ) and KLF15 (related to adipogenesis) or SP7 (Osterix) and alkaline phosphatase (ALP) (related to osteogenesis) in romidepsin-treated BMSCs. …”
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Development of real-time RT-PCR for N2 subtype avian influenza RNA-virus detection by P. B. Akshalova, A. V. Andriyasov, L. O. Scherbakova, S. N. Kolosov, N. G. Zinyakov, I. A. Chvala, D. B. Andreychuk

“…Data on oligonucleotide primer selection and reverse transcription real-time polymerase chain reaction condition optimization for N2 AI virus detection are presented in the paper. …”
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Assessment of biofilm formation, antibiotic resistance patterns, and the prevalence of adhesion-related genes in clinical Staphylococcus aureus isolates by Nabi Jomehzadeh, Sogol Seif Emrani

“…Strains were identified via microbiological methods and polymerase chain reaction (PCR) to target the nuc gene. Antibiotic susceptibility testing (AST) was conducted via the disc diffusion method. …”
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NLRP3 is a BMI-independent mediator of stable COPD by Yonca Gungor, Selin Ercan, Saliha Selin Özuygur Ermiş, Yiğit Kozalı, Gizem Kursunluoglu, Ceyda Sahan, Aylin Ozgen Alpaydin, Hulya Ayar Kayali

“…Peripheral blood mononuclear cells were isolated, and total RNA was extracted for real-time quantitative polymerase chain reaction (qPCR) analysis to measure the expression levels of inflammasome molecules. …”
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Sorbitol Destroyed Intestinal Microfold Cells (M Cells) Development through Inhibition of PDE4-Mediated RANKL Expression by Li Xiang, Wenxu Pan, Huan Chen, Wenjun Du, Shuping Xie, Xinhua Liang, Fangying Yang, Rongwei Niu, Canxin Huang, Minan Luo, Yuxin Xu, Lanlan Geng, Sitang Gong, Wanfu Xu, Junhong Zhao

“…Materials and Methods. Real-time polymerase chain reaction, immunofluorescence, and western blotting were performed to analyze the effect of sorbitol-regulated M-cell differentiation in vivo and in vitro, and luciferase and chromatin Immunoprecipitation were used to reveal the mechanism through which sorbitol-modulated M-cell differentiation. …”
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Enhancing the color and stress tolerance of cherry shrimp (Neocaridina davidi var. red) using astaxanthin and Bidens Pilosa. by Wei-Wei Hou, Yu-Tzi Chang, Wen-Chin Yang, Hong-Yi Gong, Yen-Ju Pan, Te-Hua Hsu, Chang-Wen Huang

“…Color parameters were assessed using CIELAB color space, and differential gene expression related to color and stress was analyzed using reverse transcription quantitative polymerase chain reaction (RT-qPCR) to understand the gene regulatory mechanisms affecting color expression and stability. …”
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Comparative analysis of EGFR mutations in circulating tumor DNA and primary tumor tissues from lung cancer patients using BEAMing PCR by Duhita Mirikar, Nandini Banerjee, Kumar Prabhash, Rajiv Kumar Kaushal, Vanita Naronha, C. S. Pramesh, George Karimundackal, Amit Joshi, Swapnil Rane, Ranjan Basak

“…Abstract In this study, we measured human epidermal growth factor receptor (EGFR) mutations in both tissue and circulating tumor DNA (ctDNA) by using beads, emulsions, amplifications and magnetic polymerase chain reaction (BEAMing PCR). Noninvasive mutation detection by assessing circulating tumor DNA (ctDNA) offers many advantages over tumor biopsy. …”
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Does the Harvesting Site Influence the Osteogenic Potential of Mesenchymal Stem Cells? by Van Thi Nguyen, Irene Tessaro, Antonio Marmotti, Camilla Sirtori, Giuseppe M. Peretti, Laura Mangiavini

“…These findings were confirmed by quantitative polymerase chain reaction (qPCR). In chondrogenic and adipogenic conditions, bone marrow cells possessed a predominant differential capacity compared with the others, illustrated by high collagen type II expression together with a cartilage-like lacuna structure and the presence of fat-specific markers, respectively. …”
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The Molecular Diagnosis Protocols of New Coronavirus (COVID-19); Specificity and Sensitivity an Overview by Abdullah Ahmed Hama, Othman Abdulrahman Mohammed, Fatima Mahmud Ali, Osama Hamid Shareef, Sardar Muhammad Wli, Sabiha Sharif, Syamand Ahmed Qadir

“…Quantitative polymerase chain reaction (qPCR) is the recommended technique used with varied sensitivity due to primers variation and specimen type. …”
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Efficacy of DNA vaccine encoding koi herpesvirus glycoprotein GP-25in common carp juvenile by immersion by Soko Nuswantoro, . Alimuddin, Munti Yuhana, Ayi Santika, Sri Nuryati, Zakki Zainun, Mira Mawardi

“…Reversetranscription polymerase chain reaction analysis showed that DNA vaccine was successfully expressed in the vaccinated fish. …”
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The ratio of COL2A1:COL1A1 in dartos tissue patients with hypospadias by Prahara Yuri, Hotman Christinus, Zico Yusuf Alfarizi, Medina Ndoye

“…The gene expression levels of COL1A1 and COL2A1 in the dartos tissue were analyzed using reverse-transcriptase polymerase chain reaction (qPCR). Results Based on the type of penile abnormality, the expression levels of COL1A1 and COL2A1 measured by qPCR were downregulated in hypospadias, with value of 0.83 (0.38–2.53) and 0.43 (0.10–5.66), respectively, compared to phimosis, which had levels of 1.85 (1.24–4.61) and 0.94 (0.26–2.47) (p < 0.001). …”
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Naturally Acquired Antibody Responses to a Synthetic Malaria Antigen AS202.11 by Rebeka Nazareth, Pius Horumpende, Tolbert Sonda, Arnold Ndaro, Edson Mollel, Eliakim Paul, Emmanuel Athanase, Jaffu Chilongola

“…Detection of P. falciparum infection was done by microscopy, malaria rapid diagnostic test, and polymerase chain reaction. ELISA was used to detect IgG antibody. …”
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