From Plasmacytoma to Rib Tuberculosis: The Case of A Breast Mass With An Unexpected Diagnosis by Hafedh Abbassi, Badra Bannour, Sarra Yacoub, Ekram Guerbej, Imen Bannour, Samir Hidar, Moncef Mokni

“…The mass was surgically excised, and histopathological analysis revealed granulomatous inflammation with caseous necrosis, suggesting TB. TB-polymerase chain reaction confirmed the diagnosis, despite negative Ziehl-Neelsen staining. …”
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Enhancing Venous Thromboembolism Risk Prediction through Novel Biomarkers and Inflammatory Indicators by Zainab Wahab Al-Maaroof

“…Plasma was collected for biomarker analysis in the following: D-dimer, thrombin generation, factor VIII levels, and inflammatory markers using enzyme-linked immunosorbent assay (ELISA) (CRP/IL-6/miRNAs), CAT, and real-time polymerase chain reaction. RESULTS: The current study revealed that D-dimer levels were much higher in VTE patients compared with controls while Thrombin generation time decreased in VTE patients, miRNAs miR-145 and miR-223 are highly expressed in those patients. …”
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Therapeutic implications and comprehensive insights into cellular senescence and aging in the tumor microenvironment of sarcoma by Pengfei Zan, Yi Zhang, Yidong Zhu, Qingjing Chen, Zhengwei Duan, Yonghao Guan, Kaiyuan Liu, Anquan Shang, Zihua Li

“…Validation cohorts, immunohistochemistry, and quantitative reverse transcription polymerase chain reaction confirmed that the predictor derived from these three genes possessed prognostic and pathological relevance. …”
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Do Salivary <i>Cullin7</i> Gene Expression and Protein Levels Provide Advantages over Plasma Levels in Diagnosing Breast Cancer? by Ceren Tilgen Yasasever, Derya Duranyıldız, Süleyman Bademler, Hilal Oğuz Soydinç

“…Gene expression levels between the two groups were analyzed by the qPCR (quantitative Polymerase Chain Reaction) method. In our study, <i>Cul7</i> mRNA and protein expression levels were examined in 60 breast cancer patients and 20 healthy female controls, and a statistically insignificant difference was found between the patient and control groups in both plasma and saliva samples (<i>p</i> > 0.05). …”
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Prevalence of Trypanosoma congolense and Trypanosoma vivax in Lira District, Uganda by Aziz, Katabazi, Adamu, Almustapha Aliero, Sarah, Gift Witto, Martin, Odoki, Simon Peter, Musinguzi

“…This study was designed to compare the prevalence of animal African trypanosomes in cattle in Lira District using microscopy and polymerase chain reaction amplification (PCR) methods. In this cross-sectional study, a total of 254 cattle from the three villages of Acanakwo A, Barropok, and Acungkena in Lira District, Uganda, were selected by simple random sampling technique and screened for trypanosomiasis using microscopy and PCR methods. …”
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Increased risk of Pneumocystis jirovecii colonization in rheumatoid arthritis patients on biologics and Janus kinase inhibitor by Ya-Chun Huang, Nan-Yao Lee, Meng-Yu Weng

“…A real-time quantitative polymerase chain reaction assay focusing on the mitochondrial large subunit ribosomal ribonucleic acid gene of PJ was applied to detect colonization. …”
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COVID-19 Pandemic and Its Impact on Perinatal Outcomes between Symptomatic and Asymptomatic Women by Inas Babic, Faten Alsomali, Sana Aljuhani, Sahar Baeissa, Inam Alhabib, Ebtisam AlAhmari, Magdy Omer, Khalid Alkhalifa

“…All pregnant women with positive reverse transcriptase-polymerase chain reaction (RT-PCR) test results for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) were enrolled, and their perinatal outcomes were analyzed in two groups based on whether they were symptomatic or not. …”
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Mapping the global landscape of chikungunya rapid diagnostic tests: A scoping review. by José Moreira, Patrícia Brasil, Sabine Dittrich, André M Siqueira

“…Diagnostics strategies rely heavily on reverse transcriptase-polymerase chain reaction (RT-PCR) and antibody detection by enzyme-linked Immunosorbent assay (ELISA). …”
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Alterations in Mucin-Associated Gene Expression on the Ocular Surface in Active and Stable Stages of Atopic and Vernal Keratoconjunctivitis by Mariko Horinaka, Jun Shoji, Akiko Tomioka, Yukiko Tonozuka, Noriko Inada, Satoru Yamagami

“…Impression cytology was performed in all patients using filter paper, and the expression levels of SPDEF, MUC1, MUC4, MUC5AC, MUC16, and eotaxin-2 mRNA were determined by real-time reverse-transcription polymerase chain reaction. Results. The results showed that the expression levels of SPDEF and MUC5AC mRNA in the active group were significantly decreased compared with those in the stable group. …”
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Keratinocyte differentiation factor 1 enhances cervical cancer cell viability and migration by activating the PI3K/AKT pathway by Chao Chen, Junhua Liao, Xingxing Sun

“…Reverse transcription polymerase chain reaction was used to detect the mRNA expression of KDF1 in cervical cancer tissues and cells. …”
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Age and diet affect self-resilience of intestinal microbiome in mice by Chenyi Shao, Shenmin Chen, Huan Yang, Mufan Li, Yinhui Liu, Shu Wen, Jing Xiao, Li Tang

“…In addition, 16S rRNA sequencing and polymerase chain reaction-denaturing gradient gel electrophoresis were performed to identify the bacterial taxa and microbiome structure, respectively.ResultsThe diversity of the gut microbiota in infant mice was recovered by the sixth week, while relative abundance of Ruminococcaceae_UCG_014 was low and did not return to normal levels. …”
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Evaluation of Anti-Cancer, Pro-Apoptotic, and Anti-Metastatic Effects of Synthetic Analogue Compound 2-Imino-7-Methoxy-4-(4-fluorophenyl)-2H-1,3-Thiazino [3,2-a] Benzimidazole on P... by Mpho Ndou, Marthe C. D. Fotsing, Michael H. K. Kengne, Edwin M. Mmutlane, Derek T. Ndinteh, Mthokozisi B.C. Simelane, Lesetja Motadi, Mpho Choene

“…Gene expression was analyzed using conventional polymerase chain reaction (PCR). Cell migration was assessed using wound healing. …”
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circRNA Expression Profile in Dental Pulp Stem Cells during Odontogenic Differentiation by Ming Chen, Yeqing Yang, Junkai Zeng, Zilong Deng, Buling Wu

“…Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) was then performed to validate the microarray results. …”
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Clinicopathologic Significance of CXCL12 and CXCR4 Expressions in Patients with Colorectal Cancer by Naomi Yoshuantari, Didik Setyo Heriyanto, Susanna Hilda Hutajulu, Johan Kurnianda, Ahmad Ghozali

“…The quantitative expressions of CXCL12 and CXCR4 messenger RNA (mRNA) were evaluated in 32 patients with adenocarcinoma-type CRC. Real-time polymerase chain reaction (qRT-PCR) was performed on formalin-fixed tissues. …”
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Gene Polymorphism of Antigen B Subunit 2 and Pathogenesis of Cystic Echinococcosis in Murine Model by Hadi M. Alsakee, Hussein M. Abdulla, Reshna K. Albarzanji

“…Minor sequence polymorphisms, 1.67%, are observed in one of the isolates, whereas remarkable DNA sequence polymorphisms are noticed in three of the isolates. The polymerase chain reaction (PCR) products sequencing profiles revealed 100% polymorphisms in four of the isolates in comparison with the source gene (AY569356.1), instead, those isolates reveal various degrees of analogy, 80.33%, 80.87–89.05%, and 89.36% to G1, G3, and G6, respectively. …”
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The role of SASP in ischemic stroke: a deep dive into cellular mechanisms by Dong Xie, Yang Liu, Fang-Biao Xu, Jin-Sheng Zhang

“…Finally, authenticated core gene expression in serum of MCAO and Sham rats using real-time fluorescent polymerase chain reaction (RT-qPCR).ResultsFourteen hub genes were discerned. …”
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CDC25B Is a Prognostic Biomarker Associated With Immune Infiltration and Drug Sensitivity in Hepatocellular Carcinoma by Zixiang Huang, Liangzhi Xu, Zhengqiang Wu, Xiaofeng Xiong, Linfei Luo, Zhili Wen

“…CDC25B expression was verified in clinical HCC tissue samples using fluorescence quantitative polymerase chain reaction (q-PCR) and protein immunoblotting (Western blot). …”
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Human Neutrophil Antigen Genotype and Allele Frequencies in Iranian Blood Donors by Behnaz Esmaeili, Behnaz Bayat, Atefe Alirezaee, Mona Delkhah, Mohammad Reza Mehdizadeh, Zahra Pourpak

“…HNA-1, HNA-3, HNA-4, and HNA-5 were genotyped, using the polymerase chain reaction sequence-specific primer (PCR-SSP) technique. …”
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AMPD1 and MTHFR genes are not associated with calcium levels in rheumatoid arthritis patients with methotrexate therapy in Indonesia by Dika P. Destiani, Sumartini Dewi, Syed A. S. Sulaiman, Sofa D. Alfian, Melisa I. Barliana, Rizky Abdulah

“…DNA was isolated and then amplified using Polymerase Chain Reaction (PCR), and genotyping was performed with Sanger sequencing. …”
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Multidrug-resistance and extended-spectrum beta-lactamase-producing lactose-fermenting enterobacteriaceae in the human-dairy interface in northwest Ethiopia. by Achenef Melaku Beyene, Mucheye Gizachew, Ahmed E Yousef, Hana Haileyesus, Ahmed G Abdelhamid, Adugna Berju, Meseret Molu Tebeje, Tigest Feleke, Baye Gelaw

“…The isolates were further characterized genotypically using multiplex polymerase chain reaction targeting the three ESBL-encoding- genes namely blaTEM, blaSHV, and blaCTX-M.…”
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