The Effect of IL-35 on the Expression of Nasal Epithelial-Derived Proinflammatory Cytokines by Mingrong Nie, Qingxiang Zeng, Luo Xi, Yiquan Tang, Renzhong Luo, Wenlong Liu

“…The expression of IL-33, IL-25, TSLP, eotaxin-1, eotaxin-2, and eotaxin-3 from supernatant was measured using real-time reverse transcription-polymerase chain reaction (RT-PCR) and enzyme-linked immunosorbent assay (ELISA). …”
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Molecular Characterization of ESBL-Producing Enterobacteriaceae in Northern Portugal by Rúben Fernandes, Paula Amador, Carla Oliveira, Cristina Prudêncio

“…In the present study we recovered some members of Enterobacteriaceae family, producing ESBL enzymes, including Escherichia coli (67.9%), Klebsiella pneumoniae (30.6%), Klebsiella oxytoca (0.5%), Enterobacter aerogenes (0.5%), and Citrobacter freundii (0.5%). β-lactamases genes blaTEM, blaSHV, and blaCTX-M were screened by polymerase chain reaction (PCR) and sequencing approaches. …”
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Occurrence, Antimicrobial Resistance, and Virulence Profiles of Salmonella Serovars Isolated from Wild Reptiles in South Africa by Lungile N. Mlangeni, Tsepo Ramatla, Kgaugelo E. Lekota, Cormac Price, Oriel Thekisoe, Che Weldon

“…The isolates were identified using polymerase chain reaction (PCR) by amplifying the invA gene and were further confirmed by 16S rRNA gene sequencing. …”
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Using spectrometric analysis for indirect estimation of 146S component concentration while measuring FMDV RNA amount by M. I. Doronin, D. V. Mikhalishin, N. Ye. Kamalova, A. V. Borisov

“…The actual results obtained in real-time reverse transcription – polymerase chain reaction (rtRT-PCR) were 97.0–99.9% consistent with the expected results of the spectrometric analysis used to determine cultural FMDV 146S component concentration. …”
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Optimizing Masks and Random Screening Test Usage within K-12 Schools by Yiwei Zhang, Maria E. Mayorga, Julie S. Ivy, Julie L. Swann

“…We also compared results when polymerase chain reaction (PCR) versus rapid antigen tests were used. …”
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Renin-angiotensin-aldosterone system in ISIAH rats with stressinduced arterial hypertension by A. D. Dubinina, E. V. Antonov, L. A. Fedoseeva, E. N. Pivovarova, A. L. Markel, L. N. Ivanova

“…We estimated the serum levels of renin, the angiotensin-converting enzyme, angiotensin II and aldosterone by an enzymelinked immunosorbent assay, and mRNA expression of RAS genes in kidney, adrenals and brain tissues was measured by the real-time polymerase chain reaction. The mRNA expression of the renin gene (Ren) in the ISIAH rats was significantly decreased as compared to the normotensive WAG rats, but plasma renin concentrations had no difference. …”
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Molecular Epidemiology of Carbapenem-Resistant Klebsiella pneumoniae in a Tertiary Hospital in Northern China by Shuqing Wang, Huanhuan Dong, Meiqi Wang, Wenbo Ma, Yue Cheng, Junliang Zhou, Yongming Cheng, Hui Xu, Xiaochen Yu

“…Antimicrobial susceptibility was determined by the VITEK2 compact system and microbroth dilution method. Polymerase chain reaction (PCR) and sequencing were used to analyze multilocus sequence typing (MLST), drug resistance determinants, and plasmid types. …”
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Establishment of Tissue Culture System of Actinidia deliciosa Cultivar “Guichang” by Weimin Zhong, Junliang Zhou, Dongmei Tang, Yaxin Huang, Futao Liu, Min Zhang, Guoli Wang, Sufang Wu, Yue He, Jingwen Tang

“…In order to breed virus-free plantlets of the kiwifruit cultivar “Guichang,” which belongs to Actinidia deliciosa, in this study, stem segments with buds were used as explants, the establishment of a tissue culture rapid propagation system was carried out, and then the virus status of tissue culture plantlets was detected via the real-time reverse transcription-polymerase chain reaction (RT-qPCR) method. The tissue culture rapid propagation system proved that the contamination and browning rates could be controlled below 20% and the survival rate could be exceeded by 70% when the single bud stem segment of “Guichang” kiwifruit was sterilized with 70% alcohol for 30–60 s and 15% NaClO for 15 min, respectively. …”
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Erythropoietin Activates Autophagy to Regulate Apoptosis and Angiogenesis of Periodontal Ligament Stem Cells via the Akt/ERK1/2/BAD Signaling Pathway under Inflammatory Microenviro... by Denghao Huang, Jie Lei, Xingrui Li, Zhonghao Jiang, Maoxuan Luo, Yao Xiao

“…Quantitative real-time polymerase chain reaction was conducted to examine mRNA expression level. …”
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Genetic epidemiology of Plasmid Mediated Class C Beta-Lactamase among Enterobacteriaceae isolates by Chuong Van Le, Nguyen Nhat Tran, Phuong Thi Be Nguyen, Nga Thi Le, Duong Thuy Le

“…Methods: Multiplex Polymerase Chain Reaction (PCR) for pAmpC identification was utilized to investigate prevalence and diversification of pAmpC among 294 cefoxitin and third-generation cephalosporin resistant Enterobacteriaceae isolated from Thailand (n= 197) and Vietnam (n= 97). …”
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Acute hepatitis of non-specified etiology by Ye. V. Tsyganova, O. O. Znoyko, N. D. Yushchuk, K. R. Dudina, M. G. Isagulyants, N. V. Petrakova, T. V. Petrova, M. I. Mikhaylov

“…To estimate pathogenic and diagnostic value of detection of hepatotropic viruses components (DNA, RNA, antigens) and antiviral immune response at acute hepatitis of non-specified etiology by the methods of enzyme-linked immunoassay (EIA), polymerase chain reaction (PCR) and Т-cellular lymphocytes proliferation in response to HCV antigens stimulation.Methods. …”
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Evolution and importance of Molecular Microbiology in the Laboratory of the Provincial Center for Hygiene, Epidemiology and Microbiology by María de Lourdes Sánchez Alvarez, Rafael Abreu Duarte, Edenis García Alvarez, Adrian Fernández García, Ermis Alexander González Martínez, Maidelys Mendoza Acosta

“…Methods: cross-sectional descriptive study carried out at the Villa Clara Molecular Biology Laboratory in the period from June 2018 to December 2023 based on the total samples processed by polymerase chain reaction, several technological platforms and various kits were used. commercial. …”
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Optimizing Laser Capture Microdissection Protocol for Isolating Zone-Specific Cell Populations from Mandibular Condylar Cartilage by Aisha M. Basudan, Yanqi Yang

“…Then, to evaluate LCM-RNA integrity, 3′/m ratios of glyceraldehyde 3-phosphate dehydrogenase (GAPDH) and beta-actin (β-Actin) using quantitative reverse transcription-polymerase chain reaction (qRT-PCR) were calculated. Both fixed and unfixed tissue sections allowed reliable identification of MCC zones. …”
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Response of Soil Bacteria to Short-Term Nitrogen Addition in Nutrient-Poor Areas by Hongbin Yin, Mingyi Xu, Qingyang Huang, Lihong Xie, Fan Yang, Chao Zhang, Gang Sha, Hongjie Cao

“…In this study, we investigated the effects of short-term exogenous N additions on soil bacterial communities using quantitative polymerase chain reaction (PCR) and Illumina Miseq sequencing in an in situ N addition field experiment. …”
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Metastatic Urothelial Carcinoma from Transplanted Kidney with Complete Response to an Immune Checkpoint Inhibitor by Ryan S. Chiang, Ashton A. Connor, Brant A. Inman, Wen-Chi Foo, David N. Howell, John F. Madden, Matthew J. Ellis, Aparna S. Rege, Michael R. Harrison

“…Molecular diagnostic studies using polymerase chain reaction amplification of short tandem repeat alleles and HLA tissue typing proved that the urothelial carcinoma originated from donor tissue. …”
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Occurrence and Antimicrobial Resistance Pattern of Listeria monocytogenes Isolated From Bovine’s Milk and Meat in Mekelle City, Ethiopia by Tesfay Hailu, Getachew Gugsa, Yisehak Tsegaye, Meselu Ahmed, Nesibu Awol

“…Isolation and identification of L. monocytogenes were done according to standard and recommended bacteriological procedures. Polymerase chain reaction (PCR) amplification of Iap, Imo0737, ORF2819, and ORF2110 genes was performed. …”
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Fermented Lentinus edodes extract containing α-glucan ameliorates concanavalin A-induced autoimmune hepatitis in mice by Xin Hu, Dan Cheng, Yingxia Zhang, Po Li, Xiaoping Wu, Junsheng Fu

“…Quantitative polymerase chain reaction, immunohistochemistry, and Western blotting showed that FLA pre-treatment inhibited the ConA-induced apoptosis of hepatocytes by down-regulating the expression of BAX and up-regulating the expression of BCL-2. …”
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Detection of mutations in plasma cell-free DNA of colorectal cancer patients and comparison with cancer panel data for tissue samples of the same cancers by Suji Min, Sun Shin, Yeun-Jun Chung

“…In this study, we aimed to verify whether the mutations identified in primary tumor tissue samples could be consistently detected in plasma cell–free DNA (cfDNA) by digital polymerase chain reaction (dPCR). We first examined the genetic alteration profiles of three colorectal cancer (CRC) tissue samples by targeted next-generation sequencing (NGS) and identified 11 non-silent amino acid changes across six cancer-related genes (APC, KRAS, TP53, TERT, ARIDIA, and BRCA1). …”
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Evaluation of Inflammation-Related Proteins in Multiple Sclerosis Disease with Relapses and Remissions by Nisa Hocaoğlu, Nur Damla Korkmaz, Birsen Elibol, Zehra Cemre Karakayalı, Şule Terzioğlu-Uşak, Azize Esra Gürsoy

“…After RNA isolation and cDNA conversion, NLRP3, ASC, NLRX1, IL-1β, and IL-18 gene expressions were measured in real time-polymerase chain reaction. In addition, the protein levels of the NLRP3 and ASC were determined from serum samples by enzyme-linked immunosorbent assay. …”
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NAT10 promotes ovarian cancer cell migration, invasion, and stemness via N4-acetylcytidine modification of CAPRIN1 by Yang Song, Min Cheng

“…The expression of NAT10 and CAPRIN1 in OC cells lines were measured using quantitative real-time polymerase chain reaction and immunoblotting. Biological behaviors of OC cells were evaluated using EdU, Transwell, sphere formation, and immunoblotting assays. …”
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