The molecular prevalence of Theileria equi in the Arabian stallion in Egypt with special reference to biochemical parameters by Noora F. Thabet, Omaima M. Kandil, Omnia M. Kandil, Khalid M. Fararh

“…The study's main goal was to determine the prevalence of T. equi in Arabian stallions in Egypt by comparing microscopic examination (ME) and conventional polymerase chain reaction (cPCR) and compare them. The study aimed to determine the liver and kidney functions of infected Arabian stallions with T. equi. …”
Get full text

Clinical Profile and Risk Factors of Group B Streptococcal Colonization in Mothers from the Eastern District of China by Jin Jiao, Weiwei Wu, Feng Shen, Zhongyuan Liu, Huiru Zhou, Gang Fan, Yuxia Zhou

“…Capsular genotypes were analyzed using polymerase chain reaction and the associated factors were evaluated via logistic regression. …”
Get full text

Fecal Microbiota and Diet of Children with Chronic Constipation by Joyce Gomes de Moraes, Maria Eugênia Farias de Almeida Motta, Monique Ferraz de Sá Beltrão, Taciana Lima Salviano, Giselia Alves Pontes da Silva

“…The fecal microbiota of the stool samples was analyzed by real-time polymerase chain reaction (PCR) using the fluorophore SYBR® Green. …”
Get full text

Type-Specific Persistence/Clearance Results in Human Papillomavirus Infections in Turkish Women by Haci Ozturk Sahin, Beril Gurlek, Bulent Demir, Fatma Silan

“…STUDY DESIGN: Data of 115 patients whose human papillomavirus and genotype detection was made with multiplex Polymerase chain reaction, and capillary electrophoresis were categorized as low-risk human papillomavirus/high risk human papillomavirus and single/multiple human papillomavirus infections, and clearance/persistence data of two years were investigated. …”
Get full text

AI allows pre-screening of FGFR3 mutational status using routine histology slides of muscle-invasive bladder cancer by Pierre-Antoine Bannier, Charlie Saillard, Philipp Mann, Maxime Touzot, Charles Maussion, Christian Matek, Niklas Klümper, Johannes Breyer, Ralph Wirtz, Danijel Sikic, Bernd Schmitz-Dräger, Bernd Wullich, Arndt Hartmann, Sebastian Försch, Markus Eckstein

“…However, current testing assays (polymerase chain reaction or next-generation sequencing) necessitate high tissue quality, have long turnover time, and are expensive. …”
Get full text

Silica-exposed patients with silicosis show shorter telomeres than do unexposed individuals: a pilot study in a population in southeastern Brazil by Marcos César Santos de Castro, Lucas de Carvalho Costa, Kaio Cezar Rodrigues Salum, Hermano Albuquerque de Castro, Patrícia Canto Ribeiro, Walter Costa, Angela Santos Ferreira Nani, Fabiana Barzotto Kohlrausch

“…We extracted DNA from buccal cells and assessed TL by multiplex quantitative polymerase chain reaction. Results: The median TL was significantly shorter in the patients with silicosis than in the unexposed controls (p < 0.0001), although it did not differ between the patients with simple silicosis and those with complicated silicosis (p = 0.961). …”
Get full text

Cystic Echinococcosis/Hydatid Cyst Coinfection with HIV: A Report from Shiraz, Iran by Yosef Sharifi, Seyed Mahmoud Sadjjadi, Hamed Nikoupour Dailami, Seyed Hamed Jafari, Mohammad Hossein Anbardar, Mohammad Bagher Khosravi

“…Histopathology examination, polymerase chain reaction (PCR), and viable protoscoleces confirmed the diagnosis of echinococcosis. …”
Get full text

A Reverse Design Method for Convective PCR Chips Featuring Precise Control of Steady-State Flow Fields by Chenfei Li, Yaping Xie, Haochen Yong, Xin Zhao, Xingxing Ke, Zhigang Wu

“…Convective Polymerase Chain Reaction (cPCR), owing to its enhanced thermal cycling efficiency, holds promise for application in the next generation of mainstream commercial PCR instruments. …”
Get full text

Expression and Secretion of CXCL-8 and CXCL-10 From Mycobacterium Bovis BCG-Infected Human Epithelial Cells: Role of IL-4 by Patricia Méndez-Samperio, Elena Miranda, Abraham Vázquez

“…Gene expression of CXCL-8 and CXCL-10 was determined by the reverse transcription (RT)-polymerase chain reaction method. The levels of immunoreactive CXCL-8 and CXCL-10 were determined by enzyme-linked immunosorbent assay. …”
Get full text

A Study of a New Technique of the CT Scan View and Disease Classification Protocol Based on Level Challenges in Cases of Coronavirus Disease by Ahmed B. Salem Salamh, Abdulrauf A. Salamah, Halil Ibrahim Akyüz

“…The chest Computer Tomography (CT scan) is used in the diagnosis of coronavirus disease 2019 (COVID-19) and is an important complement to the Reverse Transcription Polymerase Chain Reaction (RT-PCR) test. The paper aims to improve the radiological diagnosis in the case of coronavirus disease COVID-19 pneumonia on forms of noninvasive approaches with conventional and high-resolution computer tomography (HRCT) scan images upon chest CT images of patients confirmed with mild to severe findings. …”
Get full text

Evaluation of common COL2A1 gene variants in Iranian patients suspected with Stickler syndrome type I by Fatemeh Abolhasani, Hossein Abdali, Mohammad Kazemi, Bijan Movahedian Attar, Fatemeh Derakhshandeh, Majid Hosseinzadeh

“…The DNA was extracted from the patient’s peripheral blood samples, and the selected exons of the COL2A1 gene were amplified by polymerase chain reaction. Subsequently, the purified amplicons were subjected to Sanger sequencing to identify common variants associated with Stickler syndrome type I. …”
Get full text

Peste Des Petits Ruminants (PPR) in Dromedary Camels and Small Ruminants in Mandera and Wajir Counties of Kenya by R. N. Omani, G. C. Gitao, J. Gachohi, P. K. Gathumbi, B. A. Bwihangane, K. Abbey, V. J. Chemweno

“…One camel in Mandera and one goat in Wajir were confirmed positive for PPR virus (PPRV) through reverse Polymerase Chain Reaction. The analysis of sequences revealed closest nucleotide identities of obtained sequences from both goat and camel to the lineage III of PPRV albeit with 60.29% of nucleotide identity. …”
Get full text

Role of MMP-2 (-1306 C/T) Polymorphism in Pituitary Adenoma by Brigita Glebauskiene, Rasa Liutkeviciene, Alvita Vilkeviciute, Loresa Kriauciuniene, Giedrimantas Bernotas, Arimantas Tamasauskas, Dalia Zaliuniene

“…The genotyping test of MMP-2 (-1306 C/T) was carried out using the real-time polymerase chain reaction method. Results. Analysis of MMP-2 (-1306 C/T) gene polymorphism has not revealed any differences in the genotype (C/C, C/T, and T/T) distribution between the PA patients and the reference group (as follows: 50%, 44%, and 6% versus 59.75%, 33.96%, and 6.29%). …”
Get full text

Decreased Expression of GPER1 Gene and Protein in Goiter by Raquel Weber, Ana Paula Santin Bertoni, Laura Walter Bessestil, Ilma Simoni Brum, Tania Weber Furlanetto

“…We investigated GPER1 gene and protein expressions in normal thyroid and goiter using reverse transcription quantitative polymerase chain reaction (RT-qPCR) and Western blot, respectively. …”
Get full text

Identification of Potential Abnormal Methylation-Modified Genes in Coronary Artery Ectasia by Xiuchun Yang, Yijun Zong, Zhentian Zhang, Yan Zhao, Xueying Gao, Jie Zhang, Qian Hou, Renyi Li, Bing Xiao

“…Finally, after taking interaction genes between DEGs and DMGs, abnormal methylation-modified genes were identified, followed by protein–protein interaction analysis and expression validation using reverse transcriptase polymerase chain reaction. Results. A total of 152 DEGs and 4318 DMGs were obtained from RNA sequencing and the GSE87016 dataset, respectively. …”
Get full text

A Picrorhiza kurroa Derivative, Picroliv, Attenuates the Development of Dextran-Sulfate-Sodium-Induced Colitis in Mice by De-Kui Zhang, Jian-Jie Yu, Yu-Min Li, Li-Na Wei, Yi Yu, Yan-Hu Feng, Xiang Wang

“…Myeloperoxidase (MPO) activity, and SOD, MDA concentrations were measured by enzyme-linked immunosorbent assay (ELISA) while the expression of cytokine mRNAs was studied by real-time-quantitative polymerase chain reaction and also ELISA. The expression of NF-κB p65 was observed by immunohistochemistry staining and western blotting. …”
Get full text

Liver Microbiome in Healthy Rats: The Hidden Inhabitants of Hepatocytes by Xiao Wei Sun, Hua Zhang, Xiao Zhang, Peng Fei Xin, Xue Gao, Hong Rui Li, Cai Yun Zhou, Wen Min Gao, Xuan Xuan Kou, Jian Gang Zhang

“…The V3–V4 region of the 16S rRNA gene was amplified by polymerase chain reaction (PCR) and sequenced using an Illumina HiSeq 2500 platform. …”
Get full text

Clinical Efficacy Evaluation of Sirolimus in Congenital Hyperinsulinism by Somayyeh Hashemian, Reza Jafarzadeh Esfehani, Siroos Karimdadi, Rahim Vakili, Daniel Zamanfar, Amirhossein Sahebkar

“…The participants were evaluated for ABCC8, KCNJ11, or HNF4α gene mutations by polymerase chain reaction (PCR) sequencing. The participants who were unresponsive to diazoxide and octreotide therapy received 0.5 mg/m2/d of sirolimus, and the dose was gradually increased until a serum concentration of 5–15 ng/ml was achieved. …”
Get full text

Impact of Interleukin-10 Promoter Region Polymorphisms on Recurrent Miscarriage: A Case–Control Approach by Neha Sudhir, Mandeep Kaur, Sukhjashanpreet Singh, Archana Beri, Tajinder Kaur, Anupam Kaur

“…Genotype analysis of IL-10 polymorphisms rs1800872 and rs1800896 was performed using the polymerase chain reaction (PCR) restriction fragment length polymorphism and amplification mutation refractory system PCR, respectively. …”
Get full text

Gastrointestinal protozoa in pet cats from Anhui province: prevalence and molecular characterization by Hao Zhang, Xing Tong, Zhonghui Ma, Tao Zhang, Feng Wu, Qiong Wu, Congshan Yang, Chunyang Han

“…IntroductionTo investigate the prevalence of Tritrichomonas foetus, Pentatrichomonas hominis, Giardia intestinalis, Cryptosporidium, Microsporidium, and Sarcocystis in domestic cats in Anhui Province, China, and their potential role as zoonotic hosts for human infection, a total of 304 fecal samples from two different sources were screened for the presence of related pathogens.MethodsUsing microscopy, along with polymerase chain reaction (PCR) or nested PCR amplification, followed by genotyping through sequence analysis.ResultsThe infection rates of T. foetus, P. hominis, G. intestinalis, Cryptosporidium, Enterocytozoon bieneusi, and Sarcocystis were 5.6%, 0%, 1.7%, 0.7%, 2.6%, and 0%, respectively. …”
Get full text