Nasopharyngeal carriage of Streptococcus pneumoniae among Brazilian children: Interplay with viral co-infection. by Kauana Pizzutti, Juliana Comerlato, Daniele Vargas de Oliveira, Amanda Robaina, Mariana Preussler Mott, Pedro Uriel Pedrotti Vieira, Tiago Fetzner, Gabriela Rosa da Cunha, Muriel Primon de Barros, Jaqueline Verardo, Neide Maria Bruscato, João Carlos Batista Santana, Roberta Rigo Dalla Corte, Emilio Hideyuki Moriguchi, Vlademir Vicente Cantarelli, Cícero Armídio Gomes Dias

“…S. pneumoniae was detected using bacterial culture, whereas respiratory viruses were identified using quantitative polymerase chain reaction. A questionnaire was used at the time of sample collection and medical records were reviewed 14 days after participant inclusion. …”
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Gene expression profiling in B-cell non-Hodgkin lymphomas by Renas N. Saleem, Ahmed Khudair Yassin

“…The integration of gene expression biomarkers uses quantitative real-time polymerase chain reaction technique to diagnose B-NHL. Specifically, we focused on three key genes MyD88, TNF, and CSF1R whose expression profiles were analyzed in B-NHL patients and controls. …”
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Dataset for comparative analysis of precision metagenomics and traditional methods in urinary tract infection diagnosticsNCBI by Rob E. Carpenter, Sadia Almas, Vaibhav K. Tamrakar, Rahul Sharma

“…This study presents a comprehensive dataset comparing three diagnostic methodologies—microbial culture, polymerase chain reaction (PCR), and precision metagenomics (precision metagenomics)—for the detection and classification of uropathogens in urine samples from patients with suspected urinary tract infections (UTIs). …”
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Factors in Vaccine Refusal by Patients Applying for COVID-19 PCR Test by Ferhat ARSLAN, Behcet AL, Gorkem Alper SOLAKOGLU, Omer Faruk GULSOY, Cagatay NUHOGLU, Sema AYTEN

“…Objective: This study was designed to determine the reasons for vaccine rejection in patients who applied for the coronavirus disease-2019 (COVID-19) polymerase chain reaction (PCR) test but did not receive the vaccine. …”
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Polydatin ameliorates early brain injury after subarachnoid hemorrhage through up-regulating SIRT1 to suppress endoplasmic reticulum stress by Yuwei Han, Guangzhi Hao, Song Han, Tingzhun Zhu, Yushu Dong, Ligang Chen, Xinyu Yang, Xiaoming Li, Hai Jin, Guobiao Liang

“…Western blot and quantitative real-time polymerase chain reaction (PCR) analyses were performed to examine protein and mRNA expressions related to ER stress. …”
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Association between the aromatase (CYP19A1) gene variant rs10046 and cardiovascular risk in postmenopausal women by Betânia Rodrigues dos Santos, Gislaine Casanova, Thais Rasia da Silva, Karen Oppermann, Poli Mara Spritzer

“…Genotyping of the rs10046 polymorphism of the CYP19A1 gene was carried out using real-time polymerase chain reaction with allelic discrimination assays. …”
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Rational Primer and Probe Construction in PCR-Based Assays for the Efficient Diagnosis of Drifting Variants of SARS-CoV-2 by Divya RSJB Rana, Nischal Pokhrel, Santosh Dulal

“…The construction of polymerase chain reaction (PCR) assays for the reliable, efficient, and specific diagnosis of the drifting variants of SARS-CoV-2 is specifically governed by the selection and construction of primers and probes. …”
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The Anti-proliferative Effect of a Novel Glutaminase Inhibitor IN-3 on Prostate Cancer Cells by Ummuhan DEMIR, Ayse Busranur CELIK

“…The expression levels of GLS isoforms were determined by real-time polymerase chain reaction after IN-3 treatment. The metastatic prostate cancer dataset was downloaded from C-Bioportal and the expressions of GLS isoforms were analyzed. …”
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Associations between an gene polymorphism and ulcerative colitis by ARMS-PCR by Mahsa Sadat Hashemi Doulabi, Reza Goleyjani Moghaddam, Ali Salehzadeh

“…This MDM2 polymorphism was investigated in DNA samples (extracted from biopsy samples) by amplification-refractory mutation system polymerase chain reaction. The mean age of patients with ulcerative colitis was 46.5 years (range, 28 to 69 years) and that of control individuals was 45.3 years (range, 26 to 71 years). …”
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Pleiotropic effects of rabeprazole at acid-related diseases by A. V. Zaborovsky, I. V. Mayev, D. N. Andreyev, L. A. Tararina

“…Genotyping of IL-1 gene (rs16944) polymorphisms, IL-6 gene (rs1800795), IL-10 gene (rs1800896) was carried out by polymerase chain reaction with allele discrimination by TaqMan-probes. …”
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Dysregulation of c-Jun (JUN) and FBJ murine osteosarcoma viral oncogene homolog B (FOSB) in obese people and their predictive values for metabolic syndrome by Chenxi Yang, Yi Chen, Guangfeng Tang, Tongtong Shen, Li Li

“…Quantitative reverse transcription polymerase chain reaction (RT-qPCR) was used to evaluate the messenger RNA levels of JUN and FOSB in the peripheral blood of healthy volunteers (lean and obese) and patients with MetS (lean and obese), along with that in the adipose tissue and peripheral blood of obese mouse model. …”
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Association between Pneumonia Development and Virulence Gene Expression in Carbapenem-Resistant Acinetobacter baumannii Isolated from Clinical Specimens by Ji Yun Bae, Ina Yun, Kang Il Jun, Chung-Jong Kim, Miae Lee, Hee Jung Choi

“…A. baumannii isolated from respiratory specimens was examined for the expression levels of ompA, uspA, hfq, hisF, feoA, and bfnL by quantitative reverse-transcription polymerase chain reaction. Among 156 patients with CRAB from respiratory specimens, 17 and 24 met the criteria for inclusion in the pneumonia and colonization groups, respectively. …”
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Cell-Free miR-27a, a Potential Diagnostic and Prognostic Biomarker for Gastric Cancer by Jong-Lyul Park, Mirang Kim, Kyu-Sang Song, Seon-Young Kim, Yong Sung Kim

“…We initially examined plasma miRNA expression levels in a screening cohort consisting of 15 patients with gastric cancer and 15 healthy controls from Korean population, using TaqMan quantitative real-time polymerase chain reaction. We observed that the expression level of miR-27a was significantly higher in patients with gastric cancer than in healthy controls, whereas the miR-21 and miR-155a expression levels were not significantly higher in the patients with gastric cancer. …”
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De novo assembly of the mitochondrial genome of Glycyrrhiza glabra and identification of two types of homologous recombination configurations caused by repeat sequences by Guowang Zhou, Meiling Qin, Xiuli Liu, Yonghui Qi, Xiaobin Ou, Min Tang

“…We corroborated this finding via polymerase chain reaction (PCR). Furthermore, we identified chloroplast-derived sequence fragments within the mitochondrial genome, offering novel insights into the evolutionary history of plant mitochondrial genomes. …”
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Metformin Exhibits Anti‐Inflammatory Effects by Regulating microRNA‐451/CXCL16 and B Cell Leukemia/Lymphoma 2 in Patients With Osteoarthritis by Nahid Alimoradi, Amin Ramezani, Mohammad Tahami, Negar Firouzabadi

“…In addition to evaluating the clinical response using the Knee Injury and Osteoarthritis Outcome Score questionnaire, miR‐451 and miR‐15b expression levels were detected using real‐time polymerase chain reaction. The serum levels of CXCL16 and BCL‐2 were evaluated using enzyme‐linked immunosorbent assay kits before (time zero) and after treatment (month four). …”
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Isolation, genotyping and antibiotic resistance analysis in Salmonella species isolated from turkey meat in Isfahan, Iran by Farhad Lajmorak, Seyedeh Ommolbanin Ghasemian, Majid Gholami-Ahangaran

“…The conventional microbiological and biochemical tests were applied for isolation and typing of Salmonella spp. The polymerase chain reaction (PCR) was utilized for detection and typing of Salmonella strains. …”
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Integrated Approach for Biomarker Discovery and Mechanistic Insights into the Co-Pathogenesis of Type 2 Diabetes Mellitus and Non-Hodgkin Lymphoma by Zhu Y, Liu J, Wang B

“…Finally, quantitative real-time polymerase chain reaction was performed to validate hub genes in clinical samples.Results: Intersection analysis of DEGs from the T2DM and NHL datasets identified 81 shared genes. …”
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Characterization of patients admitted with pneumonia and a negative diagnostic test for COVID-19 in the intensive care unit by Rodolfo Javier Rivero Morey, Lietter Aguilar Jure, Yenisel Chang Cuesta, Inte Santana Carballosa, Victor René Navarro Machado, Lya del Rosario Magariño Abreus

“…<br /><strong>Methods</strong>: descriptive study, carried out between March/2020 and March/2021, in a series of 338 patients admitted to the Intensive Care Unit with a history of negative polymerase chain reaction to COVID-19, but with a diagnosis of acquired pneumonia in the community. …”
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Identification of the Role of Mume fructus in Treating Nocturnal Asthma Based on Network Pharmacology and In vitro Investigation by Ling-Ling Tang, Yan-Qiu Xu, Ying Yang, Xian-Hong Sun, Bo-Han Wang, Xiao-Lu Shi, Li Liu

“…Data provided included analyses of cell viability detected by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide, cell proliferation and apoptosis detected by 5-ethynyl-2’-deoxyuridine and Hoechst 33342, messenger ribonucleic acid (mRNA) levels obtained by real-time polymerase chain reaction, and protein expression detected by Western blotting. …”
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Therapeutic Potential of Liraglutide for Diabetes–Periodontitis Comorbidity: Killing Two Birds with One Stone by Man Yang, Yunqing Pang, Minyu Pei, Yuanyuan Li, Xuemin Yuan, Rongbing Tang, Jing Wang

“…The levels of Runx2 mRNA and ALP mRNA in the gingival epithelium were examined by quantitative real-time polymerase chain reaction. Results. LIRA decreased alveolar bone resorption, improved the microstructure of alveolar bone, and reduced periodontal inflammation and damage (P<0.05). …”
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