Long non-coding RNA TINCR suppresses growth and epithelial-mesenchymal transition by inhibiting Wnt/β-catenin signaling pathway in human pancreatic cancer PANC-1 cells: Insights fr... by Wei Yuan, Zhu Ping

“…Quantitative real-time polymerase chain reaction (qRT-PCR) was used to investigate TINCR expression in pancreatic cancer cells. …”
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BioFire FilmArray Blood Culture Identification (BCID) Panel and Clinical Usefulness: A Systematic Review by Niti Gor, Devesh Joshi

“…The BioFire blood culture identification (BCID) panel, a multiplex polymerase chain reaction (PCR) technique, enables earlier species identification, potentially influencing clinical decision-making. …”
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The Expression of Thymic AQP7 and Perilipin 1 (PLIN1) in Rats Fed a High-Fructose Diet is Modified by Voluntary Physical Activity by Jülide Tozkır, Nihayet Fırat, Ebru Göncü, Onur Ersoy, Pınar Tayfur, Orkide Palabıyık

“…The expression levels of AQP7 and PLIN1 were determined real-time reverse transcription polymerase chain reaction using appropriate primers. Results: The weight of the thymus tissue decreased in the FA group compared with that in the F group due to exercise (p=0.015), but the cortex and medulla structure were histologically preserved. …”
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Stachydrine targeting tumor-associated macrophages inhibit colorectal cancer liver metastasis by regulating the JAK2/STAT3 pathway by Yang Gui, Gengchen Xue, Yuyi Yuan, Jingbo Wang, Shuangjiao Deng, Fei Gao, Yushi Tian, Zhiqiang Zhao, Heng Fan

“…Subsequently, a series of assays, including quantitative reverse transcription polymerase chain reaction (qRT-PCR), flow cytometry, scratch, invasion, and tube formation assays, were conducted to confirm STA’s capacity to impede tumor cell migration, invasion, and angiogenesis in vitro. …”
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Uncovering glycolysis-driven molecular subtypes in diabetic nephropathy: a WGCNA and machine learning approach for diagnostic precision by Chenglong Fan, Guanglin Yang, Cheng Li, Jiwen Cheng, Shaohua Chen, Hua Mi

“…Finally, the expression patterns of these hub genes were validated using single-cell sequencing data and quantitative real-time polymerase chain reaction (qRT-PCR). Results Eleven GRGs showed abnormal expression in DN samples, leading to the identification of two distinct glycolysis clusters, each with its own immune profile and functional pathways. …”
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An Intriguing Case of Multisystem Inflammatory Syndrome in an Adult Patient with Remote Infection with COVID-19 and Acute Chlamydia by Madhuri Chandnani, William A. Charini, Anil Jha, Chetan Dodhia, Eduardo Haddad

“…He tested negative for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) by reverse transcriptase-polymerase chain reaction on a nasopharyngeal swab thrice within 4 days of presentation, but positive for SARS-CoV-2 immunoglobulin G antibody signifying remote infection. …”
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Encapsulation of a Pediocin PA-1 producer Pediococcus acidilactici and its impact on enhanced survival and gut microbiota modulation by M. Mokrani, A. Elie, C. Jacquot, F. Kiran, M. Urdaci

“…Furthermore, the impact of either encapsulated (E) or non-encapsulated (NE) P. acidilactici in mice gut microbiota was determined using quantitative Polymerase Chain Reaction (qPCR) and Denaturing Gradient Gel Electrophoresis (DGGE). …”
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Descriptive Investigation of Strongyloidiasis Infection and Characterization of Strongyloides stercoralis Using Morphological and Molecular-Based Methods by Nayana Gunathilaka, Nilmini Chandrasena, Tharaka Wijerathna, Yoshito Fuji, Deepa Gunasekara, Ruwan Prasanna Gunatilaka, Ranjan Premaratna

“…A multiplex polymerase chain reaction (PCR) assay was developed for the detection of S. stercoralis, Ascaris lumbricoides, and Enterobius vermicularis by designing primers specific for the ITS1 region of ribosomal DNA of S. stercoralis and A. lumbricoides and 18S region of rRNA of E. vermicularis. …”
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Expression of the Gene Encoding the Brucella abortus BAB1-0278 Antigen in Probiotic Lactococcus lactis Bacteria by donya kazemi, abbas doosti, mostafa Shakhsi niaee

“…BAB1-0278 expression was confirmed by reverse polymerase chain reaction (RT-PCR) and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and western blot techniques. …”
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Review of two decades of cholera diagnostics--how far have we really come? by Michal H Dick, Martine Guillerm, Francis Moussy, Claire-Lise Chaignat

“…Fourteen of these have also been described in the literature, with rapid chromatographic-immuno assays (CIA) featuring strongly. Polymerase chain reaction (PCR) assays maintain the ability to detect the lowest amount of bacteria; however CIAs achieve both low detection thresholds and high sensitivity and specificity, making them possible candidates for use in field conditions. …”
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PPARβ/δ Agonism with GW501516 Increases Myotube PGC-1α Content and Reduces BCAA Media Content Independent of Changes in BCAA Catabolic Enzyme Expression by Caroline N. Rivera, Jason S. Hinkle, Rachel M. Watne, Trent C. Macgowan, Andrew J. Wommack, Roger A. Vaughan

“…Metabolic gene and protein expression were assessed via quantitative real-time polymerase chain reaction (qRT-PCR) and western blot, respectively. …”
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Efficacy of vaccines based on chimeric or multiepitope antigens for protection against visceral leishmaniasis: A systematic review. by Karine Ferreira Lopes, Mariana Lourenço Freire, Silvane Maria Fonseca Murta, Edward Oliveira

“…Limiting dilution was the most used method for assessing parasite load, followed by quantitative real-time polymerase chain reaction (qPCR). Most domains had an uncertain risk of bias due to insufficient information described.…”
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ULK1 methylation promotes TGF‐β1‐induced endometrial fibrosis via the FOXP1/DNMT1 axis by Yuhua Zeng, Qing Feng

“…Expression levels of key genes were detected using western blot and quantitative ‐ real time reverse transcription polymerase chain reaction. Cell proliferation was detected by CCK‐8 and EdU staining. …”
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Preamplification Procedure for the Analysis of Ancient DNA Samples by Stefania Del Gaudio, Alessandra Cirillo, Giovanni Di Bernardo, Umberto Galderisi, Theodoros Thanassoulas, Theodoros Pitsios, Marilena Cipollaro

“…Real-time quantitative polymerase chain reaction (qPCR) was used to detect in the extracts the presence of PCR inhibitors and to estimate the DNA content. …”
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The external validity of machine learning-based prediction scores from hematological parameters of COVID-19: A study using hospital records from Brazil, Italy, and Western Europe. by Ali Safdari, Chanda Sai Keshav, Deepanshu Mody, Kshitij Verma, Utsav Kaushal, Vaadeendra Kumar Burra, Sibnath Ray, Debashree Bandyopadhyay

“…The gold standard for COVID-19 detection, quantitative-Real-Time-Polymerase-Chain-Reaction (qRT-PCR), is expensive and time-consuming. …”
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Diverse vaginal microbiota in healthy Japanese women: a combined relative and quantitative analyses by Masahiro Ito, Misaki Kataoka, Yoichi Sato, Hideki Nachi, Koji Nomoto, Koji Nomoto, Nobuhiko Okada

“…The predominant species in the growing colonies were identified using colony polymerase chain reaction, and the colonies were counted.ResultsThe vaginal microbiota was classified into four categories based on the characterization of the dominant bacterial population: Lactobacillus crispatus, Lactobacillus iners, Lactobacillus gasseri, and a diversity group. …”
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Expression of cytokeratin 19 in prostatic adenocarcinoma: a systematic review by Sina Shahshenas, Seyyed Mohammad Hosseini, Hossein Yarmohammadi, Masood Soltanipur, Mohammadreza Jalali Nadoushan

“…Eleven studies used reverse transcription polymerase chain reaction (RT-PCR) to investigate CK-19 expression, four used immunohistochemistry (IHC) staining, three used both one-step nucleic acid amplification (OSNA) and hematoxylin and eosin (H&E) methods, and three used the electrochemiluminescence (ECL) technique. …”
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EZH2 Might Affect Macrophage Chemotaxis and Anti-Inflammatory Factors by Regulating CCL2 in Dental Pulp Inflammation by Ziqi Hu, Yingyi Chen, Jie He, He Liu, Tianqian Hui

“…The expressions of anti-inflammatory cytokine interleukins (IL-4 and IL-10) and transforming growth factor-β (TGF-β) in HDPCs which were treated by EZH2 complex, CCL2 complex, and CCL2 antibody were examined by quantitative real-time polymerase chain reaction (q-PCR). Results. The expression of TNF-α gradually increased in dental pulp inflammation. …”
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Plasma mcfDNA Sequencing May Improve Usual Care Diagnostics to Detect HHV-8 Among Outpatient People with Advanced HIV by Sarah Park, Brian Epling, Morgan Richey, Daniel Lupu, Mona Mughar, Irini Sereti

“…Yet, diagnosing KS can be challenging given the diverse manifestations and current limited usual care diagnostic methods (UC; polymerase chain reaction, histopathology). Methods: Pathogen-agnostic plasma microbial cell-free DNA sequencing was applied to banked samples from 116 outpatients included in 2 previous prospective studies of patients with antiretroviral treatment-naïve, advanced HIV (CD4 count ≤100 cells/µL). …”
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Geospatial Analysis and Molecular Epidemiologic Study of Emerging Pulmonary Lophomoniasis in Iran: A National Registry-Based Study by Mohammad Amin Ghatee, Maryam Nakhaei, Ali Sharifpour, Mahdi Fakhar, Niloufar Mohamadi, Mostafa Soleymani, Siavash Abedi, Masoud Aliyali, Hossein Mehravaran

“…They were tested for BPL by a newly developed polymerase chain reaction test. The study assessed the association of Normalized difference vegetation index (NDVI), digital elevation model (DEM), and geographic latitude as environmental factors and sex and age as demographic factors on the prevalence of BPL. …”
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