Analysis of Amelogenin and Sex-determining Region on Y Chromosome Genes Obtained from Pulpal Tissue for Sex Estimation by using Multiplex Polymerase Chain Reaction by Mohammed Taha Ahmed Baban, Dena Nadhim Mohammad, Natheer Al-Rawi

“…This research aims to assess the precision of simultaneously amplifying both genes using multiplex polymerase chain reaction on samples derived from teeth that have been subjected to various forensic conditions. …”
Get full text

Melting curve analyses in the quantitative real-time polymerase chain reaction of methylated/non-methylated DNA toward the detection of oral cancer using gargle fluid by Yusaku Noma, Hayate Sawayama, Kouta Yamashiro, Keitaro Nishi, Haruka Yoshinaga, Ryota Takaoka, Tsuyoshi Sugiura, Tomonori Sasahira, Tomofumi Hamada, Yasuo Suda, Tatsuo Okui

“…We therefore sought to develop a simple screening method for the diagnosis of oral cancer by conducting a melting curve analysis in a quantitative real-time polymerase chain reaction (qPCR). The assay was performed by a qPCR of DNA from the gargle fluid of 20 patients with oral cancer and 20 healthy control subjects in the training set. …”
Get full text

Role of Chest Computed Tomography versus Real Time Reverse Transcription Polymerase Chain Reaction for Diagnosis of COVID-19: A Systematic Review and Meta-Analysis by Dina M. Ali, Lamiaa G. Zake, Nevine K. El Kady

“…The diagnosis of COVID-19 depends on detection of the viral nucleic acid by real time reverse transcription polymerase chain reaction (RT-PCR). However, false-negative RT-PCR tests are reported and could hinder the control of the pandemic. …”
Get full text

First study on molecular identification of Anaplasma ovis in sheep in southern Kazakhstan by Alexandr Ostrovskii, Madina Kadyrova, Nurdina Yerzhanova, Dinara Kamalova, Amirkhan Kassen, Nailya Tursunbay, Alexandr Shevtsov, Christian Bauer, Kassym Mukanov

Subjects: Get full text

eDNA Metabarcoding Analysis of Bony Fish Identification in Coastal Water of Gulf of Maine Using Nested Polymerase Chain Reaction of 12S rRNA Universal Primers by Bo‐Young Lee, Grant A. Milne, Corwin Freedman, Jenifer Miksis‐Olds, Bonnie L. Brown

“…The available 12S rRNA fish universal primer analysis system (MiFish and 12S‐V5) was modified using nested polymerase chain reaction (PCR) to improve targeting of fish products and reduce non‐target products. …”
Get full text

Detection of Mycobacterium tuberculosis DNA in intraocular fluid of 11 suspected tuberculous uveitis patients by multiplex PCR by Huirong Xu, Min Xu, Fang Chen, Hong Chen, Wei Du, Jing Yu

Subjects: “…Multiplex polymerase chain reaction…”
Get full text

Mutational Analysis of KCNQ1 Gene in Type 2 Diabetes Mellitus: A Case-control Study by Ankush Ganjewar, Ishwar B Bagoji, Sharan Badiger, Rudragouda B Bulagouda, Gurushnatappa Kadakol

Subjects: Get full text

Role of CFT and PCR in diagnosis of Chlamydia psittaci in experimentally infected rabbits by V. V. Evstifeev, F. M. Khusainov, S. I. Yakovlev, R. I. Shangaraev, V. I. Eremets

Subjects: Get full text

Pathogenicity role of human herpesvirus-8 in patients with acute myeloid leukemia by Zahraa Ali Abdullah, Luma Amer Yasir, Ruqaya Munther J. Ewadh, Shakir H. Mohammed Al. Alwany

Subjects: Get full text

O Serogroup-Specific Touchdown-Multiplex Polymerase Chain Reaction for Detection and Identification of Vibrio cholerae O1, O139, and Non-O1/Non-O139 by Adisak Bhumiratana, Achiraya Siriphap, Nutsarin Khamsuwan, Jednipit Borthong, Kaknokrat Chonsin, Orasa Sutheinkul

“…A novel, sensitive locus-specific touchdown-multiplex polymerase chain reaction (TMPCR), which is based on two-stage amplification pertaining to multiplex PCR and conditional touchdown strategy, was used in detecting and differentiating Vibrio cholerae serogroups. …”
Get full text

Enterocytozoon bieneusi Identification Using Real-Time Polymerase Chain Reaction and Restriction Fragment Length Polymorphism in HIV-Infected Humans from Kinshasa Province of the Democratic Republic of Congo by Roger Wumba, Menotti Jean, Longo-Mbenza Benjamin, Mandina Madone, Kintoki Fabien, Zanga Josué, Sala Jean, Kendjo Eric, Guillo-Olczyk AC, Thellier Marc

Get full text

Fetal Rhesus D Genotyping and Sex Determination from Maternal Plasma of Rhesus D-Negative Antenatal Population: The Usefulness of Conventional Polymerase Chain Reaction in Resource-limited Settings by Otchere Addai-Mensah, Edward Y. Afriyie, Samuel Asamoah Sakyi, Christian Obirikorang, Max Efui Annani-Akollor, Eddie-Williams Owiredu, Francis A. Amponsah, Richard Vikpebah Duneeh, Evans Asamoah Adu

Get full text

Effect of the host-specific toxin SnTOX3 from Stagonospora nodorum on ethylene signaling pathway regulation and redox-state in common wheat by S. V. Veselova, G. F. Burkhanova, T. V. Nuzhnaya, S. D. Rumyantsev, I. V. Maksimov

Subjects: Get full text

Hematological profile and its correlation with reverse transcription–polymerase chain reaction-based serial quantitative detection of BCR-ABL in chronic myeloid leukemia patients at a referral cancer center by Vanita Pandey, Tamojit Chaudhuri, Amitabh Upadhyay, Somshankar Chowdhury, Sujata Mitra

Get full text

Seroepidemiology and comparative diagnosis of bovine herpesvirus type-1 (bhv-1) in bulls using enzyme-linked immunosorbent assay (elisa), polymerase chain reaction (pcr) and virus isolation (vi) by Oya Bulut, Sibel Yavru

“…Two isolates from nasal swap could not be identified, while 15 out of 26 isolates from semen samples was identified as BHV-1 by EUSA Following EUSA/Ag and Polymerase Chain Reaction (PCR) method was applied for detection of BHV-1 in semen and nasal swap samples. …”
Get full text

Investigation of Trypanosoma evansi in Sumatran Elephant (Elephas maximus sumatranus) in Indonesia Using Various Methods by Kartika Purnamasari, Wisnu Nurcahyo, Muhammad Tauhid Nursalim, Lucia Tri Suwanti, Agnes Theresia Soelih Estoepangestie

Subjects: Get full text

THE REORGANIZATION OF PLANT GENOMES DURING ALLOPOLYPLOIDISATION by A. B. Shcherban

Subjects: Get full text

DETECTION OF PORCINE CIRCOVIRUS TYPE 3 IN RUSSIAN PIG HOLDINGS by M. V. Biryuchenkova, A. M. Timina, A. V. Shcherbakov

Subjects: “…polymerase chain reaction…”
Get full text

High prevalence of resistance to macrolides and fluoroquinolones in Mycoplasma genitalium isolated from patients in two Russian megalopolises – Moscow and St. Petersburg in 2021– 2... by Edelstein I.A., Gushchin A.E., Gladin D.P., Romanov A.V., Negasheva E.S., Frigo N.V., Kozlov R.S., Potekaev N.N., Kozlova N.S., Borukhovich D.G.

Subjects: Get full text

Tropheryma whipplei infection presenting as indolent endophthalmitis by Rami Waked, Jeffrey K. Moore, Brandon Winward, Sophia Ham, Howard W. Hoyt, Leyla Azis

Subjects: Get full text