Correlation between microRNA-21 expression and overweight/obesity by Shashwat Sinha, Babu Rajendran, Shomnath Vasagam, Jeyakumar Balakrishnan

“…Results: The microRNA-21 levels in circulation were analyzed using Real-Time Quantitative Reverse Transcription Polymerase Chain Reaction (qRT-PCR). The expression of microRNA-21 was statistically higher in the overweight/obese cohort compared to the normal BMI individuals (p=0.034). …”
Get full text

Complete Genome Sequence of Turnip yellows polerovirus from Iraq: Insight into the Factors Affecting the Genetic Variability of TuYV Populations by Muhannad Al-Waeli, Shirin Farzadfar, Reza Pourrrahim

“…A total of 149 symptomatic leaf samples were checked for TuYV infection using reverse transcription polymerase chain reaction (RT-PCR). Furthermore, total RNA from three symptomatic rapeseed (Brassica napus) leaf samples from Basrah-Iraq was used for RNA-Seq. …”
Get full text

Analysis of TGFBI Gene Mutations in Three Chinese Families with Corneal Dystrophy by Feng Zhao, Yuan Liu, Tao Guan

“…All 17 exons of the TGFBI gene, and the exon-intron junctions were examined by polymerase chain reaction (PCR) and direct DNA sequencing to identify and analyse gene mutations. …”
Get full text

Characterisation of IGF-1 (rs7136446) and IL-6 (rs1800795) polymorphisms among breast cancer patients in western Algeria by Fatima Zohraa Boussouf, Asmahane Medjdoub, Zineb Tahari, Moussa Messatfa, Noria Bouras, Chahinez Amira Dahmani, Sonia Amal Seddiki, Tewfik Sahraoui

“…The genotyping of selected SNPs was performed by real-time polymerase chain reaction followed by statistical analysis to assess genotypic frequencies and genetic association with BC. …”
Get full text

Rapid identification of bacterial select agents using loop-mediated isothermal amplification by Timothy E. Egbo, Candace D. Blancett, Jackie M. Payne, Christopher P. Stefan, Timothy D. Minogue, John H. Sellers, Jeffrey W. Koehler

“…Conclusion Here, we optimized assay turn-around-time, decreasing a standard 60 min traditional polymerase chain reaction (PCR) to 30 min using LAMP with positive results in as little as 5–10 min. …”
Get full text

miR-197 Participates in Lipopolysaccharide-Induced Cardiomyocyte Injury by Modulating SIRT1 by Miaomiao Liu, Ying Zhang, Xiantong Cao, Tao Shi, Yang Yan

“…We treated H9c2 cells with lipopolysaccharide (LPS), then detected the cell viability via the cell counting kit-8 (CCK-8) assay and quantified miR-197 expression via quantitative real-time polymerase chain reaction (qRT-PCR). Then, we investigated the role of miR-197 in LPS-induced H9c2 cells by CCK-8 assay, flow cytometry, lactate dehydrogenase (LDH) measurement, enzyme-linked immunosorbent assay (ELISA), qRT-PCR, and western blot. …”
Get full text

Miliary Tuberculosis Initially Presenting as an Isolated Hepatic Abscess by Chang Won Ha, Sang Deok Shin, Myung Ji Goh, Byeong Geun Song, Wonseok Kang, Dong Hyun Sinn, Geum-Youn Gwak, Yong-Han Paik, Moon Seok Choi, Joon Hyeok Lee

“…A liver biopsy was performed, revealing caseous granulomas, and the tuberculosis polymerase chain reaction result was positive. The patient was diagnosed with primary hepatic tuberculosis, which later disseminated. …”
Get full text

T Cell Repertoire and Inflammatory Bowel Disease by Kenneth Croitoru, David KH Wong, Maria E Baca-Estrada

“…Using monoclonal antibodies to T cell receptor variable-gene products or polymerase chain reaction analysis of variable-gene mRNA expression, the mucosal T cell repertoire has been examined in humans. …”
Get full text

Association between adiponectin single nucleotide polymorphisms and the risk of diabetic polyneuropathy by Noha M. Bakr, Noha A. Hashim, Nevin F. Ibrahim, Sara F. Saadawy

“…Adiponectin SNPs were genotype in 360 participants comprising diabetic sufferers with and without peripheral neuropathy and healthy volunteers via the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) approach. …”
Get full text

Differentiation of Lynch syndrome from other forms of non-polyposis colorectal cancer among Russian patients by A. S. Tsukanov, N. I. Pospehova, V. P. Shubin, I. Yu. Sachkov, S. N. Zhdankina, A. A. Ponomarenko, E. G. Rybakov, S. I. Achkasov, V. N. Kashnikov, S. A. Frolov, Yu. A. Shelygin

“…At its detection search of germline mutations by polymerase chain reaction methods, electrophoresis and direct sequencing was carried out.Results. …”
Get full text

Highly Expressing SCARA5 Promotes Proliferation and Migration of Esophageal Squamous Cell Carcinoma by Kawuli Jumai, Tangjuan Zhang, Bingzhang Qiao, Julaiti Ainiwaer, Haiping Zhang, Zhichao Hou, Idris Awut, Madinyat Niyaz, Liwei Zhang, Ilyar Sheyhidin

“…We adopted quantitative reverse-transcription polymerase chain reaction (qRT-PCR) and Celigo high-content screening (HCS) technology to screen the downstream genes of THSD7A. …”
Get full text

Expression of Glutathione Peroxidase and Glutathione Reductase and Level of Free Radical Processes under Toxic Hepatitis in Rats by Igor Y. Iskusnykh, Tatyana N. Popova, Aleksander A. Agarkov, Miguel Â. A. Pinheiro de Carvalho, Stanislav G. Rjevskiy

“…Using a combination of reverse transcription and real-time polymerase chain reaction, expression of the glutathione peroxidase and glutathione reductase genes (Gpx1 and Gsr) was analyzed by the determination of their respective mRNAs in the rat liver tissue under toxic hepatitis conditions. …”
Get full text

Bioinformation study of immune microenvironment characteristics of disulfidptosis-related subtypes in ovarian cancer and prognostic model construction by Ying Zhou, Yuhong Zhang, Yang Zhou, Yanzheng Gu, Youguo Chen, Juan Wang

“…Clinical samples were analyzed using quantitative polymerase chain reaction (qPCR) and immunohistochemistry (IHC) to validate gene expression. …”
Get full text

MicroRNA-155 Participates in the Expression of LSD1 and Proinflammatory Cytokines in Rheumatoid Synovial Cells by Ziliang Yu, Hao Liu, Jianbo Fan, Feihu Chen, Wei Liu

“…Subsequently, reverse transcription-quantitative polymerase chain reaction (RT-qPCR) was applied to probe into the level of miRNA-155 in FLS when stimulated by miRNA-155 molecules. …”
Get full text

Molecular Epidemiology of Salmonella enterica in Poultry in South Africa Using the Farm-to-Fork Approach by Melissa A. Ramtahal, Anou M. Somboro, Daniel G. Amoako, Akebe L. K. Abia, Keith Perrett, Linda A. Bester, Sabiha Y. Essack

“…Salmonella was isolated using selective media and confirmed to the genus and species level by real-time polymerase chain reaction (RT-PCR) of the invA and iroB genes, respectively. …”
Get full text

APOE Genetic Polymorphism rs7412 T/T Genotype May Be a Risk Factor for Essential Hypertension among Hakka People in Southern China by Hui Rao, Heming Wu, Zhikang Yu, Qingyan Huang

“…APOE rs429358, rs7412, and MTHFR rs1801133 were genotyped by polymerase chain reaction (PCR)-microarray. The differences in these polymorphisms between the two groups were analyzed. …”
Get full text

GJB6-D13S1830 and GJB6-D13S1854 Deletions in Patients with non-Syndromic Prelingual Deafness by Mercedes Arceo Álvarez, Estela Morales Peralta, Yuledmi Perdomo Chacón, Teresa Collazo Mesa

“…The multiplex polymerase chain reaction technique was applied, by which the genotype was described and its frequency was determined. …”
Get full text

MicroRNA-29b Suppresses Inflammation and Protects Blood-Brain Barrier Integrity in Ischemic Stroke by Xiaoqing Ma, Ho Jun Yun, Kenneth Elkin, Yunliang Guo, Yuchuan Ding, Guangwen Li

“…Methods. miR-29b from stroke patients and rats with middle cerebral artery occlusion (MCAO) were assessed using real-time polymerase chain reaction (PCR). miR-29b agomir was used to increase miR-29b expression in leukocytes via intravenous injection. …”
Get full text

NOP2/Sun RNA Methyltransferase 4 Regulates the Mammalian Target of Rapamycin Signaling Pathway to Promote Hepatocellular Carcinoma Progression by Congren Wang, Shaoying Ke, Shaoze Lin, Conglin Lin, Zhibing Cai, Lingju Hong, Qunxiong Pan

“…The expression level of NSUN4 was assessed by quantitative reverse transcription polymerase chain reaction. The western blot was utilized to determine the protein expression level of NSUN4 and mammalian target of rapamycin (mTOR) pathway-related proteins in cells and mouse tumor tissues. …”
Get full text

Gene Expression of CD70 and CD27 Is Increased in Alopecia Areata Lesions and Associated with Disease Severity and Activity by Radwa El- Sayed Mahmoud Marie, Noha M. Abd El-Fadeel, Yara El-Sayed Marei, Lina M. Atef

“…CD70 and CD27 mRNA expressions were evaluated by a quantitative real-time polymerase chain reaction in scalp biopsies from 40 AA patients (both AA lesions and non-lesional areas) and 40 healthy controls (HCs). …”
Get full text