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501
The Secretome of Bone Marrow and Wharton Jelly Derived Mesenchymal Stem Cells Induces Differentiation and Neurite Outgrowth in SH-SY5Y Cells
Published 2014-01-01“…Finally, neurite lengths assessment and quantitative real-time reverse-transcription polymerase chain reaction (RT-PCR) analysis demonstrated that CM from BMSCs and HUCPVCs differently induced neurite outgrowth and mRNA levels of neuronal markers exhibited by SH-SY5Y cells. …”
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502
Physiological value of intestinal microflora
Published 2011-09-01“…In the last years in clinical practice chemical methods, in particular gas chromatography (GC) in combination to mass spectrometry (GC-MS) are actively developed, the method of fluorescent DNA hybridization in situ (FISH) is applied, polymerase chain reaction (PCR-diagnostics) became quite widespread method of microorganisms assessment. …”
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503
Phytochemical Analysis and Antibacterial Activity of Stevia Rebaudiana Bertoni Leaves Extract against Streptococcus sanguis (A Primary Inhabitant of Dental Plaque): In Vitro Study
Published 2025-02-01“…Microscopic examination, gram stain, optochin test, catalase test and polymerase chain reaction were used to ensure the identification of S. sanguis. …”
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504
The First Record of an Aggressive Form of Ocular Tumour Enhanced by Marek’s Disease Virus Infection in Layer Flock in Al-Najaf, Iraq
Published 2024-01-01“…The diagnosis MD infection was confirmed by a Polymerase Chain Reaction (PCR) amplification of a specific region of the target gene meq of the causative agent, followed by Sanger sequencing and BLASTn search of the sequence against the NCBI nucleic acid database, resulted in Gallid alpha herpes virus 2 strain, and according to the phylogenetic analysis, the sequence from this study was uniquely clustered in its own branch in the tree. …”
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505
Association of Interleukin-18 Gene Polymorphism with Susceptibility to Visceral Leishmaniasis in Endemic Area of Bihar, an Indian Population
Published 2014-01-01“…For each locus, polymerase chain reaction (PCR) followed by restriction digestion was performed. …”
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506
Knockdown of S100A2 inhibits the aggressiveness of endometrial cancer by activating STING pathway
Published 2024-12-01“…This study was to investigate the role of S100A2 on regulating migration and invasion of endometrial cancer.Methods The mRNA and protein levels of S100A2 were obtained by quantitative real-time polymerase chain reaction, immunohistochemistry and western blot methods. …”
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507
Efficacy of CLT4A variants as immunoregulatory molecules among Vitiligo patients in Saudi Arabia
Published 2025-02-01“…Extracted genomic DNA was utilized to amplify rs231775 and rs3087243 SNPs in the CTLA4 gene, which were subsequently digested and verified using Sanger sequencing. The polymerase chain reaction and restriction fragment length polymorphism analysis laboratory data were stored in Excel and used for further statistical analysis. …”
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508
Epidemiology of Liver Cancer in Europe
Published 2000-01-01“…New laboratory techniques and biological markers such as polymerase chain reaction detection of HBV DNA and HCV RNA, as well as specific mutations related to LC, may help to provide quantitative estimates of the risk related to each these factors.…”
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509
Link-Polymorphism of 5-HTT Promoter Region Is Associated with Autoantibodies in Patients with Systemic Lupus Erythematosus
Published 2016-01-01“…To explore whether 5-HTTLPR plays a role in the pathogenesis of SLE, we tested 138 SLE patients and 138 age and sex matched health controls (HCs) for 5-HTTLPR by polymerase chain reaction (PCR) and agarose gel electrophoresis. …”
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510
Triptolide Inhibits Th17 Response by Upregulating microRNA-204-5p and Suppressing STAT3 Phosphorylation in Psoriasis
Published 2022-01-01“…Gene expression at mRNA and protein levels were examined by reverse transcription-quantitative polymerase chain reaction and Western blot analysis, respectively. …”
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511
The effect of carbonyl cyanide m-chlorophenyl-hydrazine on antibiotic susceptibility in MDR Enterobacteriaceae isolates in Babylon, Iraq
Published 2024-01-01“…Results: The results found that out of 280 clinical samples, only 134 (47.1%) isolates belonged to Enterobacteriaceae. Polymerase chain reaction (PCR) results showed that six (42.8%) out of 14 selected MDR isolates were positive for efflux pump gene oqxA. …”
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512
Genotypic Profiling of Bacillus cereus Recovered from Some Retail Foods in Ogun State, Nigeria, and Their Phylogenetic Relationship
Published 2020-01-01“…Genotypic profiling of B. cereus isolates from food was obtained by Random Amplified Polymorphic DNA-polymerase chain reaction (RAPD-PCR) using OPR13 primer. A dendrogram was drawn with the Numerical Taxonomy System of Statistic (NTSYS) software. …”
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513
Identification of the circRNA-miRNA-mRNA Regulatory Network in Bladder Cancer by Bioinformatics Analysis
Published 2021-01-01“…We detected the expression levels of these 10 mRNAs in 16 pairs of BCa tissues and adjacent normal tissues through quantitative real-time polymerase chain reaction. The differentially expressed mRNAs and central mRNAs were enriched in the processes and pathways that are associated with the growth, differentiation, proliferation, and apoptosis of tumor cells. …”
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514
Postmortem Interval Influences α-Synuclein Expression in Parkinson Disease Brain
Published 2012-01-01“…We examined expression of the full-length SNCA transcript (140 amino acid protein isoform), as well as total SNCA mRNA levels in 165 frontal cortex samples (101 PD, 64 control) using quantitative real-time polymerase chain reaction. Additionally, we evaluated the relationship of eight SNPs in both 5′ and 3′ regions of SNCA with the gene expression levels. …”
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515
Dengue virus infection among renal transplant recipients in Singapore: a 15-year, single-centre retrospective review
Published 2024-04-01“…The diagnosis of dengue was made if there were a compatible clinical syndrome and a positive dengue diagnostic assay (dengue non-structural 1 antigen, immunoglobulin M or reverse transcriptase-polymerase chain reaction). Results: Of the 31 patients diagnosed with dengue, 18 (58.1%) were deceased donor recipients. …”
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516
Evaluation of the Efficacy of Oxygen-releasing Gel as an Adjunct to Scaling and Root Planing in Chronic Periodontitis: A Split-mouth Randomized Controlled Trial
Published 2024-12-01“…A plaque sample was sent for microbiological analysis (culture for colony count and polymerase chain reaction [PCR]) at baseline and 3 months. …”
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517
Etiopathogenesis of Sheehan’s Syndrome: Roles of Coagulation Factors and TNF-Alpha
Published 2014-01-01“…PAI-1 4G/5G and TNF-α (-308 G>A) gene polymorphisms were detected with polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) methods. …”
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518
Association between TLR4 and TLR9 Gene Polymorphisms with Development of Pulmonary Tuberculosis in Zahedan, Southeastern Iran
Published 2013-01-01“…The TLR4 genes Asp299Gly, Thr399Ile, and TLR9 gene T-1486C polymorphisms were amplified by polymerase chain reaction (PCR) and then detected by PCR-restriction fragment length polymorphism (RFLP). …”
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519
POLYMORPHISM VITAMIN D RECEPTOR GENE (VDR) BSMI (RS1544410) CHRONIC PERIODONTITIS PATIENT IN JAVANESE BANYUMAS ETHNIC
Published 2021-03-01“…The samples obtained were carried out by the process of DNA isolation, Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP), and data in the Hardy-Weinberg Equilibrium (HWE) Court Lab analysis. …”
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520
A Pilot Seroprevalence Study Suggests Silent Zika virus Transmission in Bhopal Region of Central India
Published 2024-04-01“…We tested blood samples of febrile patients for ZIKV RNA using real-time polymerase chain reaction (PCR), and for the healthy individuals, we determined anti-zika immunoglobulin G (IgG) antibodies using enzyme-linked immunosorbent assay. …”
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