The Secretome of Bone Marrow and Wharton Jelly Derived Mesenchymal Stem Cells Induces Differentiation and Neurite Outgrowth in SH-SY5Y Cells by Ana O. Pires, Andreia Neves-Carvalho, Nuno Sousa, António J. Salgado

“…Finally, neurite lengths assessment and quantitative real-time reverse-transcription polymerase chain reaction (RT-PCR) analysis demonstrated that CM from BMSCs and HUCPVCs differently induced neurite outgrowth and mRNA levels of neuronal markers exhibited by SH-SY5Y cells. …”
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Physiological value of intestinal microflora by S. Yu. Kuchumova, Ye. A. Poluektova, A. A. Sheptulin, V. T. Ivashkin

“…In the last years in clinical practice chemical methods, in particular gas chromatography (GC) in combination to mass spectrometry (GC-MS) are actively developed, the method of fluorescent DNA hybridization in situ (FISH) is applied, polymerase chain reaction (PCR-diagnostics) became quite widespread method of microorganisms assessment. …”
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Phytochemical Analysis and Antibacterial Activity of Stevia Rebaudiana Bertoni Leaves Extract against Streptococcus sanguis (A Primary Inhabitant of Dental Plaque): In Vitro Study by Manar Ibrahim Ahmed, Safa Ali Hamad, Maha Abdulsalam

“…Microscopic examination, gram stain, optochin test, catalase test and polymerase chain reaction were used to ensure the identification of S. sanguis. …”
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The First Record of an Aggressive Form of Ocular Tumour Enhanced by Marek’s Disease Virus Infection in Layer Flock in Al-Najaf, Iraq by Aoula Al-Zebeeby, Ali Hadi Abbas, Haider Abas Alsaegh, Furkan Sabbar Alaraji

“…The diagnosis MD infection was confirmed by a Polymerase Chain Reaction (PCR) amplification of a specific region of the target gene meq of the causative agent, followed by Sanger sequencing and BLASTn search of the sequence against the NCBI nucleic acid database, resulted in Gallid alpha herpes virus 2 strain, and according to the phylogenetic analysis, the sequence from this study was uniquely clustered in its own branch in the tree. …”
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Association of Interleukin-18 Gene Polymorphism with Susceptibility to Visceral Leishmaniasis in Endemic Area of Bihar, an Indian Population by Dinesh Kumar, Puja Tiwary, Jaya Chakravarty, Shyam Sundar

“…For each locus, polymerase chain reaction (PCR) followed by restriction digestion was performed. …”
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Knockdown of S100A2 inhibits the aggressiveness of endometrial cancer by activating STING pathway by Chengcheng Li, Dandan Zhu, Xun Cao, Ying Li, Xiaoyuan Hao

“…This study was to investigate the role of S100A2 on regulating migration and invasion of endometrial cancer.Methods The mRNA and protein levels of S100A2 were obtained by quantitative real-time polymerase chain reaction, immunohistochemistry and western blot methods. …”
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Efficacy of CLT4A variants as immunoregulatory molecules among Vitiligo patients in Saudi Arabia by Amal F. Alshammary, Ghada BinSaif, Imran Ali Khan

“…Extracted genomic DNA was utilized to amplify rs231775 and rs3087243 SNPs in the CTLA4 gene, which were subsequently digested and verified using Sanger sequencing. The polymerase chain reaction and restriction fragment length polymorphism analysis laboratory data were stored in Excel and used for further statistical analysis. …”
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Epidemiology of Liver Cancer in Europe by F Xavier Bosch, Josepa Ribes

“…New laboratory techniques and biological markers such as polymerase chain reaction detection of HBV DNA and HCV RNA, as well as specific mutations related to LC, may help to provide quantitative estimates of the risk related to each these factors.…”
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Link-Polymorphism of 5-HTT Promoter Region Is Associated with Autoantibodies in Patients with Systemic Lupus Erythematosus by Shu Li, Shuang Liu, Fan Chen, Yuqi Cheng, Ru Bai, Aiyun Lai, Zhaoping Lu, Jian Xu

“…To explore whether 5-HTTLPR plays a role in the pathogenesis of SLE, we tested 138 SLE patients and 138 age and sex matched health controls (HCs) for 5-HTTLPR by polymerase chain reaction (PCR) and agarose gel electrophoresis. …”
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Triptolide Inhibits Th17 Response by Upregulating microRNA-204-5p and Suppressing STAT3 Phosphorylation in Psoriasis by Qi He, Xingyue Wu, Quan Shi

“…Gene expression at mRNA and protein levels were examined by reverse transcription-quantitative polymerase chain reaction and Western blot analysis, respectively. …”
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The effect of carbonyl cyanide m-chlorophenyl-hydrazine on antibiotic susceptibility in MDR Enterobacteriaceae isolates in Babylon, Iraq by Hadeel Qasim Jebur, Alaa H. Al-Charrakh

“…Results: The results found that out of 280 clinical samples, only 134 (47.1%) isolates belonged to Enterobacteriaceae. Polymerase chain reaction (PCR) results showed that six (42.8%) out of 14 selected MDR isolates were positive for efflux pump gene oqxA. …”
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Genotypic Profiling of Bacillus cereus Recovered from Some Retail Foods in Ogun State, Nigeria, and Their Phylogenetic Relationship by Titilayo O. Adesetan, Moses O. Efuntoye, Olubukola O. Babalola

“…Genotypic profiling of B. cereus isolates from food was obtained by Random Amplified Polymorphic DNA-polymerase chain reaction (RAPD-PCR) using OPR13 primer. A dendrogram was drawn with the Numerical Taxonomy System of Statistic (NTSYS) software. …”
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Identification of the circRNA-miRNA-mRNA Regulatory Network in Bladder Cancer by Bioinformatics Analysis by Jiancheng Lv, Ping-an Chang, Xin Li, Xiao Yang, Jie Han, Hao Yu, Zijian Zhou, Haiwei Yang, Pengchao Li, Jiexiu Zhang, Qiang Lu

“…We detected the expression levels of these 10 mRNAs in 16 pairs of BCa tissues and adjacent normal tissues through quantitative real-time polymerase chain reaction. The differentially expressed mRNAs and central mRNAs were enriched in the processes and pathways that are associated with the growth, differentiation, proliferation, and apoptosis of tumor cells. …”
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Postmortem Interval Influences α-Synuclein Expression in Parkinson Disease Brain by Alexandra Dumitriu, Carlee Moser, Tiffany C. Hadzi, Sally L. Williamson, Christopher D. Pacheco, Audrey E. Hendricks, Jeanne C. Latourelle, Jemma B. Wilk, Anita L. DeStefano, Richard H. Myers

“…We examined expression of the full-length SNCA transcript (140 amino acid protein isoform), as well as total SNCA mRNA levels in 165 frontal cortex samples (101 PD, 64 control) using quantitative real-time polymerase chain reaction. Additionally, we evaluated the relationship of eight SNPs in both 5′ and 3′ regions of SNCA with the gene expression levels. …”
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Dengue virus infection among renal transplant recipients in Singapore: a 15-year, single-centre retrospective review by Sophie Seine Xuan Tan, Quan Yao Ho, Sobhana Thangaraju, Thuan Tong Tan, Terence Kee, Shimin Jasmine Chung

“…The diagnosis of dengue was made if there were a compatible clinical syndrome and a positive dengue diagnostic assay (dengue non-structural 1 antigen, immunoglobulin M or reverse transcriptase-polymerase chain reaction). Results: Of the 31 patients diagnosed with dengue, 18 (58.1%) were deceased donor recipients. …”
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Evaluation of the Efficacy of Oxygen-releasing Gel as an Adjunct to Scaling and Root Planing in Chronic Periodontitis: A Split-mouth Randomized Controlled Trial by Shristi Agarwal, Shweta Bali, Priyanka Aggarwal, Amit Garg, Aruna Nautiyal, Manish Sharma

“…A plaque sample was sent for microbiological analysis (culture for colony count and polymerase chain reaction [PCR]) at baseline and 3 months. …”
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Etiopathogenesis of Sheehan’s Syndrome: Roles of Coagulation Factors and TNF-Alpha by Halit Diri, Elif Funda Sener, Fahri Bayram, Nazife Tascioglu, Yasin Simsek, Munis Dundar

“…PAI-1 4G/5G and TNF-α (-308  G>A) gene polymorphisms were detected with polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) methods. …”
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Association between TLR4 and TLR9 Gene Polymorphisms with Development of Pulmonary Tuberculosis in Zahedan, Southeastern Iran by Danial Jahantigh, Saeedeh Salimi, Roya Alavi-Naini, Abolfazl Emamdadi, Hamid Owaysee Osquee, Farzaneh Farajian Mashhadi

“…The TLR4 genes Asp299Gly, Thr399Ile, and TLR9 gene T-1486C polymorphisms were amplified by polymerase chain reaction (PCR) and then detected by PCR-restriction fragment length polymorphism (RFLP). …”
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POLYMORPHISM VITAMIN D RECEPTOR GENE (VDR) BSMI (RS1544410) CHRONIC PERIODONTITIS PATIENT IN JAVANESE BANYUMAS ETHNIC by Ryana Budi Purnama, Setiadi Warata Logamarta, Agung Dhartono

“…The samples obtained were carried out by the process of DNA isolation, Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP), and data in the Hardy-Weinberg Equilibrium (HWE) Court Lab analysis. …”
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A Pilot Seroprevalence Study Suggests Silent Zika virus Transmission in Bhopal Region of Central India by Shashwati Nema, Dipesh Kale, Romesh Jain, Ajay Halder, Manisha Shrivastava, Deepak Vaishnav, Ashvini Kumar Yadav, Divya Namdeo, Debasis Biswas

“…We tested blood samples of febrile patients for ZIKV RNA using real-time polymerase chain reaction (PCR), and for the healthy individuals, we determined anti-zika immunoglobulin G (IgG) antibodies using enzyme-linked immunosorbent assay. …”
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