Isolation and Identification of Methicillin-Resistant Staphylococcus aureus (MRSA) from Milk in Shire Dairy Farms, Tigray, Ethiopia by Weldemelak Girmay, Getachew Gugsa, Habtamu Taddele, Yisehak Tsegaye, Nesibu Awol, Meselu Ahmed, Aklilu Feleke

“…Furthermore, multiplex polymerase chain reaction (PCR) was done for the detection of mec A and fem A genes. …”
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Genetic Analysis Reveals A Missense Mutation In Reactivation Gene-2 Causing Severe Combined Immunodeficiency Disease In A Pakistani Family by Shahida Awais, Lubna Siddique, Nida Shafi, Sobia Humerah, Summyah Niazi, Wafa Omer

“…Exons specific polymerase chain reaction (PCR) was performed using oligonucleotide primers and subsequently DNA Sanger sequenced which resulted into an missense variant in the gene RAG2. …”
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Tumor Necrosis Factor-Alpha Gene Promoter Region Polymorphism and the Risk of Coronary Heart Disease by Gul Zareen Asifa, Afrose Liaquat, Iram Murtaza, Syed Ali Raza Kazmi, Qamar Javed

“…Genotyping was performed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). …”
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Molecular identification and genetic diversity analysis of Cryptosporidium spp. infecting dogs from central and northern Jordan: Detection of zoonotic genotype IId. by Rami M Mukbel, Eman M Etoom, Haifa B Hammad, Heidi L Enemark, Marwan M Abu Halaweh

“…Faecal samples were screened for Cryptosporidium using polymerase chain reaction (PCR) targeting the 18S rRNA gene, revealing an overall infection rate of 18.9% (47 out of 249). …”
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LncRNA MANCR is downregulated in non-small cell lung cancer and predicts poor survival by Yunming Tao, Jie Liu, Wenxiao Qiu, Yuanyuan Li

“…Methods After NSCLC (n = 60) and control (healthy subjects, n = 60) plasma samples, as well as NSCLC and paired non-tumor tissues from patients were collected, the levels of MANCR expression in plasma and tissues was detected using quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR). Then the correlations of MANCR expression with clinical stages were confirmed. …”
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Molecular Assessment of Blastocystis hominis in Patients with Diabetes Mellitus in Balad City, Saladin Province, Iraq by Shereen Khalid Ismaiel, Abeer Abbas Ali, Akeel Hussein Ali Al-Assi

“…Objectives: This study aims to identify the subtypes of Blastocystis sp. parasite isolated from diabetes mellitus patients using molecular techniques such as polymerase chain reaction (PCR), DNA sequencing, and phylogenetic analysis. …”
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Identification and biological characterization of the pathogen responsible for acute haemorrhagic disease in largemouth bass by Yibin Yang, Ping Deng, Hao He, Qiuhong Yang, Xiaohui Ai, Xia Zhu

“…Histopathological studies revealed that the infection caused varying degrees of necrosis and congestion in the brain and major internal organs of the largemouth bass. Polymerase chain reaction (PCR) detection showed that the LY02 isolate carried the toxin genes simA, pdi, sagA, and cfi. …”
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Association of XRCC3, XRCC4, BAX, and BCL-2 Polymorphisms with the Risk of Breast Cancer by Emre Ozoran, Fadime Didem Can Trabulus, Duygu Erhan, Bahadir Batar, Mehmet Guven

“…Gene polymorphisms were detected by using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique. 175 patients and 158 healthy controls were enrolled in the study. …”
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Fatal Multiorgan Failure Associated with Disseminated Herpes Simplex Virus-1 Infection: A Case Report by Michael Glas, Sigrun Smola, Thorsten Pfuhl, Juliane Pokorny, Rainer M. Bohle, Arno Bücker, Jörn Kamradt, Thomas Volk

“…Post-mortem polymerase chain reaction analyses confirmed a disseminated infection with HSV-1 involving various organs and tissues but excluding the central nervous system. …”
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Streptomyces sp. MUM 195J: A Promising Probiotic for Controlling Vibrio parahaemolyticus Infection in Aquaculture by Joanna Xuan Hui Goh, Loh Teng-Hern Tan, Jodi Woan-Fei Law, Giva Kuppusamy, Janaranjani M, Kooi-Yeong Khaw, Wei Hsum Yap, Kok-Gan Chan, Vengadesh Letchumanan, Learn-Han Lee, Bey-Hing Goh

“…Real-time quantitative polymerase chain reaction (RT-qPCR) analysis revealed that probiotic supplementation elevated the immune function of the animal. …”
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Shenfu Injection Protects Brain Injury in Rats with Cardiac Arrest through Nogo/NgR Pathway by Haixia Deng, Zhanhong Tang, Peng Tuo, Ruihua Wu, Si Jia, Xuan Zhao, Deqing Huang, Yuguang Gao, Zhou Lan

“…Real-time quantitative polymerase chain reaction (PCR), western blotting, and immunohistochemistry were used to detect Nogo receptor (NgR) expression in the hippocampus and cerebral cortex, and Nogo–NgR expression in CA/CPR model. …”
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Associations of Plasma BDNF and BDNF Gene Polymorphism with Cardiometabolic Parameters in Thai Children: A Pilot Study by Kanjana Suriyaprom, Banchamaphon Pheungruang, Somchai Pooudong, Pumpath Putpadungwipon, Chutima Sirikulchayanonta

“…Genotyping of BDNF G196A and C270T was performed using the polymerase chain reaction-restriction fragment length polymorphism technique. …”
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Expression evaluation of exogenous and endogenous alcohol dehydrogenase genes in transgenic Arabidopsis by Haoqiang Yu, Hong Lv, Fengzhong Lu, Qingqing Yang, Huaming Duan, Wanchen Li, Fengling Fu

“…Three homozygous T3 lines with a single integration site were screened for each of the three transformed genes by antibiotic screening, polymerase chain reaction (PCR) identification, and genomic DNA resequencing. …”
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Chorological analysis of species of the genus Dianthus in the South Caucasus region with an emphasis on the flora of Nakhchivan Autonomous Republic by Enzala Novruzova

“…The genetic analysis performed using the polymerase chain reaction method and sequencing helped to identify the level of genetic diversity and establish phylogenetic relationships between populations. …”
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Detection of methicilline resistant staphylococcus aureus carrying mecc gene in mastitic milk samples of cattle in turkey by Zafer Sayın, Aslı Sakmanoğlu, Uçkun Sait Uçan, Yasemin Pınarkara, Ali Uslu, Zeki Aras, Osman Erganiş

“…Detection of mecA and mecC gene in MRSA isolates were carried out by Polymerase Chain Reaction (PCR).<p> <b>Results:</b> Twenty eight (18.6%) out of 150 S. aureus isolates were identified phenotypically as MRSA by cefoxitin disc diffusion test. …”
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Research of the Methylation Status of miR-124a Gene Promoter among Rheumatoid Arthritis Patients by Qiao Zhou, Li Long, Guixiu Shi, Jing Zhang, Tong Wu, Bin Zhou

“…To analyze the methylation status of miR-124a loci in synovial tissues of rheumatoid arthritis (RA) patients using methylation-specific polymerase chain reaction (MSP). Materials and Methods. DNA obtained from the frozen tissue of 7 RA samples, 6 osteoarthritis (OA) samples, and 3 healthy controls were undergoing bisulfite conversion and then analyzed for miR-124a promoter methylation using MSP assay. …”
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Molecular genetic factors influencing outcomes of <i>Helicobacter pylori</i> infection at Khakasia republic inhabitants by Ye. S. Ageyeva, O. V. Shtygasheva, N. V. Ryazantseva, V. V. Tsukanov

“…Prevalence of interleukins IL1β, IL1Ra, IL8 and CYP2C19 gene polymorphism was studied by restriction analysis, VacA +– and CagA +– subtypes of H. pylori – by polymerase chain reaction.Results. Peptic ulcer was associated to S1S2 Vac A subtypes at Caucasians, while at Khakases – to Cag А strains of H. pylori. …”
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Characteristics of ocular findings of patients with coronavirus disease 2019 in China by Shudan Wang, Jiaoyang Feng, Rui Zhu, Xin Jin, Yiheng Ding, Di Jin, Yu Guo, Hong Zhang

“…Inclusion criteria for COVID-19 was confirmed testing positive via a polymerase chain reaction (PCR) test or testing positive for COVID-19 via an antigen kit. …”
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Skeletal Muscle-Specific CPT1 Deficiency Elevates Lipotoxic Intermediates but Preserves Insulin Sensitivity by Wanchun Shi, Siping Hu, Wenhua Wang, Xiaohui Zhou, Wei Qiu

“…Phosphorylated AKT (pAkt) and glucose transporter 4 (Glut4) were determined with real-time polymerase chain reaction (RT-PCR). Insulin tolerance test, glucose tolerance test, and pyruvate oxidation were performed in both groups. …”
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Bacteria Load Determination of the Intestinal Microbiota and Identification of Spiroplasma and Wolbachia in Anopheles gambiae by Haziz Sina, Kamirou Chabi-Sika, Razaki Ossè, Akim Socohou, Ibrahim A. Abibou, Hafiz Salami, Germain Gil Padonou, Adolphe Adjanonhoun, Lamine Baba-Moussa

“…Comparison of batches bacterial load was carried out with the variance analysis test (ANOVA). Finally, polymerase chain reaction (PCR) was performed to investigate the presence of a few bacterial genera influencing the vector capacity of An. gambiae. …”
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