Occurrence of Bordetella Infection in Pigs in Northern India by Sandeep Kumar, Bhoj R. Singh, Monika Bhardwaj, Vidya Singh

“…In the present study, occurrence of Bordetella infection in apparently healthy pigs was determined in 392 pigs sampled to collect 358 serum samples and 316 nasal swabs from Northern India by conventional bacterioscopy, detection of antigen with multiplex polymerase chain reaction (mPCR), and detection of antibodies with microagglutination test (MAT) and enzyme linked immune-sorbent assay (ELISA). …”
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Circulating Tumor DNA in a Breast Cancer Patient's Plasma Represents Driver Alterations in the Tumor Tissue by Jieun Lee, Sung-Min Cho, Min Sung Kim, Sug Hyung Lee, Yeun-Jun Chung, Seung-Hyun Jung

“…This mutation was subsequently validated by digital polymerase chain reaction in liquid biopsy. Our result confirm that driver alterations identified in the tumor tissue were detected in liquid biopsy by targeted NGS as well, and suggest that a higher depth of sequencing coverage is needed for detection of genomic alterations in a liquid biopsy.…”
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METTL3 Attenuates LPS-Induced Inflammatory Response in Macrophages via NF-κB Signaling Pathway by Jinghua Wang, Shushan Yan, Hongying Lu, Shufeng Wang, Donghua Xu

“…We used quantitative real-time polymerase chain reaction to detect the expression of METTL3 in RA patients and controls as well as the macrophage cell line. …”
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Varicella Zoster Virus Meningitis in a Young Immunocompetent Adult without Rash: A Misleading Clinical Presentation by Thomas Pasedag, Karin Weissenborn, Ulrich Wurster, Tina Ganzenmueller, Martin Stangel, Thomas Skripuletz

“…Further CSF analyses, including polymerase chain reaction (PCR) and detection of intrathecal synthesis of antibodies, showed a VZV infection. …”
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Comparative transcriptome analyses of different orthosiphon aristatus tissues reveal differentially expressed genes associated with flavonoid biosynthesis by Qiaoxue Wang, Huan Long, Shumeng Liu, Xixi Xu, Qin Zhang, Shanyong Yi

“…Several of these key enzyme genes were further validated by quantitative real-time polymerase chain reaction (qRT-PCR). Our research provides novel insights into flavonoids synthetic biology, and highlights O. aristatus root may serve as a valuable resource for medicinal use.…”
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Antibiotic resistance gene occurrence in poultry farms in northeast Brazil by Henrique Francisco de Almeida, Paulo Ricardo Conceição Marques Trindade, César Roberto Viana Teixeira, Claudson Oliveira Brito, Silvio Santana Dolabella, Sona Jain, Maíra Pompeu Martins, Ana Andréa Barbosa

“…The presence of ARGs in the obtained material was tested by Polymerase Chain Reaction (PCR) using primers for selected ARGs. …”
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Progressive Multifocal Leukoencephalopathy in a Multiple Sclerosis Patient Diagnosed after Switching from Natalizumab to Fingolimod by Tim Sinnecker, Jalal Othman, Marc Kühl, Imke Metz, Thoralf Niendorf, Annett Kunkel, Friedemann Paul, Jens Wuerfel, Juergen Faiss

“…We report a MS patient in whom PML was highly suspected solely based on MRI findings after switching from NTZ to fingolimod despite repeatedly negative (ultrasensitive) polymerase chain reaction (PCR) testing for JC virus DNA in cerebrospinal fluid. …”
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First molecular diagnosis of the human pathogen Rickettsia raoultii and other spotted fever group rickettsiae in Sudanese ixodid ticks from domestic ruminants by Nagwa Eisawi, Jabbar Ahmed, Mohammed A. Bakheit, Dina A. Hassan, Mohammed O. Hussien, Abdel Rahim M. El Hussein

“…This study aimed to detect and characterize spotted fever group (SFG) rickettsiae in ixodid ticks infesting domestic ruminants in Khartoum State. Methods Polymerase chain reaction targeting both genes that encode for citrate synthase (gltA) and outer membrane protein (ompA) was performed for the presence of SFG rickettsia followed by sequence and phylogenetic analysis. …”
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Role of HLA alleles polymorphism in systemic lupus erythematosus: A prospective study from North India by Ranjan S. Rana, Bitan Naik, Mahima Yadav, Usha Singh, Anup Singh, Shailja Singh

“…HLA typing was done by polymerase chain reaction (PCR)-sequence-specific oligonucleotide (SSO) method (SSO probe). …”
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Frequency of Drug Resistance Gene Amplification in Clinical Leishmania Strains by C. Mary, F. Faraut, M. Deniau, J. Dereure, K. Aoun, S. Ranque, R. Piarroux

“…Relative quantification experiments by means of real-time polymerase chain reaction showed that multidrug resistance gene amplification is the more frequent event. …”
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Genetic tracking of Crimean-Congo haemorrhagic orthonairovirus in Hyalomma population infesting cattle in Nigeria. by Oluwafemi Babatunde Daodu, Joseph Ojonugwa Shaibu, Rosemary Ajuma Audu, Daniel Oladimeji Oluwayelu

“…The CCHFV S-segment was detected using specific primers via reverse transcriptase polymerase chain reaction, followed by sequencing of amplicons. …”
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The Correlation between Oral Candida albicans and Interlukine-23 in Diabetic Patients in Hilla City, Iraq by Huda Reyad Al-Harba, Nebrass Nasir-Alla, Azher Abdulhafidh Jabir

“…The oral swabs of all samples were identified by real-time polymerase chain reaction (PCR), blood samples were obtained from all patients and control in order to determine hemoglobin A1c (HbA1c) and IL-23. …”
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CRISPR-Based Programmable Nucleic Acid-Binding Protein Technology Can Specifically Detect Fatal Tropical Disease-Causing Pathogens by Md. Rashidur Rahman, Toma Rani Majumder, Md. Aminul Islam Apu, Alok K. Paul, Afrina Afrose, Biplab Kumar Dash

“…However, standard pathogen detection methods, including culture and microscopic observation, antibody-based serologic tests, and primarily polymerase chain reaction (PCR)-oriented nucleic acid screening techniques, have shortcomings that limit their widespread use in responding to outbreaks and regular diagnosis, especially in remote resource-limited settings (RLSs). …”
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An Inflammatory Pseudotumor Arising from Pterygopalatine Fossa with Invasion to the Maxillary Sinus and Orbital Cavity by Hidenori Yokoi, Takuya Yazawa, Yuma Matsumoto, Tetsuya Ikeda, Masachika Fujiwara, Yasuo Ohkura, Naoyuki Kohno

“…To obtain a definitive diagnosis from histopathological analysis, the lesion was biopsied, and she was diagnosed as the inflammatory pseudotumor with the immunohistochemical study and multiplex polymerase chain reaction-based clonality assays. The patient had a lymphoid-predominant lesion that responded to radiotherapy but corticosteroids were not effective. …”
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Investigation of genetic structure of various cat breeds by using d-loop polimorphism in turkey by Elif Yılmaz Şahin, Vahdettin Altunok, Ercan Kurar

“…The products of Polymerase Chain Reaction (PCR) CEQ-8000 are determined by using the System of Genetic Analysis of Beckman Coulter and sequence of sequences resoluted with capillary electrophoresis are determined.…”
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A Comprehensive Study of the Relationship between the Production of β-Lactamase Enzymes and Iron/Siderophore Uptake Regulatory Genes in Clinical Isolates of Acinetobacter baumannii... by Mahyar Porbaran, Hamed Tahmasebi, MohammadReza Arabestani

“…Also, IUS genes were detected by the polymerase chain reaction (PCR) method. Results. Seventy-two (72) A. baumannii isolates were collected from a different clinical specimen. …”
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Evaluation of antifungal effect of amphotericin B in comparison with nystatin on Candida species derived from patients undergoing head-and-neck radiotherapy

“…The isolates were identified by polymerase chain reaction-restriction fragment length polymorphism. …”
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Dietary Quercetin Regulates Gut Microbiome Diversity and Abundance in <i>Apis cerana</i> (Hymenoptera Apidae) by Haodong Wu, Conghui Ji, Ruisheng Wang, Lijiao Gao, Wenhua Luo, Jialin Liu

“…Gut samples were collected on the 5th and 9th days, subjected to a polymerase chain reaction and 16S rRNA sequencing, and analyzed. …”
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B-doped nano-hydroxyapatite facilitates proliferation and differentiation of osteoblasts by Qiao Kang, Wenhao Wang, Shengtao Wu, Ganggang Hu

“…Western blotting for protein expression detection together with real-time quantitative polymerase chain reaction for mRNA expression measurement were carried out on those related to hBMSC proliferation and differentiation. …”
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Clinical Performance of Rapid Antigen Tests for the Detection of SARS-CoV-2 Infection in the Emergency Department and Community: A Retrospective Study by Pei-Chin Lin, Hung-Pin Chiu, Fang-Yi Cheng, Chih-Chun Chang, Fang-Yeh Chu

“…This study aimed to provide evidence regarding the diagnostic performance of SARS-CoV-2 rapid antigen tests compared with the real-time reverse transcription-polymerase chain reaction (RT-PCR) test in the emergency department (ED) and community. …”
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