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441
Sex Differences in the Relation between Comorbidities and Prognosis in Hospitalized Patients with COVID-19
Published 2022-01-01“…Real-time reverse-transcription polymerase chain reaction (RT-PCR) testing was done upon admission. …”
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442
Molecular Epidemiology of Enteric Adenovirus Gastroenteritis in under-Five-Year-Old Children in Iran
Published 2016-01-01“…Samples were tested for HAdV-40 and HAdV-41, using a specific pair of primers in polymerase chain reaction (PCR) method. Results. HAdV-40 and HAdV-41 were detected in 132 (5.18%) of the patients with diarrhea. …”
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443
Comparison of Different Phenotypic Tests versus PCR in the Detection of Carbapenemase-Producing Pseudomonas aeruginosa Isolates in Hamadan, Iran
Published 2021-01-01“…We evaluated the performance of MHT, CarbaNP, CDDT, and CIM tests in comparison to polymerase chain reaction (PCR) for the detection of carbapenemase-producing isolates. …”
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444
Development of a conjunctival contact–type drug delivery device for latanoprost using hyaluronic acid
Published 2025-12-01“…On day 10, we quantified gene expression of inflammatory cytokines and IOP-affecting proteins using polymerase chain reaction, and performed histological and immunohistochemical analyses. …”
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445
New Record of Different Yeast Isolates from Patients with Ear Infections Using DNA Sequencing in Al-Najaf Governorate, Iraq
Published 2024-12-01“…Amplification of genes was carried out according to the experimental protocol, and the polymerase chain reaction products of Candida spp were sent to Macrogen Lab, USA, for sequencing. …”
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446
lncRNA RUNDC3A-AS1 Regulates Proliferation and Apoptosis of Thyroid Cancer Cells via the miR-151b/SNRPB Axis
Published 2022-01-01“…Relative expression levels of RUNDC3A-AS1, microRNA (miR)-151b, and small nuclear ribonucleoprotein polypeptides B and B1 (SNRPB) were examined by reverse transcription quantitative polymerase chain reaction (RT-qPCR) in THCA cells. The localization of RUNDC3A-AS1 in THCA cells was detected by subcellular fractionation assay. …”
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447
Microscopic and molecular detection of Cytauxzoon spp. in cats in Mosul city, Iraq
Published 2025-01-01“…To achieve this goal, microscopic and conventional polymerase chain reaction technology was used, and the genetic analysis of Cytauxzoon spp. was verified. …”
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448
16S rRNA and Omp31 Gene Based Molecular Characterization of Field Strains of B. melitensis from Aborted Foetus of Goats in India
Published 2013-01-01“…All the isolates produced amplification products of 1412 and 720 bp in polymerase chain reaction with genus and species specific 16S rRNA and omp31 gene based primers, respectively. …”
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449
Comparison of and as Reporter Genes for Noninvasive Imaging of Bone Mesenchymal Stem Cells Transplanted into Infarcted Rat Myocardium
Published 2011-07-01“…The transfection efficiency of Ad/eGFP/rNIS and Ad/eGFP/hNIS to rBMSCs was measured by real-time polymerase chain reaction, flow cytometry, Western blot, and immunofluorescence staining. …”
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450
Glutathione S-transferase polymorphism in ethnic groups living in Eastern Siberia
Published 2017-11-01“…To this end, genetic typing of DNA samples was performed by polymerase chain reaction. Statistically significant differences in the frequency of occurrence of alleles A, B, C of the GSTP1 gene (р = 0.026) were found between the groups of teenagers compared. …”
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451
Epithelial and immune transcriptomic characteristics and possible regulatory mechanisms in asthma exacerbation: insights from integrated studies
Published 2025-01-01“…Asthma animal and in vitro cell line models were established to verify the key regulatory genes expression by employing quantitative reverse transcription polymerase chain reaction (qRT-PCR).ResultsScRNA-seq analysis identified 7 epithelial subpopulations and 14 distinct immune cell types based on gene expression profiles. …”
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452
New insights into the DNA extraction and PCR amplification of minute ascomycetes in the genus Laboulbenia (Pezizomycotina, Laboulbeniales)
Published 2024-06-01“…In particular, the genus Laboulbenia is notorious for low success with DNA extraction and polymerase chain reaction (PCR) amplification. This is attributed to the presence of melanin, a molecule known to inhibit PCR, in the cells. …”
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453
Comparison of the sequences of the viral capsid protein 1 and viral capsid protein 2 encoded genes in symptomatic and asymptomatic cases of canine parvovirus 2 in dogs
Published 2025-01-01“…Materials and Methods: Symptomatic and asymptomatic cases (n = 100/each) were tested by a polymerase chain reaction targeting VP1 and VP2 genes. Results: The analysis revealed numerous synonymous and nonsynonymous mutations in VP1 and VP2 and in the intergenic sequence. …”
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454
CRIP1 inhibits cutaneous melanoma progression through TFAM-mediated mitochondrial biogenesis
Published 2025-02-01“…Real-time quantitative polymerase chain reaction (RT-qPCR) and Western blots (WB) were used to detect stable overexpression and knockdown of CRIP1 in melanoma cells. …”
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455
A Patient with 38 Years of Smoking History and Severe Shortness of Breath
Published 2023-12-01“…The diagnosis was established: bilateral bronchiolitis associated with a new coronavirus infection (SARS-CoV-2 polymerase chain reaction — positive); chronic obstructive pulmonary disease stage II according to GOLD (Global Initiative for Chronic Obstructive Lung Disease), exacerbation. …”
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456
Biofilm Formation and blaOXA Genes Detection Among Acinetobacter baumannii from Clinical Isolates in a Tertiary Care Kirtipur Hospital, Nepal
Published 2021-11-01“…The antimicrobial susceptibility test was carried out by the Kirby-Bauer disc diffusion method, carbapenemase production detection was confirmed by Modified Hodge Test, and target resistant genes were detected by polymerase chain reaction. (3) Results: Out of 90 clinical specimens, 64.44% (58/90) showed bacterial growth, whereas 32.75% (19/58) isolates were identified as A. baumannii. …”
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457
Citrus transcription factor CsERF1 is involved in the response to citrus tristeza disease
Published 2025-01-01“…CsERF1 belongs to the citrus AP2/ERF transcription factor family.MethodsTo determine the role of CsERF1 on CTV resistance in citrus and the effects of the exongenous hormone application on CsERF1 in citrus, the expression of related genes was quantitatively analyzed by quantitative reverse transcription polymerase chain reaction (RT-qPCR) in this study.ResultsThe expression profile showed that the expression level of CsERF1 in roots was significantly lower under CTV infection than in healthy plants, while the expression level in stems was significantly increased. …”
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458
Clonally Related Plasmablastic Lymphoma Simultaneously Occurring with Diffuse Large B-Cell Lymphoma
Published 2020-01-01“…We extracted DNA from the two lymphomas for polymerase chain reaction and sequenced the amplified immunoglobulin heavy variable genes and the complementarity-determining region- (CDR-) 3. …”
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459
Exploring Unique Extracellular Vesicles Associated Signatures: Prognostic Insights, Immune Microenvironment Dynamics, and Therapeutic Responses in Pancreatic Adenocarcinoma
Published 2024-01-01“…Differential expression of selected lncRNAs in both cellular and exosomal fractions of PAAD was further confirmed through quantitative polymerase chain reaction (qPCR). Eight DELs were identified from TCGA and GTEx datasets, and two exosomal lncRNAs exhibited a significant correlation with overall survival, warranting further investigation. …”
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460
Blood Borne Viral Infections in Transplantation: Hepatitis Viruses and Retroviruses
Published 1993-01-01“…Despite recent improvements in anti-HCV testing, current screening assays underestimate the incidence of transmission and prevalence of HCV infection among immunosuppressed organ recipients: evidence of ongoing HCV infection depends on detection of HCV RNA by polymerase chain reaction. Determination of I-IIV-1 p24 antigen may facilitate identification of prospective organ donors in ll1e window period of early infection and may enhance serological follow-up of allograft recipients al risk of transplantation-associated HIV-1 infection. …”
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