Improvement of Helicobacter pylori eradication efficacy at stomach and duodenum peptic ulcers by Natalya O. Schanova, Lyudmila V. Prokhorova

“…All patients underwent quantitative serological test for H. pylori-specific IgG antibodies, stool test for H. pylori antigen, fibrogastroscopy with stomach mucosa (SM) biopsy and rapid urease test, microscopic examination of SM biopsy specimens, polymerase chain reaction for H. pylori DNA in SM biopsy specimens. …”
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Plasma lncRNA GAS8-AS1 as a Potential Biomarker of Papillary Thyroid Carcinoma in Chinese Patients by Dongxue Zhang, Xin Liu, Bojun Wei, Guoliang Qiao, Tao Jiang, Zhenwen Chen

“…We investigated the expression profile of plasma GAS8-AS1 in 97 patients with PTC and 39 patients with nodular goiter by quantitative real-time polymerase chain reaction. Results. GAS8-AS1 expression in plasma was downregulated in patients with PTC in comparison with those in nodular goiters (P<0.001). …”
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Optimal Molecular Methods in Detecting p190BCR-ABL Fusion Variants in Hematologic Malignancies: A Case Report and Review of the Literature by Rebecca J. Sonu, Brian A. Jonas, Denis M. Dwyre, Jeffrey P. Gregg, Hooman H. Rashidi

“…We report a case of a 76-year-old male with a new diagnosis of B-ALL who was initially found to be BCR-ABL1 negative by quantitative polymerase chain reaction (PCR). A concurrent qualitative PCR was performed which detected a positive BCR-ABL1 result that was confirmed by a next generation sequencing (NGS) based assay and identified as the rare fusion variant e1a3 of p190BCR-ABL. …”
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Geroprotection from ergothioneine treatment in Drosophila melanogaster by improving intestinal barrier and activation of intestinal autophagy by Hongyu Pan, Qianwang Zheng, Yuan Zou, Guozhao Luo, Mingzhen Tu, Na Wang, Jingru Zhong, Liqiong Guo, Junfang Lin

“…In present study, the metagenomics sequencing and quantitative real-time polymerase chain reaction analysis were employed to characterize the composition and diversity of gut microbiome in aging flies. …”
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A Case of John Cunningham Virus Induced Rhombencephalitis after Rituximab Therapy for Idiopathic Thrombocytopenic Purpura by Silpita Katragadda, Varshaa Koneru, Genevieve Devany, Aaron S. DeWitt, Vasudev H. Tati

“…Magnetic resonance imaging (MRI) showed right cerebellar lesion and cerebrospinal fluid (CSF) polymerase chain reaction (PCR) was positive for JC virus. …”
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African swine fever in the Republic of Crimea in 2015–2018 by N. G. Kosharny, S. I. Danylchenko, M. A. Pasunkina, D. V. Gadzevich, N. G. Vorotilova

“…In 2018, ASF virus was detected with polymerase chain reaction (PCR) in 8 samples of pathological materials from wild boars, that was 0.1% out of total number of the samples tested in the FGBI “ARRIAH” Branch in the Republic of Crimea and FGI RC “Regional State Veterinary Laboratory of the Republic of Crimea”. …”
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First detection of recombinant variant of African swine fever virus in the Russian Federation (brief communication) by A. S. Igolkin, R. S. Chernyshev, N. G. Zinyakov, E. O. Morozova, A. R. Shotin, K. N. Gruzdev, I. A. Chvala, A. Mazloum

“…Testing biomaterial from dead pigs in real-time polymerase chain reaction showed no changes in sensitivity or specificity, despite significant genetic distinctions between the recombinant and genotype II isolates that are enzootic to the Russian Federation. …”
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A Real-Time PCR Assay for Bat SARS-Like Coronavirus Detection and Its Application to Italian Greater Horseshoe Bat Faecal Sample Surveys by Andrea Balboni, Laura Gallina, Alessandra Palladini, Santino Prosperi, Mara Battilani

“…Numerous studies have been carried out to identify new bat viruses related to SARS-coronavirus (bat-SARS-like CoVs) using a reverse-transcribed-polymerase chain reaction assay. However, a qualitative PCR could underestimate the prevalence of infection, affecting the epidemiological evaluation of bats in viral ecology. …”
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Association of FAS and FAS Ligand Genes Polymorphism and Risk of Systemic Lupus Erythematosus by Bita Moudi, Saeedeh Salimi, Farzaneh Farajian Mashhadi, Mahnaz Sandoughi, Zahra Zakeri

“…In this case-control study, 106 SLE patients and 149 sex, age, and ethnicity matched healthy controls were genotyped for the Fas A-670G and FasLC-844T polymorphisms by polymerase chain reaction-restriction fragment length polymorphism method (PCR-RFLP). …”
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Immunosuppressants in modern approaches to treatment of inflammatory bowel diseases by O. B. Schukina, A. G. Kharitonov, A. M. Kharitidis, T. E. Ivashchenko, Yu. A. Nasykhova, T. V. Gabrusskaya, A. Yu. Baranovsky

“…From general register of IBD patients, those with bone marrow toxicity on a background of immunosuppressants in past history (n=21) were selected for assessment of TPMT gene allelic variants by polymerase chain reaction.Results. Only in one patient (4,7%) with Crohn's disease and leukopenia (3,85·109/l) and thrombocytopenia (80·109/l), developed in the remote terms (61 wks) after onset of azathioprin intake, TPMT*3A polymorphism has been revealed, as point mutations 460 G&gt; A and 719A&gt;G. …”
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Sarcoidosis and Non-small Cell Lung Cancer: Expression of miR-145, miR-301, and miR-449 by Negin Khosravi, Abdolreza Mohamadnia, Changiz Ahmadi Zadeh, Naghmeh Bahram, Saeid Ghorbian

“…This research aimed to analyze the levels of miR-449, miR-301, and miR-145 in lymph node tissue of individuals with sarcoidosis compared to a control group using the real-time polymerase chain reaction (PCR) and compare their expression with non-small cell lung cancer tissue. …”
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The genomic copy number determination of improved Cre in transgenic mice using standards derived from megaprimer PCR constructed tandem repeats by Ge Diao, Min Tian, Runbo Li, Jie Huang, Jian Han, Jianxin Guo

“…We demonstrate a real-time quantitative polymerase chain reaction (RT-qPCR) based method for genomic copy number determination of improved Cre (iCre), which was tested in a transgenic mouse model of CYP19A1-iCre. …”
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Gene expression and immunohistochemistry analysis of ADAMTS-1 and its substrates in odontogenic keratocyst by Osvaldo Rodrigues de Souza Neto, Antonia Taiane Lopes de Moraes, Hellen Thais Fuzii, Antonio Guilherme Maneschy Faria, Vanessa Morais Freitas, Maria Sueli da Silva Kataoka, Sérgio de Melo Alves, João de Jesus Viana Pinheiro

“…Methods Immunohistochemistry and polymerase chain reaction (PCR) were conducted on 30 cases of odontogenic keratocysts (OKCs) and 20 dental follicles (DFs). …”
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Coronavirus Disease-19 associated arthritis – An observational study by Debanjali Sinha, Sumantro Mondal, Alakendu Ghosh

“…Methods: We have collected the clinical data of 14 patients over the past 6 months who have developed arthralgia or arthritis during or after symptomatic COVID-19 infection, proven by a positive reverse transcription-polymerase chain reaction test from nasopharyngeal swab. Results: The most common symptoms during COVID-19 infection in the 14 patients were fever and myalgia, being present in 92.8% and 64.3% patients, respectively. …”
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Study about the Expression of CypA Proteins and Its Blocking Effect in the Cerebellum from FMR1 Gene Knockout Mice by Electroacupuncture of DU1 Acupoint by Dong LIN, Wanping BU, Xiaoting YANG

“…Objective:To observe the effect of DU1 acupoint on the expression of CypA-ERK1/2 pathway related proteins from the cerebellum of FMR1 gene knockout (KO) mice, so as to explore its mechanism.Methods:Identification of murine gene phenotype by polymerase chain reaction (PCR), and choosing a Fragile X-1(FMR1) deficiency in the KO mice with 28 days after birth, total 24 KO mice were randomly divided into blank group, DU1 acupoint group, DU1 acupoint group with blocking and non-acupoint group, with 6 mice in each group. …”
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Prevalence of Transfusion Transmitted Virus Infection in Hemodialysis Patients and Injection Drug Users Compared to Healthy Blood Donors in Isfahan, Iran by Behrooz Ataei, Alireza Emami Naeini, Farzin Khorvash, Mohammad Reza Yazdani, Abbas-Ali Javadi

“…Samples were tested for TTV DNA by means of real-time polymerase chain reaction (PCR). Results. The mean age was years. …”
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Evaluation of hearing screening results of newborns born to SARS-CoV-2 positive pregnant women by Ünal Akça, Emre Sanrı, Gülfer Akça

“…The newborns were born to mothers who tested positive for SARS-CoV-2 polymerase chain reaction (PCR) during pregnancy between March and September 2022. …”
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التشخيص الجزيئي لنبات البوثس Epipremnum aureum المستزرع في محافظة نينوى لاول مرة في العراق by شهد ابراهيم, جهان الحاتم, انوار الطائي

“…تم في هذا البحث دراسة المؤشر الوراثي الجزيئي للنبات المدروس البوثس Epipremnum aureum باستعمال تقنية تفاعل التضاعفي المتخصص Specific - PCR اذ تم مكاثرة قطع الDNA باستعمال الكت الموصى به Favor PrepTM (Genomic DNA Extraction Mini Kit) وتضمينها من خلال تفاعل البلمرة المتسلسل Polymerase Chain Reaction واظهرت نتيجة الكشف عند المستوى الجزيئي لجينوم اوراق نبات البوثس أن حجوم الحزم الناتجة كان مطابقاً لما جاء في تصميم زوج البوادئ (ITS1F: 5:TCCGTAGGTGAACCTGCGG-3)) (ITS4R:5:TCCTCCGCTTATTGATAGC-3) المسؤول كمحدد عن وجود المورث قيد الدراسة وظهرت الحزم اثناء الترحيل الكهربائي بحجم 473 زوج قاعدة , أدخلت تسلسلات القواعد النتروجينية في برنامج NCBI-BLAST وبرنامج MEGA 6.0 لبيان مدى تقاربها مع المورثات الموجودة والمسجلة في بنك الجينات Gene Bank . …”
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Association of Cytotoxic T Lymphocyte Antigen-4 Gene Polymorphisms with Psoriasis Vulgaris: A Case-Control Study in Turkish Population by Hatice Gül Dursun, Hüseyin Osman Yılmaz, Recep Dursun, Sevsen Kulaksızoğlu

“…Genotyping of SNPs (−318C>T, +49A>G, and CT60A>G) in CTLA-4 gene was performed using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) in 103 Pv patients and 102 controls. …”
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Investigation of some anaerobic bacteria in the sputum of cystic fibrosis patients and healthy people in Tehran, Iran by Homa Hamayeli, Ahya Abdi-Ali, Bahareh Attaran, Morvarid Shafiei, Mohammadreza Modaresi

“…The prevalence of common anaerobic bacteria in CF patients was investigated by Polymerase Chain Reaction (PCR). Results: The relationship between Bifidobacterium and Prevotella in both of healthy and CF groups, as well as the disease and Bifidobacterium (p = 0.0437) and disease and Prevotella (p = 0.0514), were significant. …”
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