Endocervical adenocarcinomas and HPV genotyping in an HIV endemic milieu – a retrospective study by Lucília Lovane, Gabriella Lillsunde Larsson, Satish Tulsidás, Carla Carrilho, Sören Andersson, Christina Karlsson

“…HPV detection was performed using a multi-methodological approach, including Anyplex II, in-house polymerase chain reaction (PCR), and chromogenic and fluorescent in situ hybridisation techniques. …”
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Correlation between Follicular Fluid Fatty Acids and Cell-Free Mitochondrial DNA in Women Undergoing Intra-Cytoplasmic Sperm Injections by Zainab M. Alawad, Hanan L. Al-Omary

“…The samples were assessed for fatty acids, utilizing gas chromatography, and for relatively cell-free mitochondrial DNA, real-time polymerase chain reaction (PCR) was used. Results: There was a strong significant positive correlation between follicular fluid margaric acid and follicular fluid relative to cell-free mitochondrial DNA, as the correlation coefficient was 0.869, and the P value was 0.025. …”
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Downregulation of HBx Restrains Proliferation, Migration, and Invasion of HepG2 Cells by Chaoqun Huang, Wei Liu, Xiaochuan Zhao, Libin Zhao, Fuxiang Wang

“…HBx and XB130 expression was detected by reverse transcription quantitative polymerase chain reaction (RT-qPCR) and Western blot. Overexpression of HBx and XB130 was found in liver cancer tissues and cells. …”
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Angiotensin-Converting Enzyme Gene Insertion/Deletion Polymorphism and Small Vessel Cerebral Stroke in Indian Population by Puttachandra Prabhakar, Tanima De, Dindagur Nagaraja, Rita Christopher

“…ACE I/D polymorphism was detected by polymerase chain reaction. Results. Hypertension was significantly more prevalent in the patient group and was associated with 6-fold increase in risk for stroke. …”
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The incidence of HLA-DQ2/DQ8 in Turkish children with celiac disease and a comparison of the geographical distribution of HLA-DQ by Ahmet Basturk, Reha Artan, Aygen Yilmaz

“…In our patients diagnosed with CD, in addition to tissue transglutaminase antibodies (anti-tTG), we also evaluated HLA-DQ2 B1 and HLA-DQ8 B1 alleles using the method of polymerase chain reaction (PCR)/sequence-specific oligonucleotide probes (Luminex®). …”
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A Universal Analysis Pipeline for Hybrid Capture-Based Targeted Sequencing Data with Unique Molecular Indexes by Min-Jung Kim, Si-Cho Kim, Young-Joon Kim

“…Unique molecular index (UMI) technology has recently been developed and helps to increase the accuracy of variant calling by minimizing polymerase chain reaction biases and sequencing errors. However, UMI-adopted targeted sequencing data analysis is slightly different from the methods for other types of omics data, and its pipeline for variant calling is still being optimized in various study groups for their own purposes. …”
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Identification and validation of reference genes for quantitative gene expression analysis under 409 and 415 nm antimicrobial blue light treatment by Beata Kruszewska-Naczk, Mariusz Grinholc, Aleksandra Rapacka-Zdonczyk

“…IntroductionReverse transcription quantitative real-time polymerase chain reaction Q7 (RT‒qPCR) is a commonly used tool for gene expression quantification. …”
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Study of the role of transmembrane emp24 domain-containing protein 2 in oral squamous cell carcinoma by RUAN Zhao-wei, JIAO Xue-feng, XIAO Gao-tian, CHEN Jin-ling, LI Jun, LV Shu-ying

“…The analysis results were further verified by assessing TMED2 expression levels in human normal oral keratinocyte cells and human OSCC cell lines using quantitative real-time polymerase chain reaction and the Western blot. Finally, the effects of TMED2 knockdown and overexpression on the expression levels of TMED2, ADP-ribosylation factor 1, extracellular signal-regulated kinase ½, and phospho-extracellular signal-regulated kinase ½ proteins were examined in cells using the Western blot. …”
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CDCA7 enhances STAT3 transcriptional activity to regulate aerobic glycolysis and promote pancreatic cancer progression and gemcitabine resistance by Dijie Zheng, Yazhu Deng, Lu Deng, Zhiwei He, Xinghao Sun, Yanyu Gong, Binbin Shi, Deqin Lu, Chao Yu

“…Western blotting, immunohistochemistry, and real-time polymerase chain reaction were performed to detect CDCA7 expression in PC cells and tissues. …”
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Molecular diagnosis and epidemiological aspects of cutaneous Leishmaniasis in Aleppo: Current status by Lana Kourieh, Mohammad Y. Abajy, Mahasen Alkebajy, Silva Ishkhanian, Ream Nayal

“…This study aimed to evaluate the effectiveness of PCR (polymerase chain reaction) in diagnosing suspected CL cases. …”
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Determination of FMDV 146S particle concentration by spectrometric method during viral RNA quantification by M. I. Doronin, D. V. Mikhalishin, V. A. Starikov, D. A. Lozovoy, Yu. S. El’kina, A. V. Borisov

“…The spectrometric method showed 94.5–99.5% correlation with real time reverse transcription polymerase chain reaction and complement fixation test based on the results of tests of 360 non-inactivated suspensions of FMDV of all types. …”
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Identification of Nontuberculous Mycobacterium and Mycobacterium tuberculosis Complex in Sputum Patients with Suspected Tuberculosis by Fanny Indriyani Biyang, Muhammad Nasrum Massi, Lisa Tenriesa Muslich, Andi Rofian Sultan, Mochammad Hatta, Ahmad Rahmat Ramadhan, Baedah Madjid

“…This study aims to identify mycobacterial species in sputum samples from patients suspected of having TB, utilizing polymerase chain reaction (PCR) assays and gene sequencing techniques. …”
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HMGB2 Deficiency Mitigates Abdominal Aortic Aneurysm by Suppressing Ang-II-Caused Ferroptosis and Inflammation via NF-κβ Pathway by Hao Wu, Legao Chen, Kaiping Lu, Yi Liu, Weiqin Lu, Jinsong Jiang, Chao Weng

“…Reverse transcription–quantitative polymerase chain reaction and western blot were utilized to assess HMGB2 levels. …”
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Hemophagocytic syndrome: Laboratory and molecular characterization by Nooran Salem Yaseen, Sadiq Khalaf Ali, Hussam Saadi Aziz

“…Whole blood was tested for complete blood count and screened for the presence of mutations in the perforin gene by polymerase chain reaction amplification; in addition, serum samples were tested for soluble CD25, ferritin, and triglycerides (TGs). …”
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Network Pharmacology Analysis and Experimental Validation to Explore the Anti-inflammatory Mechanism of Asiatic Acid on Alcoholic Steatohepatitis by Tao Jiang, Jinhong Xu, Yang Lu, Xiaojin Chen, Yongxia Li

“…In addition, in the cellular model, real-time reverse transcriptase-polymerase chain reaction (Real-Time RT-PCR) and enzyme-linked immunosorbent assay (ELISA) results showed that AA could alleviate alcohol-induced cellular inflammation, while western blot and immunofluorescence results showed that AA might alleviate alcohol-induced cellular inflammation by inhibiting the nuclear factor-κB (NF-κB) pathway. …”
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3p14 De Novo Interstitial Microdeletion in a Patient with Intellectual Disability and Autistic Features with Language Impairment: A Comparison with Similar Cases by Ana Belén de la Hoz, Hiart Maortua, Ainhoa García-Rives, María Jesús Martínez-González, Maitane Ezquerra, María-Isabel Tejada

“…An 8 Mb de novo interstitial deletion at 3p14.2-p14.1, from position 60.461.316 to 68.515.453, was revealed by means of array comparative genomic hybridization and confirmed using quantitative reverse-transcription polymerase chain reaction assays. This region includes six genes: FEZF2, CADPS, SYNPR, ATXN7, PRICKLE, and MAGI1, that are known to have a role in neurodevelopment. …”
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Role of ixodid ticks in tick-borne pathogen spread and circulation in the Belarusian Lakeland by A. A. Osmolovsky, I. A. Subotsina

“….), Babesia spp. and Tick-borne encephalitis virus genetic materials with real-time polymerase chain reaction using the reagent kit for nucleic acid extraction from environmental samples in accordance with the manufacturer’s instruc­tions. …”
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An Immunological and Molecular Study of Regulatory T-cell Activity in Iraqi Patients with Psoriasis Treated by Topical and Biological Therapy by Shawq Raafat Alnaqqash, Zainab Thamer Showait AL-Asady, Mohammed Mahdi Jawad

“…Materials and Methods: In this study 22 blood samples of psoriasis patients and 24 healthy samples as a control group were analyzed to measure the level of TGF-β1 by enzyme-linked immunosorbent assay and the gene expression level of the LRRC32 (GARP gene) by quantitative reverse transcription polymerase chain reaction technique. Results: The results showed a significant decrease (p=0.004) in the level of TGF-β1 in the serum of patients with psoriasis treated with topical therapy and others treated with biological therapy compared to the control group. …”
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Investigation of rs1746661 Polymorphism in FNDC5 Gene in Obese Patients by Elham Hadi, Hashem Nayeri, Ali Mohammad Ahadi, Ziba Rezvani Sichani

“…Specific primers for G/A/T alleles were designed for the amplification refractory mutation system-polymerase chain reaction (PCR) method, and PCR was performed. …”
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Early Magnetic Resonance Detection of Natalizumab-Related Progressive Multifocal Leukoencephalopathy in a Patient with Multiple Sclerosis by Guglielmo Manenti, Simone Altobelli, Marco Nezzo, Marco Antonicoli, Erald Vasili, Luca Neroni, Roberto Floris, Giovanni Simonetti

“…Diagnosis of progressive multifocal leukoencephalopathy is usually based on the clinical presentation, on the demonstration of the brain lesions at the magnetic resonance imaging examination, and on the detection of the JC virus DNA in the cerebrospinal fluid with high sensitive polymerase chain reaction. The role of magnetic resonance imaging specifically in natalizumab-associated progressive multifocal leukoencephalopathy is strengthening, and it is gaining importance not only as an irreplaceable diagnostic tool but also as a surveillance and risk stratifying tool in treated patients. …”
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