Circulation of Dengue Virus Serotypes in the City of Makkah, Saudi Arabia, as Determined by Reverse Transcription Polymerase Chain Reaction by Sameer R. Organji, Hussein H. Abulreesh, Gamal E. H. Osman

“…Blood samples were collected from 25 dengue fever-suspected patients and were subjected to molecular typing for DENV-1, DENV-2, DENV-3, and DENV-4 serotypes of dengue virus, by reverse transcription polymerase chain reaction (RT-PCR), using six sets of primers. …”
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Rapid detection of Vibrio alginolyticus in seafood using the flgL gene and real-time polymerase chain reaction by Muktiningsih Nurjayadi, Gladys Indira Putri, Jefferson Lynford Declan, Ismaya Krisdawati, Dandy Akbar Juliansyah, Maharanianska Azzahra, Irvan Maulana, Irma Ratna Kartika, Fera Kurniadewi, Tiara Fahriza, Adinda Myra Amalia Putri, Ayu Berkahingrum, Atikah Nur Rahmawati, Rosita Gio Anggraeni, Dalia Sukmawati, Sri Rahayu, Vira Saamia, I Made Wiranatha, Bassam Abomoelak, Hesham Ali El-Enshasy

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Differential Diagnosis of Entamoeba spp. in Clinical Stool Samples Using SYBR Green Real-Time Polymerase Chain Reaction by Thiago dos Santos Gomes, Mariana Coimbra Garcia, Flavia de Souza Cunha, Heloisa Werneck de Macedo, José Mauro Peralta, Regina Helena Saramago Peralta

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Exploration of different quantitative polymerase chain reaction-based genotyping methods to distinguish Apcmin/+ mice from wildtype mice. by Yuting Sun, Tingyu Zhou, Silin Ye, Effie Yin Tung Lau, Yao Zeng, Jessie Qiaoyi Liang

“…However, despite the widespread availability of quantitative polymerase chain reaction (qPCR) instruments in biomedical laboratories, the lack of professional analytical tools impedes the application of qPCR in genotyping. …”
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Phylogenetic and Genetic Variation Analysis of ITS1 Gene of Trypanosoma lewisi in Wild Rats Using Polymerase Chain Reaction by Reza Yesica, Andreas Bandang Hardian, Yudit Oktanella, Shelly Kusumarini, Gabriela Jeanne Giacynta Prasetyo

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Serological and Molecular Diagnosis of Human Cytomegalovirus among Hemodialysis Patients in Kirkuk/Iraq by Eman Ammar Ihsan, Lezan Medhat Mohammed, Waleed Mohammed Ali

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Linear Methods for Analysis and Quality Control of Relative Expression Ratios from Quantitative Real-Time Polymerase Chain Reaction Experiments by Robert B. Page, Arnold J. Stromberg

“…Relative expression quantitative real-time polymerase chain reaction (RT-qPCR) experiments are a common means of estimating transcript abundances across biological groups and experimental treatments. …”
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Development of polymerase chain reaction kit for detection of SARS-CoV-2 RNA in biological samples collected from animals by A. M. Timina, A. S. Yakovleva, M. V. Timanov, M. V. Biryuchenkova, Ye. S. Orlova

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A Multiplex Polymerase Chain Reaction Assay for the Detection of Herpes Simplex Virus, Cytomegalovirus, and Varicella-Zoster Virus in Cerebrospinal Fluid by Tatjana Luzius, Samuel D. Jeske, Julia Baer, Uta Goelnitz, Ulrike Protzer, Jochen M. Wettengel

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Positive real-time PCR in pneumococcal meningitis 12 hours after initiation of antibiotic therapy – case report by Cem Thunstedt, Carla Palleis, Johannes Wischmann, Suzette Heck, Konstantinos Dimitriadis, Matthias Klein

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Standardization and validation of a novel reverse transcriptase polymerase chain reaction method for detecting virulent strains of the infectious bursal disease virus by Vladimir Longa-Bobadilla, Phillip Ormeño-Vásquez, Manuel Criollo-Orozco, Luis Tataje-Lavanda, Katherine Huamán-Gutierrez, Ángela Montalván, Mirko Zimic, Manolo Fernández-Sanchez, Manolo Fernández-Díaz

Subjects: “…novel reverse transcriptase polymerase chain reaction…”
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Identification of Common Bacterial Pathogens Causing Meningitis in Culture-Negative Cerebrospinal Fluid Samples Using Real-Time Polymerase Chain Reaction by Walaa Shawky Khater, Safia Hamed Elabd

“…Forty culture-negative CSF samples from suspected cases of bacterial meningitis were examined by real-time polymerase chain reaction (real-time PCR) for the presence of lytA, bexA, and ctrA genes specific for Streptococcus pneumoniae, Haemophilus influenzae, and Neisseria meningitidis, respectively. …”
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Determination of Real-Time Polymerase Chain Reaction Uncertainty of Measurement Using Replicate Analysis and a Graphical User Interface with Fieller’s Theorem by James Ian Stuart, Johan Delport, Robert Lannigan, George Zahariadis

“…BACKGROUND: Disease monitoring of viruses using real-time polymerase chain reaction (PCR) requires knowledge of the precision of the test to determine what constitutes a significant change. …”
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METHOD FOR FMD VIRUS 146S COMPONENT CONCENTRATION DETERMINATION WITH REAL-TIME REVERSE TRANSCRIPTION – POLYMERASE CHAIN REACTION IN VACCINE RAW MATERIALS by M. I. Doronin, A. M. Timina, D. A. Lozovoy, V. A. Starikov, D. V. Mikhalishin, N. N. Medvedeva, A. V. Borisov

“…Method for determination of foot-and-mouth disease (FMD) virus 146S component concentration with real-time reverse transcription – polymerase chain reaction (rtRT-PCR) in vaccine raw materials is developed. …”
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Impact of viral load on sample pooling for reverse-transcription polymerase chain reaction detection-based diagnosis of coronavirus disease 2019 in Nigeria by Timan T. Eliya, Elvis E. Isere, Bassey Emmana, Chukwuebuka Ugwu, Jonathan Kushim, Precious Ishaku, Aisha E. Ibrahim, John S. Bimba

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Comparison of reverse-transcriptase polymerase chain reaction (rt-pcr) and rapid test for the detection of bovine rotavirus and bovine coronavirus in anatolian water by Oya Bulut, Gülşah Uyunmaz Saklı, Mustafa Hasöksüz, Irmak Dik, Hasan Hüseyin Hadimli, Mustafa Hitit

“…For this purpose, presence of these two viruses were investigated by Reverse Transcriptase Polymerase Chain Reaction (RT-PCR) and BRV-BCoV Rapid tests and sensitivity and specificity ratios of these two tests were compared.…”
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Clinical Presentation of Patients with Aseptic Meningitis, Factors Influencing Treatment and Hospitalization, and Consequences of Enterovirus Cerebrospinal Fluid Polymerase Chain Reaction Testing by Glenn Patriquin, Jill Hatchette, Kevin Forward

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Quantitative Detection of Beef and Beef Meat Products Adulteration by the Addition of Duck Meat Using Micro Drop Digital Polymerase Chain Reaction by Chen Chen, Jia Chen, Yan Zhang, Yongbo Li, Zan Wang, Xian Li, Wei Zhou, Zhisheng Zhang

“…A single-copy specific primer was designed based on beef and duck samples and through drop digital polymerase chain reaction (ddPCR) for the quantitative analysis. …”
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METHOD FOR FMD VIRUS 146S COMPONENT CONCENTRATION DETERMINATION WITH REAL-TIME REVERSE TRANSCRIPTION – POLYMERASE CHAIN REACTION IN VACCINE RAW MATERIALS by M. I. Doronin, A. M. Timina, D. A. Lozovoy, V. A. Starikov, D. V. Mikhalishin, N. N. Medvedeva, А. V. Borisov

“…Method for determination of foot-and-mouth disease (FMD) virus 146S component concentration with real-time reverse transcription – polymerase chain reaction (rtRT-PCR) in vaccine raw materials is developed. …”
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Detection of new single nucleotide polymorphisms in plasma cells carcinoma (myeloma) by polymerase chain reaction–single strand conformation polymorphism and sequencing technical methods by Sarah K. Obayes, Sabah H. Enayah, Shaimaa A. Al-Oubaidy, Rana A. Ghaleb

“…Genotyping of miRNA was accomplished using the polymorphism techniques of polymerase chain reaction and single strand conformation polymorphism (SSCP). …”
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