COVID-19 Outbreak among Healthcare Workers in a Specialty Unit in a Low-Middle Income Setting by Regina E. Oladokun, Babatunde O. Ogunbosi, Oreoluwa E. Morakinyo

“…Severe-acute-respiratory-syndrome-related coronavirus laboratory tests followed the World Health Organization recommendations, with nasopharyngeal swabs obtained for real-time reverse transcription–polymerase chain reaction. Results: Among the HCWs, a 49% COVID-19 positivity rate was found, with cases peaking in the third week. …”
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Laser Irradiation Alters the Expression Profile of Genes Involved in the Extracellular Matrix In Vitro by Sandra M. Ayuk, Nicolette N. Houreld, Heidi Abrahamse

“…Real-time reverse transcription (RT) quantitative polymerase chain reaction (qPCR) was used to determine the expression of 84 genes in a PCR array. …”
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Promyelocytic Leukemia with No Retinoic Acid Receptor Alpha Abnormality but with RUNX1T1 Insertion to Chromosome 7q: A Classification and Management Dilemma by Kathleen Overholt, Terri L. Guinipero, Nyla A. Heerema, Michael R. Loken, Samir B. Kahwash

“…Bone marrow examination showed morphologic and immunophenotypic findings typical of APL, but conventional cytogenetics, fluorescence in situ hybridization (FISH), and real-time polymerase chain reaction (RT-PCR) showed no evidence of RARA rearrangements. …”
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FV Leiden, Prothrombin II, and MTHFR C677T Mutations in Children and Young Adults with Thromboembolic Diseases by Sura O. AL-Dewachi, Wasem Alkafaf, Farah Mudhafar Fattah, Salman Haza, Muna A. Kashmoola

“…Detection of gene polymorphism was done in three steps started by isolation of DNA from the blood samples followed by in vitro polymerase chain reaction amplification then hybridization of amplification products at 45°C. …”
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Prevalence and Molecular Epidemiological Data on Dirofilaria immitis in Dogs from Northeastern States of India by Sonjoy Kumar Borthakur, Dilip Kumar Deka, Saidul Islam, Dilip Kumar Sarma, Prabhat Chandra Sarmah

“…Diagnostic methods applied were microscopy (wet film and Knott’s concentration technique), immunological test (Ag ELISA by SNAP 4Dx ELISA kit), and molecular tools (polymerase chain reaction and sequencing), which evidenced 11.38, 18.03, and 13.93% of positive animals, respectively. …”
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Expression and Secretion of CXCL-8 and CXCL-10 From <emph>Mycobacterium Bovis</emph> BCG-Infected Human Epithelial Cells: Role of IL-4

“…Gene expression of CXCL-8 and CXCL-10 was determined by the reverse transcription (RT)-polymerase chain reaction method. The levels of immunoreactive CXCL-8 and CXCL-10 were determined by enzyme-linked immunosorbent assay. …”
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Detection of Candida tropicalis from camel mastitis by S. A. Esmaeel, S. F. H. Al-Abedi, M. A. Altaliby, Q. T. Al-Obaidi

“…The present study was conducted to detect Candida tropicalis as a cause of camel mastitis (CM) us-ing routine diagnostic tests including Sabouraud’s dextrose agar (SDA) and API (analytical profile index) test strips, which were confirmed through amplification of the 18S rRNA gene of C. tropicalis using conventional polymerase chain reaction (c-PCR) technique and to investigate of the virulence genes of C. tropicalis including secreted aspartyl proteinase (SAPT4) and agglutinin-like sequence (ALST1) genes using c-PCR. …”

Homology Modeling and Expression of Recombinant NS5-RdRp Based on the Indonesian Local Strain of Dengue Virus for Anti-Dengue Drug Development by Toto Subroto, Ade Rizqi Ridwan Firdaus, Shinta Kusumawardani, Taufik Ramdani Tohari, Theresia Adventina Nababan, Wulan Pertiwi, Muhammad Fadhlillah, Sri Agung Fitri Kusuma, Yaya Rukayadi

“…We utilize reverse transcription- polymerase chain reaction (RT-PCR) and nested PCR for serotyping the DENV isolates, obtained from Hasan Sadikin Hospital, Indonesia. …”
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Two Novel 30K Proteins Overexpressed in Baculovirus System and Their Antiapoptotic Effect in Insect and Mammalian Cells by Wei Yu, Qing Li, Yuhua Yao, Yanping Quan, Yaozhou Zhang

“…Additionally, the cDNA sequences of both Slp and Lsp-t were obtained from the fat bodies of silkworm larvae by reverse transcription polymerase chain reaction. Both genes were expressed in BmN cells using the Bac-to-Bac system. …”
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Extracellular Vesicles Derived from Human Umbilical Cord Mesenchymal Stem Cells Promote Trophoblast Cell Proliferation and Migration by Targeting TFPI2 in Preeclampsia by Ying Chen, Chenchen Zhou, Xiaobo Zhao, Ronghua Che, Yuelin Wu, Sheng Wan, Jinda Pei, Liping Yao, Xiaolin Hua

“…The messenger ribonucleic acid (mRNA) levels of miR-195 and tissue factor pathway inhibitor 2 (TFPI2) were measured in placental tissues derived from normal and preeclampsia patients by real-time polymerase chain reaction (PCR). Human umbilical cord mesenchymal stem cell (hUC-MSC)-derived extracellular vesicles were verified by western blot. …”
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Identifying preeclampsia-associated key module and hub genes via weighted gene co-expression network analysis by Jie Li, Lingling Jiang, Haili Kai, Yang Zhou, Jiachen Cao, Weichun Tang

“…Finally, hub genes were selected via Venn Plot and confirmed by quantitative real-time polymerase chain reaction. Through the co-expression networks and modules, we ensured the turquoise module was the most significant one related to PE. …”
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Artificial Neural Network-Based Identification of Associations between UCP2 and UCP3 Gene Polymorphisms and Meat Quantity Traits by Javed Ahmed Ujjan, Sapna Waheed Memon, Majeeda Ruk, Zuneera Akram, Allahwadhayo Ghoto, Betty Nokobi Dugbakie

“…For better analysis, the output of the ANN model is loaded into the Primer Premier 3 software. Using polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) and sequencing, the results of this study indicated 07 variations in the exon 4 region of the UCP2 gene and 03 variants in the exon 3 area of the UCP3 gene among 215 indigenous cow breeds. …”
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Identification of Mutations that Encode Drug Resistance in the Polymerase Gene of the Human Immunodeficiency Virus by Zhengxian Gu, Hengsheng Fang, Horacio Salomon, Qing Gao, Mark A Wainberg

“…The complete reverse transcriptase open reading frames of these viruses, and portions of flanking protease and integrase within the pol gene, were cloned and sequenced by polymerase chain reaction (PCR) techniques. Mulalions were observed at each of amino acid sites 65 (Lys → Arg: AAA → AGA) and 184 (Met → Val: ATG → GTG) when ddC was used in this protocol, and at site 184 only when either 3TC or ddl was employed. …”
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The Effect of Clopidogrel and Ticagrelor on Human Adipose Mesenchymal Stem Cell Osteogenic Differentiation Potential: In Vitro Comparative Study by Sally S. Mohamed, Hala F. Zaki, Shereen N. Raafat

“…Quantitative determination for osteogenic markers included osteocalcin (OC); runt-related transcription factor 2 (RUNX2) performed using western blot; osteoprotegerin (OPG) using enzyme-linked immunosorbent assay (ELISA) and inflammatory markers; and tumor necrosis factor (TNF-α) and interleukin-6 (IL-6) measured using real-time polymerase chain reaction quantitative (RT-PCR) and ELISA. …”
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RNA-binding protein DAZAP1 accelerates the advancement of pancreatic cancer by inhibiting ferroptosis by Xinqing Wang, Hao Fan, Xiaoping Ye, Yu Hu, Yan Xiao, Ming Zhang, Yonghui Xu, Jianjun Song, Yongyun Luo

“…Methods DAZAP1 expression in PC tissues, normal tissues and cell lines was assessed using immunohistochemistry (IHC), reverse transcription–quantitative polymerase chain reaction (RT–qPCR) and western blotting. DAZAP1 knockdown was achieved through plasmid transfection, and its effects on ferroptosis and PC progression were evaluated using RT–qPCR, western blotting, CCK-8 assays, EdU staining, Fe2+ content measurement, reactive oxygen species (ROS) detection, wound healing and Transwell migration assays. …”
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Expression Profiles of 12 Late Embryogenesis Abundant Protein Genes from Tamarix hispida in Response to Abiotic Stress by Caiqiu Gao, Yali Liu, Chao Wang, Kaimin Zhang, Yucheng Wang

“…The expression profiles of these genes in response to NaCl, PEG, and abscisic acid (ABA) in roots, stems, and leaves of T. hispida were assessed using real-time reverse transcriptase-polymerase chain reaction (RT-PCR). These ThLEAs all showed tissue-specific expression patterns in roots, stems, and leaves under normal growth conditions. …”
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Application of Next-Generation Sequencing Technology Based on Single Gene Locus in Species Identification of Mixed Meat Products by Xinmei Liu, Zhiyang Liu, Yiyu Cheng, Haijing Wu, Wei Shen, Yan Liu, Qiushi Feng, Jun Yang

“…Polymerase chain reaction (PCR) detection is a commonly used method for species identification of meat products. …”
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Molecular Characteristics and Antibiotic Resistance Profiles of Klebsiella Isolates in Mthatha, Eastern Cape Province, South Africa by Sandeep Vasaikar, Larry Obi, Isaac Morobe, Mary Bisi-Johnson

“…The cultured Klebsiella isolates were subjected to antimicrobial susceptibility tests and the polymerase chain reaction of blaCTX-M, blaTEM, blaSHV, blaKPC, and blaNDM genes. …”
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Investigations on the Effects of Dietary Essential Oils and Different Husbandry Conditions on the Gut Ecology in Piglets after Weaning by P. Janczyk, R. Pieper, V. Urubschurov, K. R. Wendler, W. B. Souffrant

“…Genomic DNA was extracted from digesta and polymerase chain reaction—denaturing gradient gel electrophoresis was performed. …”
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Comparison of Prevalence of blaNDM-1 Gene among Metallo-Beta Lactamase-Producing Multidrug-Resistant Fermenters and Nonfermenters in a Tertiary Care Hospital by Shaveta Kataria, Shipra Galhotra, Mohit Kumar, Priyanka Thandi, Neerja Jindal

“…Combined disk synergy test (CDST) and polymerase chain reaction (PCR) analysis are used for the detection of MBL production. …”
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