Role of circRMRP and circRPL27 in chronic obstructive pulmonary disease by Li JianFang, Zhang PengFei, Zeng XianJing, Liu Rong

“…Quantitative real-time polymerase chain reaction technology was used to determine the expression of circRPL27 and circRMRP in COPD. …”
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Improvement of Skin Wound Healing for Diabetic Mice with Thermosensitive Hydrogel Combined with Insulin Injection by Lingling Fang, Haijian Wu, Xiaoyan Li, Jianghua Fang, Yabin Zhu

“…Real-time quantitative polymerase chain reaction (qRT-PCR) was performed to measure the relative expression of α-smooth muscle actin (α-SMA) and transforming growth factor beta 1 (TGF-β1) in wound tissues at 7, 14, and 28 days. …”
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HSV-1 Encephalitis: High Index of Clinical Suspicion, Prompt Diagnosis, and Early Therapeutic Intervention Are the Triptych of Success—Report of Two Cases and Comprehensive Review... by Dimitrios Patoulias, Georgios Gavriiloglou, Konstantinos Kontotasios, Mairi Tzakri, Petros Keryttopoulos, Christos Koutras

“…It should be noted that even when Cerebrospinal fluid (CSF) analysis seems normal, imaging studies are not specific and HSV Polymerase Chain Reaction (PCR) test is negative; the clinician should be more aggressive, if clinical presentation is indicative for HSV encephalitis, by administrating acyclovir early after patient’s admission. …”
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Over-the-counter carrageenan-based sprays may interfere with PCR testing of nasopharyngeal swabs to detect SARS-CoV-2. by Taylor Corocher, Kira Edwards, Yvonne Hersusianto, Donald Campbell, Hui Yin Lim, Paul Monagle, Prahlad Ho

“…Carrageenan belongs to the glycosaminoglycan family alongside heparin, and heparin is known to inhibit real-time quantitative polymerase chain reaction (RT-qPCR) in nasopharyngeal swabs used to detect SARS-CoV-2. …”
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Investigation on Prevalence of Canine Trypanosomiasis in the Conservation Areas of Bwindi-Mgahinga and Queen Elizabeth in Western Uganda by James Robert Ochieng, Marta Planellas Bachs, Anthony Nsubuga, Innocent B. Rwego, John Joseph M. Kisakye, Laura Muro Riba, Jesus Muro Figueres

“…Trypanosome tests using microhematocrit centrifugation/dark ground microscopy technique (MHCT) followed by conventional polymerase chain reaction (cPCR) were performed in blood samples collected from identified indigenous dogs (n=124). …”
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Decision Tree Ensembles to Predict Coronavirus Disease 2019 Infection: A Comparative Study by Amir Ahmad, Ourooj Safi, Sharaf Malebary, Sami Alesawi, Entisar Alkayal

“…The detection of Covid-19 positive cases is an important step to fight the pandemic and save human lives. The polymerase chain reaction test is the most used method to detect Covid-19 positive cases. …”
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Marker-Assisted Selection for Recognizing Wheat Mutant Genotypes Carrying HMW Glutenin Alleles Related to Baking Quality by Mohammad Javad Zamani, Mohammad Reza Bihamta, Behnam Naserian Khiabani, Zahra Tahernezhad, Mohammad Taher Hallajian, Marzieh Varasteh Shamsi

“…Marker-assisted selection is the method which can recognize the alleles related to baking quality and this method is based on polymerase chain reaction. 10 pairs of specific primers related to Dx2, Dx2.1, Dx5, Dy10, and Dy12 subunits were used for recognizing baking quality of some wheat varieties and some mutant genotypes. …”
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Association of Lumican Gene with Susceptibility to Pathological Myopia in the Northern Han Ethnic Chinese by Fengju Zhang, Tingzhun Zhu, Zhongjun Zhou, Yudong Wu, Yang Li

“…Genotyping was performed by direct sequencing after polymerase chain reaction(PCR) amplification and allele frequencies were tested for Hardy-Weinberg equilibrium. …”
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Epidemiology and Molecular Aspect of pediculosis among Primary School Children in Sulaimani Province Kurdistan-Iraq by Abdullah A. Haama

“…Direct contact, Sharing of clothing and combs can be a way for lice transmission from infected person to another.This study had been done to determine the pediculosis infection rate and risk factors asociassion with head lice among primary schools children in Sulaimani Province, all cases were evaluated by physical examination (visual inspection) and detail structured questionnaire which was including education levels, family size, hair washing frequency, type and length of hair, mother occupation and education, father occupation, and education was used, the polymerase chain reaction (PCR) used for confirming the head lice clade. …”
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Drug response is related to NR3C1 and FAAH polymorphism in Chinese pediatric epilepsy patients by Hongli Wang, Chu Li, Qian Li, Ning Li, Huiling Qin

“…Methods NR3C1 rs41423247 and FAAH rs324420 polymorphisms were detected by the polymerase chain reaction in 105 pediatric epilepsy patients. …”
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Markers of chronic hepatitis B at patients with HIV-infection and treatment approaches by S. I. Maksimov, S. P. Tsarenko, A. V. Kravchenko, N. Yu. Gankina, N. D. Yushchuk

“…All blood samples were investigated for the presence of НBV, HDV, HCV, HAV-virus markers in serum by enzyme-linked immunoassay and for the presence of RNA HIV, RNA HCV and DNA HBV by polymerase chain reaction.Results. It was revealed, that CHB markers are detected approximately at 5,5–6,8% of patients with HIV-infection, in most of the cases in combination to CHC or CHD markers. …”
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Experiences of Emergency Surgical Treatment for a COVID-19 Patient with Severe Traumatic Brain Injury at a Regional Trauma Center: A Case Report by Jung-Ho Yun

“…The initial COVID-19 test administered upon presentation to the emergency room had a positive result, and a confirmatory polymerase chain reaction (PCR) test was administered. The PCR test confirmed a positive result. …”
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Endometriosis-Associated Massive Ascites in an Asian Woman: A Case Report of a Rare Clinical Entity by Nuntasiri Eamudomkarn, Naratassapol Likitdee, Pilaiwan Kleebkaow, Chumnan Kietpeerakool

“…Abdominal paracentesis was performed to yield fluid samples for evaluation, which revealed no malignant cells, and polymerase chain reaction (PCR) was negative for tuberculosis. …”
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Lymphangiogenesis in Oral Squamous Cell Carcinoma: Correlation with VEGF-C Expression and Lymph Node Metastasis by Manar A. Abdul-Aziz, Amina K. Amin, Dalia H. El-Rouby, Olfat G. Shaker

“…The expression of VEGF-C was also assessed using immunohistochemistry and real-time polymerase chain reaction in 6 normal oral mucosa cases and 72 cases of OSCC. …”
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Interleukin-16 Polymorphism Is Associated with an Increased Risk of Ischemic Stroke by Xiao-li Liu, Jian-zong Du, Yu-miao Zhou, Qin-fen Shu, Ya-guo Li

“…A total of 198 patients with ischemic stroke and 236 controls were genotyped using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and DNA sequencing method. …”
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Molecular Determination of Toxocara spp. Eggs Isolated from Public Parks and Playgrounds in Zahedan, Southeast Iran by Siavash Liravizadeh, Samaneh Abdolahi Khabisi, Alireza Salimi Khorashad, Hadi Mirahmadi

“…The modified Sheather’s flotation technique was employed to isolate Toxocara spp. eggs from the soil, followed by microscopic assessment and molecular evaluation of internal transcribed spacer 1 and 2 ribosomal deoxyribonucleic acid (ITS1 and 2 rDNA) using nested polymerase chain reaction (nested PCR) to identify the presence of Toxocara spp. eggs. …”
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The Frequency of Intestinal Parasitic Infections in COVID-19 Patients: A Case-Control Study in Tehran, Capital of Iran by Ali Taghipour, Majid Pirestani, Ramin Hamidi Farahani, Mohammad Barati

“…RNA extraction was performed, and then real time reverse-transcription polymerase chain reaction (rRT-PCR) assay was applied to detect viral RNA. …”
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Seroprevalence of Hepatitis C, Hepatitis B, Cytomegalovirus, and Human Immunodeficiency Viruses in Multitransfused Thalassemic Children in Upper Egypt by Ramadan A. Mahmoud, Abdel-Azeem M. El-Mazary, Ashraf Khodeary

“…It is therefore important to implement measures to improve blood transfusion screening, such as polymerase chain reaction, in order to reduce TTIs from blood donor units.…”
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Molecular Identification Based on β-Tubulin of Trichomonas vaginalis Infection among Women in Babylon Province by Zainab Waddah Kermasha, Hayam Khalis Al-Masoudi, Suhaila Fadhil Mohammed

“…Objectives: This study was performed to utilize polymerase chain reaction (PCR) assays as a diagnostic tool for T. vaginalis. …”
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Analysis of a Functional IL-6 Gene Polymorphism in HLAB27 Associated and Intermediate Uveitis Gives New Insight in Disease Pathogenesis and Commonality with Other Autoimmune Diseas... by Lindner Ewald, Langner-Wegscheider Beate, Sarny Stephanie, Renner Wilfried, El-Shabrawi Yosuf

“…Main outcome measures were genotype distribution and allelic frequencies determined by polymerase chain reaction. Results. The frequency of carriers of the minor allele for rs1800795 was significantly higher in patients with intermediate uveitis compared to controls (p=0.04; OR: 1.46; CI: 1.02–2.11). …”
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