Prevalence of feline viral leukemia in Moscow and the Moscow Oblast with the analysis of hematological and biochemical blood parameters by A. P. Zenchenkova

“…Despite the statistical records that have been successfully kept in other countries for many years, there is little data on the prevalence of the infection in the Russian Federation, and the samples are represented by a small number of animals. 11,807 blood samples collected in Moscow and the Moscow Oblast were tested for FeLV antigen and antibodies against FeLV by polymerase chain reaction, enzyme immunoassay, and immunochromatographic assay. …”
Get full text

Use of Molecular Methods for the Rapid Mass Detection of Schistosoma mansoni (Platyhelminthes: Trematoda) in Biomphalaria spp. (Gastropoda: Planorbidae) by Roberta Lima Caldeira, Liana Konovaloffi Jannotti-Passos, Omar Dos Santos Carvalho

“…The low stringency-polymerase chain reaction (LS-PCR) and loop-mediated isothermal amplification (LAMP) assays were used to detect the presence of S. mansoni DNA in (1) Brazilian intermediate hosts (Biomphalaria glabrata, B. straminea, and B. tenagophila) with patent S. mansoni infections, (2) B. glabrata snails with prepatent S. mansoni infections, (3) various mixtures of infected and noninfected snails; and (4) snails infected with other trematode species. …”
Get full text

Segmentasi Citra X-Ray Dada Menggunakan Metode Modifikasi Deeplabv3+ by Rima Tri Wahyuningrum, Maughfirotul Jannah, Budi Dwi Satoto, Amillia Kartika Sari, Anggraini Dwi Sensusiati

“…Reserve Transcription Polymerase Chain Reaction (RT-PCR) merupakan alat yang paling banyak digunakan untuk mendeteksi COVID-19. …”
Get full text

Peroxisome Proliferator-Activated Receptor γ Regulates the Expression of Lipid Phosphate Phosphohydrolase 1 in Human Vascular Endothelial Cells by Yazi Huang, Beilei Zhao, Yahan Liu, Nanping Wang

“…In human umbilical vein endothelial cells (HUVECs), quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) demonstrated that activation of PPARγ increased the mRNA level of LPP1. …”
Get full text

The role of polymorphism (rs1800566) of NAD(F)H quinone oxidoreductase-1 in development of acute pancreatitis by T. A. Samgina, Y. V. Kanishchev, S. N. Grigor’Yev, P. M. Nazarenko, A. V. Polonikov

“…Genotyping of NQO1 gene polymorphism (rs1800566) was carried out by real-time polymerase chain reaction. Results. The association of polymorphism (rs1800566) of NQO1 gene T/T genotype with high risk of AP development, mostly - acute and non-biliary pancreatitis, in males. …”
Get full text

Molecular Detection of Pap II, OmpA, and LuxR Genes Responsible for Biofilm Formation in Acinetobacter baumannii Isolated from Hospitalized Patients by Estabraq Ali Maklef, Amal A. Kareem, Susan F. K. Al-Sudani

“…The isolates from intensive care unit (ICU) patients had strong, moderate, weak, and nonforming biofilm ability in higher rates of biofilm producers compared with the isolates from samples of hospital wards. The polymerase chain reaction (PCR) products showed genotypically positive results as follows: PapII 12 (31.5%), OmpA 11 (28.9%), and LuxR 8 (21%) out of 38 positive samples of A. baumannii for all genes. …”
Get full text

Identifying clinical phenotypes for hospitalized patients with chronic obstructive pulmonary disease acute exacerbation by Mayar Mamdoh, Mohamed A.H. El Nady, Hebatallah H. Assal, Gihan Saad, Amira I.A. Eldin, May S. Soliman, Amani A. Elkholy, Sara E.E. Haddad, Noha S. Soliman, Gehan Hamdy

“…Each patient was subjected to full history taking, clinical examination, plain chest radiography, bacterial culture, viral polymerase chain reaction, modified medical research council dyspnea scale, and COPD assessment test. …”
Get full text

Diagnostic Role of Plasma MicroRNA-21 in Stable and Unstable Angina Patients and Association with Aging by Md Sayed Ali Sheikh

“…Plasma miR-21 expression levels were determined by real-time polymerase chain reaction. To evaluate the diagnosis accuracy, the receiver operating characteristic (ROC) curves were used. …”
Get full text

Hsa_circ_0000190 Promotes NSCLC Cell Resistance to Cisplatin via the Modulation of the miR-1253/IL-6 Axis by Hua He, Tian Li

“…Analyses of NSCLC expression of hsa_circ_0000190, miR-1253, and interleukin 6 (IL-6) were conducted via a quantitative real-time polymerase chain reaction (qPCR) approach, while the ability of these tumor cells to resist DDP treatment was evaluated with a CCK-8 assay. …”
Get full text

Imaging Atherosclerotic Plaque Inflammation via Folate Receptor Targeting Using a Novel F-Folate Radiotracer by Adrienne Müller, Katharina Beck, Zoran Rancic, Cristina Müller, Cindy R. Fischer, Thomas Betzel, Philipp A. Kaufmann, Roger Schibli, Stefanie D. Kramer, Simon M. Ametamey

“…Increased FR-β and CD68 levels were found in atherosclerotic plaques compared to normal artery walls by quantitative real-time polymerase chain reaction. Western blotting and immunohistochemistry demonstrated prominent FR-β protein levels in plaques. …”
Get full text

A Case of Early Disseminated Neurological Lyme Disease Followed by Atypical Cutaneous Manifestations by Vamsi Kantamaneni, Vikas Sunder, Mohammad Bilal, Scott Vargo

“…The patient’s symptoms including facial droop resolved within 24 hours of antibiotic therapy. Polymerase chain reaction of the blood, IgM ELISA, and IgM Western blot testing for LD came back positive a few days after initiation of therapy. …”
Get full text

MicroRNA Regulation for Inflammasomes in High Glucose-Treated ARPE-19 Cells by Ji Hong Kim, Hyoseon Yu, Ji Hye Kang, Eun Hee Hong, Min Ho Kang, Mincheol Seong, Heeyoon Cho, Yong Un Shin

“…The expression levels of five miRNAs (miR-185, miR-17, miR-20a, miR-15a, and miR-15b) were measured in high glucose-treated ARPE-19 cells using real-time quantitative polymerase chain reaction. Western blotting was performed to measure inflammasome expression in cellular models. miR-17 was selected as the target miRNA, and inflammasome expression was measured following the transfection of an miR-17 mimic into high glucose-treated ARPE-19 cells. …”
Get full text

A Cytokine That Is Involved in Immune Responses in Chronic Hepatitis B Virus Patients by Noor R. Abady, Zeana Shakir Al-Al Hindi

“…Materials and Methods: There were four groups in the study: asymptomatic HBsAg carriers (n = 42), chronic hepatitis B (CHB, n = 57), liver cirrhosis (LC, n = 59), and the normal control group (n = 80). an Enzyme-Linked was used to measure the amounts of IL-17, while reverse transcription polymerase chain reaction was used to find IL-17 mRNA in peripheral blood mononuclear cells (PBMC). …”
Get full text

Polymorphisms of ATG5 Gene Are Associated with Autoimmune Thyroid Diseases, Especially Thyroid Eye Disease by Wen Wang, Zheng-yao Yu, Rong-hua Song, Shuang-tao He, Liang-feng Shi, Jin-an Zhang

“…Their frequencies in 824 AITD patients, including 271 HT patients and 553 GD patients, and 764 healthy controls were tested using both ligase detection reaction and multiplex polymerase chain reaction. Results. Allele A frequency of rs6568431 in AITDs patients (p=0.016, OR=1.201, 95% CI=1.034−1.394) and allele G frequency of rs6937876 in AITDs patients (p=0.009, OR=1.223, 95% CI=1.052−1.422) and in GD patients (p=0.009, OR=1.247, 95% CI=1.056−1.473) were significantly higher than those in the healthy controls. …”
Get full text

Surgical treatment of hepatic Echinococcus granulosus by Waldemar Patkowski, Maciej Krasnodębski, Michał Grąt, Łukasz Masior, Marek Krawczyk

“…Blood samples where used to proceed sequential enzyme-linked immunosorbent assay (ELISA) using Em2plus antigen as well as polymerase chain reaction (PCR) to detect E. granulosus . …”
Get full text

DNA MITOKONDRIA (mtDNA) SEBAGAI SALAH SATU PEMERIKSAAN ALTERNATIF UNTUK IDENTIFIKASI BAYI PADA KASUS INFANTISIDA by Taufik Hidayat

“…Perbandingan antara sampel DNA bayi dengan sampel DNA tersangka ibu menggunakan metode sekuensing PCR (Polymerase Chain Reaction). Pengambilan kesimpulan akhir pada pemeriksaan hubungan keibuan pada mtDNA harus dikombinasi dengan pemeriksaan forensik lainnya.…”
Get full text

Unveiling the molecular and comorbidity profile among transfusion-dependent and nontransfusion-dependent beta-thalassemic patients in Baghdad city by Atyab Abdulhameed Alaqidi, Hind Shaker Al-Mamoori

“…PATIENTS, MATERIALS AND METHODS: The molecular profile of HBB gene of 80 transfusion-dependent (TD) and non-TD BT patients from Baghdad thalassemia centers was examined using multiplex polymerase chain reaction and reverse hybridization technique and direct gene sequencing. …”
Get full text

Quantification and discovery of PCR inhibitors found in food matrices commonly associated with foodborne viruses by Cassandra Suther, Matthew D. Moore

“…Detection and quantification of norovirus commonly involves the use of reverse transcriptase quantitative polymerase chain reaction (RT-qPCR); however, the presence of inhibitory compounds in foods limit detection and accurate quantification. …”
Get full text

Characterization of Mating Type Genes in Aspergillus flavus Populations from Two Locations in Kenya by Ouko Abigael, Okoth Sheila, Amugune Nelson, Vesa Joutsjoki

“…Forty-four isolates from Nandi and Makueni counties were genotyped by MAT using a multiplex polymerase chain reaction assay. The primer pair for the MAT1-1 amplified a 396 base pair (bp) fragment containing an α-box motif, and MAT1-2 primers targeted a 270 bp segment with a high mobility group protein. …”
Get full text

A Canadian Isolate of Hepatitis D (delta) Virus by Véronique Poisson, Daniel B Ménard, Éric Frost, Jean-Pierre Perreault

“…The viral RNA from serum was amplified using reverse transcription coupled to polymerase chain reaction amplification. The resulting complementary DNA was cloned and sequenced. …”
Get full text